Overview of in‐capillary enzymatic reactions using capillary electrophoresis

This review summarizes the research that has recently been performed on in‐capillary enzymatic reactions integrated with capillary electrophoresis. The manuscript is subdivided in homogeneous and heterogeneous approaches. The main homogeneous techniques are Electrophoretically Mediated Microanalysis...

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Veröffentlicht in:Electrophoresis 2022-01, Vol.43 (1-2), p.57-73
Hauptverfasser: Rozenski, Jef, Asfaw, Adissu Alemayehu, Van Schepdael, Ann
Format: Artikel
Sprache:eng
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Zusammenfassung:This review summarizes the research that has recently been performed on in‐capillary enzymatic reactions integrated with capillary electrophoresis. The manuscript is subdivided in homogeneous and heterogeneous approaches. The main homogeneous techniques are Electrophoretically Mediated Microanalysis, At‐inlet and Transverse Diffusion of Laminar Flow Profiles. The main heterogeneous ones are Immobilized MicroEnzyme Reactors with enzymes grafted on either non‐magnetic or magnetic particles. The overview covers the period from 2018 to early 2021. The applications range from drug discovery over natural products to food, beverage and pesticide analysis.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.202100161