The Clinical Significance and Functional Role of miR-466 in Gastric Cancer Peritoneal Metastasis
The prognosis of metastasis gastric cancer patients remains poor and the identification of novel molecular markers will improve the management of gastric cancer patients. The present study aimed to investigate the clinical significance and functional role of miR-466 in gastric cancer peritoneal meta...
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| Veröffentlicht in: | Molecular biotechnology 2022, Vol.64 (1), p.25-32 |
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| creator | Wang, Yong Yan, Bo Ni, Lei Si, Yunfei Cao, Pingli |
| description | The prognosis of metastasis gastric cancer patients remains poor and the identification of novel molecular markers will improve the management of gastric cancer patients. The present study aimed to investigate the clinical significance and functional role of miR-466 in gastric cancer peritoneal metastasis. miR-466 expression was confirmed by RT-qPCR. The biological functions were examined by MTT assay, Transwell migration, and invasion assays. The Kaplan–Meier survival curve and multivariate Cox regression analysis were used to investigate the clinical role of miR-466. The logistic regression analysis was performed to reveal the risk factors associated with peritoneal metastasis. miR-466 expression was downregulated in gastric cancer cell lines, tumor tissues, and peritoneal metastasis tissues compared with respective controls. Increased miR-466 expression inhibited proliferation, migration, and invasion of gastric cancer cells. Besides, the lower expression of miR-466 in gastric cancer patients was associated with peritoneal dissemination. Furthermore, multivariate Cox proportional hazard regression analyses demonstrated miR-466 expression level as an independent predictor of prognosis of gastric cancer. The present study provides novel evidence for the clinical and biological significance of miR-466 expression as a possible biomarker for the prognosis and identifying patients with peritoneal metastasis, as well as a potential therapeutic target in patients with gastric cancer. |
| doi_str_mv | 10.1007/s12033-021-00382-z |
| format | Article |
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The present study aimed to investigate the clinical significance and functional role of miR-466 in gastric cancer peritoneal metastasis. miR-466 expression was confirmed by RT-qPCR. The biological functions were examined by MTT assay, Transwell migration, and invasion assays. The Kaplan–Meier survival curve and multivariate Cox regression analysis were used to investigate the clinical role of miR-466. The logistic regression analysis was performed to reveal the risk factors associated with peritoneal metastasis. miR-466 expression was downregulated in gastric cancer cell lines, tumor tissues, and peritoneal metastasis tissues compared with respective controls. Increased miR-466 expression inhibited proliferation, migration, and invasion of gastric cancer cells. Besides, the lower expression of miR-466 in gastric cancer patients was associated with peritoneal dissemination. Furthermore, multivariate Cox proportional hazard regression analyses demonstrated miR-466 expression level as an independent predictor of prognosis of gastric cancer. The present study provides novel evidence for the clinical and biological significance of miR-466 expression as a possible biomarker for the prognosis and identifying patients with peritoneal metastasis, as well as a potential therapeutic target in patients with gastric cancer.</description><identifier>ISSN: 1073-6085</identifier><identifier>EISSN: 1559-0305</identifier><identifier>DOI: 10.1007/s12033-021-00382-z</identifier><identifier>PMID: 34435325</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biochemistry ; Biological Techniques ; Biomarkers ; Biomarkers, Tumor - genetics ; Biotechnology ; Cancer ; Cell Biology ; Cell Line, Tumor ; Cell Movement - genetics ; Cell proliferation ; Cell Proliferation - genetics ; Chemistry ; Chemistry and Materials Science ; Clinical significance ; Down-Regulation ; Female ; Gastric cancer ; Human Genetics ; Humans ; Male ; Medical prognosis ; Metastases ; Metastasis ; MicroRNAs - genetics ; Middle Aged ; Multivariate analysis ; Original Paper ; Patients ; Peritoneal Neoplasms - genetics ; Peritoneal Neoplasms - secondary ; Peritoneum ; Prognosis ; Protein Science ; Regression analysis ; Risk analysis ; Risk Factors ; Stomach Neoplasms - genetics ; Stomach Neoplasms - pathology ; Therapeutic targets ; Tumor cell lines ; Tumors</subject><ispartof>Molecular biotechnology, 2022, Vol.64 (1), p.25-32</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-8b965c33e1c0c444230222596f5bf8516971160c8d2362083b5ccd860be8c1a73</cites><orcidid>0000-0002-3984-963X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12033-021-00382-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12033-021-00382-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34435325$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yong</creatorcontrib><creatorcontrib>Yan, Bo</creatorcontrib><creatorcontrib>Ni, Lei</creatorcontrib><creatorcontrib>Si, Yunfei</creatorcontrib><creatorcontrib>Cao, Pingli</creatorcontrib><title>The Clinical Significance and Functional Role of miR-466 in Gastric Cancer Peritoneal Metastasis</title><title>Molecular