Total mouth photodynamic therapy mediated by red LED and porphyrin in individuals with AIDS
Due to the immune changes resulting from HIV/AIDS infection, systemic and local infections throughout the body are common. The use of high activity antiretroviral therapy has been widely used during treatment, which, added to the use of antibiotics, antifungals, and the patients’ own immunocompromis...
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creator | da Silva, Francine Cristina Rosa, Luciano Pereira de Jesus, Iasmym Mendes de Oliveira Santos, Gabriel Pinto Inada, Natália Mayumi Blanco, Kate Cristina Araújo, Thalita Santos Dantas Bagnato, Vanderlei Salvador |
description | Due to the immune changes resulting from HIV/AIDS infection, systemic and local infections throughout the body are common. The use of high activity antiretroviral therapy has been widely used during treatment, which, added to the use of antibiotics, antifungals, and the patients’ own immunocompromised state, cause important changes in the oral microbiota. The emergence of pathological microorganisms and with high resistance to drug therapies are frequent and cause serious damage to the oral health of these patients. In this sense, antimicrobial photodynamic therapy (aPDT) appears as a promising alternative in the control of these oral infections. The aim of the study was to test the effectiveness of a therapeutic protocol for total oral aPDT mediated by a 660-nm red LED (light-emitting diode) associated with porphyrin in individuals with AIDS. Patients were selected by exclusion criteria and randomly distributed into groups to test the effectiveness of antimicrobial aPDT with 50 µg/ml porphyrin associated with the red LED. Before and after the treatments, saliva samples were collected and processed in duplicate in selective culture media. Colonies were counted and the results obtained in Log10 CFU/ml and tested statistically. It was concluded that aPDT was effective in reducing oral enterobacteria, in addition to reducing
Streptococcus
spp. and general count of microorganisms, when considering the numbers of TCD4 and TCD8 lymphocytes. |
doi_str_mv | 10.1007/s10103-021-03377-z |
format | Article |
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Streptococcus
spp. and general count of microorganisms, when considering the numbers of TCD4 and TCD8 lymphocytes.</description><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-021-03377-z</identifier><identifier>PMID: 34387786</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Acquired immune deficiency syndrome ; Acquired Immunodeficiency Syndrome - complications ; Acquired Immunodeficiency Syndrome - drug therapy ; AIDS ; Antibiotics ; Antiinfectives and antibacterials ; Antiretroviral agents ; Antiretroviral therapy ; Culture media ; Dentistry ; High resistance ; HIV ; Human immunodeficiency virus ; Humans ; Infections ; Lasers ; Light emitting diodes ; Lymphocytes ; Medicine ; Medicine & Public Health ; Microbiota ; Microorganisms ; Mouth ; Optical Devices ; Optics ; Original Article ; Patients ; Photochemotherapy - methods ; Photodynamic therapy ; Photonics ; Photosensitizing Agents - therapeutic use ; Porphyrins ; Porphyrins - therapeutic use ; Quantum Optics ; Saliva ; Selective media ; Statistical methods ; Streptococcus infections</subject><ispartof>Lasers in medical science, 2022-03, Vol.37 (2), p.1227-1234</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-f03bc6058d6decc4146ad036e60e912c85f8633d6e42c1d04d928b57467b1bd73</citedby><cites>FETCH-LOGICAL-c375t-f03bc6058d6decc4146ad036e60e912c85f8633d6e42c1d04d928b57467b1bd73</cites><orcidid>0000-0002-3926-3970</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10103-021-03377-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10103-021-03377-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34387786$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Silva, Francine Cristina</creatorcontrib><creatorcontrib>Rosa, Luciano Pereira</creatorcontrib><creatorcontrib>de Jesus, Iasmym Mendes</creatorcontrib><creatorcontrib>de Oliveira Santos, Gabriel Pinto</creatorcontrib><creatorcontrib>Inada, Natália