In vitro and in vivo immunoregulatory activity of sulfated fucan from the sea cucumber A. leucoprocta
The in vitro and in vivo immunoregulatory activity of a water-soluble sulfated fucan AL1-1 from the sea cucumber A. leucoprocta was elucidated. In vitro experiments showed that AL1-1 up-regulated immunostimulatory activities in RAW264.7 cells and that it could successfully promote ROS production and...
Gespeichert in:
Veröffentlicht in: | International journal of biological macromolecules 2021-09, Vol.187, p.931-938 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 938 |
---|---|
container_issue | |
container_start_page | 931 |
container_title | International journal of biological macromolecules |
container_volume | 187 |
creator | Feng, Guo Laijin, Su Chen, Shen Teng, Wang Dejian, Zhou Yin, Chen Shoudong, Guo |
description | The in vitro and in vivo immunoregulatory activity of a water-soluble sulfated fucan AL1-1 from the sea cucumber A. leucoprocta was elucidated. In vitro experiments showed that AL1-1 up-regulated immunostimulatory activities in RAW264.7 cells and that it could successfully promote ROS production and phagocytic activity, increase secretion levels of iNOS, and induce the production of considerable amounts of cytokines (TNF-α, IL-6, IL-1β and IL-12). We found that toll-like receptor 4 (TLR4) was mainly involved in AL1-1 mediated macrophage activation. AL1-1's in vivo immunomodulatory activity on cyclophosphamide (CY)-treated mice was investigated and it was shown that it could strongly enhance Sig A levels, promote the total antioxidant capacity (T-AOC), and reduce malondialdehyde (MDA) level in the intestine. It could also increase activities of superoxidase dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX). These results demonstrate that AL1-1 has a significant effect on enhancing in vivo and in vitro immune response. |
doi_str_mv | 10.1016/j.ijbiomac.2021.08.008 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2559427617</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0141813021016676</els_id><sourcerecordid>2559427617</sourcerecordid><originalsourceid>FETCH-LOGICAL-c368t-6dd7f86cfbd90fec6b966d7e466bbaf3bf58306e1471c23e2b31c46bb2b23e233</originalsourceid><addsrcrecordid>eNqFkE1v3CAQhlHVqNkm_QsRx17sgvFi9tYoaj6kSL20ZwTD0LKyTcrHSvvvy2qTXntCwzwzr-Yh5IaznjMuv-z7sLchLgb6gQ28Z6pnTL0jG66mXccYE-_JhvGRd4oLdkk-5rxvv3LL1QdyKUYhhRrGDcGnlR5CSZGa1dFwKg6RhmWpa0z4q86mxHSkBkpo2JFGT3OdvSnoqK9gVupTXGj5jTSjoVChLhYTve3pjBXiS4pQzDW58GbO-On1vSI_77_9uHvsnr8_PN3dPncgpCqddG7ySoK3bsc8grQ7Kd2Eo5TWGi-s3yrBJPJx4jAIHKzgMLbeYE-VEFfk83lvi_1TMRe9hAw4z2bFWLMettvdOEySTw2VZxRSzDmh1y8pLCYdNWf6pFjv9ZtifVKsmdJNcRu8ec2odkH3b-zNaQO-ngFslx4CJp0h4AroQkIo2sXwv4y_8eqSAw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2559427617</pqid></control><display><type>article</type><title>In vitro and in vivo immunoregulatory activity of sulfated fucan from the sea cucumber A. leucoprocta</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Feng, Guo ; Laijin, Su ; Chen, Shen ; Teng, Wang ; Dejian, Zhou ; Yin, Chen ; Shoudong, Guo</creator><creatorcontrib>Feng, Guo ; Laijin, Su ; Chen, Shen ; Teng, Wang ; Dejian, Zhou ; Yin, Chen ; Shoudong, Guo</creatorcontrib><description>The in vitro and in vivo immunoregulatory activity of a water-soluble sulfated fucan AL1-1 from the sea cucumber A. leucoprocta was elucidated. In vitro experiments showed that AL1-1 up-regulated immunostimulatory activities in RAW264.7 cells and that it could successfully promote ROS production and phagocytic activity, increase secretion levels of iNOS, and induce the production of considerable amounts of cytokines (TNF-α, IL-6, IL-1β and IL-12). We found that toll-like receptor 4 (TLR4) was mainly involved in AL1-1 mediated macrophage activation. AL1-1's in vivo immunomodulatory activity on cyclophosphamide (CY)-treated mice was investigated and it was shown that it could strongly enhance Sig A levels, promote the total antioxidant capacity (T-AOC), and reduce malondialdehyde (MDA) level in the intestine. It could also increase activities of superoxidase dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX). These results demonstrate that AL1-1 has a significant effect on enhancing in vivo and in vitro immune response.