Nutrient load acts as a driver of gut microbiota load, community composition and metabolic functionality in the simulator of the human intestinal microbial ecosystem
ABSTRACT A recently introduced quantitative framework for gut microbiota analysis indicated that microbial load alterations can be linked to various diseases, making it essential to pinpoint its determinants. We identified nutrient load as a main driver of the quantitative microbial community compos...
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| Veröffentlicht in: | FEMS microbiology ecology 2021-09, Vol.97 (9) |
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| creator | Minnebo, Yorick De Paepe, Kim Raes, Jeroen de Wiele, Tom Van |
| description | ABSTRACT
A recently introduced quantitative framework for gut microbiota analysis indicated that microbial load alterations can be linked to various diseases, making it essential to pinpoint its determinants. We identified nutrient load as a main driver of the quantitative microbial community composition and functionality in vitro by stepwise decreasing standardized feed concentrations from 100% to 33%, 20% and 10% in 5-day intervals. While the proportional composition and metabolic profile were mainly determined by the inter-individual variability (35% and 41%), nutrient load accounted for 58%, 23% and 65% of the observed variation in the microbial load, quantitative composition and net daily metabolite production, respectively. After the 10-fold nutrient reduction, the microbial load decreased by 79.72 ± 9% and 82.96 ± 1.66% in the proximal and distal colon, respectively, while the net total short-chain fatty acid production dropped by 79.42 ± 4.42% and 84.58 ± 2.42%, respectively. The majority of microbial taxa quantitatively decreased, whereas a select group of nutritional specialists, such as Akkermansia muciniphila and Bilophila wadsworthia, and a number of opportunistic pathogens remained unaffected. This shows that nutrient load is an important driver of the human gut microbiome and should be considered in future in vitro and in vivo dietary research.
This study, performed in an advanced in vitro gastrointestinal model, identified nutrient load as an important driver of the gut microbial load, quantitative gut microbiome composition and metabolic functionality. |
| doi_str_mv | 10.1093/femsec/fiab111 |
| format | Article |
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A recently introduced quantitative framework for gut microbiota analysis indicated that microbial load alterations can be linked to various diseases, making it essential to pinpoint its determinants. We identified nutrient load as a main driver of the quantitative microbial community composition and functionality in vitro by stepwise decreasing standardized feed concentrations from 100% to 33%, 20% and 10% in 5-day intervals. While the proportional composition and metabolic profile were mainly determined by the inter-individual variability (35% and 41%), nutrient load accounted for 58%, 23% and 65% of the observed variation in the microbial load, quantitative composition and net daily metabolite production, respectively. After the 10-fold nutrient reduction, the microbial load decreased by 79.72 ± 9% and 82.96 ± 1.66% in the proximal and distal colon, respectively, while the net total short-chain fatty acid production dropped by 79.42 ± 4.42% and 84.58 ± 2.42%, respectively. The majority of microbial taxa quantitatively decreased, whereas a select group of nutritional specialists, such as Akkermansia muciniphila and Bilophila wadsworthia, and a number of opportunistic pathogens remained unaffected. This shows that nutrient load is an important driver of the human gut microbiome and should be considered in future in vitro and in vivo dietary research.
