Effect of dietary L-theanine supplementation on skeletal muscle fiber type transformation in vivo
The aim of this study was to investigate the effect of dietary L-theanine supplementation on skeletal muscle fiber type transition in mice. Our data indicated that dietary 0.15% L-theanine supplementation significantly increased the mRNA expression levels of muscle fiber type related genes (MyHC I,...
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| Veröffentlicht in: | The Journal of nutritional biochemistry 2022-01, Vol.99, p.108859-108859, Article 108859 |
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| container_title | The Journal of nutritional biochemistry |
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| creator | Chen, Xiaoling Zhang, Man Xue, Yonghong Liang, Dahui An, Wenting Jia, Gang Zhao, Hua Liu, Guangmang Huang, Zhiqing |
| description | The aim of this study was to investigate the effect of dietary L-theanine supplementation on skeletal muscle fiber type transition in mice. Our data indicated that dietary 0.15% L-theanine supplementation significantly increased the mRNA expression levels of muscle fiber type related genes (MyHC I, MyHC IIa, PGC-1α, Sirt1, Tnnt1, Tnnc1, Tnni1, MEF2C) and the protein expression levels of MyHC IIa, myoglobin, PGC-1α, Sirt1 and Troponin I-SS, but significantly decreased the mRNA and protein expression levels of MyHC IIb. Dietary 0.15% L-theanine supplementation significantly increased the activities of SDH and MDH and decreased the activity of LDH. Furthermore, immunofluorescence demonstrated that dietary 0.15% L-theanine supplementation significantly increased the percentage of type I fibers, and significantly decreased the percentage of type II fibers. In addition, we found that dietary 0.15% L-theanine supplementation increased the fatigue-resistant, antioxidant capacity, mitochondrial biogenesis, and function in skeletal muscle of mice. Furthermore, dietary 0.15% L-theanine supplementation significantly increased the mRNA levels of prox1, CaN and NFATc1, the protein levels of prox1, CNA and NFATc1 and the activity of CaN in GAS muscle when compared with the control group. These results indicated that dietary L-theanine supplementation promoted skeletal muscle fiber transition from type II–type I, which might be via activation of CaN and/or NFATc1 signaling pathway. |
| doi_str_mv | 10.1016/j.jnutbio.2021.108859 |
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Our data indicated that dietary 0.15% L-theanine supplementation significantly increased the mRNA expression levels of muscle fiber type related genes (MyHC I, MyHC IIa, PGC-1α, Sirt1, Tnnt1, Tnnc1, Tnni1, MEF2C) and the protein expression levels of MyHC IIa, myoglobin, PGC-1α, Sirt1 and Troponin I-SS, but significantly decreased the mRNA and protein expression levels of MyHC IIb. Dietary 0.15% L-theanine supplementation significantly increased the activities of SDH and MDH and decreased the activity of LDH. Furthermore, immunofluorescence demonstrated that dietary 0.15% L-theanine supplementation significantly increased the percentage of type I fibers, and significantly decreased the percentage of type II fibers. In addition, we found that dietary 0.15% L-theanine supplementation increased the fatigue-resistant, antioxidant capacity, mitochondrial biogenesis, and function in skeletal muscle of mice. Furthermore, dietary 0.15% L-theanine supplementation significantly increased the mRNA levels of prox1, CaN and NFATc1, the protein levels of prox1, CNA and NFATc1 and the activity of CaN in GAS muscle when compared with the control group. These results indicated that dietary L-theanine supplementation promoted skeletal muscle fiber transition from type II–type I, which might be via activation of CaN and/or NFATc1 signaling pathway.</description><identifier>ISSN: 0955-2863</identifier><identifier>EISSN: 1873-4847</identifier><identifier>DOI: 10.1016/j.jnutbio.2021.108859</identifier><identifier>PMID: 34517095</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; CaN and/or NFATc1 signaling pathway ; Dietary Supplements - analysis ; Fiber type transition ; Gene Expression ; Glutamates - metabolism ; L-theanine ; Male ; Mice ; Muscle Fibers, Skeletal - metabolism ; Muscle Proteins - genetics ; Muscle Proteins - metabolism ; Muscle, Skeletal - metabolism ; Organelle Biogenesis ; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - genetics ; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - metabolism ; Sirtuin 1 - genetics ; Sirtuin 1 - metabolism ; Skeletal muscle</subject><ispartof>The Journal of nutritional biochemistry, 2022-01, Vol.99, p.108859-108859, Article 108859</ispartof><rights>2021</rights><rights>Copyright © 2021. