Graphene nanofiber composites for enhanced neuronal differentiation of human mesenchymal stem cells

To differentiate mesenchymal stem cells into functional dopaminergic neurons using an electrospun polycaprolactone (PCL) and graphene (G) nanocomposite. A one-step approach was used to electrospin the PCL nanocomposite, with varying G concentrations, followed by evaluating their biocompatibility and...

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Veröffentlicht in:	Nanomedicine (London, England) England), 2021-09, Vol.16 (22), p.1963-1982
Hauptverfasser:	Rawat, Sonali, Jain, Krishan Gopal, Gupta, Deepika, Raghav, Pawan Kumar, Chaudhuri, Rituparna, Pinky, Shakeel, Adeeba, Arora, Varun, Sharma, Harshita, Debnath, Debika, Kalluri, Ankarao, Agrawal, Ashwini K., Jassal, Manjeet, Dinda, Amit K., Patra, Prabir, Mohanty, Sujata
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Sprache:	eng
Schlagworte:	dopaminergic neurons electrospinning graphene mesenchymal stem cells nanofibers neuronal differentiation
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container_title	Nanomedicine (London, England)
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creator	Rawat, Sonali Jain, Krishan Gopal Gupta, Deepika Raghav, Pawan Kumar Chaudhuri, Rituparna Pinky Shakeel, Adeeba Arora, Varun Sharma, Harshita Debnath, Debika Kalluri, Ankarao Agrawal, Ashwini K. Jassal, Manjeet Dinda, Amit K. Patra, Prabir Mohanty, Sujata
description	To differentiate mesenchymal stem cells into functional dopaminergic neurons using an electrospun polycaprolactone (PCL) and graphene (G) nanocomposite. A one-step approach was used to electrospin the PCL nanocomposite, with varying G concentrations, followed by evaluating their biocompatibility and neuronal differentiation. PCL with exiguous graphene demonstrated an ideal nanotopography with an unprecedented combination of guidance stimuli and substrate cues, aiding the enhanced differentiation of mesenchymal stem cells into dopaminergic neurons. These newly differentiated neurons were seen to exhibit unique neuronal arborization, enhanced intracellular Ca influx and dopamine secretion. Having cost-effective fabrication and room-temperature storage, the PCL-G nanocomposites could pave the way for enhanced neuronal differentiation, thereby opening a new horizon for an array of applications in neural regenerative medicine.
doi_str_mv	10.2217/nnm-2021-0121
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subjects	dopaminergic neurons electrospinning graphene mesenchymal stem cells nanofibers neuronal differentiation
title	Graphene nanofiber composites for enhanced neuronal differentiation of human mesenchymal stem cells
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