ALP-activated probe for diagnosis of liver injury by multispectral optoacoustic tomography

In this chapter, we highlight the advantages of multispectral optoacoustic tomography (MSOT) technique and the activatable photoacoustic probes in the biomedical field, and give a brief introduction to enzyme-activated probes for disease diagnosis and therapeutic outcome evaluation. We also present a detailed description of the procedures for the synthesis of an activatable small molecule probe C X-OR and confirmation of its specific response to alkaline phosphatase in solution and cells. With MSOT, the liposomal C X-OR can be utilized for detection of hepatic ALP as well as for in vivo diagnosis of drug-induced liver injury in a three-dimensional manner.