The light chain of antibodies specific to the V2 region of HIV-1 can determine their function
We studied the contribution of the light chain to functions of human monoclonal antibodies (mAbs) by measuring the relationships between the rate of mutations and cross-reactivity, binding affinity and neutralization activity. We analyzed 12 mAbs of two clonal families specific to the V2 region of H...
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| Veröffentlicht in: | Human immunology 2021-12, Vol.82 (12), p.923-929 |
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| container_title | Human immunology |
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| creator | Li, Liuzhe Wang, Xiao-Hong Nanfack, Aubin Kong, Xiang-Peng Gorny, Miroslaw K. |
| description | We studied the contribution of the light chain to functions of human monoclonal antibodies (mAbs) by measuring the relationships between the rate of mutations and cross-reactivity, binding affinity and neutralization activity. We analyzed 12 mAbs of two clonal families specific to the V2 region of HIV-1 derived from two chronically HIV-1 infected individuals. The clonal mAbs exhibited a range of reactivities, and the clones with superior properties were associated with the rate of mutations and the presence of particular mutated residues in the light chains, but not in the heavy chains. Our observations suggest that for some antibodies, the light chains play a vital role in antibody evolution toward more efficient ones and also suggest the importance of optimal residues rather than the rate of mutations in the variable fragment of the antibody. |
| doi_str_mv | 10.1016/j.humimm.2021.05.004 |
| format | Article |
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We analyzed 12 mAbs of two clonal families specific to the V2 region of HIV-1 derived from two chronically HIV-1 infected individuals. The clonal mAbs exhibited a range of reactivities, and the clones with superior properties were associated with the rate of mutations and the presence of particular mutated residues in the light chains, but not in the heavy chains. Our observations suggest that for some antibodies, the light chains play a vital role in antibody evolution toward more efficient ones and also suggest the importance of optimal residues rather than the rate of mutations in the variable fragment of the antibody.</description><identifier>ISSN: 0198-8859</identifier><identifier>EISSN: 1879-1166</identifier><identifier>DOI: 10.1016/j.humimm.2021.05.004</identifier><identifier>PMID: 34340867</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Antibodies, Monoclonal - genetics ; Antibodies, Monoclonal - immunology ; Clonal antibodies ; Female ; HIV antibodies ; HIV Antibodies - genetics ; HIV Antibodies - immunology ; HIV Infections - genetics ; HIV Infections - immunology ; HIV-1 - genetics ; HIV-1 - immunology ; Humans ; Immunoglobulin Light Chains - genetics ; Immunoglobulin Light Chains - immunology ; Immunoglobulin Variable Region - genetics ; Immunoglobulin Variable Region - immunology ; Light chain of antibody ; Male ; Somatic hypermutations</subject><ispartof>Human immunology, 2021-12, Vol.82 (12), p.923-929</ispartof><rights>2021</rights><rights>Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-c58482bfdf83679f99b0fbbd7a36a41e9ebee801f9b67376cdd9ec765ce3360c3</citedby><cites>FETCH-LOGICAL-c362t-c58482bfdf83679f99b0fbbd7a36a41e9ebee801f9b67376cdd9ec765ce3360c3</cites><orcidid>0000-0002-7988-2397</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S019888592100121X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34340867$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Liuzhe</creatorcontrib><creatorcontrib>Wang, Xiao-Hong</creatorcontrib><creatorcontrib>Nanfack, Aubin</creatorcontrib><creatorcontrib>Kong, Xiang-Peng</creatorcontrib><creatorcontrib>Gorny, Miroslaw K.</creatorcontrib><title>The light chain of antibodies specific to the V2 region of HIV-1 can determine their function</title><title>Human immunology</title><addtitle>Hum Immunol</addtitle><description>We studied the contribution of the light chain to functions of human monoclonal antibodies (mAbs) by measuring the relationships between the rate of mutations and cross-reactivity, binding affinity and neutralization activity. We analyzed 12 mAbs of two clonal families specific to the V2 region of HIV-1 derived from two chronically HIV-1 infected individuals. The clonal mAbs exhibited a range of reactivities, and the clones with superior properties were associated with the rate of mutations and the presence of particular mutated residues in the light chains, but not in the heavy chains. Our observations suggest that for some antibodies, the light chains play a vital role in antibody evolution toward more efficient ones and also suggest the importance of optimal residues rather than the rate of mutations in the variable fragment of the antibody.