Detection, Isolation, and Molecular Characterization of Escherichia albertii from Wild Birds in West Japan
Escherichia albertii is an emerging zoonotic foodborne pathogen. Several outbreaks of E. albertii have occurred, particularly in Japan. Although birds have been considered as one of the most important reservoirs of this bacterium, information regarding its prevalence in birds is still scarce. We per...
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| Veröffentlicht in: | Japanese Journal of Infectious Diseases 2022/03/31, Vol.75(2), pp.156-163 |
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| creator | Hinenoya, Atsushi Awasthi, Sharda Prasad Yasuda, Noritomo Nagano, Keigo Hassan, Jayedul Takehira, Keiji Hatanaka, Noritoshi Saito, Shun Watabe, Takashi Yoshizawa, Miki Inoue, Haruna Yamasaki, Shinji |
| description | Escherichia albertii is an emerging zoonotic foodborne pathogen. Several outbreaks of E. albertii have occurred, particularly in Japan. Although birds have been considered as one of the most important reservoirs of this bacterium, information regarding its prevalence in birds is still scarce. We performed a survey of E. albertii in wild birds in Japan and examined the characteristics of these isolates. E. albertii-specific genes were detected in five cloacal swabs from 156 birds by PCR. Four E. albertii strains were isolated from a swallow with two different E. albertii strains and two pigeons in a flock using XRM-MacConkey agar. These isolates were assigned to biogroup 3, showed no resistance to any tested antimicrobials, and were classified into two EAO-genotypes (EAOg2 and EAOg33) and were untypable. Similar to clinical E. albertii strains, these isolates carried virulence genes, including eae (n = 4), paa (n = 4), Eccdt-I (n = 2), and stx2f (n = 1), as well as Eacdt. Furthermore, stx2f genes in a strain were located on an inducible bacteriophage, which can confer the ability to produce Stx2f in E. coli. In conclusion, Japanese wild birds carried E. albertii at levels similar to the reported prevalence in birds. These isolates may have the potential to cause gastroenteritis in humans. |
| doi_str_mv | 10.7883/yoken.JJID.2021.355 |
| format | Article |
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Several outbreaks of E. albertii have occurred, particularly in Japan. Although birds have been considered as one of the most important reservoirs of this bacterium, information regarding its prevalence in birds is still scarce. We performed a survey of E. albertii in wild birds in Japan and examined the characteristics of these isolates. E. albertii-specific genes were detected in five cloacal swabs from 156 birds by PCR. Four E. albertii strains were isolated from a swallow with two different E. albertii strains and two pigeons in a flock using XRM-MacConkey agar. These isolates were assigned to biogroup 3, showed no resistance to any tested antimicrobials, and were classified into two EAO-genotypes (EAOg2 and EAOg33) and were untypable. Similar to clinical E. albertii strains, these isolates carried virulence genes, including eae (n = 4), paa (n = 4), Eccdt-I (n = 2), and stx2f (n = 1), as well as Eacdt. Furthermore, stx2f genes in a strain were located on an inducible bacteriophage, which can confer the ability to produce Stx2f in E. coli. In conclusion, Japanese wild birds carried E. albertii at levels similar to the reported prevalence in birds. These isolates may have the potential to cause gastroenteritis in humans.</description><identifier>ISSN: 1344-6304</identifier><identifier>EISSN: 1884-2836</identifier><identifier>DOI: 10.7883/yoken.JJID.2021.355</identifier><identifier>PMID: 34470969</identifier><language>eng</language><publisher>Japan: National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee</publisher><subject>Animals ; Antimicrobial agents ; Birds ; Culture Media ; E coli ; Escherichia - genetics ; Escherichia albertii ; Escherichia coli ; Foodborne pathogens ; Gastroenteritis ; Genes ; Genotypes ; Japan - epidemiology ; Phages ; pigeon ; Strains (organisms) ; Stx2f ; swallow ; Virulence ; wild bird</subject><ispartof>Japanese Journal of Infectious Diseases, 2022/03/31, Vol.75(2), pp.156-163</ispartof><rights>2022 Authors</rights><rights>Copyright Japan Science and Technology Agency 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c618t-b026851f4f246940ef6da38ad5045e865aa05cf7326e41fb22e2068e8acf8d5d3</citedby><cites>FETCH-LOGICAL-c618t-b026851f4f246940ef6da38ad5045e865aa05cf7326e41fb22e2068e8acf8d5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34470969$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hinenoya, Atsushi</creatorcontrib><creatorcontrib>Awasthi, Sharda Prasad</creatorcontrib><creatorcontrib>Yasuda, Noritomo</creatorcontrib><creatorcontrib>Nagano, Keigo</creatorcontrib><creatorcontrib>Hassan, Jayedul</creatorcontrib><creatorcontrib>Takehira, Keiji</creatorcontrib><creatorcontrib>Hatanaka, Noritoshi</creatorcontrib><creatorcontrib>Saito, Shun</creatorcontrib><creatorcontrib>Watabe, Takashi</creatorcontrib><creatorcontrib>Yoshizawa, Miki</creatorcontrib><creatorcontrib>Inoue, Haruna</creatorcontrib><creatorcontrib>Yamasaki, Shinji</creatorcontrib><title>Detection, Isolation, and Molecular Characterization of Escherichia albertii from Wild