Enhanced SA and Ca2+ signaling results in PCD-mediated spontaneous leaf necrosis in wheat mutant wsl
Leaf is the major photosynthesis organ and the key source of wheat ( Triticum aestivum L.) grain. Spotted leaf ( spl ) mutant is a kind of leaf lesion mimic mutants (LMMs) in plants, which is an ideal material for studying the mechanisms of leaf development. In this study, we report the leaf abnorma...
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| creator | Li, Huijuan Jiao, Zhixin Zhang, Peipei Ni, Yongjing Wang, Ting Zhang, Jing Li, Junchang Jiang, Yumei Yang, Xiwen Li, Lei Yao, Ziping Niu, Jishan He, Dexian |
| description | Leaf is the major photosynthesis organ and the key source of wheat (
Triticum aestivum
L.) grain. Spotted leaf (
spl
) mutant is a kind of leaf lesion mimic mutants (LMMs) in plants, which is an ideal material for studying the mechanisms of leaf development. In this study, we report the leaf abnormal development molecular mechanism of a
spl
mutant named
white stripe leaf
(
wsl
) derived from wheat cultivar Guomai 301 (WT). Histochemical observation indicated that the leaf mesophyll cells of the
wsl
were destroyed in the necrosis regions. To explore the molecular regulatory network of the leaf development in mutant
wsl
, we employed transcriptome analysis, histochemistry, quantitative real-time PCR (qRT-PCR), and observations of the key metabolites and photosynthesis parameters. Compared to WT, the expressions of the chlorophyll synthesis and photosynthesis-related homeotic genes were repressed; many genes in the WRKY transcription factor (TF) families were highly expressed; the salicylic acid (SA) and Ca
2+
signal transductions were enhanced in
wsl
. Both the chlorophyll contents and the photosynthesis rate were lower in
wsl
. The contents of SA and reactive oxygen species (ROS) were significantly higher, and the leaf rust resistance was enhanced in
wsl
. Based on the experimental data, a primary molecular regulatory model for leaf development in
wsl
was established. The results indicated that the SA accumulation and enhanced Ca
2+
signaling led to programmed cell death (PCD), and ultimately resulted in spontaneous leaf necrosis of
wsl.
These results laid a solid foundation for further research on the molecular mechanism of leaf development in wheat. |
| doi_str_mv | 10.1007/s00438-021-01811-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2564136122</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2586187294</sourcerecordid><originalsourceid>FETCH-LOGICAL-c282t-3e3881e4024ce43f23d1068ca52ee2b955ac494170be350584b1aa8e2d3289c13</originalsourceid><addsrcrecordid>eNp90E1Lw0AQBuBFFKzVP-BpwYsg0Z3ZTbI5llo_QFBQz8s2mbQpyaZmE4r99aaNKHjwtHN43mHnZewcxDUIEd94IZTUgUAIBGiAYHvARhBBHKgI5eHPDOExO_F-JQTEEcYjls3c0rqUMv464dZlfGrxivti4WxZuAVvyHdl63nh-Mv0NqgoK2zba7-uXWsd1Z3nJdmcO0qb2hd7uVmSbXnV9aDlG1-esqPclp7Ovt8xe7-bvU0fgqfn-8fp5ClIUWMbSJJaAymBKiUlc5QZiEinNkQinCdhaFOVKIjFnGQoQq3mYK0mzCTqJAU5ZpfD3nVTf3TkW1MVPqWyHD5qMIwUyAgQe3rxh67qrumP3ikdgY4xUb3CQe1u8w3lZt0UlW0-DQizK94MxZu-eLMv3mz7kBxCvsduQc3v6n9SX-guhOw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2586187294</pqid></control><display><type>article</type><title>Enhanced SA and Ca2+ signaling results in PCD-mediated spontaneous leaf necrosis in wheat mutant wsl</title><source>SpringerLink Journals - AutoHoldings</source><creator>Li, Huijuan ; Jiao, Zhixin ; Zhang, Peipei ; Ni, Yongjing ; Wang, Ting ; Zhang, Jing ; Li, Junchang ; Jiang, Yumei ; Yang, Xiwen ; Li, Lei ; Yao, Ziping ; Niu, Jishan ; He, Dexian</creator><creatorcontrib>Li, Huijuan ; Jiao, Zhixin ; Zhang, Peipei ; Ni, Yongjing ; Wang, Ting ; Zhang, Jing ; Li, Junchang ; Jiang, Yumei ; Yang, Xiwen ; Li, Lei ; Yao, Ziping ; Niu, Jishan ; He, Dexian</creatorcontrib><description>Leaf is the major photosynthesis organ and the key source of wheat (
Triticum aestivum
