Clinically relevant concentration of propofol and benzodiazepines did not affect in vitro angiogenesis
Purpose Angiogenesis, one of regenerative medicine, is essential in the process of wound healing. The detailed effects of intravenous anesthetics and sedatives used during perioperative period have not yet been clarified. We investigated the effects of benzodiazepines and propofol on in vitro capill...
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| Veröffentlicht in: | Journal of anesthesia 2021-12, Vol.35 (6), p.870-878 |
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| creator | Takaishi, Kazumi Kudo, Yasusei Kawahito, Shinji Kitahata, Hiroshi |
| description | Purpose
Angiogenesis, one of regenerative medicine, is essential in the process of wound healing. The detailed effects of intravenous anesthetics and sedatives used during perioperative period have not yet been clarified. We investigated the effects of benzodiazepines and propofol on in vitro capillary tube formation.
Methods
The effects of midazolam, diazepam and propofol (1, 10, 50 µM each) on proliferation of human umbilical vein endothelial cells (HUVEC) and normal human diploid fibroblasts (NHDF) were determined. Quantitation of migration was achieved by measuring the fluorescence of migrating HUVEC using angiogenesis system. The effects of midazolam, diazepam and propofol on in vitro angiogenesis were investigated in co-cultured HUVEC and NHDF incubated. The effects of midazolam on activation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinases were examined by Western blot analysis using phospho-specific antibodies. Parametric data were analyzed with one-way repeated measures analysis of variance followed by the Scheffé test. A value of
P
|
| doi_str_mv | 10.1007/s00540-021-02993-x |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2566261614</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A682856490</galeid><sourcerecordid>A682856490</sourcerecordid><originalsourceid>FETCH-LOGICAL-c427t-184ba63a826cc334792417d4817d913cb38666ad476a119e29a497566dd5f583</originalsourceid><addsrcrecordid>eNp9kV9rHCEUxaW0NNttv0AfitCXvkyqo-M4j2HpPwj0Je_i6nUxuLrV2ZDk0-duJy0UliIq6u9cPPcQ8p6zS87Y-LkxNkjWsZ7jnCbR3b8gKy6F7rQYppdkxSYuOq2UviBvWrtljCnOxWtyIaRUeNIrEjYp5uhsSg-0QoI7m2fqSnaQ52rnWDItgR5qOZRQErXZ0y3kx-KjfYRDzNCoj57mMlMbAriZxkzv4lwLsrtYdoBIbG_Jq2BTg3fP-5rcfP1ys_neXf_89mNzdd052Y9zx7XcWiWs7pVzQshx6iUfvdS4oBe3FehGWS9HZTmfoJ-snMZBKe-HMGixJp-WsvjhX0dos9nH5iAlm6Ecm-kR7RVX2KQ1-bigO5vAxBwK-nUn3Fwp3etByYkh1Z2hTqaqTSVDiHj9D395hsfhYR_dWUG_CFwtrVUI5lDj3tYHw5k5pWyWlA2mbH6nbO5R9OHZ5nG7B_9X8idWBMQCNHzKO6jmthxrxs7_r-wTnhSxxw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2566261614</pqid></control><display><type>article</type><title>Clinically relevant concentration of propofol and benzodiazepines did not affect in vitro angiogenesis</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Takaishi, Kazumi ; Kudo, Yasusei ; Kawahito, Shinji ; Kitahata, Hiroshi</creator><creatorcontrib>Takaishi, Kazumi ; Kudo, Yasusei ; Kawahito, Shinji ; Kitahata, Hiroshi</creatorcontrib><description>Purpose
Angiogenesis, one of regenerative medicine, is essential in the process of wound healing. The detailed effects of intravenous anesthetics and sedatives used during perioperative period have not yet been clarified. We investigated the effects of benzodiazepines and propofol on in vitro capillary tube formation.
Methods
The effects of midazolam, diazepam and propofol (1, 10, 50 µM each) on proliferation of human umbilical vein endothelial cells (HUVEC) and normal human diploid fibroblasts (NHDF) were determined. Quantitation of migration was achieved by measuring the fluorescence of migrating HUVEC using angiogenesis system. The effects of midazolam, diazepam and propofol on in vitro angiogenesis were investigated in co-cultured HUVEC and NHDF incubated. The effects of midazolam on activation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinases were examined by Western blot analysis using phospho-specific antibodies. Parametric data were analyzed with one-way repeated measures analysis of variance followed by the Scheffé test. A value of
P
< 0.05 was considered statistically significant.
Results
Fifty µM of midazolam significantly impaired endothelial cell proliferation, migration, and in vitro capillary tube formation. Propofol, diazepam or lower dose midazolam did not show any enhancing or suppressive effects on in vitro angiogenesis. Fifty µM of midazolam remarkably activated ERK, but not p38 MAPK in HUVEC.