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2021.08.008</identifier><identifier>PMID: 34363824</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antioxidants - metabolism ; Cytokines - metabolism ; Female ; Free Radical Scavengers - isolation & purification ; Free Radical Scavengers - pharmacology ; Immunity, Mucosal - drug effects ; Immunoglobulin A, Secretory - metabolism ; Immunomodulating Agents - isolation & purification ; Immunomodulating Agents - pharmacology ; Immunomodulation ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - immunology ; Intestinal Mucosa - metabolism ; Macrophage Activation - drug effects ; Macrophages - drug effects ; Macrophages - immunology ; Macrophages - metabolism ; Mice ; Mice, Inbred ICR ; Nitric Oxide Synthase Type II - metabolism ; Phagocytosis - drug effects ; Polysaccharides - isolation & purification ; Polysaccharides - pharmacology ; RAW 264.7 Cells ; Reactive Oxygen Species - metabolism ; Sea cucumber ; Sea Cucumbers - chemistry ; Sulfated fucan ; Toll-Like Receptor 4 - metabolism</subject><ispartof>International journal of biological macromolecules, 2021-09, Vol.187, p.931-938</ispartof><rights>2021</rights><rights>Copyright © 2021. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-6dd7f86cfbd90fec6b966d7e466bbaf3bf58306e1471c23e2b31c46bb2b23e233</citedby><cites>FETCH-LOGICAL-c368t-6dd7f86cfbd90fec6b966d7e466bbaf3bf58306e1471c23e2b31c46bb2b23e233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2021.08.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34363824$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feng, Guo</creatorcontrib><creatorcontrib>Laijin, Su</creatorcontrib><creatorcontrib>Chen, Shen</creatorcontrib><creatorcontrib>Teng, Wang</creatorcontrib><creatorcontrib>Dejian, Zhou</creatorcontrib><creatorcontrib>Yin, Chen</creatorcontrib><creatorcontrib>Shoudong, Guo</creatorcontrib><title>In vitro and in vivo immunoregulatory activity of sulfated fucan from the sea cucumber A. leucoprocta</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>The in vitro and in vivo immunoregulatory activity of a water-soluble sulfated fucan AL1-1 from the sea cucumber A. leucoprocta was elucidated. In vitro experiments showed that AL1-1 up-regulated immunostimulatory activities in RAW264.7 cells and that it could successfully promote ROS production and phagocytic activity, increase secretion levels of iNOS, and induce the production of considerable amounts of cytokines (TNF-α, IL-6, IL-1β and IL-12). We found that toll-like receptor 4 (TLR4) was mainly involved in AL1-1 mediated macrophage activation. AL1-1's in vivo immunomodulatory activity on cyclophosphamide (CY)-treated mice was investigated and it was shown that it could strongly enhance Sig A levels, promote the total antioxidant capacity (T-AOC), and reduce malondialdehyde (MDA) level in the intestine. It could also increase activities of superoxidase dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX). These results demonstrate that AL1-1 has a significant effect on enhancing in vivo and in vitro immune response.