Isolation and Characterization of Shiga Toxin-Producing Escherichia coli from Retail Beef Samples from Eight Provinces in China
While Shiga toxin-producing (STEC) is a major foodborne pathogen worldwide, data on the molecular and phylogenetic properties of STEC isolates from retail beef samples in China remain scant. Fresh retail beef samples ( = 1062) were collected from eight provinces, and STEC isolates were recovered an...
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| Veröffentlicht in: | Foodborne pathogens and disease 2021-08, Vol.18 (8), p.616-625 |
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| container_title | Foodborne pathogens and disease |
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| creator | Hu, Ying Cui, Guangqing Fan, Yiling Liu, Yan Zhou, Wei Huo, Shengnan Wu, Xin Song, Sheng Cui, Xuewen Zhao, Linna Bai, Li Cui, Shenghui He, Zhifei |
| description | While Shiga toxin-producing
(STEC) is a major foodborne pathogen worldwide, data on the molecular and phylogenetic properties of STEC isolates from retail beef samples in China remain scant. Fresh retail beef samples (
= 1062) were collected from eight provinces, and STEC isolates were recovered and characterized. PCR data showed that more than 50% of the samples were
positive, and 82 STEC isolates were recovered from 14.8% (79/535)
-positive enriched broths. In contrast, all ciprofloxacin resistant isolates (
= 19) and 13 cefotaxime (CTX) resistant isolates were
positive and belonged to three serotypes: O111:H8, O26:H11, or O157:H7. Point mutations in quinolone resistance-determining regions and plasmid-mediated quinolone resistance determinants were identified in 16 and 20 isolates, respectively.
and a point mutation (C-42T) in
promoter were detected in 15 and 8 of the CTX resistant isolates, respectively. In addition, macrolide resistance gene
was identified in eight azithromycin resistant O111:H8 isolates and one O26:H11 isolate. Single nucleotide polymorphism analysis demonstrated that the O26 and O157 isolates had multiple origins, but the O111 isolates were closely related. Taken together, our data demonstrated that several sequence types associated with hemolytic uremic syndrome from the retail beef samples in China had developed into dangerous multidrug resistant pathogens. The resistant phenotype can facilitate their transmission among the farm animals and human beings when there is an antimicrobial selective pressure. |
| doi_str_mv | 10.1089/fpd.2021.0005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2562520307</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2562520307</sourcerecordid><originalsourceid>FETCH-LOGICAL-c293t-2b807a786292324997414843ae4d58b2a05fb223199e5332ff14955812b003523</originalsourceid><addsrcrecordid>eNo9kMFOwzAMQCMEYmNw5Ipy5NKROMnaHGEaMAkJBONcpWm6BrXNSFoEXPh1Mm1wsmU_2_JD6JySKSWZvKo25RQI0CkhRBygMRVilqQUxOE2ZyJhlNMROgnhjRCQINJjNGKcEwYzOUY_y-Aa1VvXYdWVeF4rr3RvvP3eFV2FX2q7VnjlPm2XPHlXDtp2a7wIuo6Yrq3C2jUWV961-Nn0yjb4xpg4p9pNY8KusbDrusdx_MN2OhZtF2_ZTp2io0o1wZzt4wS93i5W8_vk4fFuOb9-SDRI1idQZCRVaTaLHzDgUqac8owzZXgpsgIUEVUBwKiURjAGVUW5FCKjUBDCBLAJutzt3Xj3PpjQ560N2jSN6owbQg5iBgIII2lEkx2qvQvBmyrfeNsq_5VTkm-d59F5vnWeb51H_mK_eihaU_7Tf5LZLxSJe8A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2562520307</pqid></control><display><type>article</type><title>Isolation and Characterization of Shiga Toxin-Producing Escherichia coli from Retail Beef Samples from Eight Provinces in China</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Hu, Ying ; Cui, Guangqing ; Fan, Yiling ; Liu, Yan ; Zhou, Wei ; Huo, Shengnan ; Wu, Xin ; Song, Sheng ; Cui, Xuewen ; Zhao, Linna ; Bai, Li ; Cui, Shenghui ; He, Zhifei</creator><creatorcontrib>Hu, Ying ; Cui, Guangqing ; Fan, Yiling ; Liu, Yan ; Zhou, Wei ; Huo, Shengnan ; Wu, Xin ; Song, Sheng ; Cui, Xuewen ; Zhao, Linna ; Bai, Li ; Cui, Shenghui ; He, Zhifei</creatorcontrib><description>While Shiga toxin-producing
(STEC) is a major foodborne pathogen worldwide, data on the molecular and phylogenetic properties of STEC isolates from retail beef samples in China remain scant. Fresh retail beef samples (