Conclusion
Propofol and benzodiazepines except high-dose midazolam did not affect in vitro angiogenesis. High-dose midazolam may impair in vitro capillary tube formation due to by suppressing proliferation and migration of endothelial cells via activation of ERK.</description><identifier>ISSN: 0913-8668</identifier><identifier>EISSN: 1438-8359</identifier><identifier>DOI: 10.1007/s00540-021-02993-x</identifier><identifier>PMID: 34460008</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>Analysis ; Anesthesiology ; Anesthetics ; Anesthetics, Intravenous ; Antibodies ; Aprotinin ; Benzodiazepines ; Critical Care Medicine ; Diazepam ; Emergency Medicine ; Endothelium ; Ethylenediaminetetraacetic acid ; Fluorescence ; Human Umbilical Vein Endothelial Cells ; Humans ; Intensive ; Medicine ; Medicine & Public Health ; Midazolam ; Mitogens ; Original Article ; Pain Medicine ; Phenols ; Propofol - pharmacology ; Protein kinases ; Suramin sodium ; Textile industry ; Viral antibodies</subject><ispartof>Journal of anesthesia, 2021-12, Vol.35 (6), p.870-878</ispartof><rights>Japanese Society of Anesthesiologists 2021</rights><rights>2021. Japanese Society of Anesthesiologists.</rights><rights>COPYRIGHT 2021 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c427t-184ba63a826cc334792417d4817d913cb38666ad476a119e29a497566dd5f583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00540-021-02993-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00540-021-02993-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34460008$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takaishi, Kazumi</creatorcontrib><creatorcontrib>Kudo, Yasusei</creatorcontrib><creatorcontrib>Kawahito, Shinji</creatorcontrib><creatorcontrib>Kitahata, Hiroshi</creatorcontrib><title>Clinically relevant concentration of propofol and benzodiazepines did not affect in vitro angiogenesis</title><title>Journal of anesthesia</title><addtitle>J Anesth</addtitle><addtitle>J Anesth</addtitle><description>Purpose
Angiogenesis, one of regenerative medicine, is essential in the process of wound healing. The detailed effects of intravenous anesthetics and sedatives used during perioperative period have not yet been clarified. We investigated the effects of benzodiazepines and propofol on in vitro capillary tube formation.
Methods
The effects of midazolam, diazepam and propofol (1, 10, 50 µM each) on proliferation of human umbilical vein endothelial cells (HUVEC) and normal human diploid fibroblasts (NHDF) were determined. Quantitation of migration was achieved by measuring the fluorescence of migrating HUVEC using angiogenesis system. The effects of midazolam, diazepam and propofol on in vitro angiogenesis were investigated in co-cultured HUVEC and NHDF incubated. The effects of midazolam on activation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinases were examined by Western blot analysis using phospho-specific antibodies. Parametric data were analyzed with one-way repeated measures analysis of variance followed by the Scheffé test. A value of
P
< 0.05 was considered statistically significant.
Results
Fifty µM of midazolam significantly impaired endothelial cell proliferation, migration, and in vitro capillary tube formation. Propofol, diazepam or lower dose midazolam did not show any enhancing or suppressive effects on in vitro angiogenesis. Fifty µM of midazolam remarkably activated ERK, but not p38 MAPK in HUVEC.
Conclusion
Propofol and benzodiazepines except high-dose midazolam did not affect in vitro angiogenesis. High-dose midazolam may impair in vitro capillary tube formation due to by suppressing proliferation and migration of endothelial cells via activation of ERK.</description><subject>Analysis</subject><subject>Anesthesiology</subject><subject>Anesthetics</subject><subject>Anesthetics, Intravenous</subject><subject>Antibodies</subject><subject>Aprotinin</subject><subject>Benzodiazepines</subject><subject>Critical Care Medicine</subject><subject>Diazepam</subject><subject>Emergency Medicine</subject><subject>Endothelium</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Fluorescence</subject><subject>Human Umbilical Vein Endothelial Cells</subject><subject>Humans</subject><subject>Intensive</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Midazolam</subject><subject>Mitogens</subject><subject>Original Article</subject><subject>Pain Medicine</subject><subject>Phenols</subject><subject>Propofol - pharmacology</subject><subject>Protein kinases</subject><subject>Suramin sodium</subject><subject>Textile industry</subject><subject>Viral antibodies</subject><issn>0913-8668</issn><issn>1438-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV9rHCEUxaW0NNttv0AfitCXvkyqo-M4j2HpPwj0Je_i6nUxuLrV2ZDk0-duJy0UliIq6u9cPPcQ8p6zS87Y-LkxNkjWsZ7jnCbR3b8gKy6F7rQYppdkxSYuOq2UviBvWrtljCnOxWtyIaRUeNIrEjYp5uhsSg-0QoI7m2fqSnaQ52rnWDItgR5qOZRQErXZ0y3kx-KjfYRDzNCoj57mMlMbAriZxkzv4lwLsrtYdoBIbG_Jq2BTg3fP-5rcfP1ys_neXf_89mNzdd052Y9zx7XcWiWs7pVzQshx6iUfvdS4oBe3FehGWS9HZTmfoJ-snMZBKe-HMGixJp-WsvjhX0dos9nH5iAlm6Ecm-kR7RVX2KQ1-bigO5vAxBwK-nUn3Fwp3etByYkh1Z2hTqaqTSVDiHj9D395hsfhYR_dWUG_CFwtrVUI5lDj3tYHw5k5pWyWlA2mbH6nbO5R9OHZ5nG7B_9X8idWBMQCNHzKO6jmthxrxs7_r-wTnhSxxw</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Takaishi, Kazumi</creator><creator>Kudo, Yasusei</creator><creator>Kawahito, Shinji</creator><creator>Kitahata, Hiroshi</creator><general>Springer