</description><subject>Animals</subject><subject>Antioxidants - metabolism</subject><subject>Cytokines - metabolism</subject><subject>Female</subject><subject>Free Radical Scavengers - isolation & purification</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>Immunity, Mucosal - drug effects</subject><subject>Immunoglobulin A, Secretory - metabolism</subject><subject>Immunomodulating Agents - isolation & purification</subject><subject>Immunomodulating Agents - pharmacology</subject><subject>Immunomodulation</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - immunology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Macrophage Activation - drug effects</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - immunology</subject><subject>Macrophages - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Nitric Oxide Synthase Type II - metabolism</subject><subject>Phagocytosis - drug effects</subject><subject>Polysaccharides - isolation & purification</subject><subject>Polysaccharides - pharmacology</subject><subject>RAW 264.7 Cells</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Sea cucumber</subject><subject>Sea Cucumbers - chemistry</subject><subject>Sulfated fucan</subject><subject>Toll-Like Receptor 4 - metabolism</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v3CAQhlHVqNkm_QsRx17sgvFi9tYoaj6kSL20ZwTD0LKyTcrHSvvvy2qTXntCwzwzr-Yh5IaznjMuv-z7sLchLgb6gQ28Z6pnTL0jG66mXccYE-_JhvGRd4oLdkk-5rxvv3LL1QdyKUYhhRrGDcGnlR5CSZGa1dFwKg6RhmWpa0z4q86mxHSkBkpo2JFGT3OdvSnoqK9gVupTXGj5jTSjoVChLhYTve3pjBXiS4pQzDW58GbO-On1vSI_77_9uHvsnr8_PN3dPncgpCqddG7ySoK3bsc8grQ7Kd2Eo5TWGi-s3yrBJPJx4jAIHKzgMLbeYE-VEFfk83lvi_1TMRe9hAw4z2bFWLMettvdOEySTw2VZxRSzDmh1y8pLCYdNWf6pFjv9ZtifVKsmdJNcRu8ec2odkH3b-zNaQO-ngFslx4CJp0h4AroQkIo2sXwv4y_8eqSAw</recordid><startdate>20210930</startdate><enddate>20210930</enddate><creator>Feng, Guo</creator><creator>Laijin, Su</creator><creator>Chen, Shen</creator><creator>Teng, Wang</creator><creator>Dejian, Zhou</creator><creator>Yin, Chen</creator><creator>Shoudong, Guo</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20210930</creationdate><title>In vitro and in vivo immunoregulatory activity of sulfated fucan from the sea cucumber A. leucoprocta</title><author>Feng, Guo ; Laijin, Su ; Chen, Shen ; Teng, Wang ; Dejian, Zhou ; Yin, Chen ; Shoudong, Guo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-6dd7f86cfbd90fec6b966d7e466bbaf3bf58306e1471c23e2b31c46bb2b23e233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Antioxidants - metabolism</topic><topic>Cytokines - metabolism</topic><topic>Female</topic><topic>Free Radical Scavengers - isolation & purification</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>Immunity, Mucosal - drug effects</topic><topic>Immunoglobulin A, Secretory - metabolism</topic><topic>Immunomodulating Agents - isolation & purification</topic><topic>Immunomodulating Agents - pharmacology</topic><topic>Immunomodulation</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - immunology</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Macrophage Activation - drug effects</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - immunology</topic><topic>Macrophages - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Nitric Oxide Synthase Type II - metabolism</topic><topic>Phagocytosis - drug effects</topic><topic>Polysaccharides - isolation & purification</topic><topic>Polysaccharides - pharmacology</topic><topic>RAW 264.7 Cells</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Sea cucumber</topic><topic>Sea Cucumbers - chemistry</topic><topic>Sulfated fucan</topic><topic>Toll-Like Receptor 4 