</description><subject>Adult</subject><subject>Antibodies, Monoclonal - genetics</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Clonal antibodies</subject><subject>Female</subject><subject>HIV antibodies</subject><subject>HIV Antibodies - genetics</subject><subject>HIV Antibodies - immunology</subject><subject>HIV Infections - genetics</subject><subject>HIV Infections - immunology</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - immunology</subject><subject>Humans</subject><subject>Immunoglobulin Light Chains - genetics</subject><subject>Immunoglobulin Light Chains - immunology</subject><subject>Immunoglobulin Variable Region - genetics</subject><subject>Immunoglobulin Variable Region - immunology</subject><subject>Light chain of antibody</subject><subject>Male</subject><subject>Somatic hypermutations</subject><issn>0198-8859</issn><issn>1879-1166</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90E1P3DAQgGGrKipb2n-AkI-9JB3H8dcFCSEoSEhcgBuyEnvMerVJFtup1H9Ptgs99uTLMzPyS8gpg5oBkz839Xoe4jDUDTSsBlEDtJ_IimllKsak_ExWwIyutBbmmHzNeQMAClT7hRzzlregpVqR54c10m18WRfq1l0c6RRoN5bYTz5ipnmHLoboaJloWeRTQxO-xOmvu7l9qhh13Ug9FkxDHHGPYqJhHl1Z1DdyFLptxu_v7wl5vL56uLyp7u5_3V5e3FWOy6ZUTuhWN33wQXOpTDCmh9D3XnVcdi1Dgz2iBhZMLxVX0nlv0CkpHHIuwfET8uOwd5em1xlzsUPMDrfbbsRpzrYRQgkBWuiFtgfq0pRzwmB3KQ5d-mMZ2H1Yu7GHsHYf1oKwS9hl7Oz9wtwP6P8NfZRcwPkB4PLP3xGTzS7i6NDHhK5YP8X_X3gDFxKLeQ</recordid><startdate>202112</startdate><enddate>202112</enddate><creator>Li, Liuzhe</creator><creator>Wang, Xiao-Hong</creator><creator>Nanfack, Aubin</creator><creator>Kong, Xiang-Peng</creator><creator>Gorny, Miroslaw K.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7988-2397</orcidid></search><sort><creationdate>202112</creationdate><title>The light chain of antibodies specific to the V2 region of HIV-1 can determine their function</title><author>Li, Liuzhe ; Wang, Xiao-Hong ; Nanfack, Aubin ; Kong, Xiang-Peng ; Gorny, Miroslaw K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-c58482bfdf83679f99b0fbbd7a36a41e9ebee801f9b67376cdd9ec765ce3360c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adult</topic><topic>Antibodies, Monoclonal - genetics</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Clonal antibodies</topic><topic>Female</topic><topic>HIV antibodies</topic><topic>HIV Antibodies - genetics</topic><topic>HIV Antibodies - immunology</topic><topic>HIV Infections - genetics</topic><topic>HIV Infections - immunology</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - immunology</topic><topic>Humans</topic><topic>Immunoglobulin Light Chains - genetics</topic><topic>Immunoglobulin Light Chains - immunology</topic><topic>Immunoglobulin Variable Region - genetics</topic><topic>Immunoglobulin Variable Region - immunology</topic><topic>Light chain of antibody</topic><topic>Male</topic><topic>Somatic hypermutations</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Liuzhe</creatorcontrib><creatorcontrib>Wang, Xiao-Hong</creatorcontrib><creatorcontrib>Nanfack, Aubin</creatorcontrib><creatorcontrib>Kong, Xiang-Peng</creatorcontrib><creatorcontrib>Gorny, Miroslaw K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Liuzhe</au><au>Wang, Xiao-Hong</au><au>Nanfack, Aubin</au><au>Kong, Xiang-Peng</au><au>Gorny, Miroslaw K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The light chain of antibodies specific to the V2 region of HIV-1 can determine their function</atitle><jtitle>Human immunology</jtitle><addtitle>Hum Immunol</addtitle><date>2021-12</date><risdate>2021</risdate><volume>82</volume><issue>12</issue><spage>923</spage><epage>929</epage><pages>923-929</pages><issn>0198-8859</issn><eissn>1879-1166</eissn><abstract>We studied the contribution of the light chain to functions of human monoclonal antibodies (mAbs) by measuring the relationships between the rate of mutations and cross-reactivity, binding affinity and neutralization activity. We analyzed 12 mAbs of two clonal families specific to the V2 region of HIV-1 derived from two chronically HIV-1 infected individuals. The clonal mAbs exhibited a range of reactivities, and the clones with superior properties were associated with the rate of mutations and the presence of particular mutated residues in the light chains, but not in the heavy chains. Our observations suggest that for some antibodies, the light chains play a vital role in antibody evolution toward more efficient ones and also suggest the importance of optimal residues rather than the rate of mutations in the variable fragment of the antibody.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>34340867</pmid><doi>10.1016/j.humimm.2021.05.004</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-7988-2397</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0198-8859 |
| ispartof | Human immunology, 2021-12, Vol.82 (12), p.923-929 |
| issn | 0198-8859 1879-1166 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Antibodies, Monoclonal - genetics Antibodies, Monoclonal - immunology Clonal antibodies Female HIV antibodies HIV Antibodies - genetics HIV Antibodies - immunology HIV Infections - genetics HIV Infections - immunology HIV-1 - genetics HIV-1 - immunology Humans Immunoglobulin Light Chains - genetics Immunoglobulin Light Chains - immunology Immunoglobulin Variable Region - genetics Immunoglobulin Variable Region - immunology Light chain of antibody Male Somatic hypermutations |
| title | The light chain of antibodies specific to the V2 region of HIV-1 can determine their function |
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