Streptomyces endocoffeicus sp. nov., an endophytic actinomycete isolated from Coffea arabica (L.)
An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110 T , was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110 T was a member of the genus Streptomyces...
Gespeichert in:
| Veröffentlicht in: | Antonie van Leeuwenhoek 2021-11, Vol.114 (11), p.1889-1898 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1898 |
|---|---|
| container_issue | 11 |
| container_start_page | 1889 |
| container_title | Antonie van Leeuwenhoek |
| container_volume | 114 |
| creator | Nammali, Achararak Intaraudom, Chakapong Pittayakhajonwut, Pattama Suriyachadkun, Chanwit Tadtong, Sarin Tanasupawat, Somboon Thawai, Chitti |
| description | An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110
T
, was isolated from the surface-sterilised root of
Coffea arabica
L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110
T
was a member of the genus
Streptomyces
and showed the closest similarities to
Streptomyces buecherae
AC541
T
(99.2%), followed by
Streptomyces rapamycinicus
NRRL B-5491
T
(99.1%),
Streptomyces luteoverticillatus
NBRC 3840
T
(99.1%),
Streptomyces coerulescens
NBRC 12758
T
(99.1%), and
Streptomyces iranensis
HM 35
T
(99.0%). Strain CA3R110
T
contained
LL
-diaminopimelic acid in cell peptidoglycan, MK-9(H
6
), and MK-9(H
8
) as major menaquinone,
iso
-C
16:0,
iso
-C
15:0,
C
16:0
as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus
Streptomyces
. A low digital DNA–DNA hybridization ( |
| doi_str_mv | 10.1007/s10482-021-01648-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2569374117</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2583218490</sourcerecordid><originalsourceid>FETCH-LOGICAL-c352t-a3dc93deb7959aab12f701524c44a0aa355a09ae4ceb9d5bb1a8f79f466f63f83</originalsourceid><addsrcrecordid>eNp9kE1LxDAQhoMouK7-AU8BLwp2zVeb5iiLX7DgQT2HaZpol7apSSu7_97uVhA8eBqGeZ6X4UXonJIFJUTeREpEzhLCaEJoJvJkc4BmNJUsUZlSh2hGCOFJRiQ7RicxrsdVZbmcIXjpg-1632yNjdi2pTfeOVuZIeLYLXDrvxbXGNr9qfvY9pXBYPqq3Ru9xVX0NfS2xC74Bi93MmAIUFQG8OVqcXWKjhzU0Z79zDl6u797XT4mq-eHp-XtKjE8ZX0CvDSKl7aQKlUABWVOEpoyYYQAAsDTFIgCK4wtVJkWBYXcSeVElrmMu5zP0eWU2wX_OdjY66aKxtY1tNYPUbM0U1wKSuWIXvxB134I7fjdSOWc0VwoMlJsokzwMQbrdBeqBsJWU6J3reupdT22rvet680o8UmKI9y-2_Ab_Y_1DWowhbo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2583218490</pqid></control><display><type>article</type><title>Streptomyces endocoffeicus sp. nov., an endophytic actinomycete isolated from Coffea arabica (L.)</title><source>SpringerLink Journals - AutoHoldings</source><creator>Nammali, Achararak ; Intaraudom, Chakapong ; Pittayakhajonwut, Pattama ; Suriyachadkun, Chanwit ; Tadtong, Sarin ; Tanasupawat, Somboon ; Thawai, Chitti</creator><creatorcontrib>Nammali, Achararak ; Intaraudom, Chakapong ; Pittayakhajonwut, Pattama ; Suriyachadkun, Chanwit ; Tadtong, Sarin ; Tanasupawat, Somboon ; Thawai, Chitti</creatorcontrib><description>An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110
T
, was isolated from the surface-sterilised root of
Coffea arabica
L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110
T
was a member of the genus
Streptomyces
and showed the closest similarities to
Streptomyces buecherae
AC541
T
(99.2%), followed by
Streptomyces rapamycinicus
NRRL B-5491
T
(99.1%),
Streptomyces luteoverticillatus
NBRC 3840
T
(99.1%),
Streptomyces coerulescens
NBRC 12758
T
(99.1%), and
Streptomyces iranensis
HM 35
T
(99.0%). Strain CA3R110
T
contained
LL
-diaminopimelic acid in cell peptidoglycan, MK-9(H
6
), and MK-9(H
8
) as major menaquinone,
iso
