A DNA Molecular Robot that Autonomously Walks on the Cell Membrane to Drive Cell Motility
Synthetic molecular robots can execute sophisticated molecular tasks at nanometer resolution. However, a molecular robot capable of controlling cellular behavior remains unexplored. Herein, we report a self‐propelled DNA robot operating on the cell membrane to control the migration of a cell. Driven...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2021-12, Vol.60 (50), p.26087-26095 |
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| creator | Li, Hao Gao, Jing Cao, Lei Xie, Xuan Fan, Jiahui Wang, Hongda Wang, Hong‐Hui Nie, Zhou |
| description | Synthetic molecular robots can execute sophisticated molecular tasks at nanometer resolution. However, a molecular robot capable of controlling cellular behavior remains unexplored. Herein, we report a self‐propelled DNA robot operating on the cell membrane to control the migration of a cell. Driven by DNAzyme catalytic activity, the DNA robot could autonomously and stepwise move on the membrane‐floating cell‐surface receptors in a stochastic manner and simultaneously trigger the receptor‐dimerization to activate downstream signaling for cell motility. The cell membrane‐associated continuous motion and operation of a DNA robot allowed for the ultrasensitive regulation of MET/AKT signaling and cytoskeleton remodeling to enhance cell migration. Finally, we designed distinct conditional DNA robots to orthogonally manipulate the cell migration in a coculture of mixed cell populations. We have developed a novel strategy to engineer a cell‐driving molecular robot, representing a promising avenue for precise cell manipulation with nanoscale resolution.
A DNA molecular robot that autonomously walks on the cell membrane to drive the cell motility has been developed. The DNA robot could move stepwise on the membrane‐floating cell‐surface receptors in a stochastic manner and simultaneously trigger the receptor‐dimerization to activate downstream signal pathway regulation of desired cellular behavior. |
| doi_str_mv | 10.1002/anie.202108210 |
| format | Article |
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A DNA molecular robot that autonomously walks on the cell membrane to drive the cell motility has been developed. The DNA robot could move stepwise on the membrane‐floating cell‐surface receptors in a stochastic manner and simultaneously trigger the receptor‐dimerization to activate downstream signal pathway regulation of desired cellular behavior.</description><edition>International ed. in English</edition><identifier>ISSN: 1433-7851</identifier><identifier>EISSN: 1521-3773</identifier><identifier>DOI: 10.1002/anie.202108210</identifier><identifier>PMID: 34490693</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>A549 Cells ; AKT protein ; Catalytic activity ; Cell adhesion & migration ; cell manipulation ; Cell Membrane - chemistry ; Cell Membrane - metabolism ; Cell membranes ; Cell migration ; Cell Movement ; Cell surface ; Cytoskeleton ; Deoxyribonucleic acid ; Dimerization ; DNA ; DNA - chemistry ; DNA - metabolism ; DNA robots ; DNA walkers ; DNA, Catalytic - metabolism ; DNAzymes ; Humans ; membrane receptors ; Membranes ; Motility ; Receptors ; Robot dynamics ; Robots ; Signaling ; Stochasticity</subject><ispartof>Angewandte Chemie International Edition, 2021-12, Vol.60 (50), p.26087-26095</ispartof><rights>2021 Wiley‐VCH GmbH</rights><rights>2021 Wiley-VCH GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3730-a5f1cf989a9b8ac23c4075221a5ecbcd977801037c903e9d2462b486d8144bf33</citedby><cites>FETCH-LOGICAL-c3730-a5f1cf989a9b8ac23c4075221a5ecbcd977801037c903e9d2462b486d8144bf33</cites><orcidid>0000-0001-9864-2965</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fanie.202108210$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fanie.202108210$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34490693$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Hao</creatorcontrib><creatorcontrib>Gao, Jing</creatorcontrib><creatorcontrib>Cao, Lei</creatorcontrib><creatorcontrib>Xie, Xuan</creatorcontrib><creatorcontrib>Fan, Jiahui</creatorcontrib><creatorcontrib>Wang, Hongda</creatorcontrib><creatorcontrib>Wang, Hong‐Hui</creatorcontrib><creatorcontrib>Nie, Zhou</creatorcontrib><title>A DNA Molecular Robot that Autonomously Walks on the Cell Membrane to Drive Cell Motility</title><title>Angewandte Chemie International Edition</title><addtitle>Angew Chem Int Ed Engl</addtitle><description>Synthetic molecular robots can execute sophisticated molecular tasks at nanometer resolution. However, a molecular robot capable of controlling cellular behavior remains unexplored. Herein, we report a self‐propelled DNA robot operating on the cell membrane to control the migration of a cell. Driven by DNAzyme catalytic activity, the DNA robot could autonomously and stepwise move on the membrane‐floating cell‐surface receptors in a stochastic manner and simultaneously trigger the receptor‐dimerization to activate downstream signaling for cell motility. The cell membrane‐associated continuous motion and operation of a DNA robot allowed for the ultrasensitive regulation of MET/AKT signaling and cytoskeleton remodeling to enhance cell migration. Finally, we designed distinct conditional DNA robots to orthogonally manipulate the cell migration in a coculture of mixed cell populations. We have developed a novel strategy to engineer a cell‐driving molecular robot, representing a promising avenue for precise cell manipulation with nanoscale resolution.
