Development of a reverse-transcription loop-mediated isothermal amplification assay for the detection of Tobacco mild green mosaic virus (TMGMV)

•An RT-LAMP assay for TMGMV detection was developed and reported for the first time.•The primer set designed for the assay was highly specific for TMGMV in the RT-LAMP assay.•The RT-LAMP assay was more convenient and economical, and 10 times more sensitive than the corresponding RT-PCR assay for TMG...

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Veröffentlicht in:	Journal of virological methods 2021-12, Vol.298, p.114277-114277, Article 114277
Hauptverfasser:	Zhao, Wenhao, Wu, Shuhua, Du, Linlin, Li, Tingfang, Cheng, Zhaobang, Zhou, Yijun, Ji, Yinghua
Format:	Artikel
Sprache:	eng
Schlagworte:	Capsicum annuum Molecular Diagnostic Techniques Nicotiana - genetics Nucleic Acid Amplification Techniques - methods Pepper Plant Diseases Reverse-transcription loop-mediated isothermal amplification (RT-LAMP) Sensitivity and Specificity Tobacco mild green mosaic virus (TMGMV) Tobamovirus - genetics Virus detection
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