Genetic relationships and biofilm formation of Listeria monocytogenes isolated from the smoked salmon industry
Among pathogens, L. monocytogenes has the capability to persist on Food Processing Environment (FPE), first of all posing safety issues, then economic impact on productivity. The aim of this work was to determine the influence of biofilm forming-ability and molecular features on the persistence of 1...
Gespeichert in:
| Veröffentlicht in: | International journal of food microbiology 2021-10, Vol.356, p.109353-109353, Article 109353 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 109353 |
|---|---|
| container_issue | |
| container_start_page | 109353 |
| container_title | International journal of food microbiology |
| container_volume | 356 |
| creator | Maggio, Francesca Rossi, Chiara Chiaverini, Alexandra Ruolo, Anna Orsini, Massimiliano Centorame, Patrizia Acciari, Vicdalia A. Chaves López, Clemencia Salini, Romolo Torresi, Marina Serio, Annalisa Pomilio, Francesco Paparella, Antonello |
| description | Among pathogens, L. monocytogenes has the capability to persist on Food Processing Environment (FPE), first of all posing safety issues, then economic impact on productivity. The aim of this work was to determine the influence of biofilm forming-ability and molecular features on the persistence of 19 Listeria monocytogenes isolates obtained from FPE, raw and processed products of a cold-smoked salmon processing plant. To verify the phenotypic and genomic correlations among the isolates, different analyses were employed: serotyping, Clonal Complex (CC), core genome Multi-Locus Sequence Typing (cgMLST) and Single Nucleotide Polymorphisms (SNPs) clustering, and evaluation of the presence of virulence- and persistence-associated genes. From our results, the biofilm formation was significantly higher (*P |
| doi_str_mv | 10.1016/j.ijfoodmicro.2021.109353 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2563426422</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168160521003123</els_id><sourcerecordid>2579412372</sourcerecordid><originalsourceid>FETCH-LOGICAL-c382t-92b023ec24a17ba23debefc5f5d9856ade597efe23629c4f1eb3c327cee049e3</originalsourceid><addsrcrecordid>eNqNkT1vGzEMhoUiBeqk-Q8qsnQ5Vx_3YY2F0aQBDHTJLugkKtH1TnQkuYD_feS4Q5ApE0HyeQmSLyHfOFtzxvsf0zpMHtEtwSZcCyZ4rSvZyU9kxTeDamTbswuyquym4T3rvpDLnCfGWCclW5F4BxFKsDTBbErAmJ_CPlMTHR0D-jAv1GNaXlsUPd2FXCAFQxeMaI8FH6s-05CxysFRn3Ch5QloXvBvzbOZK0lDdIdc0vEr-ezNnOH6f7wiD7e_Hra_m92fu_vtz11j5UaURomRCQlWtIYPoxHSwQjedr5zatP1xkGnBvAgZC-UbT2HUVopBgvAWgXyinw_j90nfD5ALnoJ2cI8mwh4yFp0vWxF3wpR0Zt36ISHFOtylRpUy4UcTpQ6U_XLOSfwep_CYtJRc6ZPRuhJvzFCn4zQZyOqdnvWQj34X4Cksw0QLbiQwBbtMHxgygsO35p8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2579412372</pqid></control><display><type>article</type><title>Genetic relationships and biofilm formation of Listeria monocytogenes isolated from the smoked salmon industry</title><source>Access via ScienceDirect (Elsevier)</source><creator>Maggio, Francesca ; Rossi, Chiara ; Chiaverini, Alexandra ; Ruolo, Anna ; Orsini, Massimiliano ; Centorame, Patrizia ; Acciari, Vicdalia A. ; Chaves López, Clemencia ; Salini, Romolo ; Torresi, Marina ; Serio, Annalisa ; Pomilio, Francesco ; Paparella, Antonello</creator><creatorcontrib>Maggio, Francesca ; Rossi, Chiara ; Chiaverini, Alexandra ; Ruolo, Anna ; Orsini, Massimiliano ; Centorame, Patrizia ; Acciari, Vicdalia A. ; Chaves López, Clemencia ; Salini, Romolo ; Torresi, Marina ; Serio, Annalisa ; Pomilio, Francesco ; Paparella, Antonello</creatorcontrib><description>Among pathogens, L. monocytogenes has the capability to persist on Food Processing Environment (FPE), first of all posing safety issues, then economic impact on productivity. The aim of this work was to determine the influence of biofilm forming-ability and molecular features on the persistence of 19 Listeria monocytogenes isolates obtained from FPE, raw and processed products of a cold-smoked salmon processing plant. To verify the phenotypic and genomic correlations among the isolates, different analyses were employed: serotyping, Clonal Complex (CC), core genome Multi-Locus Sequence Typing (cgMLST) and Single Nucleotide Polymorphisms (SNPs) clustering, and evaluation of the presence of virulence- and persistence-associated genes. From our results, the biofilm formation was significantly higher (*P < 0.05) at 37 °C, compared to 30 and 12 °C, suggesting a temperature-dependent behaviour. Moreover, the biofilm-forming ability showed a strain-specific trend, not correlated with CC or with strains persistence. Instead, the presence of internalin (inL), Stress Survival Islet (SSI) and resistance to erythromycin (ermC) genes was correlated with the ability to produce biofilms. Our data demonstrate that the genetic profile influences the adhesion capacity and persistence of L. monocytogenes in food processing plants and could be the result of environmental adaptation in response to the external selective pressure.
