Exfoliated bentonite/alginate nanocomposite hydrogel enhances intestinal delivery of probiotics by resistance to gastric pH and on-demand disintegration
In this study, we developed Lactobacillus rhamnosus GG (LGG)-encapsulating exfoliated bentonite/alginate nanocomposite hydrogels for protecting probiotics by delaying gastric fluid penetration into the nanocomposite and their on-demand release in the intestine. The pore size of the bentonite/alginat...
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| Veröffentlicht in: | Carbohydrate polymers 2021-11, Vol.272, p.118462-118462, Article 118462 |
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| container_title | Carbohydrate polymers |
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| creator | Kim, Jihyun Hlaing, Shwe Phyu Lee, Juho Saparbayeva, Aruzhan Kim, Sangsik Hwang, Dong Soo Lee, Eun Hee Yoon, In-Soo Yun, Hwayoung Kim, Min-Soo Moon, Hyung Ryong Jung, Yunjin Yoo, Jin-Wook |
| description | In this study, we developed Lactobacillus rhamnosus GG (LGG)-encapsulating exfoliated bentonite/alginate nanocomposite hydrogels for protecting probiotics by delaying gastric fluid penetration into the nanocomposite and their on-demand release in the intestine. The pore size of the bentonite/alginate nanocomposite hydrogels (BA15) was two-fold smaller than that of alginate hydrogel (BA00). Following gastric pH challenge, the survival of LGG in BA15 decreased by only 1.43 log CFU/g as compared to the 6.25 log CFU/g decrease in alginate (BA00). Further, the internal pH of BA15 decreased more gradually than that of BA00. After oral administration in mice, BA15 maintained shape integrity during gastric passage, followed by appropriate disintegration within the target intestinal area. Additionally, a fecal recovery experiment in mice showed that the viable counts of LGG in BA15 were six-fold higher than those in BA00. The findings suggest the exfoliated bentonite/alginate nanocomposite hydrogel as a promising platform for intestinal delivery of probiotics.
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•LGG was encapsulated in exfoliated bentonite/alginate nanocomposite hydrogels.•Improved hydrogel pore size dramatically enhanced LGG survival at gastric pH.•Complete intestinal release of LGG was observed after hydrogel disintegration.•Fecal recovery of bentonite/alginate LGG was 6-fold greater than of alginate LGG. |
| doi_str_mv | 10.1016/j.carbpol.2021.118462 |
| format | Article |
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[Display omitted]
•LGG was encapsulated in exfoliated bentonite/alginate nanocomposite hydrogels.•Improved hydrogel pore size dramatically enhanced LGG survival at gastric pH.•Complete intestinal release of LGG was observed after hydrogel disintegration.•Fecal recovery of bentonite/alginate LGG was 6-fold greater than of alginate LGG.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2021.118462</identifier><identifier>PMID: 34420722</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Administration, Oral ; Alginate ; Alginates - chemistry ; Animals ; Bentonite ; Bentonite - chemistry ; Feces - microbiology ; Gastric pH resistance ; Hydrogels - chemistry ; Hydrogen-Ion Concentration ; Intestinal delivery ; Intestines - metabolism ; Lactobacillus rhamnosus ; Male ; Mice ; Mice, Inbred ICR ; Microbial Viability ; Nanocomposite ; Nanocomposites - chemistry ; Probiotics ; Probiotics - administration & dosage ; Probiotics - chemistry ; Spectroscopy, Fourier Transform Infrared - methods ; X-Ray Diffraction - methods</subject><ispartof>Carbohydrate polymers, 2021-11, Vol.272, p.118462-118462, Article 118462</ispartof><rights>2021 Elsevier Ltd</rights><rights>Copyright © 2021 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-bebb57932143e2526d94ec82031923c7c33c44403fc59a05c258eae46af6152e3</citedby><cites>FETCH-LOGICAL-c365t-bebb57932143e2526d94ec82031923c7c33c44403fc59a05c258eae46af6152e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.carbpol.2021.118462$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34420722$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Jihyun</creatorcontrib><creatorcontrib>Hlaing, Shwe Phyu</creatorcontrib><creatorcontrib>Lee, Juho</creatorcontrib><creatorcontrib>Saparbayeva, Aruzhan</creatorcontrib><creatorcontrib>Kim, Sangsik</creatorcontrib><creatorcontrib>Hwang, Dong Soo</creatorcontrib><creatorcontrib>Lee, Eun Hee</creatorcontrib><creatorcontrib>Yoon, In-Soo</creatorcontrib><creatorcontrib>Yun, Hwayoung</creatorcontrib><creatorcontrib>Kim, Min-Soo</creatorcontrib><creatorcontrib>Moon, Hyung Ryong</creatorcontrib><creatorcontrib>Jung, Yunjin</creatorcontrib><creatorcontrib>Yoo, Jin-Wook</creatorcontrib><title>Exfoliated bentonite/alginate nanocomposite hydrogel enhances intestinal delivery of probiotics by resistance to gastric pH and on-demand disintegration</title><title>Carbohydrate polymers</title><addtitle>Carbohydr Polym</addtitle><description>In this study, we developed Lactobacillus rhamnosus GG (LGG)-encapsulating exfoliated bentonite/alginate nanocomposite hydrogels for protecting probiotics by delaying gastric fluid penetration into the nanocomposite and their on-demand release in the intestine. The pore size of the bentonite/alginate nanocomposite hydrogels (BA15) was two-fold smaller than that of alginate hydrogel (BA00). Following gastric pH challenge, the survival of LGG in BA15 decreased by only 1.43 log CFU/g as compared to the 6.25 log CFU/g decrease in alginate (BA00). Further, the internal pH of BA15 decreased more gradually than that of BA00. After oral administration in mice, BA15 maintained shape integrity during gastric passage, followed by appropriate disintegration within the target intestinal area. Additionally, a fecal recovery experiment in mice showed that the viable counts of LGG in BA15 were six-fold higher than those in BA00. The findings suggest the exfoliated bentonite/alginate nanocomposite hydrogel as a promising platform for intestinal delivery of probiotics.