Birds in West Japan</title><title>Japanese Journal of Infectious Diseases</title><addtitle>Jpn J Infect Dis</addtitle><description>Escherichia albertii is an emerging zoonotic foodborne pathogen. Several outbreaks of E. albertii have occurred, particularly in Japan. Although birds have been considered as one of the most important reservoirs of this bacterium, information regarding its prevalence in birds is still scarce. We performed a survey of E. albertii in wild birds in Japan and examined the characteristics of these isolates. E. albertii-specific genes were detected in five cloacal swabs from 156 birds by PCR. Four E. albertii strains were isolated from a swallow with two different E. albertii strains and two pigeons in a flock using XRM-MacConkey agar. These isolates were assigned to biogroup 3, showed no resistance to any tested antimicrobials, and were classified into two EAO-genotypes (EAOg2 and EAOg33) and were untypable. Similar to clinical E. albertii strains, these isolates carried virulence genes, including eae (n = 4), paa (n = 4), Eccdt-I (n = 2), and stx2f (n = 1), as well as Eacdt. Furthermore, stx2f genes in a strain were located on an inducible bacteriophage, which can confer the ability to produce Stx2f in E. coli. In conclusion, Japanese wild birds carried E. albertii at levels similar to the reported prevalence in birds. These isolates may have the potential to cause gastroenteritis in humans.</description><subject>Animals</subject><subject>Antimicrobial agents</subject><subject>Birds</subject><subject>Culture Media</subject><subject>E coli</subject><subject>Escherichia - genetics</subject><subject>Escherichia albertii</subject><subject>Escherichia coli</subject><subject>Foodborne pathogens</subject><subject>Gastroenteritis</subject><subject>Genes</subject><subject>Genotypes</subject><subject>Japan - epidemiology</subject><subject>Phages</subject><subject>pigeon</subject><subject>Strains (organisms)</subject><subject>Stx2f</subject><subject>swallow</subject><subject>Virulence</subject><subject>wild bird</subject><issn>1344-6304</issn><issn>1884-2836</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1vEzEQhlcIREvhFyAhS1w4sMHf8R4hLZCoiAuoR2viHRMHZx3s3UP59ThNiAQX2xo_89qap2leMjqbGyPe3aefOMxWq-X1jFPOZkKpR80lM0a23Aj9uJ6FlK0WVF40z0rZUsqVYvRpc1Hrc9rp7rLZXuOIbgxpeEuWJUU4HmHoyZcU0U0RMllsIIMbMYffD_ckeXJT3KYW3CYAgbjGPIZAfE47chdiTz6E3BcSBnKHZSQr2MPwvHniIRZ8cdqvmu8fb74tPre3Xz8tF-9vW6eZGds15doo5qXnUneSotc9CAO9olKh0QqAKufngmuUzK85R061QQPOm1714qp5c8zd5_Rrqs_bXSgOY4QB01QsVzXfdJqair7-D92mKQ_1d5ZrqZnSVHaVEkfK5VRKRm_3Oewg31tG7UGFfVBhDyrsQYWtKmrXq1P2tN5hf-75O_sKLI_AtozwA88A1FG6iKfQubL8sPwTfmZcNWNxEH8Ax8igQg</recordid><startdate>20220331</startdate><enddate>20220331</enddate><creator>Hinenoya, Atsushi</creator><creator>Awasthi, Sharda Prasad</creator><creator>Yasuda, Noritomo</creator><creator>Nagano, Keigo</creator><creator>Hassan, Jayedul</creator><creator>Takehira, Keiji</creator><creator>Hatanaka, Noritoshi</creator><creator>Saito, Shun</creator><creator>Watabe, Takashi</creator><creator>Yoshizawa, Miki</creator><creator>Inoue, Haruna</creator><creator>Yamasaki, Shinji</creator><general>National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20220331</creationdate><title>Detection, Isolation, and Molecular Characterization of Escherichia albertii from Wild Birds in West Japan</title><author>Hinenoya, Atsushi ; 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Several outbreaks of E. albertii have occurred, particularly in Japan. Although birds have been considered as one of the most important reservoirs of this bacterium, information regarding its prevalence in birds is still scarce. We performed a survey of E. albertii in wild birds in Japan and examined the characteristics of these isolates. E. albertii-specific genes were detected in five cloacal swabs from 156 birds by PCR. Four E. albertii strains were isolated from a swallow with two different E. albertii strains and two pigeons in a flock using XRM-MacConkey agar. These isolates were assigned to biogroup 3, showed no resistance to any tested antimicrobials, and were classified into two EAO-genotypes (EAOg2 and EAOg33) and were untypable. Similar to clinical E. albertii strains, these isolates carried virulence genes, including eae (n = 4), paa (n = 4), Eccdt-I (n = 2), and stx2f (n = 1), as well as Eacdt. Furthermore, stx2f genes in a strain were located on an inducible bacteriophage, which can confer the ability to produce Stx2f in E. coli. In conclusion, Japanese wild birds carried E. albertii at levels similar to the reported prevalence in birds. These isolates may have the potential to cause gastroenteritis in humans.</abstract><cop>Japan</cop><pub>National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee</pub><pmid>34470969</pmid><doi>10.7883/yoken.JJID.2021.355</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Antimicrobial agents Birds Culture Media E coli Escherichia - genetics Escherichia albertii Escherichia coli Foodborne pathogens Gastroenteritis Genes Genotypes Japan - epidemiology Phages pigeon Strains (organisms) Stx2f swallow Virulence wild bird |
| title | Detection, Isolation, and Molecular Characterization of Escherichia albertii from Wild Birds in West Japan |
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