Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation
Mucins are the major proteins that distributed on the intestinal mucosa layer and protect the intestine from pathogens infection. The composition of intestinal mucin O -glycans can affect the health of the gastrointestinal tract in pigs. Porcine intestinal mucosa is widely used as the main raw mater...
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| Veröffentlicht in: | Glycoconjugate journal 2021-10, Vol.38 (5), p.527-537 |
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| creator | Zhang, Xin Wang, Chen Han, Qing Chen, Xuan Li, Guoyun Yu, Guangli |
| description | Mucins are the major proteins that distributed on the intestinal mucosa layer and protect the intestine from pathogens infection. The composition of intestinal mucin
O
-glycans can affect the health of the gastrointestinal tract in pigs. Porcine intestinal mucosa is widely used as the main raw material of heparin extraction. The heparin extraction residues rich in mucins were usually wasted. The structure of mucin derived
O
-glycans in porcine intestinal mucosa are currently unknown. In this study, we isolated the mucins from the heparin extraction residues and profiled the
O
-glycans. After heparin extraction, mucin was digested with trypsin, and separated by strong anion exchange chromatography. The mucin derived
O
-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS). Thirty five kinds of
O
-glycans were identified, most of which were Core 3-derived glycans. In particular, the
O
-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total
O
-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon. The high content sialylated mucin may explain its effect in biological processes. Furthermore, the immunological activity results indicated that the porcine intestinal mucin could promote phagocytosis and proliferation without any cytotoxic effects, which may aid in the development of immunomodulators. |
| doi_str_mv | 10.1007/s10719-021-10014-y |
| format | Article |
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O
-glycans can affect the health of the gastrointestinal tract in pigs. Porcine intestinal mucosa is widely used as the main raw material of heparin extraction. The heparin extraction residues rich in mucins were usually wasted. The structure of mucin derived
O
-glycans in porcine intestinal mucosa are currently unknown. In this study, we isolated the mucins from the heparin extraction residues and profiled the
O
-glycans. After heparin extraction, mucin was digested with trypsin, and separated by strong anion exchange chromatography. The mucin derived
O
-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS). Thirty five kinds of
O
-glycans were identified, most of which were Core 3-derived glycans. In particular, the
O
-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total
O
-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon. The high content sialylated mucin may explain its effect in biological processes. Furthermore, the immunological activity results indicated that the porcine intestinal mucin could promote phagocytosis and proliferation without any cytotoxic effects, which may aid in the development of immunomodulators.</description><identifier>ISSN: 0282-0080</identifier><identifier>EISSN: 1573-4986</identifier><identifier>DOI: 10.1007/s10719-021-10014-y</identifier><identifier>PMID: 34480673</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Antibiotics ; Anticoagulants ; Biochemistry ; Biomedical and Life Sciences ; Chromatography ; Cytotoxicity ; Fourier transforms ; Gastrointestinal tract ; Glycopeptides ; Heparin ; Heparin - chemistry ; Heparin - metabolism ; High-performance liquid chromatography ; Immunology ; Immunomodulation ; Intestinal Mucosa - chemistry ; Intestinal Mucosa - metabolism ; Intestine ; Life Sciences ; Macrophages - drug effects ; Macrophages - immunology ; Mass spectroscopy ; Mice ; Mucin ; Mucins - chemistry ; Mucins - pharmacology ; Mucosa ; Original Article ; Pathology ; Phagocytosis ; Polysaccharides ; RAW 264.7 Cells ; Swine ; Trypsin</subject><ispartof>Glycoconjugate journal, 2021-10, Vol.38 (5), p.527-537</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-a45041dd88c0c5fe59710667ee8f7a143773e24b7107d087d95ae2e99d629b3c3</citedby><cites>FETCH-LOGICAL-c375t-a45041dd88c0c5fe59710667ee8f7a143773e24b7107d087d95ae2e99d629b3c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10719-021-10014-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10719-021-10014-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34480673$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Xin</creatorcontrib><creatorcontrib>Wang, Chen</creatorcontrib><creatorcontrib>Han, Qing</creatorcontrib><creatorcontrib>Chen, Xuan</creatorcontrib><creatorcontrib>Li, Guoyun</creatorcontrib><creatorcontrib>Yu, Guangli</creatorcontrib><title>Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation</title><title>Glycoconjugate journal</title><addtitle>Glycoconj J</addtitle><addtitle>Glycoconj J</addtitle><description>Mucins are the major proteins that distributed on the intestinal mucosa layer and protect the intestine from pathogens infection. The composition of intestinal mucin
O
-glycans can affect the health of the gastrointestinal tract in pigs. Porcine intestinal mucosa is widely used as the main raw material of heparin extraction. The heparin extraction residues rich in mucins were usually wasted. The structure of mucin derived
O
-glycans in porcine intestinal mucosa are currently unknown. In this study, we isolated the mucins from the heparin extraction residues and profiled the
O
-glycans. After heparin extraction, mucin was digested with trypsin, and separated by strong anion exchange chromatography. The mucin derived
O
-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS). Thirty five kinds of
O
-glycans were identified, most of which were Core 3-derived glycans. In particular, the
O
-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total
O
-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon. The high content sialylated mucin may explain its effect in biological processes. Furthermore, the immunological activity results indicated that the porcine intestinal mucin could promote phagocytosis and proliferation without any cytotoxic effects, which may aid in the development of immunomodulators.</description><subject>Animals</subject><subject>Antibiotics</subject><subject>Anticoagulants</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Chromatography</subject><subject>Cytotoxicity</subject><subject>Fourier transforms</subject><subject>Gastrointestinal tract</subject><subject>Glycopeptides</subject><subject>Heparin</subject><subject>Heparin - chemistry</subject><subject>Heparin - metabolism</subject><subject>High-performance liquid chromatography</subject><subject>Immunology</subject><subject>Immunomodulation</subject><subject>Intestinal Mucosa - chemistry</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestine</subject><subject>Life Sciences</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - immunology</subject><subject>Mass spectroscopy</subject><subject>Mice</subject><subject>Mucin</subject><subject>Mucins - chemistry</subject><subject>Mucins - pharmacology</subject><subject>Mucosa</subject><subject>Original Article</subject><subject>Pathology</subject><subject>Phagocytosis</subject><subject>Polysaccharides</subject><subject>RAW 264.7 Cells</subject><subject>Swine</subject><subject>Trypsin</subject><issn>0282-0080</issn><issn>1573-4986</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1u1TAQhS0EopfCC7BAltiwMfgvccwOVUCRKnUD68jXmaSuHDvYTtU8Cy-Lwy0gsWBljec7Z-w5CL1k9C2jVL3LjCqmCeWM1JpJsj1CB9YoQaTu2sfoQHnHCaUdPUPPcr6lVSR59xSdCSk72ipxQD8u3XTjN5yd8Zs3BQY8r9YFUrYF8OQ3GxdYihsAjynOeImpdgG7UCAXF4zf-ZgNNmOBhG9gMckFDPclGVtcDO_xNdl9TMBLiqPzLkzYhAG7eV5D9HFytrrs8J0rG4Y741ezK5-jJ6PxGV48nOfo26ePXy8uydX15y8XH66IFaopxMiGSjYMXWepbUZotGK0bRVANyrDpFBKAJfHeqsG2qlBNwY4aD20XB-FFefozcm3vu_7Wr_Vzy5b8N4EiGvuedNqoSTltKKv_0Fv45rqFnZK6U61jdCV4ifKpphzgrFfkptN2npG-z26_hRdX6Prf0XXb1X06sF6Pc4w_JH8zqoC4gTk2goTpL-z_2P7E6MsqEg</recordid><startdate>20211001</startdate><enddate>20211001</enddate><creator>Zhang, Xin</creator><creator>Wang, Chen</creator><creator>Han, Qing</creator><creator>Chen, Xuan</creator><creator>Li, Guoyun</creator><creator>Yu, Guangli</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20211001</creationdate><title>Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation</title><author>Zhang, Xin ; Wang, Chen ; Han, Qing ; Chen, Xuan ; Li, Guoyun ; Yu, Guangli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-a45041dd88c0c5fe59710667ee8f7a143773e24b7107d087d95ae2e99d629b3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Antibiotics</topic><topic>Anticoagulants</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Chromatography</topic><topic>Cytotoxicity</topic><topic>Fourier transforms</topic><topic>Gastrointestinal tract</topic><topic>Glycopeptides</topic><topic>Heparin</topic><topic>Heparin - chemistry</topic><topic>Heparin - metabolism</topic><topic>High-performance liquid chromatography</topic><topic>Immunology</topic><topic>Immunomodulation</topic><topic>Intestinal Mucosa - chemistry</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestine</topic><topic>Life Sciences</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - immunology</topic><topic>Mass spectroscopy</topic><topic>Mice</topic><topic>Mucin</topic><topic>Mucins - chemistry</topic><topic>Mucins - pharmacology</topic><topic>Mucosa</topic><topic>Original Article</topic><topic>Pathology</topic><topic>Phagocytosis</topic><topic>Polysaccharides</topic><topic>RAW 264.7 Cells</topic><topic>Swine</topic><topic>Trypsin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Xin</creatorcontrib><creatorcontrib>Wang, Chen</creatorcontrib><creatorcontrib>Han, Qing</creatorcontrib><creatorcontrib>Chen, Xuan</creatorcontrib><creatorcontrib>Li, Guoyun</creatorcontrib><creatorcontrib>Yu, Guangli</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Glycoconjugate journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Xin</au><au>Wang, Chen</au><au>Han, Qing</au><au>Chen, Xuan</au><au>Li, Guoyun</au><au>Yu, Guangli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation</atitle><jtitle>Glycoconjugate journal</jtitle><stitle>Glycoconj J</stitle><addtitle>Glycoconj J</addtitle><date>2021-10-01</date><risdate>2021</risdate><volume>38</volume><issue>5</issue><spage>527</spage><epage>537</epage><pages>527-537</pages><issn>0282-0080</issn><eissn>1573-4986</eissn><abstract>Mucins are the major proteins that distributed on the intestinal mucosa layer and protect the intestine from pathogens infection. The composition of intestinal mucin
O
-glycans can affect the health of the gastrointestinal tract in pigs. Porcine intestinal mucosa is widely used as the main raw material of heparin extraction. The heparin extraction residues rich in mucins were usually wasted. The structure of mucin derived
O
-glycans in porcine intestinal mucosa are currently unknown. In this study, we isolated the mucins from the heparin extraction residues and profiled the
O
-glycans. After heparin extraction, mucin was digested with trypsin, and separated by strong anion exchange chromatography. The mucin derived
O
-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS). Thirty five kinds of
O
-glycans were identified, most of which were Core 3-derived glycans. In particular, the
O
-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total
O
-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon. The high content sialylated mucin may explain its effect in biological processes. Furthermore, the immunological activity results indicated that the porcine intestinal mucin could promote phagocytosis and proliferation without any cytotoxic effects, which may aid in the development of immunomodulators.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>34480673</pmid><doi>10.1007/s10719-021-10014-y</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Antibiotics Anticoagulants Biochemistry Biomedical and Life Sciences Chromatography Cytotoxicity Fourier transforms Gastrointestinal tract Glycopeptides Heparin Heparin - chemistry Heparin - metabolism High-performance liquid chromatography Immunology Immunomodulation Intestinal Mucosa - chemistry Intestinal Mucosa - metabolism Intestine Life Sciences Macrophages - drug effects Macrophages - immunology Mass spectroscopy Mice Mucin Mucins - chemistry Mucins - pharmacology Mucosa Original Article Pathology Phagocytosis Polysaccharides RAW 264.7 Cells Swine Trypsin |
| title | Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation |
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