[Display omitted]
•LGG was encapsulated in exfoliated bentonite/alginate nanocomposite hydrogels.•Improved hydrogel pore size dramatically enhanced LGG survival at gastric pH.•Complete intestinal release of LGG was observed after hydrogel disintegration.•Fecal recovery of bentonite/alginate LGG was 6-fold greater than of alginate LGG.</description><subject>Administration, Oral</subject><subject>Alginate</subject><subject>Alginates - chemistry</subject><subject>Animals</subject><subject>Bentonite</subject><subject>Bentonite - chemistry</subject><subject>Feces - microbiology</subject><subject>Gastric pH resistance</subject><subject>Hydrogels - chemistry</subject><subject>Hydrogen-Ion Concentration</subject><subject>Intestinal delivery</subject><subject>Intestines - metabolism</subject><subject>Lactobacillus rhamnosus</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Microbial Viability</subject><subject>Nanocomposite</subject><subject>Nanocomposites - chemistry</subject><subject>Probiotics</subject><subject>Probiotics - administration & dosage</subject><subject>Probiotics - chemistry</subject><subject>Spectroscopy, Fourier Transform Infrared - methods</subject><subject>X-Ray Diffraction - methods</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi1ERYfCI4C8ZJOpb3GSFUJVS5EqsSlry7FPph45drA9FfMmfdw6moEt3tg6_v9z-xD6RMmWEiqv91uj07hEv2WE0S2lvZDsDdrQvhsayoV4izaECtH0knaX6H3Oe1KPpOQduqzfjHSMbdDL7Z8peqcLWDxCKDG4Atfa71yoMRx0iCbOS8w1jJ-ONsUdeAzhSQcDGbtQIJeq9diCd8-QjjhOeElxdLE4k_F4xAmyy2U14BLxTueSnMHLPdbB4hgaC_P6si6v6XZJFxfDB3QxaZ_h4_m-Qr_ubh9v7puHn99_3Hx7aAyXbWlGGMe2GzijggNrmbSDANMzwunAuOkM50YIQfhk2kGT1rC2Bw1C6knSlgG_Ql9OeWvPvw91GDW7bMB7HSAesmKt5F3dleyrtD1JTYo5J5jUktys01FRolYoaq_OUNQKRZ2gVN_nc4nDOIP95_pLoQq-ngRQB312kFQ2Duq-rEtgirLR_afEK3Gyo-w</recordid><startdate>20211115</startdate><enddate>20211115</enddate><creator>Kim, Jihyun</creator><creator>Hlaing, Shwe Phyu</creator><creator>Lee, Juho</creator><creator>Saparbayeva, Aruzhan</creator><creator>Kim, Sangsik</creator><creator>Hwang, Dong Soo</creator><creator>Lee, Eun Hee</creator><creator>Yoon, In-Soo</creator><creator>Yun, Hwayoung</creator><creator>Kim, Min-Soo</creator><creator>Moon, Hyung Ryong</creator><creator>Jung, Yunjin</creator><creator>Yoo, Jin-Wook</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20211115</creationdate><title>Exfoliated bentonite/alginate nanocomposite hydrogel enhances intestinal delivery of probiotics by resistance to gastric pH and on-demand disintegration</title><author>Kim, Jihyun ; Hlaing, Shwe Phyu ; Lee, Juho ; Saparbayeva, Aruzhan ; Kim, Sangsik ; Hwang, Dong Soo ; Lee, Eun Hee ; Yoon, In-Soo ; Yun, Hwayoung ; Kim, Min-Soo ; Moon, Hyung Ryong ; Jung, Yunjin ; Yoo, Jin-Wook</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-bebb57932143e2526d94ec82031923c7c33c44403fc59a05c258eae46af6152e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Administration, Oral</topic><topic>Alginate</topic><topic>Alginates - chemistry</topic><topic>Animals</topic><topic>Bentonite</topic><topic>Bentonite - chemistry</topic><topic>Feces - microbiology</topic><topic>Gastric pH resistance</topic><topic>Hydrogels - chemistry</topic><topic>Hydrogen-Ion Concentration</topic><topic>Intestinal delivery</topic><topic>Intestines - metabolism</topic><topic>Lactobacillus rhamnosus</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Microbial Viability</topic><topic>Nanocomposite</topic><topic>Nanocomposites - chemistry</topic><topic>Probiotics</topic><topic>Probiotics - administration & dosage</topic><topic>Probiotics - chemistry</topic><topic>Spectroscopy, Fourier Transform Infrared - methods</topic><topic>X-Ray Diffraction - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Jihyun</creatorcontrib><creatorcontrib>Hlaing, Shwe