MiR‐144/451a cluster synergistically modulates growth and metastasis of Oral Carcinoma
Objectives MicroRNA (miRNA) clusters co‐transcribe and function in a coordinated fashion mediating synergistic or antagonistic regulatory effects. MiR‐144 and miR‐451a are deregulated in various cancers but the combined regulatory role of miR‐144/451a cluster in oral squamous cell carcinoma (OSCC) r...
Gespeichert in:
| Veröffentlicht in: | Oral diseases 2023-03, Vol.29 (2), p.584-594 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 594 |
|---|---|
| container_issue | 2 |
| container_start_page | 584 |
| container_title | Oral diseases |
| container_volume | 29 |
| creator | Manasa, Vidyadharan Geetha Thomas, Shaji Kannan, Sankarareddiar |
| description | Objectives
MicroRNA (miRNA) clusters co‐transcribe and function in a coordinated fashion mediating synergistic or antagonistic regulatory effects. MiR‐144 and miR‐451a are deregulated in various cancers but the combined regulatory role of miR‐144/451a cluster in oral squamous cell carcinoma (OSCC) remains unexplored. In the present study, we studied the synergistic effect of miR‐144/451a cluster on oral cancer progression.
Materials and methods
miR‐144 and miR‐451a expression was explored in OSCC cell lines by quantitative real‐time PCR (qRT‐PCR). Proliferation, wound healing, migration and invasion, spheroid formation, and colony formation assays were performed after transfection with miR‐144‐3p, miR‐451a, miR‐144‐5p, and co‐expressed miR‐144/451a. Expression of putative target genes was analyzed using qRT‐PCR and Western blotting.
Results
miR‐144 and miR‐451a were downregulated in all cell lines. The cell viability and stemness of cancer cell lines were unaltered when treated with miRNA mimics. However, co‐expressed miR‐144/451a significantly reduced the migratory, invasive, and clonogenic potential of cells than individual miRNAs.
Conclusion
miR‐144/451a cluster functions as a tumor suppressor in OSCC by inhibiting cancer cell invasion, migration, and clonogenic potential. |
| doi_str_mv | 10.1111/odi.13984 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2557536645</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2557536645</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3534-e3e145adf30b6bae0d3edd689dd7eff395a1a971123b499ef674f7428fc9dd6c3</originalsourceid><addsrcrecordid>eNp1kMtKJDEUQIMoPmcxPzAE3Oii7KRuUo_l0M5DaGkYHHAX0pUbJ5KqOEkV0rv5BL_RLzEz3boYMFzIXRwOl0PIR84ueH6zYNwFh7YRO-SQV4wXrCnlbt5BikKWcHtAjlK6Z4zXLZT75AAEADRcHpLba_fj-c8TF2ImJNe081MaMdK0HjDeuTS6Tnu_pn0wk9cjJnoXw-P4i-rB0B5HnfK4RIOly6g9nevYuSH0-oTsWe0Tftj-x-Tn1y838-_FYvntav55UXQgQRQIyIXUxgJbVSuNzAAaUzWtMTVaC63UXLc15yWsRNuirWpha1E2tstI1cExOdt4H2L4PWEaVe9Sh97rAcOUVCllLaGqhMzo6X_ofZjikK9TZV2XvMrVWKbON1QXQ0oRrXqIrtdxrThTf3OrnFv9y53ZT1vjtOrRvJGvfTMw2wCPzuP6fZNaXl5tlC_cMInV</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2772168250</pqid></control><display><type>article</type><title>MiR‐144/451a cluster synergistically modulates growth and metastasis of Oral Carcinoma</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Manasa, Vidyadharan Geetha ; Thomas, Shaji ; Kannan, Sankarareddiar</creator><creatorcontrib>Manasa, Vidyadharan Geetha ; Thomas, Shaji ; Kannan, Sankarareddiar</creatorcontrib><description>Objectives
MicroRNA (miRNA) clusters co‐transcribe and function in a coordinated fashion mediating synergistic or antagonistic regulatory effects. MiR‐144 and miR‐451a are deregulated in various cancers but the combined regulatory role of miR‐144/451a cluster in oral squamous cell carcinoma (OSCC) remains unexplored. In the present study, we studied the synergistic effect of miR‐144/451a cluster on oral cancer progression.
Materials and methods
miR‐144 and miR‐451a expression was explored in OSCC cell lines by quantitative real‐time PCR (qRT‐PCR). Proliferation, wound healing, migration and invasion, spheroid formation, and colony formation assays were performed after transfection with miR‐144‐3p, miR‐451a, miR‐144‐5p, and co‐expressed miR‐144/451a. Expression of putative target genes was analyzed using qRT‐PCR and Western blotting.
Results
miR‐144 and miR‐451a were downregulated in all cell lines. The cell viability and stemness of cancer cell lines were unaltered when treated with miRNA mimics. However, co‐expressed miR‐144/451a significantly reduced the migratory, invasive, and clonogenic potential of cells than individual miRNAs.
