How safe are gloves and masks used for protection against Legionella longbeachae infection when gardening?
Legionella longbeachae has been frequently identified in composted plant material and can cause Legionnaires’ disease (LD). We wanted to determine how frequently L. longbeachae DNA was present on gardeners’ gloves, and how long L. longbeachae could persist on inoculated gloves and masks. Volunteers...
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| Veröffentlicht in: | Letters in applied microbiology 2021-11, Vol.73 (5), p.616-622 |
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| creator | Chambers, S.T. Withers, A. Dawson, K. Anderson, T. Williman, J. Murdoch, D. Scott‐Thomas, A. Slow, S. |
| description | Legionella longbeachae has been frequently identified in composted plant material and can cause Legionnaires’ disease (LD). We wanted to determine how frequently L. longbeachae DNA was present on gardeners’ gloves, and how long L. longbeachae could persist on inoculated gloves and masks. Volunteers completed a survey of gardening practices and their gardening gloves were tested for L. longbeachae DNA by qPCR. The persistence of viable L. longbeachae was assessed by timed subcultures after inoculation of gardening gloves and masks. Gloves but not masks were used regularly. L. longbeachae was detected on 11 (14%; 95% CI 8–24%) gloves. Viable organisms were recovered from 25–50% of inoculated cotton, leather and PU coated gloves but not rubber gloves after 8 h incubation. There was a difference in dose‐response curve slopes by glove material (P = 0·001) and time to 50% sterility (P = 0·036). There were differences in persistence of L. longbeachae between mask types from analysis of the slopes and 50% sterility on the decay curves (P = 0·042, P |
| doi_str_mv | 10.1111/lam.13546 |
| format | Article |
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Significance and Impact of the Study: Legionella longbeachae was detectable on 14% of gardeners’ gloves by qPCR. Viable L. longbeachae inoculated onto gloves and masks may persist for several hours to a few days. Contaminated gloves and masks may become a hazard for gardeners and contribute to cases of Legionnaires’ disease.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>DOI: 10.1111/lam.13546</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Cotton ; Deoxyribonucleic acid ; DNA ; Gardening ; Gardens & gardening ; Gloves ; Inoculation ; Leather ; Legionella ; Legionella longbeachae ; Legionnaire's disease ; Legionnaires disease ; Masks ; potting soil ; Risk management ; Slopes ; Sterility</subject><ispartof>Letters in applied microbiology, 2021-11, Vol.73 (5), p.616-622</ispartof><rights>2021 The Society for Applied Microbiology</rights><rights>Copyright © 2021 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3306-e10f164bea2caff24f5626136fc0f9df217d332d766d2352d5e81ad35a35af9c3</citedby><cites>FETCH-LOGICAL-c3306-e10f164bea2caff24f5626136fc0f9df217d332d766d2352d5e81ad35a35af9c3</cites><orcidid>0000-0002-3246-2491 ; 0000-0002-0698-3285</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Flam.13546$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Flam.13546$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Chambers, S.T.</creatorcontrib><creatorcontrib>Withers, A.</creatorcontrib><creatorcontrib>Dawson, K.</creatorcontrib><creatorcontrib>Anderson, T.</creatorcontrib><creatorcontrib>Williman, J.</creatorcontrib><creatorcontrib>Murdoch, D.</creatorcontrib><creatorcontrib>Scott‐Thomas, A.</creatorcontrib><creatorcontrib>Slow, S.</creatorcontrib><title>How safe are gloves and masks used for protection against Legionella longbeachae infection when gardening?</title><title>Letters in applied microbiology</title><description>Legionella longbeachae has been frequently identified in composted plant material and can cause Legionnaires’ disease (LD). We wanted to determine how frequently L. longbeachae DNA was present on gardeners’ gloves, and how long L. longbeachae could persist on inoculated gloves and masks. Volunteers completed a survey of gardening practices and their gardening gloves were tested for L. longbeachae DNA by qPCR. The persistence of viable L. longbeachae was assessed by timed subcultures after inoculation of gardening gloves and masks. Gloves but not masks were used regularly. L. longbeachae was detected on 11 (14%; 95% CI 8–24%) gloves. Viable organisms were recovered from 25–50% of inoculated cotton, leather and PU coated gloves but not rubber gloves after 8 h incubation. There was a difference in dose‐response curve slopes by glove material (P = 0·001) and time to 50% sterility (P = 0·036). There were differences in persistence of L. longbeachae between mask types from analysis of the slopes and 50% sterility on the decay curves (P = 0·042, P < 0·001 respectively). Gardening gloves and masks may act as a vector for transmission of L. longbeachae during gardening. Washing gardening gloves and prompt disposal of masks could reduce risk of LD.
