NEAT1 Boosts the Development of Thoracic Aortic Aneurysm Through Targeting miR-324-5p/RAN
Long non-coding RNAs (lncRNAs) have been identified in multiple cancers. Recently, NEAT1 is found to be up regulated in cervical cancer. Since the relationship between NEAT1 and thoracic aortic aneurysm (TAA) has not been clarified, our study focused on the role of NEAT1 in TAA. Bioinformatics, RNA...
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| Veröffentlicht in: | Archives of medical research 2022-01, Vol.53 (1), p.93-99 |
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| creator | Gao, Cheng Sun, Jifeng Zhang, Zhihua Xu, Zhaoxun |
| description | Long non-coding RNAs (lncRNAs) have been identified in multiple cancers. Recently, NEAT1 is found to be up regulated in cervical cancer. Since the relationship between NEAT1 and thoracic aortic aneurysm (TAA) has not been clarified, our study focused on the role of NEAT1 in TAA.
Bioinformatics, RNA pulls down and luciferase reporter assay were used to discover and determine miR-324-5p to be a target of NEAT1. RT-qPCR was used to examine NEAT1, RAN and miR-324-5p expression.
NEAT1 was up-regulated in TAA patients, as well as HAoSMC and HA-VSMC cells. Down-regulation of NEAT1 could inhibit the proliferative abilities while promoting apoptosis of TAA cells. MiR-324-5p expression was down-regulated in both TAA tissues and cells. Then, RAN was selected out as a target of miR-324-5p. More interestingly, miR-324-5p had inhibitory effects on malignant behaviors of TAA cells. RAN was negatively related with miR-324-5p while positively correlated with NEAT1 in the tissues. Finally, the data of rescue assays manifested that RAN up-regulation could countervail the influence of down-regulation of NEAT1 on TAA cells.
NEAT1 could contribute to the malignant behaviors of TAA cells by targeting miR-324-5p/RAN. NEAT1 might be an underlying target for the therapy of TAA. |
| doi_str_mv | 10.1016/j.arcmed.2021.06.009 |
| format | Article |
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Bioinformatics, RNA pulls down and luciferase reporter assay were used to discover and determine miR-324-5p to be a target of NEAT1. RT-qPCR was used to examine NEAT1, RAN and miR-324-5p expression.
NEAT1 was up-regulated in TAA patients, as well as HAoSMC and HA-VSMC cells. Down-regulation of NEAT1 could inhibit the proliferative abilities while promoting apoptosis of TAA cells. MiR-324-5p expression was down-regulated in both TAA tissues and cells. Then, RAN was selected out as a target of miR-324-5p. More interestingly, miR-324-5p had inhibitory effects on malignant behaviors of TAA cells. RAN was negatively related with miR-324-5p while positively correlated with NEAT1 in the tissues. Finally, the data of rescue assays manifested that RAN up-regulation could countervail the influence of down-regulation of NEAT1 on TAA cells.
NEAT1 could contribute to the malignant behaviors of TAA cells by targeting miR-324-5p/RAN. NEAT1 might be an underlying target for the therapy of TAA.</description><identifier>ISSN: 0188-4409</identifier><identifier>EISSN: 1873-5487</identifier><identifier>DOI: 10.1016/j.arcmed.2021.06.009</identifier><identifier>PMID: 34373133</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Aortic Aneurysm, Thoracic - genetics ; Cell Proliferation - genetics ; Down-Regulation ; Humans ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miR-324-5p ; NEAT1 ; RAN ; ran GTP-Binding Protein ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; Thoracic aortic aneurysm</subject><ispartof>Archives of medical research, 2022-01, Vol.53 (1), p.93-99</ispartof><rights>2021</rights><rights>Copyright © 2021. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-51580030a48a3309231f170818212533263031c9ef7140a1a33760bf9f422c333</citedby><cites>FETCH-LOGICAL-c362t-51580030a48a3309231f170818212533263031c9ef7140a1a33760bf9f422c333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.arcmed.2021.06.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34373133$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Cheng</creatorcontrib><creatorcontrib>Sun, Jifeng</creatorcontrib><creatorcontrib>Zhang, Zhihua</creatorcontrib><creatorcontrib>Xu, Zhaoxun</creatorcontrib><title>NEAT1 Boosts the Development of Thoracic Aortic Aneurysm Through Targeting miR-324-5p/RAN</title><title>Archives of medical research</title><addtitle>Arch Med Res</addtitle><description>Long non-coding RNAs (lncRNAs) have been identified in multiple cancers. Recently, NEAT1 is found to be up regulated in cervical cancer. Since the relationship between NEAT1 and thoracic aortic aneurysm (TAA) has not been clarified, our study focused on the role of NEAT1 in TAA.
Bioinformatics, RNA pulls down and luciferase reporter assay were used to discover and determine miR-324-5p to be a target of NEAT1. RT-qPCR was used to examine NEAT1, RAN and miR-324-5p expression.
NEAT1 was up-regulated in TAA patients, as well as HAoSMC and HA-VSMC cells. Down-regulation of NEAT1 could inhibit the proliferative abilities while promoting apoptosis of TAA cells. MiR-324-5p expression was down-regulated in both TAA tissues and cells. Then, RAN was selected out as a target of miR-324-5p. More interestingly, miR-324-5p had inhibitory effects on malignant behaviors of TAA cells. RAN was negatively related with miR-324-5p while positively correlated with NEAT1 in the tissues. Finally, the data of rescue assays manifested that RAN up-regulation could countervail the influence of down-regulation of NEAT1 on TAA cells.