-C
16:0,
iso
-C
15:0,
C
16:0
as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus
Streptomyces
. A low digital DNA–DNA hybridization (< 55.7%) and average nucleotide identity-blast (ANIb) (< 92.2%) values revealed that strain CA3R110
T
could be distinguished from any known
Streptomyces
species. With the differences in phenotypic and genotypic data, strain CA3R110
T
represents a novel species of genus
Streptomyces
, for which the name
Streptomyces endocoffeicus
sp. nov. is proposed. The type strain is CA3R110
T
(= TBRC 11245
T
= NBRC 114296
T
).</description><identifier>ISSN: 0003-6072</identifier><identifier>EISSN: 1572-9699</identifier><identifier>DOI: 10.1007/s10482-021-01648-x</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Biomedical and Life Sciences ; Cardiolipin ; Chromatography ; Coffea arabica ; Deoxyribonucleic acid ; DNA ; Endophytes ; Fatty acids ; Genetic engineering ; Genomes ; Hybridization ; Life Sciences ; Medical Microbiology ; Menaquinones ; Metabolites ; Microbiology ; Microorganisms ; Morphology ; New species ; Nucleotides ; Original Paper ; Pathogens ; Peptidoglycans ; Phosphatidylethanolamine ; Phosphatidylglycerol ; Phosphatidylinositol ; Plant Sciences ; rRNA 16S ; Science ; Sequence analysis ; Soil Science & Conservation ; Streptomyces</subject><ispartof>Antonie van Leeuwenhoek, 2021-11, Vol.114 (11), p.1889-1898</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-a3dc93deb7959aab12f701524c44a0aa355a09ae4ceb9d5bb1a8f79f466f63f83</citedby><cites>FETCH-LOGICAL-c352t-a3dc93deb7959aab12f701524c44a0aa355a09ae4ceb9d5bb1a8f79f466f63f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10482-021-01648-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10482-021-01648-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Nammali, Achararak</creatorcontrib><creatorcontrib>Intaraudom, Chakapong</creatorcontrib><creatorcontrib>Pittayakhajonwut, Pattama</creatorcontrib><creatorcontrib>Suriyachadkun, Chanwit</creatorcontrib><creatorcontrib>Tadtong, Sarin</creatorcontrib><creatorcontrib>Tanasupawat, Somboon</creatorcontrib><creatorcontrib>Thawai, Chitti</creatorcontrib><title>Streptomyces endocoffeicus sp. nov., an endophytic actinomycete isolated from Coffea arabica (L.)</title><title>Antonie van Leeuwenhoek</title><addtitle>Antonie van Leeuwenhoek</addtitle><description>An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110
T
, was isolated from the surface-sterilised root of
Coffea arabica
L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110
T
was a member of the genus
Streptomyces
and showed the closest similarities to
Streptomyces buecherae
AC541
T
(99.2%), followed by
Streptomyces rapamycinicus
NRRL B-5491
T
(99.1%),
Streptomyces luteoverticillatus
NBRC 3840
T
(99.1%),
Streptomyces coerulescens
NBRC 12758
T
(99.1%), and
Streptomyces iranensis
HM 35
T
(99.0%). Strain CA3R110
T
contained
LL
-diaminopimelic acid in cell peptidoglycan, MK-9(H
6
), and MK-9(H
8
) as major menaquinone,
iso
-C
16:0,
iso
-C
15:0,
C
16:0
as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus
Streptomyces
. A low digital DNA–DNA hybridization (< 55.7%) and average nucleotide identity-blast (ANIb) (< 92.2%) values revealed that strain CA3R110
T
could be distinguished from any known
Streptomyces
species. With the differences in phenotypic and genotypic data, strain CA3R110
T
represents a novel species of genus
Streptomyces
, for which the name
Streptomyces endocoffeicus
sp. nov. is proposed. The type strain is CA3R110
T
(= TBRC 11245
T
= NBRC 114296
T
