Electrochemiluminescent determination of prostate-specific antigen using Au@(MoS2/GO/o-MWNTs) nanohybrids as co-reaction accelerator and hyperbranched hybridization chain reaction for signal amplification
Three-dimensional flowerlike Au@(MoS 2 /GO/o-MWNTs) nanohybrids (abbreviated as AMGMs) were synthesized and then introduced into an electrochemiluminescence (ECL) system as a new co-reaction accelerator for the ultrasensitive prostate-specific antigen (PSA). The AMGMs not only served as a substrate...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2021-09, Vol.188 (9), p.300-300, Article 300 |
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| creator | Sun, Yingying Qin, Yan Zhang, Jun Ren, Qunxiang |
| description | Three-dimensional flowerlike Au@(MoS
2
/GO/o-MWNTs) nanohybrids (abbreviated as AMGMs) were synthesized and then introduced into an electrochemiluminescence (ECL) system as a new co-reaction accelerator for the ultrasensitive prostate-specific antigen (PSA). The AMGMs not only served as a substrate with good conductivity and a large specific surface area for loading abundant primary antibodies but also acted as an effective co-reaction accelerator; the co-reaction accelerator could interact with a co-reactant rather than the luminophore to boost the generation of free radical intermediates, thereby producing abundant excited states of luminophores to amplify the ECL signal response. Additionally, an anticipated signal amplification strategy based on the hybridization chain reaction (HCR) was developed by gathering a large amount of a DNA initiator on gold nanoparticles. These gathered DNA initiators could generate multiple DNA concatemers and attach more signal molecules, which resulted in outstanding exponential signal amplification. Consequently, the ECL immunosensor demonstrated high sensitivity, with a linear range from 0.1 pg mL
−1
to 50 ng mL
−1
and a detection limit of 0.028 pg mL
−1
. In addition, the immunosensor displayed excellent stability and selectivity. It was evaluated by analyzing human serum sample. The recovery obtained was 98.80–112.00% and the RSD was 1.73–3.12%, indicating the immunosensor could be applied to the simultaneous detection of PSA in human serum samples.
Graphical abstract |
| doi_str_mv | 10.1007/s00604-021-04957-0 |
| format | Article |
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2
/GO/o-MWNTs) nanohybrids (abbreviated as AMGMs) were synthesized and then introduced into an electrochemiluminescence (ECL) system as a new co-reaction accelerator for the ultrasensitive prostate-specific antigen (PSA). The AMGMs not only served as a substrate with good conductivity and a large specific surface area for loading abundant primary antibodies but also acted as an effective co-reaction accelerator; the co-reaction accelerator could interact with a co-reactant rather than the luminophore to boost the generation of free radical intermediates, thereby producing abundant excited states of luminophores to amplify the ECL signal response. Additionally, an anticipated signal amplification strategy based on the hybridization chain reaction (HCR) was developed by gathering a large amount of a DNA initiator on gold nanoparticles. These gathered DNA initiators could generate multiple DNA concatemers and attach more signal molecules, which resulted in outstanding exponential signal amplification. Consequently, the ECL immunosensor demonstrated high sensitivity, with a linear range from 0.1 pg mL
−1
to 50 ng mL
−1
and a detection limit of 0.028 pg mL
−1
. In addition, the immunosensor displayed excellent stability and selectivity. It was evaluated by analyzing human serum sample. The recovery obtained was 98.80–112.00% and the RSD was 1.73–3.12%, indicating the immunosensor could be applied to the simultaneous detection of PSA in human serum samples.
