Crystal Structure of a Sucrose-6-phosphate Hydrolase from Lactobacillus gasseri with Potential Applications in Fructan Production and the Food Industry

Crystal Structure of a Sucrose-6-phosphate Hydrolase from Lactobacillus gasseri with Potential Applications in Fructan Production and the Food Industry

Fructooligosaccharides (FOSs) are polymers of fructose with a prebiotic activity because of their production and fermentation by bacteria that inhabit the gastrointestinal tract and are widely used in the industry and new functional foods. Lactobacillus gasseri stands out as an important homoferment...

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Veröffentlicht in:Journal of agricultural and food chemistry 2021-09, Vol.69 (35), p.10223-10234
Hauptverfasser: de Lima, Mariana Z. T, de Almeida, Leonardo R, Mera, Alain M, Bernardes, Amanda, Garcia, Wanius, Muniz, João R. C
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Biotechnology and Biological Transformations
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container_end_page 10234
container_issue 35
container_start_page 10223
container_title Journal of agricultural and food chemistry
container_volume 69
creator de Lima, Mariana Z. T
de Almeida, Leonardo R
Mera, Alain M
Bernardes, Amanda
Garcia, Wanius
Muniz, João R. C
description Fructooligosaccharides (FOSs) are polymers of fructose with a prebiotic activity because of their production and fermentation by bacteria that inhabit the gastrointestinal tract and are widely used in the industry and new functional foods. Lactobacillus gasseri stands out as an important homofermentative microorganism related to FOS production, and its potential applications in the industry are undeniable. In this study, we report the production and characterization of a sucrose-6-phosphate hydrolase from L. gasseri belonging to the GH32 family. Apo-LgAs32 and LgAs32 complexed with β-d-fructose structures were determined at a resolution of 1.94 and 1.84 Å, respectively. The production of FOS, fructans, 1-kestose, and nystose by the recombinant LgAs32, using sucrose as a substrate, shown in this study is very promising. When compared to its homologous enzyme from Lactobacillus reuteri, the production of 1-kestose by LgAs32 is increased; thus, LgAs32 can be considered as an alternative in fructan production and other industrial applications.
doi_str_mv 10.1021/acs.jafc.1c03901
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title Crystal Structure of a Sucrose-6-phosphate Hydrolase from Lactobacillus gasseri with Potential Applications in Fructan Production and the Food Industry
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