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-8e00832962048ac6dcf6b6855095c280e6befbea17b4955d24cb08846db759593</citedby><cites>FETCH-LOGICAL-c365t-8e00832962048ac6dcf6b6855095c280e6befbea17b4955d24cb08846db759593</cites><orcidid>0000-0001-5092-9297</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jnutbio.2021.108859$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34517095$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Xiaoling</creatorcontrib><creatorcontrib>Zhang, Man</creatorcontrib><creatorcontrib>Xue, Yonghong</creatorcontrib><creatorcontrib>Liang, Dahui</creatorcontrib><creatorcontrib>An, Wenting</creatorcontrib><creatorcontrib>Jia, Gang</creatorcontrib><creatorcontrib>Zhao, Hua</creatorcontrib><creatorcontrib>Liu, Guangmang</creatorcontrib><creatorcontrib>Huang, Zhiqing</creatorcontrib><title>Effect of dietary L-theanine supplementation on skeletal muscle fiber type transformation in vivo</title><title>The Journal of nutritional biochemistry</title><addtitle>J Nutr Biochem</addtitle><description>The aim of this study was to investigate the effect of dietary L-theanine supplementation on skeletal muscle fiber type transition in mice. Our data indicated that dietary 0.15% L-theanine supplementation significantly increased the mRNA expression levels of muscle fiber type related genes (MyHC I, MyHC IIa, PGC-1α, Sirt1, Tnnt1, Tnnc1, Tnni1, MEF2C) and the protein expression levels of MyHC IIa, myoglobin, PGC-1α, Sirt1 and Troponin I-SS, but significantly decreased the mRNA and protein expression levels of MyHC IIb. Dietary 0.15% L-theanine supplementation significantly increased the activities of SDH and MDH and decreased the activity of LDH. Furthermore, immunofluorescence demonstrated that dietary 0.15% L-theanine supplementation significantly increased the percentage of type I fibers, and significantly decreased the percentage of type II fibers. In addition, we found that dietary 0.15% L-theanine supplementation increased the fatigue-resistant, antioxidant capacity, mitochondrial biogenesis, and function in skeletal muscle of mice. Furthermore, dietary 0.15% L-theanine supplementation significantly increased the mRNA levels of prox1, CaN and NFATc1, the protein levels of prox1, CNA and NFATc1 and the activity of CaN in GAS muscle when compared with the control group. These results indicated that dietary L-theanine supplementation promoted skeletal muscle fiber transition from type II–type I, which might be via activation of CaN and/or NFATc1 signaling pathway.</description><subject>Animals</subject><subject>CaN and/or NFATc1 signaling pathway</subject><subject>Dietary Supplements - analysis</subject><subject>Fiber type transition</subject><subject>Gene Expression</subject><subject>Glutamates - metabolism</subject><subject>L-theanine</subject><subject>Male</subject><subject>Mice</subject><subject>Muscle Fibers, Skeletal - metabolism</subject><subject>Muscle Proteins - genetics</subject><subject>Muscle Proteins - metabolism</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Organelle Biogenesis</subject><subject>Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - genetics</subject><subject>Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - metabolism</subject><subject>Sirtuin 1 - genetics</subject><subject>Sirtuin 1 - metabolism</subject><subject>Skeletal muscle</subject><issn>0955-2863</issn><issn>1873-4847</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v3CAQhlGVqLtJ-xNacczFW4wNxqcqivIlrdRLekaAB5WtDS7glfbfh8jbXCMhjQTPzPA-CH2rya4mNf9x2B38krULO0poXe6EYP0ntK1F11StaLsLtCU9YxUVvNmgq5QOhBDaMv4ZbZqW1V153SJ1by2YjIPFg4Os4gnvq_wHlHcecFrmeYQJfFbZBY_LSX9hLNyIpyWZEbB1GiLOpxlwjsonG-K0ws7jozuGL-jSqjHB13O9Rr8f7l_unqr9r8fnu9t9ZRrOciWAENHQnlPSCmX4YCzXXDBWvmmoIMA1WA2q7nRbYg20NbpkbvmgO9azvrlGN-vcOYZ_C6QsJ5cMjKPyEJYkKesoawinTUHZipoYUopg5RzdVLLLmsg3u_Igz3blm1252i19388rFj3B8N71X2cBfq4AlKBHB1Em48AbGFwsluUQ3AcrXgF3to7_</recordid><startdate>202201</startdate><enddate>202201</enddate><creator>Chen, Xiaoling</creator><creator>Zhang, Man</creator><creator>Xue, Yonghong</creator><creator>Liang, Dahui</creator><creator>An, Wenting</creator><creator>Jia, Gang</creator><creator>Zhao, Hua</creator><creator>Liu, Guangmang</creator><creator>Huang, Zhiqing</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5092-9297</orcidid></search><sort><creationdate>202201</creationdate><title>Effect of dietary L-theanine supplementation on skeletal muscle fiber type transformation in