Significance and Impact of the Study: Legionella longbeachae was detectable on 14% of gardeners’ gloves by qPCR. Viable L. longbeachae inoculated onto gloves and masks may persist for several hours to a few days. Contaminated gloves and masks may become a hazard for gardeners and contribute to cases of Legionnaires’ disease.</description><subject>Cotton</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Gardening</subject><subject>Gardens & gardening</subject><subject>Gloves</subject><subject>Inoculation</subject><subject>Leather</subject><subject>Legionella</subject><subject>Legionella longbeachae</subject><subject>Legionnaire's disease</subject><subject>Legionnaires disease</subject><subject>Masks</subject><subject>potting soil</subject><subject>Risk management</subject><subject>Slopes</subject><subject>Sterility</subject><issn>0266-8254</issn><issn>1472-765X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp10E9LwzAYBvAgCs7pwW8Q8KKHbvnf7iRD1AkTLwreSmzedJ1tMpPNsW9vZncSDIHwwi8vDw9Cl5SMaDrjVncjyqVQR2hARc6yXMn3YzQgTKmsYFKcorMYl4SQgrLJAC1nfoujtoB1AFy3_hsi1s7gTsfPiDcRDLY-4FXwa6jWjXdY17pxcY3nUKcR2lbj1rv6A3S10IAbZw9wuwCHax0MuMbVt-foxOo2wsXhHaK3h_vXu1k2f3l8upvOs4pzojKgxFIl0jpWaWuZsFIxRbmyFbETYxnNDefM5EoZxiUzEgqqDZc6XTup-BBd93tT5q8NxHXZNbHa53TgN7FkUuZSiJwUiV79oUu_CS6lS6rIiVA5E0nd9KoKPsYAtlyFptNhV1JS7lsvU-vlb-vJjnu7bVrY_Q_L-fS5__EDpM6EKg</recordid><startdate>202111</startdate><enddate>202111</enddate><creator>Chambers, S.T.</creator><creator>Withers, A.</creator><creator>Dawson, K.</creator><creator>Anderson, T.</creator><creator>Williman, J.</creator><creator>Murdoch, D.</creator><creator>Scott‐Thomas, A.</creator><creator>Slow, S.</creator><general>Oxford University Press</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3246-2491</orcidid><orcidid>https://orcid.org/0000-0002-0698-3285</orcidid></search><sort><creationdate>202111</creationdate><title>How safe are gloves and masks used for protection against Legionella longbeachae infection when gardening?</title><author>Chambers, S.T. ; Withers, A. ; Dawson, K. ; Anderson, T. ; Williman, J. ; Murdoch, D. ; Scott‐Thomas, A. ; Slow, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3306-e10f164bea2caff24f5626136fc0f9df217d332d766d2352d5e81ad35a35af9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Cotton</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Gardening</topic><topic>Gardens & gardening</topic><topic>Gloves</topic><topic>Inoculation</topic><topic>Leather</topic><topic>Legionella</topic><topic>Legionella longbeachae</topic><topic>Legionnaire's disease</topic><topic>Legionnaires disease</topic><topic>Masks</topic><topic>potting soil</topic><topic>Risk management</topic><topic>Slopes</topic><topic>Sterility</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chambers, S.T.</creatorcontrib><creatorcontrib>Withers, A.</creatorcontrib><creatorcontrib>Dawson, K.</creatorcontrib><creatorcontrib>Anderson, T.</creatorcontrib><creatorcontrib>Williman, J.</creatorcontrib><creatorcontrib>Murdoch, D.</creatorcontrib><creatorcontrib>Scott‐Thomas, A.</creatorcontrib><creatorcontrib>Slow, S.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chambers, S.T.</au><au>Withers, A.</au><au>Dawson, K.</au><au>Anderson, T.</au><au>Williman, J.</au><au>Murdoch, D.</au><au>Scott‐Thomas, A.</au><au>Slow, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>How safe are gloves and masks used for protection against Legionella longbeachae infection when gardening?</atitle><jtitle>Letters in applied microbiology</jtitle><date>2021-11</date><risdate>2021</risdate><volume>73</volume><issue>5</issue><spage>616</spage><epage>622</epage><pages>616-622</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><abstract>Legionella longbeachae has been frequently identified in composted plant material and can cause Legionnaires’ disease (LD). We wanted to determine how frequently L. longbeachae DNA was present on gardeners’ gloves, and how long L. longbeachae could persist on inoculated gloves and masks. Volunteers completed a survey of gardening practices and their gardening gloves were tested for L. longbeachae DNA by qPCR. The persistence of viable L. longbeachae was assessed by timed subcultures after inoculation of gardening gloves and masks. Gloves but not masks were used regularly. L. longbeachae was detected on 11 (14%; 95% CI 8–24%) gloves. Viable organisms were recovered from 25–50% of inoculated cotton, leather and PU coated gloves but not rubber gloves after 8 h incubation. There was a difference in dose‐response curve slopes by glove material (P = 0·001) and time to 50% sterility (P = 0·036). There were differences in persistence of L. longbeachae between mask types from analysis of the slopes and 50% sterility on the decay curves (P = 0·042, P < 0·001 respectively). Gardening gloves and masks may act as a vector for transmission of L. longbeachae during gardening. Washing gardening gloves and prompt disposal of masks could reduce risk of LD.
Significance and Impact of the Study: Legionella longbeachae was detectable on 14% of gardeners’ gloves by qPCR. Viable L. longbeachae inoculated onto gloves and masks may persist for several hours to a few days. Contaminated gloves and masks may become a hazard for gardeners and contribute to cases of Legionnaires’ disease.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><doi>10.1111/lam.13546</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-3246-2491</orcidid><orcidid>https://orcid.org/0000-0002-0698-3285</orcidid></addata></record> |
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| ispartof | Letters in applied microbiology, 2021-11, Vol.73 (5), p.616-622 |
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| source | Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Cotton Deoxyribonucleic acid DNA Gardening Gardens & gardening Gloves Inoculation Leather Legionella Legionella longbeachae Legionnaire's disease Legionnaires disease Masks potting soil Risk management Slopes Sterility |
| title | How safe are gloves and masks used for protection against Legionella longbeachae infection when gardening? |
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