Singapore</general><general>Springer</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20211201</creationdate><title>Clinically relevant concentration of propofol and benzodiazepines did not affect in vitro angiogenesis</title><author>Takaishi, Kazumi ; Kudo, Yasusei ; Kawahito, Shinji ; Kitahata, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-184ba63a826cc334792417d4817d913cb38666ad476a119e29a497566dd5f583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Analysis</topic><topic>Anesthesiology</topic><topic>Anesthetics</topic><topic>Anesthetics, Intravenous</topic><topic>Antibodies</topic><topic>Aprotinin</topic><topic>Benzodiazepines</topic><topic>Critical Care Medicine</topic><topic>Diazepam</topic><topic>Emergency Medicine</topic><topic>Endothelium</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Fluorescence</topic><topic>Human Umbilical Vein Endothelial Cells</topic><topic>Humans</topic><topic>Intensive</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Midazolam</topic><topic>Mitogens</topic><topic>Original Article</topic><topic>Pain Medicine</topic><topic>Phenols</topic><topic>Propofol - pharmacology</topic><topic>Protein kinases</topic><topic>Suramin sodium</topic><topic>Textile industry</topic><topic>Viral antibodies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takaishi, Kazumi</creatorcontrib><creatorcontrib>Kudo, Yasusei</creatorcontrib><creatorcontrib>Kawahito, Shinji</creatorcontrib><creatorcontrib>Kitahata, Hiroshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of anesthesia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takaishi, Kazumi</au><au>Kudo, Yasusei</au><au>Kawahito, Shinji</au><au>Kitahata, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinically relevant concentration of propofol and benzodiazepines did not affect in vitro angiogenesis</atitle><jtitle>Journal of anesthesia</jtitle><stitle>J Anesth</stitle><addtitle>J Anesth</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>35</volume><issue>6</issue><spage>870</spage><epage>878</epage><pages>870-878</pages><issn>0913-8668</issn><eissn>1438-8359</eissn><abstract>Purpose
Angiogenesis, one of regenerative medicine, is essential in the process of wound healing. The detailed effects of intravenous anesthetics and sedatives used during perioperative period have not yet been clarified. We investigated the effects of benzodiazepines and propofol on in vitro capillary tube formation.
Methods
The effects of midazolam, diazepam and propofol (1, 10, 50 µM each) on proliferation of human umbilical vein endothelial cells (HUVEC) and normal human diploid fibroblasts (NHDF) were determined. Quantitation of migration was achieved by measuring the fluorescence of migrating HUVEC using angiogenesis system. The effects of midazolam, diazepam and propofol on in vitro angiogenesis were investigated in co-cultured HUVEC and NHDF incubated. The effects of midazolam on activation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinases were examined by Western blot analysis using phospho-specific antibodies. Parametric data were analyzed with one-way repeated measures analysis of variance followed by the Scheffé test. A value of
P
< 0.05 was considered statistically significant.
Results
Fifty µM of midazolam significantly impaired endothelial cell proliferation, migration, and in vitro capillary tube formation. Propofol, diazepam or lower dose midazolam did not show any enhancing or suppressive effects on in vitro angiogenesis. Fifty µM of midazolam remarkably activated ERK, but not p38 MAPK in HUVEC.
Conclusion
Propofol and benzodiazepines except high-dose midazolam did not affect in vitro angiogenesis. High-dose midazolam may impair in vitro capillary tube formation due to by suppressing proliferation and migration of endothelial cells via activation of ERK.</abstract><cop>Singapore</cop><pub>Springer Singapore</pub><pmid>34460008</pmid><doi>10.1007/s00540-021-02993-x</doi><tpages>9</tpages></addata></record> |
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| subjects | Analysis Anesthesiology Anesthetics Anesthetics, Intravenous Antibodies Aprotinin Benzodiazepines Critical Care Medicine Diazepam Emergency Medicine Endothelium Ethylenediaminetetraacetic acid Fluorescence Human Umbilical Vein Endothelial Cells Humans Intensive Medicine Medicine & Public Health Midazolam Mitogens Original Article Pain Medicine Phenols Propofol - pharmacology Protein kinases Suramin sodium Textile industry Viral antibodies |
| title | Clinically relevant concentration of propofol and benzodiazepines did not affect in vitro angiogenesis |
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