Effect of CD146+ SHED on bone regeneration in a mouse calvaria defect model
Objective Stem cells from human exfoliated deciduous teeth (SHED) have bone regeneration ability and potential therapeutic applications. CD146, a cell adhesion protein expressed by vascular endothelial cells, is involved in osteoblastic differentiation of stem cells. The effect of CD146 on SHED‐medi...
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| Veröffentlicht in: | Oral diseases 2023-03, Vol.29 (2), p.725-734 |
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| creator | Rikitake, Kodai Kunimatsu, Ryo Yoshimi, Yuki Nakajima, Kengo Hiraki, Tomoka Aisyah Rizky Putranti, Nurul Tsuka, Yuji Abe, Takaharu Ando, Kazuyo Hayashi, Yoko Nikawa, Hiroki Tanimoto, Kotaro |
| description | Objective
Stem cells from human exfoliated deciduous teeth (SHED) have bone regeneration ability and potential therapeutic applications. CD146, a cell adhesion protein expressed by vascular endothelial cells, is involved in osteoblastic differentiation of stem cells. The effect of CD146 on SHED‐mediated bone regeneration in vivo remains unknown. We aimed to establish efficient conditions for SHED transplantation.
Materials and methods
SHED were isolated from the pulp of an extracted deciduous tooth and cultured; CD146‐positive (CD146+) and CD146‐negative (CD146−) populations were sorted. Heterogeneous populations of SHED and CD146+ and CD146− cells were transplanted into bone defects generated in the skulls of immunodeficient mice. Micro‐computed tomography was performed immediately and 4 and 8 weeks later. Histological and immunohistochemical assessments were performed 8 weeks later.
Results
Bone regeneration was observed upon transplantation with CD146+ and heterogeneous populations of SHED, with significantly higher bone regeneration observed with CD146+ cells. Bone regeneration was higher in the CD146− group than in the control group, but significantly lower than that in the other transplant groups at 4 and 8 weeks. Histological and immunohistochemical assessments revealed that CD146+ cells promoted bone regeneration and angiogenesis.
Conclusion
Transplantation of CD146+ SHED into bone defects may be useful for bone regeneration. |
| doi_str_mv | 10.1111/odi.14020 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2572230705</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2772168207</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4190-49de47f98ef4a9e8cd9f038a3a3083918e1d8461c0c4a3e9305f348bf9d102743</originalsourceid><addsrcrecordid>eNp1kE1LxDAQhoMofqwe_AMS8KJIdycfbZOj7K4fKHhQwVvIthOptI2mW2X_vXGrHgTnMsPwzMPwEnLIYMxiTXxZjZkEDhtkl2XAElA83YyzSGWScvG0Q_a67gWA5VrwbbIjZMogy9guuZk7h8WSekenMyazM3p_NZ9R39KFb5EGfMYWg11WcVO11NLG9x3SwtbvNlSWlrg-b3yJ9T7Zcrbu8OC7j8jjxfxhepXc3l1eT89vk0IyDYnUJcrcaYVOWo2qKLUDoaywApTQTCErlcxYAYW0ArWA1AmpFk6XDHguxYicDN7X4N967JamqboC69q2GL8zPM05F5BDGtHjP-iL70MbvzM8zznLFIc8UqcDVQTfdQGdeQ1VY8PKMDBfCZuYsFknHNmjb2O_aLD8JX8ijcBkAD6qGlf_m8zd7HpQfgKox4DC</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2772168207</pqid></control><display><type>article</type><title>Effect of CD146+ SHED on bone regeneration in a mouse calvaria defect model</title><source>MEDLINE</source><source>Wiley Journals</source><creator>Rikitake, Kodai ; Kunimatsu, Ryo ; Yoshimi, Yuki ; Nakajima, Kengo ; Hiraki, Tomoka ; Aisyah Rizky Putranti, Nurul ; Tsuka, Yuji ; Abe, Takaharu ; Ando, Kazuyo ; Hayashi, Yoko ; Nikawa, Hiroki ; Tanimoto, Kotaro</creator><creatorcontrib>Rikitake, Kodai ; Kunimatsu, Ryo ; Yoshimi, Yuki ; Nakajima, Kengo ; Hiraki, Tomoka ; Aisyah Rizky Putranti, Nurul ; Tsuka, Yuji ; Abe, Takaharu ; Ando, Kazuyo ; Hayashi, Yoko ; Nikawa, Hiroki ; Tanimoto, Kotaro</creatorcontrib><description>Objective
Stem cells from human exfoliated deciduous teeth (SHED) have bone regeneration ability and potential therapeutic applications. CD146, a cell adhesion protein expressed by vascular endothelial cells, is involved in osteoblastic differentiation of stem cells. The effect of CD146 on SHED‐mediated bone regeneration in vivo remains unknown. We aimed to establish efficient conditions for SHED transplantation.