NEAT1 could contribute to the malignant behaviors of TAA cells by targeting miR-324-5p/RAN. NEAT1 might be an underlying target for the therapy of TAA.</description><subject>Aortic Aneurysm, Thoracic - genetics</subject><subject>Cell Proliferation - genetics</subject><subject>Down-Regulation</subject><subject>Humans</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miR-324-5p</subject><subject>NEAT1</subject><subject>RAN</subject><subject>ran GTP-Binding Protein</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>Thoracic aortic aneurysm</subject><issn>0188-4409</issn><issn>1873-5487</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFu1DAQhi0EotvCGyCUI5ekMx7HcS5IS1sKUlWkajlwslzvZNerTbzYSaW-PVlte-X0H-ab-TWfEJ8QKgTUl7vKJd_zupIgsQJdAbRvxAJNQ2WtTPNWLACNKZWC9kyc57wDAKN0816ckaKGkGgh_tzfLFdYfIsxj7kYt1xc8xPv46HnYSxiV6y2MTkffLGMaTzGwFN6zv08SHHabIuVSxsew7Ap-vBQklRlfbh8WN5_EO86t8_88SUvxO_vN6urH-Xdr9ufV8u70pOWY1ljbQAInDKOCFpJ2GEDBo1EWRNJTUDoW-4aVOBwhhoNj13bKSk9EV2IL6e7hxT_TpxH24fseb93A8cpW1lrgLoBpWdUnVCfYs6JO3tIoXfp2SLYo1S7syep9ijVgraz1Hnt80vD9HicvS69WpyBryeA5z-fAiebfeDB8zok9qNdx_D_hn9TJ4WA</recordid><startdate>202201</startdate><enddate>202201</enddate><creator>Gao, Cheng</creator><creator>Sun, Jifeng</creator><creator>Zhang, Zhihua</creator><creator>Xu, Zhaoxun</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202201</creationdate><title>NEAT1 Boosts the Development of Thoracic Aortic Aneurysm Through Targeting miR-324-5p/RAN</title><author>Gao, Cheng ; Sun, Jifeng ; Zhang, Zhihua ; Xu, Zhaoxun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-51580030a48a3309231f170818212533263031c9ef7140a1a33760bf9f422c333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aortic Aneurysm, Thoracic - genetics</topic><topic>Cell Proliferation - genetics</topic><topic>Down-Regulation</topic><topic>Humans</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miR-324-5p</topic><topic>NEAT1</topic><topic>RAN</topic><topic>ran GTP-Binding Protein</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>Thoracic aortic aneurysm</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Cheng</creatorcontrib><creatorcontrib>Sun, Jifeng</creatorcontrib><creatorcontrib>Zhang, Zhihua</creatorcontrib><creatorcontrib>Xu, Zhaoxun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of medical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Cheng</au><au>Sun, Jifeng</au><au>Zhang, Zhihua</au><au>Xu, Zhaoxun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>NEAT1 Boosts the Development of Thoracic Aortic Aneurysm Through Targeting miR-324-5p/RAN</atitle><jtitle>Archives of medical research</jtitle><addtitle>Arch Med Res</addtitle><date>2022-01</date><risdate>2022</risdate><volume>53</volume><issue>1</issue><spage>93</spage><epage>99</epage><pages>93-99</pages><issn>0188-4409</issn><eissn>1873-5487</eissn><abstract>Long non-coding RNAs (lncRNAs) have been identified in multiple cancers. Recently, NEAT1 is found to be up regulated in cervical cancer. Since the relationship between NEAT1 and thoracic aortic aneurysm (TAA) has not been clarified, our study focused on the role of NEAT1 in TAA.
Bioinformatics, RNA pulls down and luciferase reporter assay were used to discover and determine miR-324-5p to be a target of NEAT1. RT-qPCR was used to examine NEAT1, RAN and miR-324-5p expression.
NEAT1 was up-regulated in TAA patients, as well as HAoSMC and HA-VSMC cells. Down-regulation of NEAT1 could inhibit the proliferative abilities while promoting apoptosis of TAA cells. MiR-324-5p expression was down-regulated in both TAA tissues and cells. Then, RAN was selected out as a target of miR-324-5p. More interestingly, miR-324-5p had inhibitory effects on malignant behaviors of TAA cells. RAN was negatively related with miR-324-5p while positively correlated with NEAT1 in the tissues. Finally, the data of rescue assays manifested that RAN up-regulation could countervail the influence of down-regulation of NEAT1 on TAA cells.
NEAT1 could contribute to the malignant behaviors of TAA cells by targeting miR-324-5p/RAN. NEAT1 might be an underlying target for the therapy of TAA.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>34373133</pmid><doi>10.1016/j.arcmed.2021.06.009</doi><tpages>7</tpages></addata></record> |
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| subjects | Aortic Aneurysm, Thoracic - genetics Cell Proliferation - genetics Down-Regulation Humans MicroRNAs - genetics MicroRNAs - metabolism miR-324-5p NEAT1 RAN ran GTP-Binding Protein RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism Thoracic aortic aneurysm |
| title | NEAT1 Boosts the Development of Thoracic Aortic Aneurysm Through Targeting miR-324-5p/RAN |
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