= 1062) were collected from eight provinces, and STEC isolates were recovered and characterized. PCR data showed that more than 50% of the samples were
positive, and 82 STEC isolates were recovered from 14.8% (79/535)
-positive enriched broths. In contrast, all ciprofloxacin resistant isolates (
= 19) and 13 cefotaxime (CTX) resistant isolates were
positive and belonged to three serotypes: O111:H8, O26:H11, or O157:H7. Point mutations in quinolone resistance-determining regions and plasmid-mediated quinolone resistance determinants were identified in 16 and 20 isolates, respectively.
and a point mutation (C-42T) in
promoter were detected in 15 and 8 of the CTX resistant isolates, respectively. In addition, macrolide resistance gene
was identified in eight azithromycin resistant O111:H8 isolates and one O26:H11 isolate. Single nucleotide polymorphism analysis demonstrated that the O26 and O157 isolates had multiple origins, but the O111 isolates were closely related. Taken together, our data demonstrated that several sequence types associated with hemolytic uremic syndrome from the retail beef samples in China had developed into dangerous multidrug resistant pathogens. The resistant phenotype can facilitate their transmission among the farm animals and human beings when there is an antimicrobial selective pressure.</description><identifier>ISSN: 1535-3141</identifier><identifier>EISSN: 1556-7125</identifier><identifier>DOI: 10.1089/fpd.2021.0005</identifier><identifier>PMID: 34403269</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; China ; Drug Resistance, Multiple - genetics ; Escherichia coli Proteins - isolation & purification ; Food Microbiology - statistics & numerical data ; Humans ; Phylogeny ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide ; Red Meat - microbiology ; Serogroup ; Shiga-Toxigenic Escherichia coli - genetics ; Shiga-Toxigenic Escherichia coli - isolation & purification ; Virulence Factors - genetics</subject><ispartof>Foodborne pathogens and disease, 2021-08, Vol.18 (8), p.616-625</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c293t-2b807a786292324997414843ae4d58b2a05fb223199e5332ff14955812b003523</citedby><cites>FETCH-LOGICAL-c293t-2b807a786292324997414843ae4d58b2a05fb223199e5332ff14955812b003523</cites><orcidid>0000-0001-9497-9253</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34403269$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Ying</creatorcontrib><creatorcontrib>Cui, Guangqing</creatorcontrib><creatorcontrib>Fan, Yiling</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Zhou, Wei</creatorcontrib><creatorcontrib>Huo, Shengnan</creatorcontrib><creatorcontrib>Wu, Xin</creatorcontrib><creatorcontrib>Song, Sheng</creatorcontrib><creatorcontrib>Cui, Xuewen</creatorcontrib><creatorcontrib>Zhao, Linna</creatorcontrib><creatorcontrib>Bai, Li</creatorcontrib><creatorcontrib>Cui, Shenghui</creatorcontrib><creatorcontrib>He, Zhifei</creatorcontrib><title>Isolation and Characterization of Shiga Toxin-Producing Escherichia coli from Retail Beef Samples from Eight Provinces in China</title><title>Foodborne pathogens and disease</title><addtitle>Foodborne Pathog Dis</addtitle><description>While Shiga toxin-producing
(STEC) is a major foodborne pathogen worldwide, data on the molecular and phylogenetic properties of STEC isolates from retail beef samples in China remain scant. Fresh retail beef samples (
= 1062) were collected from eight provinces, and STEC isolates were recovered and characterized. PCR data showed that more than 50% of the samples were
positive, and 82 STEC isolates were recovered from 14.8% (79/535)
-positive enriched broths. In contrast, all ciprofloxacin resistant isolates (
= 19) and 13 cefotaxime (CTX) resistant isolates were
positive and belonged to three serotypes: O111:H8, O26:H11, or O157:H7. Point mutations in quinolone resistance-determining regions and plasmid-mediated quinolone resistance determinants were identified in 16 and 20 isolates, respectively.
