Improving the alkaline stability of pepsin through rational protein design using renin, an alkaline-stable aspartic protease, as a structural and functional reference

•The individual contributions of Asp60 and Asp159 may contribute to the alkaline inactivity of pepsin.•Flexible loop regions associated with Glu4 and His53 act as a potential initiator to the alkaline denaturation of pepsin.•Reducing electrostatic repulsion and stabilizing β-structures is insufficie...

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Veröffentlicht in:	Enzyme and microbial technology 2021-10, Vol.150, p.109871-109871, Article 109871
Hauptverfasser:	Grahame, Douglas A.S., Dupuis, John H., Bryksa, Brian C., Tanaka, Takuji, Yada, Rickey Y.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aspartic protease Pepsin pH stability Rational re-design Renin Structure-Function
Online-Zugang:	Volltext
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