•19 L. monocytogenes isolates from a smoked salmon processing plant were analysed.•Persistence was related to biofilm-forming ability and genetic pattern.•Biofilm production is not the only requisite for isolates persistence.•inL, SSi and ermC correlated with ability to form biofilm.•Virulence and stress tolerance genes can determine persistence in L. monocytogenes.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2021.109353</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biofilm ; Biofilms ; Clustering ; Curing ; Economic impact ; Erythromycin ; External pressure ; Food plants ; Food processing ; Food processing plant ; Genes ; Genetic relationship ; Impact analysis ; Internalin ; L. monocytogenes ; Listeria ; Listeria monocytogenes ; Multilocus sequence typing ; Nucleotide sequence ; Nucleotides ; Persistence ; Salmon ; Serotyping ; Single-nucleotide polymorphism ; Temperature dependence ; Virulence ; WGS</subject><ispartof>International journal of food microbiology, 2021-10, Vol.356, p.109353-109353, Article 109353</ispartof><rights>2021 Elsevier B.V.</rights><rights>Copyright Elsevier BV Oct 16, 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-92b023ec24a17ba23debefc5f5d9856ade597efe23629c4f1eb3c327cee049e3</citedby><cites>FETCH-LOGICAL-c382t-92b023ec24a17ba23debefc5f5d9856ade597efe23629c4f1eb3c327cee049e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijfoodmicro.2021.109353$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Maggio, Francesca</creatorcontrib><creatorcontrib>Rossi, Chiara</creatorcontrib><creatorcontrib>Chiaverini, Alexandra</creatorcontrib><creatorcontrib>Ruolo, Anna</creatorcontrib><creatorcontrib>Orsini, Massimiliano</creatorcontrib><creatorcontrib>Centorame, Patrizia</creatorcontrib><creatorcontrib>Acciari, Vicdalia A.</creatorcontrib><creatorcontrib>Chaves López, Clemencia</creatorcontrib><creatorcontrib>Salini, Romolo</creatorcontrib><creatorcontrib>Torresi, Marina</creatorcontrib><creatorcontrib>Serio, Annalisa</creatorcontrib><creatorcontrib>Pomilio, Francesco</creatorcontrib><creatorcontrib>Paparella, Antonello</creatorcontrib><title>Genetic relationships and biofilm formation of Listeria monocytogenes isolated from the smoked salmon industry</title><title>International journal of food microbiology</title><description>Among pathogens, L. monocytogenes has the capability to persist on Food Processing Environment (FPE), first of all posing safety issues, then economic impact on productivity. The aim of this work was to determine the influence of biofilm forming-ability and molecular features on the persistence of 19 Listeria monocytogenes isolates obtained from FPE, raw and processed products of a cold-smoked salmon processing plant. To verify the phenotypic and genomic correlations among the isolates, different analyses were employed: serotyping, Clonal Complex (CC), core genome Multi-Locus Sequence Typing (cgMLST) and Single Nucleotide Polymorphisms (SNPs) clustering, and evaluation of the presence of virulence- and persistence-associated genes. From our results, the biofilm formation was significantly higher (*P < 0.05) at 37 °C, compared to 30 and 12 °C, suggesting a temperature-dependent behaviour. Moreover, the biofilm-forming ability showed a strain-specific trend, not correlated with CC or with strains persistence. Instead, the presence of internalin (inL), Stress Survival Islet (SSI) and resistance to erythromycin (ermC) genes was correlated with the ability to produce biofilms. Our data demonstrate that the genetic profile influences the adhesion capacity and persistence of L. monocytogenes in food processing plants and could be the result of environmental adaptation in response to the external selective pressure.