and a point mutation (C-42T) in
promoter were detected in 15 and 8 of the CTX resistant isolates, respectively. In addition, macrolide resistance gene
was identified in eight azithromycin resistant O111:H8 isolates and one O26:H11 isolate. Single nucleotide polymorphism analysis demonstrated that the O26 and O157 isolates had multiple origins, but the O111 isolates were closely related. Taken together, our data demonstrated that several sequence types associated with hemolytic uremic syndrome from the retail beef samples in China had developed into dangerous multidrug resistant pathogens. The resistant phenotype can facilitate their transmission among the farm animals and human beings when there is an antimicrobial selective pressure.</description><subject>Animals</subject><subject>China</subject><subject>Drug Resistance, Multiple - genetics</subject><subject>Escherichia coli Proteins - isolation & purification</subject><subject>Food Microbiology - statistics & numerical data</subject><subject>Humans</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Red Meat - microbiology</subject><subject>Serogroup</subject><subject>Shiga-Toxigenic Escherichia coli - genetics</subject><subject>Shiga-Toxigenic Escherichia coli - isolation & purification</subject><subject>Virulence Factors - genetics</subject><issn>1535-3141</issn><issn>1556-7125</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMFOwzAMQCMEYmNw5Ipy5NKROMnaHGEaMAkJBONcpWm6BrXNSFoEXPh1Mm1wsmU_2_JD6JySKSWZvKo25RQI0CkhRBygMRVilqQUxOE2ZyJhlNMROgnhjRCQINJjNGKcEwYzOUY_y-Aa1VvXYdWVeF4rr3RvvP3eFV2FX2q7VnjlPm2XPHlXDtp2a7wIuo6Yrq3C2jUWV961-Nn0yjb4xpg4p9pNY8KusbDrusdx_MN2OhZtF2_ZTp2io0o1wZzt4wS93i5W8_vk4fFuOb9-SDRI1idQZCRVaTaLHzDgUqac8owzZXgpsgIUEVUBwKiURjAGVUW5FCKjUBDCBLAJutzt3Xj3PpjQ560N2jSN6owbQg5iBgIII2lEkx2qvQvBmyrfeNsq_5VTkm-d59F5vnWeb51H_mK_eihaU_7Tf5LZLxSJe8A</recordid><startdate>202108</startdate><enddate>202108</enddate><creator>Hu, Ying</creator><creator>Cui, Guangqing</creator><creator>Fan, Yiling</creator><creator>Liu, Yan</creator><creator>Zhou, Wei</creator><creator>Huo, Shengnan</creator><creator>Wu, Xin</creator><creator>Song, Sheng</creator><creator>Cui, Xuewen</creator><creator>Zhao, Linna</creator><creator>Bai, Li</creator><creator>Cui, Shenghui</creator><creator>He, Zhifei</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9497-9253</orcidid></search><sort><creationdate>202108</creationdate><title>Isolation and Characterization of Shiga Toxin-Producing Escherichia coli from Retail Beef Samples from Eight Provinces in China</title><author>Hu, Ying ; Cui, Guangqing ; Fan, Yiling ; Liu, Yan ; Zhou, Wei ; Huo, Shengnan ; Wu, Xin ; Song, Sheng ; Cui, Xuewen ; Zhao, Linna ; Bai, Li ; Cui, Shenghui ; He, Zhifei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c293t-2b807a786292324997414843ae4d58b2a05fb223199e5332ff14955812b003523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>China</topic><topic>Drug Resistance, Multiple - genetics</topic><topic>Escherichia coli Proteins - isolation & purification</topic><topic>Food Microbiology - statistics & numerical data</topic><topic>Humans</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Red Meat - microbiology</topic><topic>Serogroup</topic><topic>Shiga-Toxigenic Escherichia coli - genetics</topic><topic>Shiga-Toxigenic Escherichia coli - isolation & purification</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Ying</creatorcontrib><creatorcontrib>Cui, Guangqing</creatorcontrib><creatorcontrib>Fan, Yiling</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Zhou, Wei</creatorcontrib><creatorcontrib>Huo, Shengnan</creatorcontrib><creatorcontrib>Wu, Xin</creatorcontrib><creatorcontrib>Song, Sheng</creatorcontrib><creatorcontrib>Cui, Xuewen</creatorcontrib><creatorcontrib>Zhao, Linna</creatorcontrib><creatorcontrib>Bai, Li</creatorcontrib><creatorcontrib>Cui, Shenghui</creatorcontrib><creatorcontrib>He, Zhifei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Foodborne pathogens and disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Ying</au><au>Cui, Guangqing</au><au>Fan, Yiling</au><au>Liu, Yan</au><au>Zhou, Wei</au><au>Huo, Shengnan</au><au>Wu, Xin</au><au>Song, Sheng</au><au>Cui, Xuewen</au><au>Zhao, Linna</au><au>Bai, Li</au><au>Cui, Shenghui</au><au>He, Zhifei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and Characterization of Shiga Toxin-Producing Escherichia coli from Retail Beef Samples from Eight Provinces in China</atitle><jtitle>Foodborne pathogens and disease</jtitle><addtitle>Foodborne Pathog Dis</addtitle><date>2021-08</date><risdate>2021</risdate><volume>18</volume><issue>8</issue><spage>616</spage><epage>625</epage><pages>616-625</pages><issn>1535-3141</issn><eissn>1556-7125</eissn><abstract>While Shiga toxin-producing
(STEC) is a major foodborne pathogen worldwide, data on the molecular and phylogenetic properties of STEC isolates from retail beef samples in China remain scant. Fresh retail beef samples (
= 1062) were collected from eight provinces, and STEC isolates were recovered and characterized. PCR data showed that more than 50% of the samples were
positive, and 82 STEC isolates were recovered from 14.8% (79/535)
-positive enriched broths. In contrast, all ciprofloxacin resistant isolates (
= 19) and 13 cefotaxime (CTX) resistant isolates were
positive and belonged to three serotypes: O111:H8, O26:H11, or O157:H7. Point mutations in quinolone resistance-determining regions and plasmid-mediated quinolone resistance determinants were identified in 16 and 20 isolates, respectively.
and a point mutation (C-42T) in
promoter were detected in 15 and 8 of the CTX resistant isolates, respectively. In addition, macrolide resistance gene
was identified in eight azithromycin resistant O111:H8 isolates and one O26:H11 isolate. Single nucleotide polymorphism analysis demonstrated that the O26 and O157 isolates had multiple origins, but the O111 isolates were closely related. Taken together, our data demonstrated that several sequence types associated with hemolytic uremic syndrome from the retail beef samples in China had developed into dangerous multidrug resistant pathogens. The resistant phenotype can facilitate their transmission among the farm animals and human beings when there is an antimicrobial selective pressure.</abstract><cop>United States</cop><pmid>34403269</pmid><doi>10.1089/fpd.2021.0005</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-9497-9253</orcidid></addata></record> |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals China Drug Resistance, Multiple - genetics Escherichia coli Proteins - isolation & purification Food Microbiology - statistics & numerical data Humans Phylogeny Polymerase Chain Reaction Polymorphism, Single Nucleotide Red Meat - microbiology Serogroup Shiga-Toxigenic Escherichia coli - genetics Shiga-Toxigenic Escherichia coli - isolation & purification Virulence Factors - genetics |
| title | Isolation and Characterization of Shiga Toxin-Producing Escherichia coli from Retail Beef Samples from Eight Provinces in China |
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