Mayumi</creatorcontrib><creatorcontrib>Blanco, Kate Cristina</creatorcontrib><creatorcontrib>Araújo, Thalita Santos Dantas</creatorcontrib><creatorcontrib>Bagnato, Vanderlei Salvador</creatorcontrib><title>Total mouth photodynamic therapy mediated by red LED and porphyrin in individuals with AIDS</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><addtitle>Lasers Med Sci</addtitle><description>Due to the immune changes resulting from HIV/AIDS infection, systemic and local infections throughout the body are common. The use of high activity antiretroviral therapy has been widely used during treatment, which, added to the use of antibiotics, antifungals, and the patients’ own immunocompromised state, cause important changes in the oral microbiota. The emergence of pathological microorganisms and with high resistance to drug therapies are frequent and cause serious damage to the oral health of these patients. In this sense, antimicrobial photodynamic therapy (aPDT) appears as a promising alternative in the control of these oral infections. The aim of the study was to test the effectiveness of a therapeutic protocol for total oral aPDT mediated by a 660-nm red LED (light-emitting diode) associated with porphyrin in individuals with AIDS. Patients were selected by exclusion criteria and randomly distributed into groups to test the effectiveness of antimicrobial aPDT with 50 µg/ml porphyrin associated with the red LED. Before and after the treatments, saliva samples were collected and processed in duplicate in selective culture media. Colonies were counted and the results obtained in Log10 CFU/ml and tested statistically. It was concluded that aPDT was effective in reducing oral enterobacteria, in addition to reducing
Streptococcus
spp. and general count of microorganisms, when considering the numbers of TCD4 and TCD8 lymphocytes.</description><subject>Acquired immune deficiency syndrome</subject><subject>Acquired Immunodeficiency Syndrome - complications</subject><subject>Acquired Immunodeficiency Syndrome - drug therapy</subject><subject>AIDS</subject><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Antiretroviral agents</subject><subject>Antiretroviral therapy</subject><subject>Culture media</subject><subject>Dentistry</subject><subject>High resistance</subject><subject>HIV</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Infections</subject><subject>Lasers</subject><subject>Light emitting diodes</subject><subject>Lymphocytes</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Mouth</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Original Article</subject><subject>Patients</subject><subject>Photochemotherapy - methods</subject><subject>Photodynamic therapy</subject><subject>Photonics</subject><subject>Photosensitizing Agents - therapeutic use</subject><subject>Porphyrins</subject><subject>Porphyrins - therapeutic use</subject><subject>Quantum Optics</subject><subject>Saliva</subject><subject>Selective media</subject><subject>Statistical methods</subject><subject>Streptococcus infections</subject><issn>0268-8921</issn><issn>1435-604X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kM9LHDEUx0Op6Lr1H-ihBHrxMvXlxySZ47JqFRY8qFDwEDJJtjuyM5kmMy3jX9-4qy14EAIP8j7v-x4fhD4T-EYA5FkiQIAVQEkBjElZPH1AM8JZWQjgPz6iGVChClVRcoSOU3oEIFIQdoiOGGdKSiVm6OEuDGaL2zAOG9xvwhDc1Jm2sXjY-Gj6CbfeNWbwDtcTjrmsLs6x6RzuQ-w3U2w6vHuu-d240WwT_tPkqMX1-e0ndLDOH_7kpc7R_eXF3fKqWN18v14uVoVlshyKNbDaCiiVE85bywkXxgETXoCvCLWqXCvBmBOeU0sccFdRVZeSC1mT2kk2R6f73D6GX6NPg26bZP12azofxqRpKQivoAKR0a9v0Mcwxi5fp6lgihFKaZUpuqdsDClFv9Z9bFoTJ01AP6vXe_U6q9c79fopD315iR7r7OzfyKvrDLA9kHKr--nj_93vxP4FIHyOYQ</recordid><startdate>20220301</startdate><enddate>20220301</enddate><creator>da Silva, Francine Cristina</creator><creator>Rosa, Luciano Pereira</creator><creator>de Jesus, Iasmym Mendes</creator><creator>de Oliveira Santos, Gabriel Pinto</creator><creator>Inada, Natália Mayumi</creator><creator>Blanco, Kate Cristina</creator><creator>Araújo, Thalita Santos Dantas</creator><creator>Bagnato, Vanderlei Salvador</creator><general>Springer