Bacterial strain for bast fiber crops degumming and its bio-degumming technique
The research and development of bio-degumming technology is under a slow progress due to the shortage of proper efficient bacterial strains and processes. A degumming bacterial strain— Pectobacterium wasabiae (PW)—with broad-spectrum degumming abilities was screened out in this study. After the ferm...
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| Veröffentlicht in: | Bioprocess and biosystems engineering 2021-12, Vol.44 (12), p.2503-2512 |
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| creator | Duan, Shengwen Xu, Bingrong Cheng, Lifeng Feng, Xiangyuan Yang, Qi Zheng, Ke Gao, Mingqiang Liu, Zhiyuan Liu, Chunjie Peng, Yuande |
| description | The research and development of bio-degumming technology is under a slow progress due to the shortage of proper efficient bacterial strains and processes. A degumming bacterial strain—
Pectobacterium wasabiae
(PW)—with broad-spectrum degumming abilities was screened out in this study. After the fermentation for 12 h, the residual gum contents of kenaf bast, ramie bast, hemp bast, flax bast, and Apocynum venetum bast were all lower than 15%. This bacterial strain could realize the simultaneous extracellular secretion of pectinase, mannase, and xylanase with the maximum enzyme activity levels of 130.25, 157.58, and 115.24 U/mL, respectively. The optimal degumming conditions of this bacterial strain were as follows: degumming time of 12 h, bath ratio of 1:10, temperature of 33 °C, and inoculum size of 2%. After the bio-degumming through this bacterial strain, the COD in wastewater was below 4000 mg/L, which was over 60% lower than that in boiling-off wastewater generated by chemical degumming. This technology achieves higher efficiency, higher quality, and lower pollution. |
| doi_str_mv | 10.1007/s00449-021-02622-7 |
| format | Article |
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Pectobacterium wasabiae
(PW)—with broad-spectrum degumming abilities was screened out in this study. After the fermentation for 12 h, the residual gum contents of kenaf bast, ramie bast, hemp bast, flax bast, and Apocynum venetum bast were all lower than 15%. This bacterial strain could realize the simultaneous extracellular secretion of pectinase, mannase, and xylanase with the maximum enzyme activity levels of 130.25, 157.58, and 115.24 U/mL, respectively. The optimal degumming conditions of this bacterial strain were as follows: degumming time of 12 h, bath ratio of 1:10, temperature of 33 °C, and inoculum size of 2%. After the bio-degumming through this bacterial strain, the COD in wastewater was below 4000 mg/L, which was over 60% lower than that in boiling-off wastewater generated by chemical degumming. This technology achieves higher efficiency, higher quality, and lower pollution.</description><identifier>ISSN: 1615-7591</identifier><identifier>EISSN: 1615-7605</identifier><identifier>DOI: 10.1007/s00449-021-02622-7</identifier><identifier>PMID: 34398326</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Bacteria ; Biotechnology ; Cellulose - metabolism ; Chemistry ; Chemistry and Materials Science ; Collaboration ; Crops ; Crops, Agricultural - metabolism ; Crops, Agricultural - microbiology ; Degumming ; Environmental Engineering/Biotechnology ; Enzymatic activity ; Enzyme activity ; Enzymes ; Fermentation ; Flax ; Food Science ; Genes, Bacterial ; Hemp ; Industrial and Production Engineering ; Industrial Chemistry/Chemical Engineering ; Inoculum ; Kenaf ; Moisture absorption ; Pectinase ; Pectobacterium - classification ; Pectobacterium - genetics ; Pectobacterium - metabolism ; Phylogeny ; R&D ; Raw materials ; Research & development ; Research Paper ; RNA, Ribosomal, 16S - genetics ; Vegetable fibers ; Wastewater ; Xylanase</subject><ispartof>Bioprocess and biosystems engineering, 2021-12, Vol.44 (12), p.2503-2512</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-87b9e69a0673cbe989368ef3dce1172396d3106f019c68ce13e6d2e9dc5fc25b3</citedby><cites>FETCH-LOGICAL-c375t-87b9e69a0673cbe989368ef3dce1172396d3106f019c68ce13e6d2e9dc5fc25b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00449-021-02622-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00449-021-02622-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34398326$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duan, Shengwen</creatorcontrib><creatorcontrib>Xu, Bingrong</creatorcontrib><creatorcontrib>Cheng, Lifeng</creatorcontrib><creatorcontrib>Feng, Xiangyuan</creatorcontrib><creatorcontrib>Yang, Qi</creatorcontrib><creatorcontrib>Zheng, Ke</creatorcontrib><creatorcontrib>Gao, Mingqiang</creatorcontrib><creatorcontrib>Liu, Zhiyuan</creatorcontrib><creatorcontrib>Liu, Chunjie</creatorcontrib><creatorcontrib>Peng, Yuande</creatorcontrib><title>Bacterial strain for bast fiber crops degumming and its bio-degumming technique</title><title>Bioprocess and biosystems engineering</title><addtitle>Bioprocess Biosyst Eng</addtitle><addtitle>Bioprocess Biosyst Eng</addtitle><description>The research and development of bio-degumming technology is under a slow progress due to the shortage of proper efficient bacterial strains and processes. A degumming bacterial strain—
Pectobacterium wasabiae
(PW)—with broad-spectrum degumming abilities was screened out in this study. After the fermentation for 12 h, the residual gum contents of kenaf bast, ramie bast, hemp bast, flax bast, and Apocynum venetum bast were all lower than 15%. This bacterial strain could realize the simultaneous extracellular secretion of pectinase, mannase, and xylanase with the maximum enzyme activity levels of 130.25, 157.58, and 115.24 U/mL, respectively. The optimal degumming conditions of this bacterial strain were as follows: degumming time of 12 h, bath ratio of 1:10, temperature of 33 °C, and inoculum size of 2%. After the bio-degumming through this bacterial strain, the COD in wastewater was below 4000 mg/L, which was over 60% lower than that in boiling-off wastewater generated by chemical degumming. This technology achieves higher efficiency, higher quality, and lower pollution.</description><subject>Bacteria</subject><subject>Biotechnology</subject><subject>Cellulose - metabolism</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Collaboration</subject><subject>Crops</subject><subject>Crops, Agricultural - metabolism</subject><subject>Crops, Agricultural - microbiology</subject><subject>Degumming</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>Fermentation</subject><subject>Flax</subject><subject>Food Science</subject><subject>Genes, Bacterial</subject><subject>Hemp</subject><subject>Industrial and Production Engineering</subject><subject>Industrial Chemistry/Chemical Engineering</subject><subject>Inoculum</subject><subject>Kenaf</subject><subject>Moisture absorption</subject><subject>Pectinase</subject><subject>Pectobacterium - classification</subject><subject>Pectobacterium - genetics</subject><subject>Pectobacterium - metabolism</subject><subject>Phylogeny</subject><subject>R&D</subject><subject>Raw materials</subject><subject>Research & development</subject><subject>Research Paper</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Vegetable fibers</subject><subject>Wastewater</subject><subject>Xylanase</subject><issn>1615-7591</issn><issn>1615-7605</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kLtOwzAUhi0EolB4AQZkiYUl4EtsxyNU3KRKXWC2HOekuGqSYicDb48hLZUYGCxbx9_5j_0hdEHJDSVE3UZC8lxnhNG0JGOZOkAnVFKRKUnE4e4sNJ2g0xhXhFBRMHKMJjznuuBMnqDFvXU9BG_XOPbB-hbXXcCljT2ufQkBu9BtIq5gOTSNb5fYthX2fcSl77J9tQf33vqPAc7QUW3XEc63-xS9PT68zp6z-eLpZXY3zxxXos8KVWqQ2hKpuCtBF5rLAmpeOaBUMa5lxSmRNaHaySIVOciKga6cqB0TJZ-i6zF3E7o0Nvam8dHBem1b6IZomJBUs5yoPKFXf9BVN4Q2vS5RRS7z5JIlio1U-nCMAWqzCb6x4dNQYr51m1G3SbrNj26jUtPlNnooG6h-W3Z-E8BHIKardglhP_uf2C9lvInh</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Duan, Shengwen</creator><creator>Xu, Bingrong</creator><creator>Cheng, Lifeng</creator><creator>Feng, Xiangyuan</creator><creator>Yang, Qi</creator><creator>Zheng, Ke</creator><creator>Gao, Mingqiang</creator><creator>Liu, Zhiyuan</creator><creator>Liu, Chunjie</creator><creator>Peng, Yuande</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20211201</creationdate><title>Bacterial strain for bast fiber crops degumming and its bio-degumming technique</title><author>Duan, Shengwen ; Xu, Bingrong ; Cheng, Lifeng ; Feng, Xiangyuan ; Yang, Qi ; Zheng, Ke ; Gao, Mingqiang ; Liu, Zhiyuan ; Liu, Chunjie ; Peng, Yuande</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-87b9e69a0673cbe989368ef3dce1172396d3106f019c68ce13e6d2e9dc5fc25b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Bacteria</topic><topic>Biotechnology</topic><topic>Cellulose - metabolism</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Collaboration</topic><topic>Crops</topic><topic>Crops, Agricultural - metabolism</topic><topic>Crops, Agricultural - microbiology</topic><topic>Degumming</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Enzymes</topic><topic>Fermentation</topic><topic>Flax</topic><topic>Food Science</topic><topic>Genes, Bacterial</topic><topic>Hemp</topic><topic>Industrial and Production Engineering</topic><topic>Industrial Chemistry/Chemical Engineering</topic><topic>Inoculum</topic><topic>Kenaf</topic><topic>Moisture absorption</topic><topic>Pectinase</topic><topic>Pectobacterium - classification</topic><topic>Pectobacterium - genetics</topic><topic>Pectobacterium - metabolism</topic><topic>Phylogeny</topic><topic>R&D</topic><topic>Raw materials</topic><topic>Research & development</topic><topic>Research Paper</topic><topic>RNA, Ribosomal, 16S - 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Academic</collection><jtitle>Bioprocess and biosystems engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duan, Shengwen</au><au>Xu, Bingrong</au><au>Cheng, Lifeng</au><au>Feng, Xiangyuan</au><au>Yang, Qi</au><au>Zheng, Ke</au><au>Gao, Mingqiang</au><au>Liu, Zhiyuan</au><au>Liu, Chunjie</au><au>Peng, Yuande</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial strain for bast fiber crops degumming and its bio-degumming technique</atitle><jtitle>Bioprocess and biosystems engineering</jtitle><stitle>Bioprocess Biosyst Eng</stitle><addtitle>Bioprocess Biosyst Eng</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>44</volume><issue>12</issue><spage>2503</spage><epage>2512</epage><pages>2503-2512</pages><issn>1615-7591</issn><eissn>1615-7605</eissn><abstract>The research and development of bio-degumming technology is under a slow progress due to the shortage of proper efficient bacterial strains and processes. A degumming bacterial strain—
Pectobacterium wasabiae
(PW)—with broad-spectrum degumming abilities was screened out in this study. After the fermentation for 12 h, the residual gum contents of kenaf bast, ramie bast, hemp bast, flax bast, and Apocynum venetum bast were all lower than 15%. This bacterial strain could realize the simultaneous extracellular secretion of pectinase, mannase, and xylanase with the maximum enzyme activity levels of 130.25, 157.58, and 115.24 U/mL, respectively. The optimal degumming conditions of this bacterial strain were as follows: degumming time of 12 h, bath ratio of 1:10, temperature of 33 °C, and inoculum size of 2%. After the bio-degumming through this bacterial strain, the COD in wastewater was below 4000 mg/L, which was over 60% lower than that in boiling-off wastewater generated by chemical degumming. This technology achieves higher efficiency, higher quality, and lower pollution.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>34398326</pmid><doi>10.1007/s00449-021-02622-7</doi><tpages>10</tpages></addata></record> |
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| subjects | Bacteria Biotechnology Cellulose - metabolism Chemistry Chemistry and Materials Science Collaboration Crops Crops, Agricultural - metabolism Crops, Agricultural - microbiology Degumming Environmental Engineering/Biotechnology Enzymatic activity Enzyme activity Enzymes Fermentation Flax Food Science Genes, Bacterial Hemp Industrial and Production Engineering Industrial Chemistry/Chemical Engineering Inoculum Kenaf Moisture absorption Pectinase Pectobacterium - classification Pectobacterium - genetics Pectobacterium - metabolism Phylogeny R&D Raw materials Research & development Research Paper RNA, Ribosomal, 16S - genetics Vegetable fibers Wastewater Xylanase |
| title | Bacterial strain for bast fiber crops degumming and its bio-degumming technique |
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