biotechnology</title><addtitle>Mol Biotechnol</addtitle><addtitle>Mol Biotechnol</addtitle><description>The prognosis of metastasis gastric cancer patients remains poor and the identification of novel molecular markers will improve the management of gastric cancer patients. The present study aimed to investigate the clinical significance and functional role of miR-466 in gastric cancer peritoneal metastasis. miR-466 expression was confirmed by RT-qPCR. The biological functions were examined by MTT assay, Transwell migration, and invasion assays. The Kaplan–Meier survival curve and multivariate Cox regression analysis were used to investigate the clinical role of miR-466. The logistic regression analysis was performed to reveal the risk factors associated with peritoneal metastasis. miR-466 expression was downregulated in gastric cancer cell lines, tumor tissues, and peritoneal metastasis tissues compared with respective controls. Increased miR-466 expression inhibited proliferation, migration, and invasion of gastric cancer cells. Besides, the lower expression of miR-466 in gastric cancer patients was associated with peritoneal dissemination. Furthermore, multivariate Cox proportional hazard regression analyses demonstrated miR-466 expression level as an independent predictor of prognosis of gastric cancer. The present study provides novel evidence for the clinical and biological significance of miR-466 expression as a possible biomarker for the prognosis and identifying patients with peritoneal metastasis, as well as a potential therapeutic target in patients with gastric cancer.</description><subject>Biochemistry</subject><subject>Biological Techniques</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biotechnology</subject><subject>Cancer</subject><subject>Cell Biology</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - genetics</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - genetics</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Clinical significance</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Gastric cancer</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>MicroRNAs - 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Academic</collection><jtitle>Molecular biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yong</au><au>Yan, Bo</au><au>Ni, Lei</au><au>Si, Yunfei</au><au>Cao, Pingli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Clinical Significance and Functional Role of miR-466 in Gastric Cancer Peritoneal Metastasis</atitle><jtitle>Molecular biotechnology</jtitle><stitle>Mol Biotechnol</stitle><addtitle>Mol Biotechnol</addtitle><date>2022</date><risdate>2022</risdate><volume>64</volume><issue>1</issue><spage>25</spage><epage>32</epage><pages>25-32</pages><issn>1073-6085</issn><eissn>1559-0305</eissn><abstract>The prognosis of metastasis gastric cancer patients remains poor and the identification of novel molecular markers will improve the management of gastric cancer patients. The present study aimed to investigate the clinical significance and functional role of miR-466 in gastric cancer peritoneal metastasis. miR-466 expression was confirmed by RT-qPCR. The biological functions were examined by MTT assay, Transwell migration, and invasion assays. The Kaplan–Meier survival curve and multivariate Cox regression analysis were used to investigate the clinical role of miR-466. The logistic regression analysis was performed to reveal the risk factors associated with peritoneal metastasis. miR-466 expression was downregulated in gastric cancer cell lines, tumor tissues, and peritoneal metastasis tissues compared with respective controls. Increased miR-466 expression inhibited proliferation, migration, and invasion of gastric cancer cells. Besides, the lower expression of miR-466 in gastric cancer patients was associated with peritoneal dissemination. Furthermore, multivariate Cox proportional hazard regression analyses demonstrated miR-466 expression level as an independent predictor of prognosis of gastric cancer. The present study provides novel evidence for the clinical and biological significance of miR-466 expression as a possible biomarker for the prognosis and identifying patients with peritoneal metastasis, as well as a potential therapeutic target in patients with gastric cancer.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>34435325</pmid><doi>10.1007/s12033-021-00382-z</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-3984-963X</orcidid></addata></record> |
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| subjects | Biochemistry Biological Techniques Biomarkers Biomarkers, Tumor - genetics Biotechnology Cancer Cell Biology Cell Line, Tumor Cell Movement - genetics Cell proliferation Cell Proliferation - genetics Chemistry Chemistry and Materials Science Clinical significance Down-Regulation Female Gastric cancer Human Genetics Humans Male Medical prognosis Metastases Metastasis MicroRNAs - genetics Middle Aged Multivariate analysis Original Paper Patients Peritoneal Neoplasms - genetics Peritoneal Neoplasms - secondary Peritoneum Prognosis Protein Science Regression analysis Risk analysis Risk Factors Stomach Neoplasms - genetics Stomach Neoplasms - pathology Therapeutic targets Tumor cell lines Tumors |
| title | The Clinical Significance and Functional Role of miR-466 in Gastric Cancer Peritoneal Metastasis |
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