Radiation mode and tissue thickness impact on singlet oxygen dosimetry methods for antimicrobial photodynamic therapy

•The dosimetry method's singlet oxygen measurement capability for deeply infected tissue was explored.•Methylene blue AmPDT application was investigated for deeper infected tissues.•Hypoxia prevention with radiation mode was investigated.•The performances of super pulse and pulse modes were investigated. The target of the presented study is to evaluate the performances of illumination modes on Photodynamic therapy (PDT) for different tissue depths. For this purpose, radiation-based super pulse and pulse illumination modes were investigated for antimicrobial PDT (AmPDT). Singlet oxygen luminescence level was measured from two different points. The first one was to appraise the light penetration depth effect on singlet oxygen luminescence level for various radiation modes. The second one explored the singlet oxygen luminescence dosimetry (SOLD) method from deeper photosensitizer accumulated tissue levels. Two main experiments were performed in this study. The singlet oxygen concentration was calculated with singlet oxygen explicit dosimetry (SOED) and SOLD methods for various tissue depths in these experiments. According to the results of the experiments, super pulse mode (SPM) provided relatively high Staphylococcus Aureus (S. aureus) cell death by 5–12%. The penetration depth was increased between 0.2 mm and 0.7 mm during the experiments. SOLD-based singlet oxygen detection system was utilized to detect singlet oxygen production levels from various tissue thicknesses to evaluate the system's usefulness for deeper infected tissues. It was observed that SPM was more effective than pulse mode radiation after a certain tissue depth (≤ 2 mm).