L.) grain. Spotted leaf (
spl
) mutant is a kind of leaf lesion mimic mutants (LMMs) in plants, which is an ideal material for studying the mechanisms of leaf development. In this study, we report the leaf abnormal development molecular mechanism of a
spl
mutant named
white stripe leaf
(
wsl
) derived from wheat cultivar Guomai 301 (WT). Histochemical observation indicated that the leaf mesophyll cells of the
wsl
were destroyed in the necrosis regions. To explore the molecular regulatory network of the leaf development in mutant
wsl
, we employed transcriptome analysis, histochemistry, quantitative real-time PCR (qRT-PCR), and observations of the key metabolites and photosynthesis parameters. Compared to WT, the expressions of the chlorophyll synthesis and photosynthesis-related homeotic genes were repressed; many genes in the WRKY transcription factor (TF) families were highly expressed; the salicylic acid (SA) and Ca
2+
signal transductions were enhanced in
wsl
. Both the chlorophyll contents and the photosynthesis rate were lower in
wsl
. The contents of SA and reactive oxygen species (ROS) were significantly higher, and the leaf rust resistance was enhanced in
wsl
. Based on the experimental data, a primary molecular regulatory model for leaf development in
wsl
was established. The results indicated that the SA accumulation and enhanced Ca
2+
signaling led to programmed cell death (PCD), and ultimately resulted in spontaneous leaf necrosis of
wsl.
These results laid a solid foundation for further research on the molecular mechanism of leaf development in wheat.</description><identifier>ISSN: 1617-4615</identifier><identifier>EISSN: 1617-4623</identifier><identifier>DOI: 10.1007/s00438-021-01811-z</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animal Genetics and Genomics ; Apoptosis ; Biochemistry ; Biomedical and Life Sciences ; Calcium signalling ; Cell death ; Chlorophyll ; Cultivars ; Human Genetics ; Leaf rust ; Life Sciences ; Mesophyll ; Microbial Genetics and Genomics ; Mutants ; Necrosis ; Original Article ; Photosynthesis ; Plant Genetics and Genomics ; Reactive oxygen species ; Salicylic acid ; Transcriptomes ; Triticum aestivum</subject><ispartof>Molecular genetics and genomics : MGG, 2021-11, Vol.296 (6), p.1249-1262</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-3e3881e4024ce43f23d1068ca52ee2b955ac494170be350584b1aa8e2d3289c13</citedby><cites>FETCH-LOGICAL-c282t-3e3881e4024ce43f23d1068ca52ee2b955ac494170be350584b1aa8e2d3289c13</cites><orcidid>0000-0002-7233-0442</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00438-021-01811-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00438-021-01811-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Li, Huijuan</creatorcontrib><creatorcontrib>Jiao, Zhixin</creatorcontrib><creatorcontrib>Zhang, Peipei</creatorcontrib><creatorcontrib>Ni, Yongjing</creatorcontrib><creatorcontrib>Wang, Ting</creatorcontrib><creatorcontrib>Zhang, Jing</creatorcontrib><creatorcontrib>Li, Junchang</creatorcontrib><creatorcontrib>Jiang, Yumei</creatorcontrib><creatorcontrib>Yang, Xiwen</creatorcontrib><creatorcontrib>Li, Lei</creatorcontrib><creatorcontrib>Yao, Ziping</creatorcontrib><creatorcontrib>Niu, Jishan</creatorcontrib><creatorcontrib>He, Dexian</creatorcontrib><title>Enhanced SA and Ca2+ signaling results in PCD-mediated spontaneous leaf necrosis in wheat mutant wsl</title><title>Molecular genetics and genomics : MGG</title><addtitle>Mol Genet Genomics</addtitle><description>Leaf is the major photosynthesis organ and the key source of wheat (
Triticum aestivum
L.) grain. Spotted leaf (
spl
) mutant is a kind of leaf lesion mimic mutants (LMMs) in plants, which is an ideal material for studying the mechanisms of leaf development. In this study, we report the leaf abnormal development molecular mechanism of a
spl
mutant named