•19 L. monocytogenes isolates from a smoked salmon processing plant were analysed.•Persistence was related to biofilm-forming ability and genetic pattern.•Biofilm production is not the only requisite for isolates persistence.•inL, SSi and ermC correlated with ability to form biofilm.•Virulence and stress tolerance genes can determine persistence in L. monocytogenes.</description><subject>Biofilm</subject><subject>Biofilms</subject><subject>Clustering</subject><subject>Curing</subject><subject>Economic impact</subject><subject>Erythromycin</subject><subject>External pressure</subject><subject>Food plants</subject><subject>Food processing</subject><subject>Food processing plant</subject><subject>Genes</subject><subject>Genetic relationship</subject><subject>Impact analysis</subject><subject>Internalin</subject><subject>L. monocytogenes</subject><subject>Listeria</subject><subject>Listeria monocytogenes</subject><subject>Multilocus sequence typing</subject><subject>Nucleotide sequence</subject><subject>Nucleotides</subject><subject>Persistence</subject><subject>Salmon</subject><subject>Serotyping</subject><subject>Single-nucleotide polymorphism</subject><subject>Temperature dependence</subject><subject>Virulence</subject><subject>WGS</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqNkT1vGzEMhoUiBeqk-Q8qsnQ5Vx_3YY2F0aQBDHTJLugkKtH1TnQkuYD_feS4Q5ApE0HyeQmSLyHfOFtzxvsf0zpMHtEtwSZcCyZ4rSvZyU9kxTeDamTbswuyquym4T3rvpDLnCfGWCclW5F4BxFKsDTBbErAmJ_CPlMTHR0D-jAv1GNaXlsUPd2FXCAFQxeMaI8FH6s-05CxysFRn3Ch5QloXvBvzbOZK0lDdIdc0vEr-ezNnOH6f7wiD7e_Hra_m92fu_vtz11j5UaURomRCQlWtIYPoxHSwQjedr5zatP1xkGnBvAgZC-UbT2HUVopBgvAWgXyinw_j90nfD5ALnoJ2cI8mwh4yFp0vWxF3wpR0Zt36ISHFOtylRpUy4UcTpQ6U_XLOSfwep_CYtJRc6ZPRuhJvzFCn4zQZyOqdnvWQj34X4Cksw0QLbiQwBbtMHxgygsO35p8</recordid><startdate>20211016</startdate><enddate>20211016</enddate><creator>Maggio, Francesca</creator><creator>Rossi, Chiara</creator><creator>Chiaverini, Alexandra</creator><creator>Ruolo, Anna</creator><creator>Orsini, Massimiliano</creator><creator>Centorame, Patrizia</creator><creator>Acciari, Vicdalia A.</creator><creator>Chaves López, Clemencia</creator><creator>Salini, Romolo</creator><creator>Torresi, Marina</creator><creator>Serio, Annalisa</creator><creator>Pomilio, Francesco</creator><creator>Paparella, Antonello</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QR</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20211016</creationdate><title>Genetic relationships and biofilm formation of Listeria monocytogenes isolated from the smoked salmon industry</title><author>Maggio, Francesca ; Rossi, Chiara ; Chiaverini, Alexandra ; Ruolo, Anna ; Orsini, Massimiliano ; Centorame, Patrizia ; Acciari, Vicdalia A. ; Chaves López, Clemencia ; Salini, Romolo ; Torresi, Marina ; Serio, Annalisa ; Pomilio, Francesco ; Paparella, Antonello</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-92b023ec24a17ba23debefc5f5d9856ade597efe23629c4f1eb3c327cee049e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biofilm</topic><topic>Biofilms</topic><topic>Clustering</topic><topic>Curing</topic><topic>Economic impact</topic><topic>Erythromycin</topic><topic>External pressure</topic><topic>Food plants</topic><topic>Food processing</topic><topic>Food processing plant</topic><topic>Genes</topic><topic>Genetic relationship</topic><topic>Impact analysis</topic><topic>Internalin</topic><topic>L. monocytogenes</topic><topic>Listeria</topic><topic>Listeria monocytogenes</topic><topic>Multilocus sequence typing</topic><topic>Nucleotide sequence</topic><topic>Nucleotides</topic><topic>Persistence</topic><topic>Salmon</topic><topic>Serotyping</topic><topic>Single-nucleotide polymorphism</topic><topic>Temperature dependence</topic><topic>Virulence</topic><topic>WGS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maggio, Francesca</creatorcontrib><creatorcontrib>Rossi, Chiara</creatorcontrib><creatorcontrib>Chiaverini, Alexandra</creatorcontrib><creatorcontrib>Ruolo, Anna</creatorcontrib><creatorcontrib>Orsini, Massimiliano</creatorcontrib><creatorcontrib>Centorame, Patrizia</creatorcontrib><creatorcontrib>Acciari, Vicdalia A.