).</description><subject>Biomedical and Life Sciences</subject><subject>Cardiolipin</subject><subject>Chromatography</subject><subject>Coffea arabica</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Endophytes</subject><subject>Fatty acids</subject><subject>Genetic engineering</subject><subject>Genomes</subject><subject>Hybridization</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Menaquinones</subject><subject>Metabolites</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Morphology</subject><subject>New species</subject><subject>Nucleotides</subject><subject>Original Paper</subject><subject>Pathogens</subject><subject>Peptidoglycans</subject><subject>Phosphatidylethanolamine</subject><subject>Phosphatidylglycerol</subject><subject>Phosphatidylinositol</subject><subject>Plant Sciences</subject><subject>rRNA 16S</subject><subject>Science</subject><subject>Sequence analysis</subject><subject>Soil Science & Conservation</subject><subject>Streptomyces</subject><issn>0003-6072</issn><issn>1572-9699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kE1LxDAQhoMouK7-AU8BLwp2zVeb5iiLX7DgQT2HaZpol7apSSu7_97uVhA8eBqGeZ6X4UXonJIFJUTeREpEzhLCaEJoJvJkc4BmNJUsUZlSh2hGCOFJRiQ7RicxrsdVZbmcIXjpg-1632yNjdi2pTfeOVuZIeLYLXDrvxbXGNr9qfvY9pXBYPqq3Ru9xVX0NfS2xC74Bi93MmAIUFQG8OVqcXWKjhzU0Z79zDl6u797XT4mq-eHp-XtKjE8ZX0CvDSKl7aQKlUABWVOEpoyYYQAAsDTFIgCK4wtVJkWBYXcSeVElrmMu5zP0eWU2wX_OdjY66aKxtY1tNYPUbM0U1wKSuWIXvxB134I7fjdSOWc0VwoMlJsokzwMQbrdBeqBsJWU6J3reupdT22rvet680o8UmKI9y-2_Ab_Y_1DWowhbo</recordid><startdate>20211101</startdate><enddate>20211101</enddate><creator>Nammali, Achararak</creator><creator>Intaraudom, Chakapong</creator><creator>Pittayakhajonwut, Pattama</creator><creator>Suriyachadkun, Chanwit</creator><creator>Tadtong, Sarin</creator><creator>Tanasupawat, Somboon</creator><creator>Thawai, Chitti</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20211101</creationdate><title>Streptomyces endocoffeicus sp. nov., an endophytic actinomycete isolated from Coffea arabica (L.)</title><author>Nammali, Achararak ; Intaraudom, Chakapong ; Pittayakhajonwut, Pattama ; Suriyachadkun, Chanwit ; Tadtong, Sarin ; Tanasupawat, Somboon ; Thawai, Chitti</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-a3dc93deb7959aab12f701524c44a0aa355a09ae4ceb9d5bb1a8f79f466f63f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biomedical and Life Sciences</topic><topic>Cardiolipin</topic><topic>Chromatography</topic><topic>Coffea arabica</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Endophytes</topic><topic>Fatty acids</topic><topic>Genetic engineering</topic><topic>Genomes</topic><topic>Hybridization</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Menaquinones</topic><topic>Metabolites</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Morphology</topic><topic>New species</topic><topic>Nucleotides</topic><topic>Original Paper</topic><topic>Pathogens</topic><topic>Peptidoglycans</topic><topic>Phosphatidylethanolamine</topic><topic>Phosphatidylglycerol</topic><topic>Phosphatidylinositol</topic><topic>Plant Sciences</topic><topic>rRNA 16S</topic><topic>Science</topic><topic>Sequence analysis</topic><topic>Soil Science & Conservation</topic><topic>Streptomyces</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nammali, Achararak</creatorcontrib><creatorcontrib>Intaraudom, Chakapong</creatorcontrib><creatorcontrib>Pittayakhajonwut, Pattama</creatorcontrib><creatorcontrib>Suriyachadkun, Chanwit</creatorcontrib><creatorcontrib>Tadtong, Sarin</creatorcontrib><creatorcontrib>Tanasupawat, Somboon</creatorcontrib><creatorcontrib>Thawai, Chitti</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Antonie van Leeuwenhoek</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nammali, Achararak</au><au>Intaraudom, Chakapong</au><au>Pittayakhajonwut, Pattama</au><au>Suriyachadkun, Chanwit</au><au>Tadtong, Sarin</au><au>Tanasupawat, Somboon</au><au>Thawai, Chitti</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Streptomyces endocoffeicus sp. nov., an endophytic actinomycete isolated from Coffea arabica (L.)