Materials and methods
SHED were isolated from the pulp of an extracted deciduous tooth and cultured; CD146‐positive (CD146+) and CD146‐negative (CD146−) populations were sorted. Heterogeneous populations of SHED and CD146+ and CD146− cells were transplanted into bone defects generated in the skulls of immunodeficient mice. Micro‐computed tomography was performed immediately and 4 and 8 weeks later. Histological and immunohistochemical assessments were performed 8 weeks later.
Results
Bone regeneration was observed upon transplantation with CD146+ and heterogeneous populations of SHED, with significantly higher bone regeneration observed with CD146+ cells. Bone regeneration was higher in the CD146− group than in the control group, but significantly lower than that in the other transplant groups at 4 and 8 weeks. Histological and immunohistochemical assessments revealed that CD146+ cells promoted bone regeneration and angiogenesis.
Conclusion
Transplantation of CD146+ SHED into bone defects may be useful for bone regeneration.</description><identifier>ISSN: 1354-523X</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.14020</identifier><identifier>PMID: 34510661</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Angiogenesis ; Animals ; Bone growth ; Bone Regeneration ; Calvaria ; CD146 ; CD146 Antigen ; Cell adhesion ; Cell Differentiation ; Computed tomography ; Dental Pulp ; Endothelial Cells ; Humans ; Immunodeficiency ; Mice ; Osteoblastogenesis ; Regeneration ; Skull - surgery ; Stem cell transplantation ; Stem cells ; stem cells from human exfoliated deciduous teeth ; Therapeutic applications ; Tooth, Deciduous ; Transplants & implants ; X-Ray Microtomography</subject><ispartof>Oral diseases, 2023-03, Vol.29 (2), p.725-734</ispartof><rights>2021 Wiley Periodicals LLC</rights><rights>2021 Wiley Periodicals LLC.</rights><rights>2023 Wiley Periodicals LLC</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4190-49de47f98ef4a9e8cd9f038a3a3083918e1d8461c0c4a3e9305f348bf9d102743</citedby><cites>FETCH-LOGICAL-c4190-49de47f98ef4a9e8cd9f038a3a3083918e1d8461c0c4a3e9305f348bf9d102743</cites><orcidid>0000-0002-5016-4957</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fodi.14020$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fodi.14020$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34510661$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rikitake, Kodai</creatorcontrib><creatorcontrib>Kunimatsu, Ryo</creatorcontrib><creatorcontrib>Yoshimi, Yuki</creatorcontrib><creatorcontrib>Nakajima, Kengo</creatorcontrib><creatorcontrib>Hiraki, Tomoka</creatorcontrib><creatorcontrib>Aisyah Rizky Putranti, Nurul</creatorcontrib><creatorcontrib>Tsuka, Yuji</creatorcontrib><creatorcontrib>Abe, Takaharu</creatorcontrib><creatorcontrib>Ando, Kazuyo</creatorcontrib><creatorcontrib>Hayashi, Yoko</creatorcontrib><creatorcontrib>Nikawa, Hiroki</creatorcontrib><creatorcontrib>Tanimoto, Kotaro</creatorcontrib><title>Effect of CD146+ SHED on bone regeneration in a mouse calvaria defect model</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Objective
Stem cells from human exfoliated deciduous teeth (SHED) have bone regeneration ability and potential therapeutic applications. CD146, a cell adhesion protein expressed by vascular endothelial cells, is involved in osteoblastic differentiation of stem cells. The effect of CD146 on SHED‐mediated bone regeneration in vivo remains unknown. We aimed to establish efficient conditions for SHED transplantation.
