In vitro and in vivo immunoregulatory activity of sulfated fucan from the sea cucumber A. leucoprocta

The in vitro and in vivo immunoregulatory activity of a water-soluble sulfated fucan AL1-1 from the sea cucumber A. leucoprocta was elucidated. In vitro experiments showed that AL1-1 up-regulated immunostimulatory activities in RAW264.7 cells and that it could successfully promote ROS production and...

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Veröffentlicht in:International journal of biological macromolecules 2021-09, Vol.187, p.931-938
Hauptverfasser: Feng, Guo, Laijin, Su, Chen, Shen, Teng, Wang, Dejian, Zhou, Yin, Chen, Shoudong, Guo
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container_title International journal of biological macromolecules
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creator Feng, Guo
Laijin, Su
Chen, Shen
Teng, Wang
Dejian, Zhou
Yin, Chen
Shoudong, Guo
description The in vitro and in vivo immunoregulatory activity of a water-soluble sulfated fucan AL1-1 from the sea cucumber A. leucoprocta was elucidated. In vitro experiments showed that AL1-1 up-regulated immunostimulatory activities in RAW264.7 cells and that it could successfully promote ROS production and phagocytic activity, increase secretion levels of iNOS, and induce the production of considerable amounts of cytokines (TNF-α, IL-6, IL-1β and IL-12). We found that toll-like receptor 4 (TLR4) was mainly involved in AL1-1 mediated macrophage activation. AL1-1's in vivo immunomodulatory activity on cyclophosphamide (CY)-treated mice was investigated and it was shown that it could strongly enhance Sig A levels, promote the total antioxidant capacity (T-AOC), and reduce malondialdehyde (MDA) level in the intestine. It could also increase activities of superoxidase dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX). These results demonstrate that AL1-1 has a significant effect on enhancing in vivo and in vitro immune response.
doi_str_mv 10.1016/j.ijbiomac.2021.08.008
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subjects Animals
Antioxidants - metabolism
Cytokines - metabolism
Female
Free Radical Scavengers - isolation & purification
Free Radical Scavengers - pharmacology
Immunity, Mucosal - drug effects
Immunoglobulin A, Secretory - metabolism
Immunomodulating Agents - isolation & purification
Immunomodulating Agents - pharmacology
Immunomodulation
Intestinal Mucosa - drug effects
Intestinal Mucosa - immunology
Intestinal Mucosa - metabolism
Macrophage Activation - drug effects
Macrophages - drug effects
Macrophages - immunology
Macrophages - metabolism
Mice
Mice, Inbred ICR
Nitric Oxide Synthase Type II - metabolism
Phagocytosis - drug effects
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
RAW 264.7 Cells
Reactive Oxygen Species - metabolism
Sea cucumber
Sea Cucumbers - chemistry
Sulfated fucan
Toll-Like Receptor 4 - metabolism
title In vitro and in vivo immunoregulatory activity of sulfated fucan from the sea cucumber A. leucoprocta
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