Changing the shape of wool yarns via laccase-mediated grafting of tyrosine

[Display omitted] •l-cysteine was used as pre-treatment to break disulphide bonds.•The shape of wool yarns was changed by tyrosine grafting.•Laccase was used as catalyst for the tyrosine grafting. The shape of wool yarns was changed by laccase-assisted grafting of tyrosine. Prior to tyrosine graftin...

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Veröffentlicht in:Journal of biotechnology 2021-09, Vol.339, p.73-80
Hauptverfasser: Li, Yu, Noro, Jennifer, Martins, Madalena, Jing, Su, Silva, Carla, Cavaco-Paulo, Artur
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container_end_page 80
container_issue
container_start_page 73
container_title Journal of biotechnology
container_volume 339
creator Li, Yu
Noro, Jennifer
Martins, Madalena
Jing, Su
Silva, Carla
Cavaco-Paulo, Artur
description [Display omitted] •l-cysteine was used as pre-treatment to break disulphide bonds.•The shape of wool yarns was changed by tyrosine grafting.•Laccase was used as catalyst for the tyrosine grafting. The shape of wool yarns was changed by laccase-assisted grafting of tyrosine. Prior to tyrosine grafting a cysteine pre-treatment was optimized aiming to increase the amount of thiol reaction groups available. The best operational conditions for laccase-assisted tyrosine grafting were: i) pre-treatment with cysteine (2.2 mM) in a solution of 20 % ethanol, 15 % propylene glycol and 0.5 % benzyl alcohol, pH = 10, 40 °C; ii) tyrosine grafting with 3.0 mM tyrosine, 18 U/mL laccase, pH = 5, 40 °C. The shape modification was evaluated by number of curly twists determination on the grafted yarn samples. The thermal and mechanical properties of the grafted wool yarns was evaluated by TGA, DSC and breaking strength determination. The amount of free thiols and weight gain were assessed aiming to infer the role of the cysteine pre-treatment on the final tyrosine grafting and shape modification. The laccase-assisted grafting of tyrosine onto wool yarns have influenced the thermal and mechanical properties of the yarns however without compromising their structural integrity for the final application purposes. The developed methodology to impart new shape to wool yarns is presented herein as an environmentally friendly alternative to chemical methods. The new findings revealed great potentialities for application in similar fibers like hair.
doi_str_mv 10.1016/j.jbiotec.2021.08.001
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The shape of wool yarns was changed by laccase-assisted grafting of tyrosine. Prior to tyrosine grafting a cysteine pre-treatment was optimized aiming to increase the amount of thiol reaction groups available. The best operational conditions for laccase-assisted tyrosine grafting were: i) pre-treatment with cysteine (2.2 mM) in a solution of 20 % ethanol, 15 % propylene glycol and 0.5 % benzyl alcohol, pH = 10, 40 °C; ii) tyrosine grafting with 3.0 mM tyrosine, 18 U/mL laccase, pH = 5, 40 °C. The shape modification was evaluated by number of curly twists determination on the grafted yarn samples. The thermal and mechanical properties of the grafted wool yarns was evaluated by TGA, DSC and breaking strength determination. The amount of free thiols and weight gain were assessed aiming to infer the role of the cysteine pre-treatment on the final tyrosine grafting and shape modification. The laccase-assisted grafting of tyrosine onto wool yarns have influenced the thermal and mechanical properties of the yarns however without compromising their structural integrity for the final application purposes. The developed methodology to impart new shape to wool yarns is presented herein as an environmentally friendly alternative to chemical methods. 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The laccase-assisted grafting of tyrosine onto wool yarns have influenced the thermal and mechanical properties of the yarns however without compromising their structural integrity for the final application purposes. The developed methodology to impart new shape to wool yarns is presented herein as an environmentally friendly alternative to chemical methods. The new findings revealed great potentialities for application in similar fibers like hair.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.jbiotec.2021.08.001</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-5480-937X</orcidid><oa>free_for_read</oa></addata></record>
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subjects Laccase
Shape modeling
Tyrosine grafting
Wool yarns
title Changing the shape of wool yarns via laccase-mediated grafting of tyrosine
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