Materials and methods
SHED were isolated from the pulp of an extracted deciduous tooth and cultured; CD146‐positive (CD146+) and CD146‐negative (CD146−) populations were sorted. Heterogeneous populations of SHED and CD146+ and CD146− cells were transplanted into bone defects generated in the skulls of immunodeficient mice. Micro‐computed tomography was performed immediately and 4 and 8 weeks later. Histological and immunohistochemical assessments were performed 8 weeks later.
Results
Bone regeneration was observed upon transplantation with CD146+ and heterogeneous populations of SHED, with significantly higher bone regeneration observed with CD146+ cells. Bone regeneration was higher in the CD146− group than in the control group, but significantly lower than that in the other transplant groups at 4 and 8 weeks. Histological and immunohistochemical assessments revealed that CD146+ cells promoted bone regeneration and angiogenesis.
Conclusion
Transplantation of CD146+ SHED into bone defects may be useful for bone regeneration.</description><subject>Angiogenesis</subject><subject>Animals</subject><subject>Bone growth</subject><subject>Bone Regeneration</subject><subject>Calvaria</subject><subject>CD146</subject><subject>CD146 Antigen</subject><subject>Cell adhesion</subject><subject>Cell Differentiation</subject><subject>Computed tomography</subject><subject>Dental Pulp</subject><subject>Endothelial Cells</subject><subject>Humans</subject><subject>Immunodeficiency</subject><subject>Mice</subject><subject>Osteoblastogenesis</subject><subject>Regeneration</subject><subject>Skull - surgery</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>stem cells from human exfoliated deciduous teeth</subject><subject>Therapeutic applications</subject><subject>Tooth, Deciduous</subject><subject>Transplants & implants</subject><subject>X-Ray Microtomography</subject><issn>1354-523X</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1LxDAQhoMofqwe_AMS8KJIdycfbZOj7K4fKHhQwVvIthOptI2mW2X_vXGrHgTnMsPwzMPwEnLIYMxiTXxZjZkEDhtkl2XAElA83YyzSGWScvG0Q_a67gWA5VrwbbIjZMogy9guuZk7h8WSekenMyazM3p_NZ9R39KFb5EGfMYWg11WcVO11NLG9x3SwtbvNlSWlrg-b3yJ9T7Zcrbu8OC7j8jjxfxhepXc3l1eT89vk0IyDYnUJcrcaYVOWo2qKLUDoaywApTQTCErlcxYAYW0ArWA1AmpFk6XDHguxYicDN7X4N967JamqboC69q2GL8zPM05F5BDGtHjP-iL70MbvzM8zznLFIc8UqcDVQTfdQGdeQ1VY8PKMDBfCZuYsFknHNmjb2O_aLD8JX8ijcBkAD6qGlf_m8zd7HpQfgKox4DC</recordid><startdate>202303</startdate><enddate>202303</enddate><creator>Rikitake, Kodai</creator><creator>Kunimatsu, Ryo</creator><creator>Yoshimi, Yuki</creator><creator>Nakajima, Kengo</creator><creator>Hiraki, Tomoka</creator><creator>Aisyah Rizky Putranti, Nurul</creator><creator>Tsuka, Yuji</creator><creator>Abe, Takaharu</creator><creator>Ando, Kazuyo</creator><creator>Hayashi, Yoko</creator><creator>Nikawa, Hiroki</creator><creator>Tanimoto, Kotaro</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5016-4957</orcidid></search><sort><creationdate>202303</creationdate><title>Effect of CD146+ SHED on bone regeneration in a mouse calvaria defect model</title><author>Rikitake, Kodai ; Kunimatsu, Ryo ; Yoshimi, Yuki ; Nakajima, Kengo ; Hiraki, Tomoka ; Aisyah Rizky Putranti, Nurul ; Tsuka, Yuji ; Abe, Takaharu ; Ando, Kazuyo ; Hayashi, Yoko ; Nikawa, Hiroki ; Tanimoto, Kotaro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4190-49de47f98ef4a9e8cd9f038a3a3083918e1d8461c0c4a3e9305f348bf9d102743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Angiogenesis</topic><topic>Animals</topic><topic>Bone growth</topic><topic>Bone Regeneration</topic><topic>Calvaria</topic><topic>CD146</topic><topic>CD146 Antigen</topic><topic>Cell adhesion</topic><topic>Cell Differentiation</topic><topic>Computed tomography</topic><topic>Dental Pulp</topic><topic>Endothelial Cells</topic><topic>Humans</topic><topic>Immunodeficiency</topic><topic>Mice</topic><topic>Osteoblastogenesis</topic><topic>Regeneration</topic><topic>Skull - surgery</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>stem