Phyu</creatorcontrib><creatorcontrib>Lee, Juho</creatorcontrib><creatorcontrib>Saparbayeva, Aruzhan</creatorcontrib><creatorcontrib>Kim, Sangsik</creatorcontrib><creatorcontrib>Hwang, Dong Soo</creatorcontrib><creatorcontrib>Lee, Eun Hee</creatorcontrib><creatorcontrib>Yoon, In-Soo</creatorcontrib><creatorcontrib>Yun, Hwayoung</creatorcontrib><creatorcontrib>Kim, Min-Soo</creatorcontrib><creatorcontrib>Moon, Hyung Ryong</creatorcontrib><creatorcontrib>Jung, Yunjin</creatorcontrib><creatorcontrib>Yoo, Jin-Wook</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Jihyun</au><au>Hlaing, Shwe Phyu</au><au>Lee, Juho</au><au>Saparbayeva, Aruzhan</au><au>Kim, Sangsik</au><au>Hwang, Dong Soo</au><au>Lee, Eun Hee</au><au>Yoon, In-Soo</au><au>Yun, Hwayoung</au><au>Kim, Min-Soo</au><au>Moon, Hyung Ryong</au><au>Jung, Yunjin</au><au>Yoo, Jin-Wook</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exfoliated bentonite/alginate nanocomposite hydrogel enhances intestinal delivery of probiotics by resistance to gastric pH and on-demand disintegration</atitle><jtitle>Carbohydrate polymers</jtitle><addtitle>Carbohydr Polym</addtitle><date>2021-11-15</date><risdate>2021</risdate><volume>272</volume><spage>118462</spage><epage>118462</epage><pages>118462-118462</pages><artnum>118462</artnum><issn>0144-8617</issn><eissn>1879-1344</eissn><abstract>In this study, we developed Lactobacillus rhamnosus GG (LGG)-encapsulating exfoliated bentonite/alginate nanocomposite hydrogels for protecting probiotics by delaying gastric fluid penetration into the nanocomposite and their on-demand release in the intestine. The pore size of the bentonite/alginate nanocomposite hydrogels (BA15) was two-fold smaller than that of alginate hydrogel (BA00). Following gastric pH challenge, the survival of LGG in BA15 decreased by only 1.43 log CFU/g as compared to the 6.25 log CFU/g decrease in alginate (BA00). Further, the internal pH of BA15 decreased more gradually than that of BA00. After oral administration in mice, BA15 maintained shape integrity during gastric passage, followed by appropriate disintegration within the target intestinal area. Additionally, a fecal recovery experiment in mice showed that the viable counts of LGG in BA15 were six-fold higher than those in BA00. The findings suggest the exfoliated bentonite/alginate nanocomposite hydrogel as a promising platform for intestinal delivery of probiotics.
[Display omitted]
•LGG was encapsulated in exfoliated bentonite/alginate nanocomposite hydrogels.•Improved hydrogel pore size dramatically enhanced LGG survival at gastric pH.•Complete intestinal release of LGG was observed after hydrogel disintegration.•Fecal recovery of bentonite/alginate LGG was 6-fold greater than of alginate LGG.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>34420722</pmid><doi>10.1016/j.carbpol.2021.118462</doi><tpages>1</tpages></addata></record> |
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| ispartof | Carbohydrate polymers, 2021-11, Vol.272, p.118462-118462, Article 118462 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Administration, Oral Alginate Alginates - chemistry Animals Bentonite Bentonite - chemistry Feces - microbiology Gastric pH resistance Hydrogels - chemistry Hydrogen-Ion Concentration Intestinal delivery Intestines - metabolism Lactobacillus rhamnosus Male Mice Mice, Inbred ICR Microbial Viability Nanocomposite Nanocomposites - chemistry Probiotics Probiotics - administration & dosage Probiotics - chemistry Spectroscopy, Fourier Transform Infrared - methods X-Ray Diffraction - methods |
| title | Exfoliated bentonite/alginate nanocomposite hydrogel enhances intestinal delivery of probiotics by resistance to gastric pH and on-demand disintegration |
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