Gingival tissue as a reservoir for human immunodeficiency virus type 1: Preliminary results of a cross‐sectional observational study
Background Despite combined antiretroviral therapy (cART), total cure of immunodeficiency virus type 1 (HIV‐1) infection remains elusive. Chronic periodontitis (CP) is strongly associated with HIV‐1 infection. This condition is characterized by an intense inflammatory infiltrate mainly constituted o...
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| Veröffentlicht in: | Journal of periodontology (1970) 2022-04, Vol.93 (4), p.613-620 |
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| container_title | Journal of periodontology (1970) |
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| creator | Sereme, Youssouf Pólvora, Tábata Larissa S. Rochereau, Nicolas Teixeira, Lucas Ribeiro Santana, Rodrigo Carvalho Paul, Stephane Fonseca, Benedito Antonio Lopes Bourlet, Thomas Pozzetto, Bruno Lourenço, Alan Grupioni Motta, Ana Carolina F. |
| description | Background
Despite combined antiretroviral therapy (cART), total cure of immunodeficiency virus type 1 (HIV‐1) infection remains elusive. Chronic periodontitis (CP) is strongly associated with HIV‐1 infection. This condition is characterized by an intense inflammatory infiltrate mainly constituted of immune cells which in turn may be a valuable source of HIV‐1 reactivation. This study aimed to determine if gingival tissue could act as a reservoir for HIV‐1.
Methods
Twelve patients with HIV‐1 and CP and 12 controls (no HIV‐1‐infection and no CP) were evaluated in a cross‐sectional study. RNA viral load and interleukin (IL) levels were determined in blood plasma and saliva. Histological sections of gingival tissue were stained with fluorescent antibodies against p24 antigen and different cellular biomarkers.
Results
In six of the 12 patients, HIV RNA load was detected, despite cART; in three of them, expression of viral RNA was also detected in saliva. The levels of IL‐2, IL‐6, and IL‐12 were higher in blood and saliva of patients with HIVand CP than controls. HIV‐1 p24 antigen was detected by immunostaining in gingival biopsies of 10 of the 12 patients but in no controls. Immune markers for T cells and antigen‐presenting cells were also identified in most patients and some controls.
Conclusion
These preliminary data showing the detection of HIV‐1 p24 antigen in the gingival biopsies of a significant part of patients with HIV‐1 and CP under cART together with the presence of immune cells, plead for the existence of a HIV‐1 reservoir in the gingival tissue of this population. |
| doi_str_mv | 10.1002/JPER.21-0345 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_proquest_miscellaneous_2561911047</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2561911047</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3698-4694c1bb7f166d129c3495895e3ddc20c6d2a41dba2cf816eb091dafbf908953</originalsourceid><addsrcrecordid>eNp9kU9vFCEYh4mxsWv15tlw1MSpvMCwg7emqa3NJm1M74Thj8XMDCvMrJmbJ89-Rj-JTHftsSfyg4cH3vwQegPkFAihH69vL76eUqgI4_UztALJWcXEmjxHq3JMK8YlPUYvc_5eInBGXqBjxpkUNa1X6PdlGL6Fne7wGHKeHNYZa5xcdmkXQ8I-Jnw_9XrAoe-nIVrngwluMDPehTRlPM5bh-ETvk2uC30YdJqX61M3Zhx9cZkUc_776092ZgxxKC_FdrHrQ8rjZOdX6MjrLrvXh_UE3X2-uDu_qjY3l1_OzzaVYUI2FReSG2jbtQchLFBpynR1I2vHrDWUGGGp5mBbTY1vQLiWSLDat16SQrET9H6vvded2qbQl9-qqIO6OtuoZY_wZk2lgB0U9t2e3ab4Y3J5VH3IxnWdHlycsqK1AAlA-LqgH_bow6zJ-Uc3ELWUpJaSFAW1lFTwtwfz1PbOPsL_WykA2wM_Q-fmJ2UPAUjDG_YP2JyfKQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2561911047</pqid></control><display><type>article</type><title>Gingival tissue as a reservoir for human immunodeficiency virus type 1: Preliminary results of a cross‐sectional observational study</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Sereme, Youssouf ; Pólvora, Tábata Larissa S. ; Rochereau, Nicolas ; Teixeira, Lucas Ribeiro ; Santana, Rodrigo Carvalho ; Paul, Stephane ; Fonseca, Benedito Antonio Lopes ; Bourlet, Thomas ; Pozzetto, Bruno ; Lourenço, Alan Grupioni ; Motta, Ana Carolina F.