</atitle><jtitle>Antonie van Leeuwenhoek</jtitle><stitle>Antonie van Leeuwenhoek</stitle><date>2021-11-01</date><risdate>2021</risdate><volume>114</volume><issue>11</issue><spage>1889</spage><epage>1898</epage><pages>1889-1898</pages><issn>0003-6072</issn><eissn>1572-9699</eissn><abstract>An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110
T
, was isolated from the surface-sterilised root of
Coffea arabica
L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110
T
was a member of the genus
Streptomyces
and showed the closest similarities to
Streptomyces buecherae
AC541
T
(99.2%), followed by
Streptomyces rapamycinicus
NRRL B-5491
T
(99.1%),
Streptomyces luteoverticillatus
NBRC 3840
T
(99.1%),
Streptomyces coerulescens
NBRC 12758
T
(99.1%), and
Streptomyces iranensis
HM 35
T
(99.0%). Strain CA3R110
T
contained
LL
-diaminopimelic acid in cell peptidoglycan, MK-9(H
6
), and MK-9(H
8
) as major menaquinone,
iso
-C
16:0,
iso
-C
15:0,
C
16:0
as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus
Streptomyces
. A low digital DNA–DNA hybridization (< 55.7%) and average nucleotide identity-blast (ANIb) (< 92.2%) values revealed that strain CA3R110
T
could be distinguished from any known
Streptomyces
species. With the differences in phenotypic and genotypic data, strain CA3R110
T
represents a novel species of genus
Streptomyces
, for which the name
Streptomyces endocoffeicus
sp. nov. is proposed. The type strain is CA3R110
T
(= TBRC 11245
T
= NBRC 114296
T
).</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s10482-021-01648-x</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-6072 |
| ispartof | Antonie van Leeuwenhoek, 2021-11, Vol.114 (11), p.1889-1898 |
| issn | 0003-6072 1572-9699 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2569374117 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Biomedical and Life Sciences Cardiolipin Chromatography Coffea arabica Deoxyribonucleic acid DNA Endophytes Fatty acids Genetic engineering Genomes Hybridization Life Sciences Medical Microbiology Menaquinones Metabolites Microbiology Microorganisms Morphology New species Nucleotides Original Paper Pathogens Peptidoglycans Phosphatidylethanolamine Phosphatidylglycerol Phosphatidylinositol Plant Sciences rRNA 16S Science Sequence analysis Soil Science & Conservation Streptomyces |
| title | Streptomyces endocoffeicus sp. nov., an endophytic actinomycete isolated from Coffea arabica (L.) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T19%3A18%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Streptomyces%20endocoffeicus%20sp.%20nov.,%20an%20endophytic%20actinomycete%20isolated%20from%20Coffea%20arabica%20(L.)&rft.jtitle=Antonie%20van%20Leeuwenhoek&rft.au=Nammali,%20Achararak&rft.date=2021-11-01&rft.volume=114&rft.issue=11&rft.spage=1889&rft.epage=1898&rft.pages=1889-1898&rft.issn=0003-6072&rft.eissn=1572-9699&rft_id=info:doi/10.1007/s10482-021-01648-x&rft_dat=%3Cproquest_cross%3E2583218490%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2583218490&rft_id=info:pmid/&rfr_iscdi=true |