A DNA molecular robot that autonomously walks on the cell membrane to drive the cell motility has been developed. The DNA robot could move stepwise on the membrane‐floating cell‐surface receptors in a stochastic manner and simultaneously trigger the receptor‐dimerization to activate downstream signal pathway regulation of desired cellular behavior.</description><subject>A549 Cells</subject><subject>AKT protein</subject><subject>Catalytic activity</subject><subject>Cell adhesion & migration</subject><subject>cell manipulation</subject><subject>Cell Membrane - chemistry</subject><subject>Cell Membrane - metabolism</subject><subject>Cell membranes</subject><subject>Cell migration</subject><subject>Cell Movement</subject><subject>Cell surface</subject><subject>Cytoskeleton</subject><subject>Deoxyribonucleic acid</subject><subject>Dimerization</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>DNA - metabolism</subject><subject>DNA robots</subject><subject>DNA walkers</subject><subject>DNA, Catalytic - metabolism</subject><subject>DNAzymes</subject><subject>Humans</subject><subject>membrane receptors</subject><subject>Membranes</subject><subject>Motility</subject><subject>Receptors</subject><subject>Robot dynamics</subject><subject>Robots</subject><subject>Signaling</subject><subject>Stochasticity</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtLxDAQxoMorq-rRwl48dI1rzbJseyuD_ABooinkGZTrKaNJq2y_71ZVlfw4mGYYeY3Hx8fAIcYjTFC5FR3jR0TRDASqTbADs4JzijndDPNjNKMixyPwG6ML4kXAhXbYEQZk6iQdAc8lXB6U8Jr76wZnA7wzle-h_2z7mE59L7zrR-iW8BH7V4j9F06WTixzsFr21ZBdxb2Hk5D8_Gz9n3jmn6xD7Zq7aI9-O574OFsdj-5yK5uzy8n5VVmKKco03mNTS2F1LIS2hBqGOI5IVjn1lRmLjkXCCPKjUTUyjlhBamYKOYCM1bVlO6Bk5XuW_Dvg429aptokpNkLVlXJOcIY0x5ntDjP-iLH0KX3ClSICplLgqWqPGKMsHHGGyt3kLT6rBQGKll6GoZulqHnh6OvmWHqrXzNf6TcgLkCvhsnF38I6fKm8vZr_gXxRiLdA</recordid><startdate>20211206</startdate><enddate>20211206</enddate><creator>Li, Hao</creator><creator>Gao, Jing</creator><creator>Cao, Lei</creator><creator>Xie, Xuan</creator><creator>Fan, Jiahui</creator><creator>Wang, Hongda</creator><creator>Wang, Hong‐Hui</creator><creator>Nie, Zhou</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9864-2965</orcidid></search><sort><creationdate>20211206</creationdate><title>A DNA Molecular Robot that Autonomously Walks on the Cell Membrane to Drive Cell Motility</title><author>Li, Hao ; Gao, Jing ; Cao, Lei ; Xie, Xuan ; Fan, Jiahui ; Wang, Hongda ; Wang, Hong‐Hui ; Nie, Zhou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3730-a5f1cf989a9b8ac23c4075221a5ecbcd977801037c903e9d2462b486d8144bf33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>A549 Cells</topic><topic>AKT protein</topic><topic>Catalytic activity</topic><topic>Cell adhesion & migration</topic><topic>cell manipulation</topic><topic>Cell Membrane - chemistry</topic><topic>Cell Membrane - metabolism</topic><topic>Cell membranes</topic><topic>Cell migration</topic><topic>Cell Movement</topic><topic>Cell surface</topic><topic>Cytoskeleton</topic><topic>Deoxyribonucleic acid</topic><topic>Dimerization</topic><topic>DNA</topic><topic>DNA - chemistry</topic><topic>DNA - metabolism</topic><topic>DNA robots</topic><topic>DNA walkers</topic><topic>DNA, Catalytic - metabolism</topic><topic>DNAzymes</topic><topic>Humans</topic><topic>membrane receptors</topic><topic>Membranes</topic><topic>Motility</topic><topic>Receptors</topic><topic>Robot