</creator><creatorcontrib>Sereme, Youssouf ; Pólvora, Tábata Larissa S. ; Rochereau, Nicolas ; Teixeira, Lucas Ribeiro ; Santana, Rodrigo Carvalho ; Paul, Stephane ; Fonseca, Benedito Antonio Lopes ; Bourlet, Thomas ; Pozzetto, Bruno ; Lourenço, Alan Grupioni ; Motta, Ana Carolina F.</creatorcontrib><description>Background
Despite combined antiretroviral therapy (cART), total cure of immunodeficiency virus type 1 (HIV‐1) infection remains elusive. Chronic periodontitis (CP) is strongly associated with HIV‐1 infection. This condition is characterized by an intense inflammatory infiltrate mainly constituted of immune cells which in turn may be a valuable source of HIV‐1 reactivation. This study aimed to determine if gingival tissue could act as a reservoir for HIV‐1.
Methods
Twelve patients with HIV‐1 and CP and 12 controls (no HIV‐1‐infection and no CP) were evaluated in a cross‐sectional study. RNA viral load and interleukin (IL) levels were determined in blood plasma and saliva. Histological sections of gingival tissue were stained with fluorescent antibodies against p24 antigen and different cellular biomarkers.
Results
In six of the 12 patients, HIV RNA load was detected, despite cART; in three of them, expression of viral RNA was also detected in saliva. The levels of IL‐2, IL‐6, and IL‐12 were higher in blood and saliva of patients with HIVand CP than controls. HIV‐1 p24 antigen was detected by immunostaining in gingival biopsies of 10 of the 12 patients but in no controls. Immune markers for T cells and antigen‐presenting cells were also identified in most patients and some controls.
Conclusion
These preliminary data showing the detection of HIV‐1 p24 antigen in the gingival biopsies of a significant part of patients with HIV‐1 and CP under cART together with the presence of immune cells, plead for the existence of a HIV‐1 reservoir in the gingival tissue of this population.</description><identifier>ISSN: 0022-3492</identifier><identifier>EISSN: 1943-3670</identifier><identifier>DOI: 10.1002/JPER.21-0345</identifier><identifier>PMID: 34396525</identifier><language>eng</language><publisher>United States: American Academy of Periodontology</publisher><subject>Cross-Sectional Studies ; gingiva ; HIV Core Protein p24 ; HIV Infections - drug therapy ; HIV-1 - genetics ; HIV‐1 ; Human health and pathology ; Humans ; Immunology ; Infectious diseases ; inflammation ; Life Sciences ; Microbiology and Parasitology ; mouth ; mucous membrane ; periodontitis ; Pharmaceutical sciences ; Pharmacology ; RNA ; saliva ; Vaccinology ; Viral Load ; Virology</subject><ispartof>Journal of periodontology (1970), 2022-04, Vol.93 (4), p.613-620</ispartof><rights>2021 American Academy of Periodontology</rights><rights>2021 American Academy of Periodontology.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3698-4694c1bb7f166d129c3495895e3ddc20c6d2a41dba2cf816eb091dafbf908953</citedby><cites>FETCH-LOGICAL-c3698-4694c1bb7f166d129c3495895e3ddc20c6d2a41dba2cf816eb091dafbf908953</cites><orcidid>0000-0002-2603-8467 ; 0000-0002-8830-4273 ; 0000-0002-6522-895X ; 0000-0002-9226-1149 ; 0000-0002-3887-9239 ; 0000-0002-5887-8663 ; 0000-0002-5607-1460 ; 0000-0003-3159-5687 ; 0000-0002-4005-157X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2FJPER.21-0345$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2FJPER.21-0345$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,777,781,882,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34396525$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04872961$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Sereme, Youssouf</creatorcontrib><creatorcontrib>Pólvora, Tábata Larissa S.