cells from human exfoliated deciduous teeth</topic><topic>Therapeutic applications</topic><topic>Tooth, Deciduous</topic><topic>Transplants & implants</topic><topic>X-Ray Microtomography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rikitake, Kodai</creatorcontrib><creatorcontrib>Kunimatsu, Ryo</creatorcontrib><creatorcontrib>Yoshimi, Yuki</creatorcontrib><creatorcontrib>Nakajima, Kengo</creatorcontrib><creatorcontrib>Hiraki, Tomoka</creatorcontrib><creatorcontrib>Aisyah Rizky Putranti, Nurul</creatorcontrib><creatorcontrib>Tsuka, Yuji</creatorcontrib><creatorcontrib>Abe, Takaharu</creatorcontrib><creatorcontrib>Ando, Kazuyo</creatorcontrib><creatorcontrib>Hayashi, Yoko</creatorcontrib><creatorcontrib>Nikawa, Hiroki</creatorcontrib><creatorcontrib>Tanimoto, Kotaro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rikitake, Kodai</au><au>Kunimatsu, Ryo</au><au>Yoshimi, Yuki</au><au>Nakajima, Kengo</au><au>Hiraki, Tomoka</au><au>Aisyah Rizky Putranti, Nurul</au><au>Tsuka, Yuji</au><au>Abe, Takaharu</au><au>Ando, Kazuyo</au><au>Hayashi, Yoko</au><au>Nikawa, Hiroki</au><au>Tanimoto, Kotaro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of CD146+ SHED on bone regeneration in a mouse calvaria defect model</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2023-03</date><risdate>2023</risdate><volume>29</volume><issue>2</issue><spage>725</spage><epage>734</epage><pages>725-734</pages><issn>1354-523X</issn><eissn>1601-0825</eissn><abstract>Objective
Stem cells from human exfoliated deciduous teeth (SHED) have bone regeneration ability and potential therapeutic applications. CD146, a cell adhesion protein expressed by vascular endothelial cells, is involved in osteoblastic differentiation of stem cells. The effect of CD146 on SHED‐mediated bone regeneration in vivo remains unknown. We aimed to establish efficient conditions for SHED transplantation.
Materials and methods
SHED were isolated from the pulp of an extracted deciduous tooth and cultured; CD146‐positive (CD146+) and CD146‐negative (CD146−) populations were sorted. Heterogeneous populations of SHED and CD146+ and CD146− cells were transplanted into bone defects generated in the skulls of immunodeficient mice. Micro‐computed tomography was performed immediately and 4 and 8 weeks later. Histological and immunohistochemical assessments were performed 8 weeks later.
Results
Bone regeneration was observed upon transplantation with CD146+ and heterogeneous populations of SHED, with significantly higher bone regeneration observed with CD146+ cells. Bone regeneration was higher in the CD146− group than in the control group, but significantly lower than that in the other transplant groups at 4 and 8 weeks. Histological and immunohistochemical assessments revealed that CD146+ cells promoted bone regeneration and angiogenesis.
Conclusion
Transplantation of CD146+ SHED into bone defects may be useful for bone regeneration.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>34510661</pmid><doi>10.1111/odi.14020</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-5016-4957</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1354-523X |
| ispartof | Oral diseases, 2023-03, Vol.29 (2), p.725-734 |
| issn | 1354-523X 1601-0825 |
| language | eng |
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| source | MEDLINE; Wiley Journals |
| subjects | Angiogenesis Animals Bone growth Bone Regeneration Calvaria CD146 CD146 Antigen Cell adhesion Cell Differentiation Computed tomography Dental Pulp Endothelial Cells Humans Immunodeficiency Mice Osteoblastogenesis Regeneration Skull - surgery Stem cell transplantation Stem cells stem cells from human exfoliated deciduous teeth Therapeutic applications Tooth, Deciduous Transplants & implants X-Ray Microtomography |
| title | Effect of CD146+ SHED on bone regeneration in a mouse calvaria defect model |
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