white stripe leaf
(
wsl
) derived from wheat cultivar Guomai 301 (WT). Histochemical observation indicated that the leaf mesophyll cells of the
wsl
were destroyed in the necrosis regions. To explore the molecular regulatory network of the leaf development in mutant
wsl
, we employed transcriptome analysis, histochemistry, quantitative real-time PCR (qRT-PCR), and observations of the key metabolites and photosynthesis parameters. Compared to WT, the expressions of the chlorophyll synthesis and photosynthesis-related homeotic genes were repressed; many genes in the WRKY transcription factor (TF) families were highly expressed; the salicylic acid (SA) and Ca
2+
signal transductions were enhanced in
wsl
. Both the chlorophyll contents and the photosynthesis rate were lower in
wsl
. The contents of SA and reactive oxygen species (ROS) were significantly higher, and the leaf rust resistance was enhanced in
wsl
. Based on the experimental data, a primary molecular regulatory model for leaf development in
wsl
was established. The results indicated that the SA accumulation and enhanced Ca
2+
signaling led to programmed cell death (PCD), and ultimately resulted in spontaneous leaf necrosis of
wsl.
These results laid a solid foundation for further research on the molecular mechanism of leaf development in wheat.</description><subject>Animal Genetics and Genomics</subject><subject>Apoptosis</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Calcium signalling</subject><subject>Cell death</subject><subject>Chlorophyll</subject><subject>Cultivars</subject><subject>Human Genetics</subject><subject>Leaf rust</subject><subject>Life Sciences</subject><subject>Mesophyll</subject><subject>Microbial Genetics and Genomics</subject><subject>Mutants</subject><subject>Necrosis</subject><subject>Original Article</subject><subject>Photosynthesis</subject><subject>Plant Genetics and Genomics</subject><subject>Reactive oxygen species</subject><subject>Salicylic acid</subject><subject>Transcriptomes</subject><subject>Triticum aestivum</subject><issn>1617-4615</issn><issn>1617-4623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp90E1Lw0AQBuBFFKzVP-BpwYsg0Z3ZTbI5llo_QFBQz8s2mbQpyaZmE4r99aaNKHjwtHN43mHnZewcxDUIEd94IZTUgUAIBGiAYHvARhBBHKgI5eHPDOExO_F-JQTEEcYjls3c0rqUMv464dZlfGrxivti4WxZuAVvyHdl63nh-Mv0NqgoK2zba7-uXWsd1Z3nJdmcO0qb2hd7uVmSbXnV9aDlG1-esqPclp7Ovt8xe7-bvU0fgqfn-8fp5ClIUWMbSJJaAymBKiUlc5QZiEinNkQinCdhaFOVKIjFnGQoQq3mYK0mzCTqJAU5ZpfD3nVTf3TkW1MVPqWyHD5qMIwUyAgQe3rxh67qrumP3ikdgY4xUb3CQe1u8w3lZt0UlW0-DQizK94MxZu-eLMv3mz7kBxCvsduQc3v6n9SX-guhOw</recordid><startdate>20211101</startdate><enddate>20211101</enddate><creator>Li, Huijuan</creator><creator>Jiao, Zhixin</creator><creator>Zhang, Peipei</creator><creator>Ni, Yongjing</creator><creator>Wang, Ting</creator><creator>Zhang, Jing</creator><creator>Li, Junchang</creator><creator>Jiang, Yumei</creator><creator>Yang, Xiwen</creator><creator>Li, Lei</creator><creator>Yao, Ziping</creator><creator>Niu, Jishan</creator><creator>He, Dexian</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7233-0442</orcidid></search><sort><creationdate>20211101</creationdate><title>Enhanced SA and Ca2+ signaling results in PCD-mediated spontaneous leaf necrosis in wheat mutant wsl</title><author>Li, Huijuan ; Jiao, Zhixin ; Zhang, Peipei ; Ni, Yongjing ; Wang, Ting ; Zhang, Jing ; Li, Junchang ; Jiang, Yumei ; Yang, Xiwen ; Li, Lei ; Yao, Ziping ; Niu, Jishan ; He, Dexian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-3e3881e4024ce43f23d1068ca52ee2b955ac494170be350584b1aa8e2d3289c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animal Genetics and Genomics</topic><topic>Apoptosis</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Calcium signalling</topic><topic>Cell death</topic><topic>Chlorophyll</topic><topic>Cultivars</topic><topic>Human Genetics</topic><topic>Leaf