dynamics</topic><topic>Robots</topic><topic>Signaling</topic><topic>Stochasticity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Hao</creatorcontrib><creatorcontrib>Gao, Jing</creatorcontrib><creatorcontrib>Cao, Lei</creatorcontrib><creatorcontrib>Xie, Xuan</creatorcontrib><creatorcontrib>Fan, Jiahui</creatorcontrib><creatorcontrib>Wang, Hongda</creatorcontrib><creatorcontrib>Wang, Hong‐Hui</creatorcontrib><creatorcontrib>Nie, Zhou</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Angewandte Chemie International Edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Hao</au><au>Gao, Jing</au><au>Cao, Lei</au><au>Xie, Xuan</au><au>Fan, Jiahui</au><au>Wang, Hongda</au><au>Wang, Hong‐Hui</au><au>Nie, Zhou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A DNA Molecular Robot that Autonomously Walks on the Cell Membrane to Drive Cell Motility</atitle><jtitle>Angewandte Chemie International Edition</jtitle><addtitle>Angew Chem Int Ed Engl</addtitle><date>2021-12-06</date><risdate>2021</risdate><volume>60</volume><issue>50</issue><spage>26087</spage><epage>26095</epage><pages>26087-26095</pages><issn>1433-7851</issn><eissn>1521-3773</eissn><abstract>Synthetic molecular robots can execute sophisticated molecular tasks at nanometer resolution. However, a molecular robot capable of controlling cellular behavior remains unexplored. Herein, we report a self‐propelled DNA robot operating on the cell membrane to control the migration of a cell. Driven by DNAzyme catalytic activity, the DNA robot could autonomously and stepwise move on the membrane‐floating cell‐surface receptors in a stochastic manner and simultaneously trigger the receptor‐dimerization to activate downstream signaling for cell motility. The cell membrane‐associated continuous motion and operation of a DNA robot allowed for the ultrasensitive regulation of MET/AKT signaling and cytoskeleton remodeling to enhance cell migration. Finally, we designed distinct conditional DNA robots to orthogonally manipulate the cell migration in a coculture of mixed cell populations. We have developed a novel strategy to engineer a cell‐driving molecular robot, representing a promising avenue for precise cell manipulation with nanoscale resolution.
A DNA molecular robot that autonomously walks on the cell membrane to drive the cell motility has been developed. The DNA robot could move stepwise on the membrane‐floating cell‐surface receptors in a stochastic manner and simultaneously trigger the receptor‐dimerization to activate downstream signal pathway regulation of desired cellular behavior.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>34490693</pmid><doi>10.1002/anie.202108210</doi><tpages>9</tpages><edition>International ed. in English</edition><orcidid>https://orcid.org/0000-0001-9864-2965</orcidid></addata></record> |
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| subjects | A549 Cells AKT protein Catalytic activity Cell adhesion & migration cell manipulation Cell Membrane - chemistry Cell Membrane - metabolism Cell membranes Cell migration Cell Movement Cell surface Cytoskeleton Deoxyribonucleic acid Dimerization DNA DNA - chemistry DNA - metabolism DNA robots DNA walkers DNA, Catalytic - metabolism DNAzymes Humans membrane receptors Membranes Motility Receptors Robot dynamics Robots Signaling Stochasticity |
| title | A DNA Molecular Robot that Autonomously Walks on the Cell Membrane to Drive Cell Motility |
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