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feng, Guo</creatorcontrib><creatorcontrib>Laijin, Su</creatorcontrib><creatorcontrib>Chen, Shen</creatorcontrib><creatorcontrib>Teng, Wang</creatorcontrib><creatorcontrib>Dejian, Zhou</creatorcontrib><creatorcontrib>Yin, Chen</creatorcontrib><creatorcontrib>Shoudong, Guo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feng, Guo</au><au>Laijin, Su</au><au>Chen, Shen</au><au>Teng, Wang</au><au>Dejian, Zhou</au><au>Yin, Chen</au><au>Shoudong, Guo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro and in vivo immunoregulatory activity of sulfated fucan from the sea cucumber A. leucoprocta</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2021-09-30</date><risdate>2021</risdate><volume>187</volume><spage>931</spage><epage>938</epage><pages>931-938</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>The in vitro and in vivo immunoregulatory activity of a water-soluble sulfated fucan AL1-1 from the sea cucumber A. leucoprocta was elucidated. In vitro experiments showed that AL1-1 up-regulated immunostimulatory activities in RAW264.7 cells and that it could successfully promote ROS production and phagocytic activity, increase secretion levels of iNOS, and induce the production of considerable amounts of cytokines (TNF-α, IL-6, IL-1β and IL-12). We found that toll-like receptor 4 (TLR4) was mainly involved in AL1-1 mediated macrophage activation. AL1-1's in vivo immunomodulatory activity on cyclophosphamide (CY)-treated mice was investigated and it was shown that it could strongly enhance Sig A levels, promote the total antioxidant capacity (T-AOC), and reduce malondialdehyde (MDA) level in the intestine. It could also increase activities of superoxidase dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX). These results demonstrate that AL1-1 has a significant effect on enhancing in vivo and in vitro immune response.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>34363824</pmid><doi>10.1016/j.ijbiomac.2021.08.008</doi><tpages>8</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0141-8130 |
ispartof | International journal of biological macromolecules, 2021-09, Vol.187, p.931-938 |
issn | 0141-8130 1879-0003 |
language | eng |
recordid | cdi_proquest_miscellaneous_2559427617 |
source | MEDLINE; Elsevier ScienceDirect Journals Complete |
subjects | Animals Antioxidants - metabolism Cytokines - metabolism Female Free Radical Scavengers - isolation & purification Free Radical Scavengers - pharmacology Immunity, Mucosal - drug effects Immunoglobulin A, Secretory - metabolism Immunomodulating Agents - isolation & purification Immunomodulating Agents - pharmacology Immunomodulation Intestinal Mucosa - drug effects Intestinal Mucosa - immunology Intestinal Mucosa - metabolism Macrophage Activation - drug effects Macrophages - drug effects Macrophages - immunology Macrophages - metabolism Mice Mice, Inbred ICR Nitric Oxide Synthase Type II - metabolism Phagocytosis - drug effects Polysaccharides - isolation & purification Polysaccharides - pharmacology RAW 264.7 Cells Reactive Oxygen Species - metabolism Sea cucumber Sea Cucumbers - chemistry Sulfated fucan Toll-Like Receptor 4 - metabolism |
title | In vitro and in vivo immunoregulatory activity of sulfated fucan from the sea cucumber A. leucoprocta |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T06%3A44%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20vitro%20and%20in%20vivo%20immunoregulatory%20activity%20of%20sulfated%20fucan%20from%20the%20sea%20cucumber%20A.%20leucoprocta&rft.jtitle=International%20journal%20of%20biological%20macromolecules&rft.au=Feng,%20Guo&rft.date=2021-09-30&rft.volume=187&rft.spage=931&rft.epage=938&rft.pages=931-938&rft.issn=0141-8130&rft.eissn=1879-0003&rft_id=info:doi/10.1016/j.ijbiomac.2021.08.008&rft_dat=%3Cproquest_cross%3E2559427617%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2559427617&rft_id=info:pmid/34363824&rft_els_id=S0141813021016676&rfr_iscdi=true |