Automated segmentation and tracking of mitochondria in live-cell time-lapse images

Mitochondria display complex morphology and movements, which complicates their segmentation and tracking in time-lapse images. Here, we introduce Mitometer, an algorithm for fast, unbiased, and automated segmentation and tracking of mitochondria in live-cell two-dimensional and three-dimensional tim...

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Veröffentlicht in:	Nature methods 2021-09, Vol.18 (9), p.1091-1102
Hauptverfasser:	Lefebvre, Austin E. Y. T., Ma, Dennis, Kessenbrock, Kai, Lawson, Devon A., Digman, Michelle A.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/245/2225 631/1647/328 631/1647/794 631/80/2373 631/80/642 Algorithms Applications software Automation Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Breast cancer Breast Neoplasms - metabolism Breast Neoplasms - pathology Cell interaction Cell research Cells, Cultured Cytology Female Fission Fluorescence microscopy Humans Image processing Image segmentation Imaging, Three-Dimensional - methods Life Sciences Mammary Glands, Human - cytology Mechanical properties Methods Mitochondria Mitochondria - metabolism Molecular dynamics Morphology NAD - metabolism Physiological aspects Proteomics Reproducibility of Results Software Time-Lapse Imaging - methods Tracking Triple Negative Breast Neoplasms - pathology
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