rust</topic><topic>Life Sciences</topic><topic>Mesophyll</topic><topic>Microbial Genetics and Genomics</topic><topic>Mutants</topic><topic>Necrosis</topic><topic>Original Article</topic><topic>Photosynthesis</topic><topic>Plant Genetics and Genomics</topic><topic>Reactive oxygen species</topic><topic>Salicylic acid</topic><topic>Transcriptomes</topic><topic>Triticum aestivum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Huijuan</creatorcontrib><creatorcontrib>Jiao, Zhixin</creatorcontrib><creatorcontrib>Zhang, Peipei</creatorcontrib><creatorcontrib>Ni, Yongjing</creatorcontrib><creatorcontrib>Wang, Ting</creatorcontrib><creatorcontrib>Zhang, Jing</creatorcontrib><creatorcontrib>Li, Junchang</creatorcontrib><creatorcontrib>Jiang, Yumei</creatorcontrib><creatorcontrib>Yang, Xiwen</creatorcontrib><creatorcontrib>Li, Lei</creatorcontrib><creatorcontrib>Yao, Ziping</creatorcontrib><creatorcontrib>Niu, Jishan</creatorcontrib><creatorcontrib>He, Dexian</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full 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Genomics</stitle><date>2021-11-01</date><risdate>2021</risdate><volume>296</volume><issue>6</issue><spage>1249</spage><epage>1262</epage><pages>1249-1262</pages><issn>1617-4615</issn><eissn>1617-4623</eissn><abstract>Leaf is the major photosynthesis organ and the key source of wheat (
Triticum aestivum
L.) grain. Spotted leaf (
spl
) mutant is a kind of leaf lesion mimic mutants (LMMs) in plants, which is an ideal material for studying the mechanisms of leaf development. In this study, we report the leaf abnormal development molecular mechanism of a
spl
mutant named
white stripe leaf
(
wsl
) derived from wheat cultivar Guomai 301 (WT). Histochemical observation indicated that the leaf mesophyll cells of the
wsl
were destroyed in the necrosis regions. To explore the molecular regulatory network of the leaf development in mutant
wsl
, we employed transcriptome analysis, histochemistry, quantitative real-time PCR (qRT-PCR), and observations of the key metabolites and photosynthesis parameters. Compared to WT, the expressions of the chlorophyll synthesis and photosynthesis-related homeotic genes were repressed; many genes in the WRKY transcription factor (TF) families were highly expressed; the salicylic acid (SA) and Ca
2+
signal transductions were enhanced in
wsl
. Both the chlorophyll contents and the photosynthesis rate were lower in
wsl
. The contents of SA and reactive oxygen species (ROS) were significantly higher, and the leaf rust resistance was enhanced in
wsl
. Based on the experimental data, a primary molecular regulatory model for leaf development in
wsl
was established. The results indicated that the SA accumulation and enhanced Ca
2+
signaling led to programmed cell death (PCD), and ultimately resulted in spontaneous leaf necrosis of
wsl.
These results laid a solid foundation for further research on the molecular mechanism of leaf development in wheat.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00438-021-01811-z</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-7233-0442</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Molecular genetics and genomics : MGG, 2021-11, Vol.296 (6), p.1249-1262 |
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| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Animal Genetics and Genomics Apoptosis Biochemistry Biomedical and Life Sciences Calcium signalling Cell death Chlorophyll Cultivars Human Genetics Leaf rust Life Sciences Mesophyll Microbial Genetics and Genomics Mutants Necrosis Original Article Photosynthesis Plant Genetics and Genomics Reactive oxygen species Salicylic acid Transcriptomes Triticum aestivum |
| title | Enhanced SA and Ca2+ signaling results in PCD-mediated spontaneous leaf necrosis in wheat mutant wsl |
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