Directed Evolution of an RNA Enzyme

An in vitro evolution procedure was used to obtain RNA enzymes with a particular catalytic function. A population of 10$^{13}$ variants of the Tetrahymena ribozyme, a group I ribozyme that catalyzes sequence-specific cleavage of RNA via a phosphoester transfer mechanism, was generated. This enzyme h...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 1992-07, Vol.257 (5070), p.635-641
Hauptverfasser:	Beaudry, Amber A., Joyce, Gerald F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Base Composition Base Sequence Biological and medical sciences Catalysis Catalytic RNA Chemical evolution Chemistry Deoxyribonucleic acid DNA DNA cleavage DNA, Single-Stranded - metabolism Engineering Enzymes Evolution Fundamental and applied biological sciences. Psychology Gels Genetic mutation Genotype Hot Temperature Hydrolysis Legends Life Sciences (General) Magnesium Chloride - pharmacology Molecular evolution Molecular Sequence Data Mutagenesis Mutagenesis, Site-Directed Mutation Mutation (Biology) Nucleic acids Phenotype Polymerase Chain Reaction Research Article Ribonucleic acid RNA RNA, Catalytic - genetics RNA, Catalytic - metabolism Rna, ribonucleoproteins Substrate Specificity Tetrahymena Tetrahymena thermophila - genetics
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