Conclusion
miR‐144/451a cluster functions as a tumor suppressor in OSCC by inhibiting cancer cell invasion, migration, and clonogenic potential.</description><identifier>ISSN: 1354-523X</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.13984</identifier><identifier>PMID: 34333815</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Cancer ; Carcinoma, Squamous Cell - pathology ; Cell Line, Tumor ; Cell migration ; Cell Movement - genetics ; Cell Proliferation - genetics ; Cell viability ; Gene Expression Regulation, Neoplastic ; Head and Neck Neoplasms - genetics ; Humans ; Metastases ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; MiR‐144 ; MiR‐144/451a cluster ; MiR‐451a ; Mouth Neoplasms - pathology ; Oral cancer ; Oral carcinoma ; Oral squamous cell carcinoma ; Squamous cell carcinoma ; Squamous Cell Carcinoma of Head and Neck - genetics ; Transfection ; Tumor cell lines ; Tumor suppressor genes ; Western blotting ; Wound healing</subject><ispartof>Oral diseases, 2023-03, Vol.29 (2), p.584-594</ispartof><rights>2021 Wiley Periodicals LLC</rights><rights>2021 Wiley Periodicals LLC.</rights><rights>2023 Wiley Periodicals LLC</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3534-e3e145adf30b6bae0d3edd689dd7eff395a1a971123b499ef674f7428fc9dd6c3</citedby><cites>FETCH-LOGICAL-c3534-e3e145adf30b6bae0d3edd689dd7eff395a1a971123b499ef674f7428fc9dd6c3</cites><orcidid>0000-0001-8833-9620</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fodi.13984$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fodi.13984$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34333815$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Manasa, Vidyadharan Geetha</creatorcontrib><creatorcontrib>Thomas, Shaji</creatorcontrib><creatorcontrib>Kannan, Sankarareddiar</creatorcontrib><title>MiR‐144/451a cluster synergistically modulates growth and metastasis of Oral Carcinoma</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Objectives
MicroRNA (miRNA) clusters co‐transcribe and function in a coordinated fashion mediating synergistic or antagonistic regulatory effects. MiR‐144 and miR‐451a are deregulated in various cancers but the combined regulatory role of miR‐144/451a cluster in oral squamous cell carcinoma (OSCC) remains unexplored. In the present study, we studied the synergistic effect of miR‐144/451a cluster on oral cancer progression.
Materials and methods
miR‐144 and miR‐451a expression was explored in OSCC cell lines by quantitative real‐time PCR (qRT‐PCR). Proliferation, wound healing, migration and invasion, spheroid formation, and colony formation assays were performed after transfection with miR‐144‐3p, miR‐451a, miR‐144‐5p, and co‐expressed miR‐144/451a. Expression of putative target genes was analyzed using qRT‐PCR and Western blotting.
Results
miR‐144 and miR‐451a were downregulated in all cell lines. The cell viability and stemness of cancer cell lines were unaltered when treated with miRNA mimics. However, co‐expressed miR‐144/451a significantly reduced the migratory, invasive, and clonogenic potential of cells than individual miRNAs.
Conclusion
miR‐144/451a cluster functions as a tumor suppressor in OSCC by inhibiting cancer cell invasion, migration, and clonogenic potential.</description><subject>Cancer</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement - genetics</subject><subject>Cell Proliferation - genetics</subject><subject>Cell viability</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Head and Neck Neoplasms - genetics</subject><subject>Humans</subject><subject>Metastases</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>MiR‐144</subject><subject>MiR‐144/451a cluster</subject><subject>MiR‐451a</subject><subject>Mouth Neoplasms - pathology</subject><subject>Oral cancer</subject><subject>Oral carcinoma</subject><subject>Oral squamous cell carcinoma</subject><subject>Squamous cell carcinoma</subject><subject>Squamous Cell Carcinoma of Head and Neck - genetics</subject><subject>Transfection</subject><subject>Tumor cell lines</subject><subject>Tumor suppressor genes</subject><subject>Western blotting</subject><subject>Wound healing</subject><issn>1354-523X</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtKJDEUQIMoPmcxPzAE3Oii7KRuUo_l0M5DaGkYHHAX0pUbJ5KqOEkV0rv5BL_RLzEz3boYMFzIXRwOl0PIR84ueH6zYNwFh7YRO-SQV4wXrCnlbt5BikKWcHtAjlK6Z4zXLZT75AAEADRcHpLba_fj-c8TF2ImJNe081MaMdK0HjDeuTS6Tnu_pn0wk9cjJnoXw-P4i-rB0B5HnfK4RIOly6g9nevYuSH0-oTsWe0Tftj-x-Tn1y838-_FYvntav55UXQgQRQIyIXUxgJbVSuNzAAaUzWtMTVaC63UXLc15yWsRNuirWpha1E2tstI1cExOdt4H2L4PWEaVe9Sh97rAcOUVCllLaGqhMzo6X_ofZjikK9TZV2XvMrVWKbON1QXQ0oRrXqIrtdxrThTf3OrnFv9y53ZT1vjtOrRvJGvfTMw2wCPzuP6fZNaXl5tlC_cMInV</recordid><startdate>202303</startdate><enddate>202303</enddate><creator>Manasa, Vidyadharan Geetha</creator><creator>Thomas, Shaji</creator><creator>Kannan, Sankarareddiar</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8833-9620</orcidid></search><sort><creationdate>202303</creationdate><title>MiR‐144/451a