</creatorcontrib><creatorcontrib>Rochereau, Nicolas</creatorcontrib><creatorcontrib>Teixeira, Lucas Ribeiro</creatorcontrib><creatorcontrib>Santana, Rodrigo Carvalho</creatorcontrib><creatorcontrib>Paul, Stephane</creatorcontrib><creatorcontrib>Fonseca, Benedito Antonio Lopes</creatorcontrib><creatorcontrib>Bourlet, Thomas</creatorcontrib><creatorcontrib>Pozzetto, Bruno</creatorcontrib><creatorcontrib>Lourenço, Alan Grupioni</creatorcontrib><creatorcontrib>Motta, Ana Carolina F.</creatorcontrib><title>Gingival tissue as a reservoir for human immunodeficiency virus type 1: Preliminary results of a cross‐sectional observational study</title><title>Journal of periodontology (1970)</title><addtitle>J Periodontol</addtitle><description>Background
Despite combined antiretroviral therapy (cART), total cure of immunodeficiency virus type 1 (HIV‐1) infection remains elusive. Chronic periodontitis (CP) is strongly associated with HIV‐1 infection. This condition is characterized by an intense inflammatory infiltrate mainly constituted of immune cells which in turn may be a valuable source of HIV‐1 reactivation. This study aimed to determine if gingival tissue could act as a reservoir for HIV‐1.
Methods
Twelve patients with HIV‐1 and CP and 12 controls (no HIV‐1‐infection and no CP) were evaluated in a cross‐sectional study. RNA viral load and interleukin (IL) levels were determined in blood plasma and saliva. Histological sections of gingival tissue were stained with fluorescent antibodies against p24 antigen and different cellular biomarkers.
Results
In six of the 12 patients, HIV RNA load was detected, despite cART; in three of them, expression of viral RNA was also detected in saliva. The levels of IL‐2, IL‐6, and IL‐12 were higher in blood and saliva of patients with HIVand CP than controls. HIV‐1 p24 antigen was detected by immunostaining in gingival biopsies of 10 of the 12 patients but in no controls. Immune markers for T cells and antigen‐presenting cells were also identified in most patients and some controls.
Conclusion
These preliminary data showing the detection of HIV‐1 p24 antigen in the gingival biopsies of a significant part of patients with HIV‐1 and CP under cART together with the presence of immune cells, plead for the existence of a HIV‐1 reservoir in the gingival tissue of this population.</description><subject>Cross-Sectional Studies</subject><subject>gingiva</subject><subject>HIV Core Protein p24</subject><subject>HIV Infections - drug therapy</subject><subject>HIV-1 - genetics</subject><subject>HIV‐1</subject><subject>Human health and pathology</subject><subject>Humans</subject><subject>Immunology</subject><subject>Infectious diseases</subject><subject>inflammation</subject><subject>Life Sciences</subject><subject>Microbiology and Parasitology</subject><subject>mouth</subject><subject>mucous membrane</subject><subject>periodontitis</subject><subject>Pharmaceutical sciences</subject><subject>Pharmacology</subject><subject>RNA</subject><subject>saliva</subject><subject>Vaccinology</subject><subject>Viral