</creatorcontrib><creatorcontrib>Chaves López, Clemencia</creatorcontrib><creatorcontrib>Salini, Romolo</creatorcontrib><creatorcontrib>Torresi, Marina</creatorcontrib><creatorcontrib>Serio, Annalisa</creatorcontrib><creatorcontrib>Pomilio, Francesco</creatorcontrib><creatorcontrib>Paparella, Antonello</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maggio, Francesca</au><au>Rossi, Chiara</au><au>Chiaverini, Alexandra</au><au>Ruolo, Anna</au><au>Orsini, Massimiliano</au><au>Centorame, Patrizia</au><au>Acciari, Vicdalia A.</au><au>Chaves López, Clemencia</au><au>Salini, Romolo</au><au>Torresi, Marina</au><au>Serio, Annalisa</au><au>Pomilio, Francesco</au><au>Paparella, Antonello</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic relationships and biofilm formation of Listeria monocytogenes isolated from the smoked salmon industry</atitle><jtitle>International journal of food microbiology</jtitle><date>2021-10-16</date><risdate>2021</risdate><volume>356</volume><spage>109353</spage><epage>109353</epage><pages>109353-109353</pages><artnum>109353</artnum><issn>0168-1605</issn><eissn>1879-3460</eissn><abstract>Among pathogens, L. monocytogenes has the capability to persist on Food Processing Environment (FPE), first of all posing safety issues, then economic impact on productivity. The aim of this work was to determine the influence of biofilm forming-ability and molecular features on the persistence of 19 Listeria monocytogenes isolates obtained from FPE, raw and processed products of a cold-smoked salmon processing plant. To verify the phenotypic and genomic correlations among the isolates, different analyses were employed: serotyping, Clonal Complex (CC), core genome Multi-Locus Sequence Typing (cgMLST) and Single Nucleotide Polymorphisms (SNPs) clustering, and evaluation of the presence of virulence- and persistence-associated genes. From our results, the biofilm formation was significantly higher (*P < 0.05) at 37 °C, compared to 30 and 12 °C, suggesting a temperature-dependent behaviour. Moreover, the biofilm-forming ability showed a strain-specific trend, not correlated with CC or with strains persistence. Instead, the presence of internalin (inL), Stress Survival Islet (SSI) and resistance to erythromycin (ermC) genes was correlated with the ability to produce biofilms. Our data demonstrate that the genetic profile influences the adhesion capacity and persistence of L. monocytogenes in food processing plants and could be the result of environmental adaptation in response to the external selective pressure.
•19 L. monocytogenes isolates from a smoked salmon processing plant were analysed.•Persistence was related to biofilm-forming ability and genetic pattern.•Biofilm production is not the only requisite for isolates persistence.•inL, SSi and ermC correlated with ability to form biofilm.•Virulence and stress tolerance genes can determine persistence in L. monocytogenes.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.ijfoodmicro.2021.109353</doi><tpages>1</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1605 |
| ispartof | International journal of food microbiology, 2021-10, Vol.356, p.109353-109353, Article 109353 |
| issn | 0168-1605 1879-3460 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2563426422 |
| source | Access via ScienceDirect (Elsevier) |
| subjects | Biofilm Biofilms Clustering Curing Economic impact Erythromycin External pressure Food plants Food processing Food processing plant Genes Genetic relationship Impact analysis Internalin L. monocytogenes Listeria Listeria monocytogenes Multilocus sequence typing Nucleotide sequence Nucleotides Persistence Salmon Serotyping Single-nucleotide polymorphism Temperature dependence Virulence WGS |
| title | Genetic relationships and biofilm formation of Listeria monocytogenes isolated from the smoked salmon industry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T10%3A58%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20relationships%20and%20biofilm%20formation%20of%20Listeria%20monocytogenes%20isolated%20from%20the%20smoked%20salmon%20industry&rft.jtitle=International%20journal%20of%20food%20microbiology&rft.au=Maggio,%20Francesca&rft.date=2021-10-16&rft.volume=356&rft.spage=109353&rft.epage=109353&rft.pages=109353-109353&rft.artnum=109353&rft.issn=0168-1605&rft.eissn=1879-3460&rft_id=info:doi/10.1016/j.ijfoodmicro.2021.109353&rft_dat=%3Cproquest_cross%3E2579412372%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2579412372&rft_id=info:pmid/&rft_els_id=S0168160521003123&rfr_iscdi=true |