Graphical abstract</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-021-04957-0</identifier><identifier>PMID: 34409505</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Amplification ; Analysis ; Analytical Chemistry ; Antibodies ; Antigen-antibody reactions ; Antigens ; Biosensing Techniques - methods ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Electric properties ; Electrochemical Techniques - methods ; Electrochemiluminescence ; Free radicals ; Humans ; Immunoassay - methods ; Immunosensors ; Initiators ; Male ; Microengineering ; Molybdenum disulfide ; Nanochemistry ; Nanoparticles ; Nanotechnology ; Original Paper ; Prostate ; Prostate-Specific Antigen - metabolism ; Selectivity ; Stability analysis ; Substrates ; Three dimensional flow</subject><ispartof>Mikrochimica acta (1966), 2021-09, Vol.188 (9), p.300-300, Article 300</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.</rights><rights>COPYRIGHT 2021 Springer</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-ff130311060e630aa6f1b262bfff9a5b5db9c6577aed726d68b8f72465b50f263</citedby><cites>FETCH-LOGICAL-c442t-ff130311060e630aa6f1b262bfff9a5b5db9c6577aed726d68b8f72465b50f263</cites><orcidid>0000-0003-0090-1602</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-021-04957-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-021-04957-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34409505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Yingying</creatorcontrib><creatorcontrib>Qin, Yan</creatorcontrib><creatorcontrib>Zhang, Jun</creatorcontrib><creatorcontrib>Ren, Qunxiang</creatorcontrib><title>Electrochemiluminescent determination of prostate-specific antigen using Au@(MoS2/GO/o-MWNTs) nanohybrids as co-reaction accelerator and hyperbranched hybridization chain reaction for signal amplification</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>Three-dimensional flowerlike Au@(MoS
2
/GO/o-MWNTs) nanohybrids (abbreviated as AMGMs) were synthesized and then introduced into an electrochemiluminescence (ECL) system as a new co-reaction accelerator for the ultrasensitive prostate-specific antigen (PSA). The AMGMs not only served as a substrate with good conductivity and a large specific surface area for loading abundant primary antibodies but also acted as an effective co-reaction accelerator; the co-reaction accelerator could interact with a co-reactant rather than the luminophore to boost the generation of free radical intermediates, thereby producing abundant excited states of luminophores to amplify the ECL signal response. Additionally, an anticipated signal amplification strategy based on the hybridization chain reaction (HCR) was developed by gathering a large amount of a DNA initiator on gold nanoparticles. These gathered DNA initiators could generate multiple DNA concatemers and attach more signal molecules, which resulted in outstanding exponential signal amplification. Consequently, the ECL immunosensor demonstrated high sensitivity, with a linear range from 0.1 pg mL
−1
to 50 ng mL
−1
and a detection limit of 0.028 pg mL
−1
. In addition, the immunosensor displayed excellent stability and selectivity. It was evaluated by analyzing human serum sample. The recovery obtained was 98.80–112.00% and the RSD was 1.73–3.12%, indicating the immunosensor could be applied to the simultaneous detection of PSA in human serum samples.
Graphical abstract</description><subject>Amplification</subject><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Antibodies</subject><subject>Antigen-antibody reactions</subject><subject>Antigens</subject><subject>Biosensing Techniques - methods</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Electric properties</subject><subject>Electrochemical Techniques - methods</subject><subject>Electrochemiluminescence</subject><subject>Free radicals</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Immunosensors</subject><subject>Initiators</subject><subject>Male</subject><subject>Microengineering</subject><subject>Molybdenum disulfide</subject><subject>Nanochemistry</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>Prostate</subject><subject>Prostate-Specific Antigen - metabolism</subject><subject>Selectivity</subject><subject>Stability analysis</subject><subject>Substrates</subject><subject>Three dimensional flow</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UsFu1DAUjBCILoUf4IAscSkHty-24-zeWFWlILX0QBFHy3Ged10l9mInh-Ub-SicTWkFQsgHy34z4_F7UxSvSzgtAeqzBCBBUGAlBbGqagpPikUpuKQV1PxpsQBgknJZs6PiRUp3AGUtmXheHHEhYFVBtSh-XnRohhjMFnvXjb3zmAz6gbQ4YMxHPbjgSbBkF0Ma9IA07dA46wzRfnAb9GRMzm_Ienx_ch2-sLPLm7NAr799vk3viNc-bPdNdG0iOhETaERtDpLaGOww6iHErNSS7X6HsYnaZyvTaSK5H_PzZqudJw9UmynJbbzuiO533WTmgHtZPLO6S_jqfj8uvn64uD3_SK9uLj-dr6-oEYIN1NqSAy_L3D2UHLSWtmyYZI21dqWrpmqblZFVXWtsayZbuWyWtmZC5hJYJvlxcTLr5p58HzENqne5a12nPYYxKVZJtuScA2To27-gd2GM2fmMqriAZfWI2ugOlfM2DFGbSVSt6zIPSi6FyKjTf6DyavPsTPBoXb7_g8BmgsmzSxGt2kXX67hXJagpQmqOkMoRUocIqcnxm3vHY9Nj-0D5nZkM4DMg5ZLfYHz80n9kfwEs4NRT</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Sun, Yingying</creator><creator>Qin, Yan</creator><creator>Zhang, Jun</creator><creator>Ren, Qunxiang</creator><general>Springer