vivo</title><author>Chen, Xiaoling ; Zhang, Man ; Xue, Yonghong ; Liang, Dahui ; An, Wenting ; Jia, Gang ; Zhao, Hua ; Liu, Guangmang ; Huang, Zhiqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-8e00832962048ac6dcf6b6855095c280e6befbea17b4955d24cb08846db759593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>CaN and/or NFATc1 signaling pathway</topic><topic>Dietary Supplements - analysis</topic><topic>Fiber type transition</topic><topic>Gene Expression</topic><topic>Glutamates - metabolism</topic><topic>L-theanine</topic><topic>Male</topic><topic>Mice</topic><topic>Muscle Fibers, Skeletal - metabolism</topic><topic>Muscle Proteins - genetics</topic><topic>Muscle Proteins - metabolism</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Organelle Biogenesis</topic><topic>Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - genetics</topic><topic>Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - metabolism</topic><topic>Sirtuin 1 - genetics</topic><topic>Sirtuin 1 - metabolism</topic><topic>Skeletal muscle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Xiaoling</creatorcontrib><creatorcontrib>Zhang, Man</creatorcontrib><creatorcontrib>Xue, Yonghong</creatorcontrib><creatorcontrib>Liang, Dahui</creatorcontrib><creatorcontrib>An, Wenting</creatorcontrib><creatorcontrib>Jia, Gang</creatorcontrib><creatorcontrib>Zhao, Hua</creatorcontrib><creatorcontrib>Liu, Guangmang</creatorcontrib><creatorcontrib>Huang, Zhiqing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutritional biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Xiaoling</au><au>Zhang, Man</au><au>Xue, Yonghong</au><au>Liang, Dahui</au><au>An, Wenting</au><au>Jia, Gang</au><au>Zhao, Hua</au><au>Liu, Guangmang</au><au>Huang, Zhiqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of dietary L-theanine supplementation on skeletal muscle fiber type transformation in vivo</atitle><jtitle>The Journal of nutritional biochemistry</jtitle><addtitle>J Nutr Biochem</addtitle><date>2022-01</date><risdate>2022</risdate><volume>99</volume><spage>108859</spage><epage>108859</epage><pages>108859-108859</pages><artnum>108859</artnum><issn>0955-2863</issn><eissn>1873-4847</eissn><abstract>The aim of this study was to investigate the effect of dietary L-theanine supplementation on skeletal muscle fiber type transition in mice. Our data indicated that dietary 0.15% L-theanine supplementation significantly increased the mRNA expression levels of muscle fiber type related genes (MyHC I, MyHC IIa, PGC-1α, Sirt1, Tnnt1, Tnnc1, Tnni1, MEF2C) and the protein expression levels of MyHC IIa, myoglobin, PGC-1α, Sirt1 and Troponin I-SS, but significantly decreased the mRNA and protein expression levels of MyHC IIb. Dietary 0.15% L-theanine supplementation significantly increased the activities of SDH and MDH and decreased the activity of LDH. Furthermore, immunofluorescence demonstrated that dietary 0.15% L-theanine supplementation significantly increased the percentage of type I fibers, and significantly decreased the percentage of type II fibers. In addition, we found that dietary 0.15% L-theanine supplementation increased the fatigue-resistant, antioxidant capacity, mitochondrial biogenesis, and function in skeletal muscle of mice. Furthermore, dietary 0.15% L-theanine supplementation significantly increased the mRNA levels of prox1, CaN and NFATc1, the protein levels of prox1, CNA and NFATc1 and the activity of CaN in GAS muscle when compared with the control group. These results indicated that dietary L-theanine supplementation promoted skeletal muscle fiber transition from type II–type I, which might be via activation of CaN and/or NFATc1 signaling pathway.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>34517095</pmid><doi>10.1016/j.jnutbio.2021.108859</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-5092-9297</orcidid></addata></record> |
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| subjects | Animals CaN and/or NFATc1 signaling pathway Dietary Supplements - analysis Fiber type transition Gene Expression Glutamates - metabolism L-theanine Male Mice Muscle Fibers, Skeletal - metabolism Muscle Proteins - genetics Muscle Proteins - metabolism Muscle, Skeletal - metabolism Organelle Biogenesis Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - genetics Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - metabolism Sirtuin 1 - genetics Sirtuin 1 - metabolism Skeletal muscle |
| title | Effect of dietary L-theanine supplementation on skeletal muscle fiber type transformation in vivo |
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