London</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7SP</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3926-3970</orcidid></search><sort><creationdate>20220301</creationdate><title>Total mouth photodynamic therapy mediated by red LED and porphyrin in individuals with AIDS</title><author>da Silva, Francine Cristina ; Rosa, Luciano Pereira ; de Jesus, Iasmym Mendes ; de Oliveira Santos, Gabriel Pinto ; Inada, Natália Mayumi ; Blanco, Kate Cristina ; Araújo, Thalita Santos Dantas ; Bagnato, Vanderlei Salvador</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-f03bc6058d6decc4146ad036e60e912c85f8633d6e42c1d04d928b57467b1bd73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Acquired immune deficiency syndrome</topic><topic>Acquired Immunodeficiency Syndrome - 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Academic</collection><jtitle>Lasers in medical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>da Silva, Francine Cristina</au><au>Rosa, Luciano Pereira</au><au>de Jesus, Iasmym Mendes</au><au>de Oliveira Santos, Gabriel Pinto</au><au>Inada, Natália Mayumi</au><au>Blanco, Kate Cristina</au><au>Araújo, Thalita Santos Dantas</au><au>Bagnato, Vanderlei Salvador</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Total mouth photodynamic therapy mediated by red LED and porphyrin in individuals with AIDS</atitle><jtitle>Lasers in medical science</jtitle><stitle>Lasers Med Sci</stitle><addtitle>Lasers Med Sci</addtitle><date>2022-03-01</date><risdate>2022</risdate><volume>37</volume><issue>2</issue><spage>1227</spage><epage>1234</epage><pages>1227-1234</pages><issn>0268-8921</issn><eissn>1435-604X</eissn><abstract>Due to the immune changes resulting from HIV/AIDS infection, systemic and local infections throughout the body are common. The use of high activity antiretroviral therapy has been widely used during treatment, which, added to the use of antibiotics, antifungals, and the patients’ own immunocompromised state, cause important changes in the oral microbiota. The emergence of pathological microorganisms and with high resistance to drug therapies are frequent and cause serious damage to the oral health of these patients. In this sense, antimicrobial photodynamic therapy (aPDT) appears as a promising alternative in the control of these oral infections. The aim of the study was to test the effectiveness of a therapeutic protocol for total oral aPDT mediated by a 660-nm red LED (light-emitting diode) associated with porphyrin in individuals with AIDS. Patients were selected by exclusion criteria and randomly distributed into groups to test the effectiveness of antimicrobial aPDT with 50 µg/ml porphyrin associated with the red LED. Before and after the treatments, saliva samples were collected and processed in duplicate in selective culture media. Colonies were counted and the results obtained in Log10 CFU/ml and tested statistically. It was concluded that aPDT was effective in reducing oral enterobacteria, in addition to reducing
Streptococcus
spp. and general count of microorganisms, when considering the numbers of TCD4 and TCD8 lymphocytes.</abstract><cop>London</cop><pub>Springer London</pub><pmid>34387786</pmid><doi>10.1007/s10103-021-03377-z</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-3926-3970</orcidid></addata></record> |
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subjects | Acquired immune deficiency syndrome Acquired Immunodeficiency Syndrome - complications Acquired Immunodeficiency Syndrome - drug therapy AIDS Antibiotics Antiinfectives and antibacterials Antiretroviral agents Antiretroviral therapy Culture media Dentistry High resistance HIV Human immunodeficiency virus Humans Infections Lasers Light emitting diodes Lymphocytes Medicine Medicine & Public Health Microbiota Microorganisms Mouth Optical Devices Optics Original Article Patients Photochemotherapy - methods Photodynamic therapy Photonics Photosensitizing Agents - therapeutic use Porphyrins Porphyrins - therapeutic use Quantum Optics Saliva Selective media Statistical methods Streptococcus infections |
title | Total mouth photodynamic therapy mediated by red LED and porphyrin in individuals with AIDS |
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