Vienna</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0090-1602</orcidid></search><sort><creationdate>20210901</creationdate><title>Electrochemiluminescent determination of prostate-specific antigen using Au@(MoS2/GO/o-MWNTs) nanohybrids as co-reaction accelerator and hyperbranched hybridization chain reaction for signal amplification</title><author>Sun, Yingying ; Qin, Yan ; Zhang, Jun ; Ren, Qunxiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-ff130311060e630aa6f1b262bfff9a5b5db9c6577aed726d68b8f72465b50f263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Amplification</topic><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Antibodies</topic><topic>Antigen-antibody reactions</topic><topic>Antigens</topic><topic>Biosensing Techniques - methods</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Electric properties</topic><topic>Electrochemical Techniques - methods</topic><topic>Electrochemiluminescence</topic><topic>Free radicals</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Immunosensors</topic><topic>Initiators</topic><topic>Male</topic><topic>Microengineering</topic><topic>Molybdenum disulfide</topic><topic>Nanochemistry</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>Prostate</topic><topic>Prostate-Specific Antigen - metabolism</topic><topic>Selectivity</topic><topic>Stability analysis</topic><topic>Substrates</topic><topic>Three dimensional flow</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Yingying</creatorcontrib><creatorcontrib>Qin, Yan</creatorcontrib><creatorcontrib>Zhang, Jun</creatorcontrib><creatorcontrib>Ren, Qunxiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Yingying</au><au>Qin, Yan</au><au>Zhang, Jun</au><au>Ren, Qunxiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemiluminescent determination of prostate-specific antigen using Au@(MoS2/GO/o-MWNTs) nanohybrids as co-reaction accelerator and hyperbranched hybridization chain reaction for signal amplification</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2021-09-01</date><risdate>2021</risdate><volume>188</volume><issue>9</issue><spage>300</spage><epage>300</epage><pages>300-300</pages><artnum>300</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>Three-dimensional flowerlike Au@(MoS
2
/GO/o-MWNTs) nanohybrids (abbreviated as AMGMs) were synthesized and then introduced into an electrochemiluminescence (ECL) system as a new co-reaction accelerator for the ultrasensitive prostate-specific antigen (PSA). The AMGMs not only served as a substrate with good conductivity and a large specific surface area for loading abundant primary antibodies but also acted as an effective co-reaction accelerator; the co-reaction accelerator could interact with a co-reactant rather than the luminophore to boost the generation of free radical intermediates, thereby producing abundant excited states of luminophores to amplify the ECL signal response. Additionally, an anticipated signal amplification strategy based on the hybridization chain reaction (HCR) was developed by gathering a large amount of a DNA initiator on gold nanoparticles. These gathered DNA initiators could generate multiple DNA concatemers and attach more signal molecules, which resulted in outstanding exponential signal amplification. Consequently, the ECL immunosensor demonstrated high sensitivity, with a linear range from 0.1 pg mL
−1
to 50 ng mL
−1
and a detection limit of 0.028 pg mL
−1
. In addition, the immunosensor displayed excellent stability and selectivity. It was evaluated by analyzing human serum sample. The recovery obtained was 98.80–112.00% and the RSD was 1.73–3.12%, indicating the immunosensor could be applied to the simultaneous detection of PSA in human serum samples.
Graphical abstract</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>34409505</pmid><doi>10.1007/s00604-021-04957-0</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-0090-1602</orcidid></addata></record> |
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| ispartof | Mikrochimica acta (1966), 2021-09, Vol.188 (9), p.300-300, Article 300 |
| issn | 0026-3672 1436-5073 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Amplification Analysis Analytical Chemistry Antibodies Antigen-antibody reactions Antigens Biosensing Techniques - methods Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Electric properties Electrochemical Techniques - methods Electrochemiluminescence Free radicals Humans Immunoassay - methods Immunosensors Initiators Male Microengineering Molybdenum disulfide Nanochemistry Nanoparticles Nanotechnology Original Paper Prostate Prostate-Specific Antigen - metabolism Selectivity Stability analysis Substrates Three dimensional flow |
| title | Electrochemiluminescent determination of prostate-specific antigen using Au@(MoS2/GO/o-MWNTs) nanohybrids as co-reaction accelerator and hyperbranched hybridization chain reaction for signal amplification |
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