Load</subject><subject>Virology</subject><issn>0022-3492</issn><issn>1943-3670</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9vFCEYh4mxsWv15tlw1MSpvMCwg7emqa3NJm1M74Thj8XMDCvMrJmbJ89-Rj-JTHftsSfyg4cH3vwQegPkFAihH69vL76eUqgI4_UztALJWcXEmjxHq3JMK8YlPUYvc_5eInBGXqBjxpkUNa1X6PdlGL6Fne7wGHKeHNYZa5xcdmkXQ8I-Jnw_9XrAoe-nIVrngwluMDPehTRlPM5bh-ETvk2uC30YdJqX61M3Zhx9cZkUc_776092ZgxxKC_FdrHrQ8rjZOdX6MjrLrvXh_UE3X2-uDu_qjY3l1_OzzaVYUI2FReSG2jbtQchLFBpynR1I2vHrDWUGGGp5mBbTY1vQLiWSLDat16SQrET9H6vvded2qbQl9-qqIO6OtuoZY_wZk2lgB0U9t2e3ab4Y3J5VH3IxnWdHlycsqK1AAlA-LqgH_bow6zJ-Uc3ELWUpJaSFAW1lFTwtwfz1PbOPsL_WykA2wM_Q-fmJ2UPAUjDG_YP2JyfKQ</recordid><startdate>202204</startdate><enddate>202204</enddate><creator>Sereme, Youssouf</creator><creator>Pólvora, Tábata Larissa S.</creator><creator>Rochereau, Nicolas</creator><creator>Teixeira, Lucas Ribeiro</creator><creator>Santana, Rodrigo Carvalho</creator><creator>Paul, Stephane</creator><creator>Fonseca, Benedito Antonio Lopes</creator><creator>Bourlet, Thomas</creator><creator>Pozzetto, Bruno</creator><creator>Lourenço, Alan Grupioni</creator><creator>Motta, Ana Carolina F.</creator><general>American Academy of Periodontology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-2603-8467</orcidid><orcidid>https://orcid.org/0000-0002-8830-4273</orcidid><orcidid>https://orcid.org/0000-0002-6522-895X</orcidid><orcidid>https://orcid.org/0000-0002-9226-1149</orcidid><orcidid>https://orcid.org/0000-0002-3887-9239</orcidid><orcidid>https://orcid.org/0000-0002-5887-8663</orcidid><orcidid>https://orcid.org/0000-0002-5607-1460</orcidid><orcidid>https://orcid.org/0000-0003-3159-5687</orcidid><orcidid>https://orcid.org/0000-0002-4005-157X</orcidid></search><sort><creationdate>202204</creationdate><title>Gingival tissue as a reservoir for human immunodeficiency virus type 1: Preliminary results of a cross‐sectional observational study</title><author>Sereme, Youssouf ; Pólvora, Tábata Larissa S. ; Rochereau, Nicolas ; Teixeira, Lucas Ribeiro ; Santana, Rodrigo Carvalho ; Paul, Stephane ; Fonseca, Benedito Antonio Lopes ; Bourlet, Thomas ; Pozzetto, Bruno ; Lourenço, Alan Grupioni ; Motta, Ana Carolina F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3698-4694c1bb7f166d129c3495895e3ddc20c6d2a41dba2cf816eb091dafbf908953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Cross-Sectional Studies</topic><topic>gingiva</topic><topic>HIV Core Protein p24</topic><topic>HIV Infections - drug therapy</topic><topic>HIV-1 - genetics</topic><topic>HIV‐1</topic><topic>Human health and pathology</topic><topic>Humans</topic><topic>Immunology</topic><topic>Infectious diseases</topic><topic>inflammation</topic><topic>Life Sciences</topic><topic>Microbiology and Parasitology</topic><topic>mouth</topic><topic>mucous membrane</topic><topic>periodontitis</topic><topic>Pharmaceutical sciences</topic><topic>Pharmacology</topic><topic>RNA</topic><topic>saliva</topic><topic>Vaccinology</topic><topic>Viral Load</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sereme, Youssouf</creatorcontrib><creatorcontrib>Pólvora, Tábata Larissa S.</creatorcontrib><creatorcontrib>Rochereau, Nicolas</creatorcontrib><creatorcontrib>Teixeira, Lucas Ribeiro</creatorcontrib><creatorcontrib>Santana, Rodrigo Carvalho</creatorcontrib><creatorcontrib>Paul, Stephane</creatorcontrib><creatorcontrib>Fonseca, Benedito Antonio Lopes</creatorcontrib><creatorcontrib>Bourlet, Thomas</creatorcontrib><creatorcontrib>Pozzetto, Bruno</creatorcontrib><creatorcontrib>Lourenço, Alan Grupioni</creatorcontrib><creatorcontrib>Motta, Ana Carolina F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of periodontology (1970)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sereme, Youssouf</au><au>Pólvora, Tábata Larissa S.