cluster synergistically modulates growth and metastasis of Oral Carcinoma</title><author>Manasa, Vidyadharan Geetha ; Thomas, Shaji ; Kannan, Sankarareddiar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3534-e3e145adf30b6bae0d3edd689dd7eff395a1a971123b499ef674f7428fc9dd6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Cancer</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Cell Movement - genetics</topic><topic>Cell Proliferation - genetics</topic><topic>Cell viability</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Head and Neck Neoplasms - genetics</topic><topic>Humans</topic><topic>Metastases</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>MiR‐144</topic><topic>MiR‐144/451a cluster</topic><topic>MiR‐451a</topic><topic>Mouth Neoplasms - pathology</topic><topic>Oral cancer</topic><topic>Oral carcinoma</topic><topic>Oral squamous cell carcinoma</topic><topic>Squamous cell carcinoma</topic><topic>Squamous Cell Carcinoma of Head and Neck - genetics</topic><topic>Transfection</topic><topic>Tumor cell lines</topic><topic>Tumor suppressor genes</topic><topic>Western blotting</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Manasa, Vidyadharan Geetha</creatorcontrib><creatorcontrib>Thomas, Shaji</creatorcontrib><creatorcontrib>Kannan, Sankarareddiar</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Manasa, Vidyadharan Geetha</au><au>Thomas, Shaji</au><au>Kannan, Sankarareddiar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MiR‐144/451a cluster synergistically modulates growth and metastasis of Oral Carcinoma</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2023-03</date><risdate>2023</risdate><volume>29</volume><issue>2</issue><spage>584</spage><epage>594</epage><pages>584-594</pages><issn>1354-523X</issn><eissn>1601-0825</eissn><abstract>Objectives
MicroRNA (miRNA) clusters co‐transcribe and function in a coordinated fashion mediating synergistic or antagonistic regulatory effects. MiR‐144 and miR‐451a are deregulated in various cancers but the combined regulatory role of miR‐144/451a cluster in oral squamous cell carcinoma (OSCC) remains unexplored. In the present study, we studied the synergistic effect of miR‐144/451a cluster on oral cancer progression.
Materials and methods
miR‐144 and miR‐451a expression was explored in OSCC cell lines by quantitative real‐time PCR (qRT‐PCR). Proliferation, wound healing, migration and invasion, spheroid formation, and colony formation assays were performed after transfection with miR‐144‐3p, miR‐451a, miR‐144‐5p, and co‐expressed miR‐144/451a. Expression of putative target genes was analyzed using qRT‐PCR and Western blotting.
Results
miR‐144 and miR‐451a were downregulated in all cell lines. The cell viability and stemness of cancer cell lines were unaltered when treated with miRNA mimics. However, co‐expressed miR‐144/451a significantly reduced the migratory, invasive, and clonogenic potential of cells than individual miRNAs.
Conclusion
miR‐144/451a cluster functions as a tumor suppressor in OSCC by inhibiting cancer cell invasion, migration, and clonogenic potential.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>34333815</pmid><doi>10.1111/odi.13984</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-8833-9620</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1354-523X |
| ispartof | Oral diseases, 2023-03, Vol.29 (2), p.584-594 |
| issn | 1354-523X 1601-0825 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2557536645 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Cancer Carcinoma, Squamous Cell - pathology Cell Line, Tumor Cell migration Cell Movement - genetics Cell Proliferation - genetics Cell viability Gene Expression Regulation, Neoplastic Head and Neck Neoplasms - genetics Humans Metastases MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism miRNA MiR‐144 MiR‐144/451a cluster MiR‐451a Mouth Neoplasms - pathology Oral cancer Oral carcinoma Oral squamous cell carcinoma Squamous cell carcinoma Squamous Cell Carcinoma of Head and Neck - genetics Transfection Tumor cell lines Tumor suppressor genes Western blotting Wound healing |
| title | MiR‐144/451a cluster synergistically modulates growth and metastasis of Oral Carcinoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-19T16%3A29%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=MiR%E2%80%90144/451a%20cluster%20synergistically%20modulates%20growth%20and%20metastasis%20of%20Oral%20Carcinoma&rft.jtitle=Oral%20diseases&rft.au=Manasa,%20Vidyadharan%20Geetha&rft.date=2023-03&rft.volume=29&rft.issue=2&rft.spage=584&rft.epage=594&rft.pages=584-594&rft.issn=1354-523X&rft.eissn=1601-0825&rft_id=info:doi/10.1111/odi.13984&rft_dat=%3Cproquest_cross%3E2557536645%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2772168250&rft_id=info:pmid/34333815&rfr_iscdi=true |