</au><au>Rochereau, Nicolas</au><au>Teixeira, Lucas Ribeiro</au><au>Santana, Rodrigo Carvalho</au><au>Paul, Stephane</au><au>Fonseca, Benedito Antonio Lopes</au><au>Bourlet, Thomas</au><au>Pozzetto, Bruno</au><au>Lourenço, Alan Grupioni</au><au>Motta, Ana Carolina F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gingival tissue as a reservoir for human immunodeficiency virus type 1: Preliminary results of a cross‐sectional observational study</atitle><jtitle>Journal of periodontology (1970)</jtitle><addtitle>J Periodontol</addtitle><date>2022-04</date><risdate>2022</risdate><volume>93</volume><issue>4</issue><spage>613</spage><epage>620</epage><pages>613-620</pages><issn>0022-3492</issn><eissn>1943-3670</eissn><abstract>Background
Despite combined antiretroviral therapy (cART), total cure of immunodeficiency virus type 1 (HIV‐1) infection remains elusive. Chronic periodontitis (CP) is strongly associated with HIV‐1 infection. This condition is characterized by an intense inflammatory infiltrate mainly constituted of immune cells which in turn may be a valuable source of HIV‐1 reactivation. This study aimed to determine if gingival tissue could act as a reservoir for HIV‐1.
Methods
Twelve patients with HIV‐1 and CP and 12 controls (no HIV‐1‐infection and no CP) were evaluated in a cross‐sectional study. RNA viral load and interleukin (IL) levels were determined in blood plasma and saliva. Histological sections of gingival tissue were stained with fluorescent antibodies against p24 antigen and different cellular biomarkers.
Results
In six of the 12 patients, HIV RNA load was detected, despite cART; in three of them, expression of viral RNA was also detected in saliva. The levels of IL‐2, IL‐6, and IL‐12 were higher in blood and saliva of patients with HIVand CP than controls. HIV‐1 p24 antigen was detected by immunostaining in gingival biopsies of 10 of the 12 patients but in no controls. Immune markers for T cells and antigen‐presenting cells were also identified in most patients and some controls.
Conclusion
These preliminary data showing the detection of HIV‐1 p24 antigen in the gingival biopsies of a significant part of patients with HIV‐1 and CP under cART together with the presence of immune cells, plead for the existence of a HIV‐1 reservoir in the gingival tissue of this population.</abstract><cop>United States</cop><pub>American Academy of Periodontology</pub><pmid>34396525</pmid><doi>10.1002/JPER.21-0345</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-2603-8467</orcidid><orcidid>https://orcid.org/0000-0002-8830-4273</orcidid><orcidid>https://orcid.org/0000-0002-6522-895X</orcidid><orcidid>https://orcid.org/0000-0002-9226-1149</orcidid><orcidid>https://orcid.org/0000-0002-3887-9239</orcidid><orcidid>https://orcid.org/0000-0002-5887-8663</orcidid><orcidid>https://orcid.org/0000-0002-5607-1460</orcidid><orcidid>https://orcid.org/0000-0003-3159-5687</orcidid><orcidid>https://orcid.org/0000-0002-4005-157X</orcidid></addata></record> |
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| ispartof | Journal of periodontology (1970), 2022-04, Vol.93 (4), p.613-620 |
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| subjects | Cross-Sectional Studies gingiva HIV Core Protein p24 HIV Infections - drug therapy HIV-1 - genetics HIV‐1 Human health and pathology Humans Immunology Infectious diseases inflammation Life Sciences Microbiology and Parasitology mouth mucous membrane periodontitis Pharmaceutical sciences Pharmacology RNA saliva Vaccinology Viral Load Virology |
| title | Gingival tissue as a reservoir for human immunodeficiency virus type 1: Preliminary results of a cross‐sectional observational study |
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