This study, performed in an advanced in vitro gastrointestinal model, identified nutrient load as an important driver of the gut microbial load, quantitative gut microbiome composition and metabolic functionality.</description><identifier>ISSN: 1574-6941</identifier><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1093/femsec/fiab111</identifier><language>eng</language><publisher>Delft: Oxford University Press</publisher><subject>Acid production ; Colon ; Community composition ; Composition ; Digestive system ; Ecology ; Fatty acids ; Gastrointestinal tract ; Intestinal microflora ; Metabolism ; Metabolites ; Microbiology ; Microbiomes ; Microbiota ; Microorganisms ; Nutrient loading ; Opportunist infection</subject><ispartof>FEMS microbiology ecology, 2021-09, Vol.97 (9)</ispartof><rights>The Author(s) 2021. Published by Oxford University Press on behalf of FEMS. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2021</rights><rights>The Author(s) 2021. Published by Oxford University Press on behalf of FEMS. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-b14d2972e7264e852baaafeaaaa5f07fafa112d1c2d7f74f992d9978ba78e4ac3</citedby><cites>FETCH-LOGICAL-c374t-b14d2972e7264e852baaafeaaaa5f07fafa112d1c2d7f74f992d9978ba78e4ac3</cites><orcidid>0000-0002-1980-7117</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1604,27924,27925</link.rule.ids><linktorsrc>$$Uhttps://dx.doi.org/10.1093/femsec/fiab111$$EView_record_in_Oxford_University_Press$$FView_record_in_$$GOxford_University_Press</linktorsrc></links><search><creatorcontrib>Minnebo, Yorick</creatorcontrib><creatorcontrib>De Paepe, Kim</creatorcontrib><creatorcontrib>Raes, Jeroen</creatorcontrib><creatorcontrib>de Wiele, Tom Van</creatorcontrib><title>Nutrient load acts as a driver of gut microbiota load, community composition and metabolic functionality in the simulator of the human intestinal microbial ecosystem</title><title>FEMS microbiology ecology</title><description>ABSTRACT
A recently introduced quantitative framework for gut microbiota analysis indicated that microbial load alterations can be linked to various diseases, making it essential to pinpoint its determinants. We identified nutrient load as a main driver of the quantitative microbial community composition and functionality in vitro by stepwise decreasing standardized feed concentrations from 100% to 33%, 20% and 10% in 5-day intervals. While the proportional composition and metabolic profile were mainly determined by the inter-individual variability (35% and 41%), nutrient load accounted for 58%, 23% and 65% of the observed variation in the microbial load, quantitative composition and net daily metabolite production, respectively. After the 10-fold nutrient reduction, the microbial load decreased by 79.72 ± 9% and 82.96 ± 1.66% in the proximal and distal colon, respectively, while the net total short-chain fatty acid production dropped by 79.42 ± 4.42% and 84.58 ± 2.42%, respectively. The majority of microbial taxa quantitatively decreased, whereas a select group of nutritional specialists, such as Akkermansia muciniphila and Bilophila wadsworthia, and a number of opportunistic pathogens remained unaffected. This shows that nutrient load is an important driver of the human gut microbiome and should be considered in future in vitro and in vivo dietary research.
This study, performed in an advanced in vitro gastrointestinal model, identified nutrient load as an important driver of the gut microbial load, quantitative gut microbiome composition and metabolic functionality.</description><subject>Acid production</subject><subject>Colon</subject><subject>Community composition</subject><subject>Composition</subject><subject>Digestive system</subject><subject>Ecology</subject><subject>Fatty acids</subject><subject>Gastrointestinal tract</subject><subject>Intestinal microflora</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Microbiology</subject><subject>Microbiomes</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Nutrient loading</subject><subject>Opportunist infection</subject><issn>1574-6941</issn><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1LAzEQhhdRUKtXzwEvCrbdZLNN9yjiFxS96HmZzU5sZLOp-RD6g_yfZtsK4kUIycvMM5MZ3iw7o_mE5lUxVWg8yqnS0FBK97IjWgo-nlWc7v_Sh9mx9-95TsuC50fZ11MMTmMfSGehJSCDJ5AOaZ3-REesIm8xEKOls422ATbcFZHWmNjrsB7UynodtO0J9C0xGKCxnZZExV4OYegGTvckLJF4bWIHwW5aD4FlNNCnbEAfdGJ__koKpfVrH9CcZAcKOo-nu3eUvd7dvtw8jBfP948314uxLAQP44byllWCoWAzjvOSNQCgMF1QqlwoUEApa6lkrVCCq6pibVWJeQNijhxkMcoutn1Xzn7ENFBttJfYddCjjb5mZTkr5jNOi4Se_0HfbXRp_oESrMgTJRI12VJpJe8dqnrltAG3rmleD67VW9fqnWup4HJbYOPqP_YbEuGgKQ</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Minnebo, Yorick</creator><creator>De Paepe, Kim</creator><creator>Raes, Jeroen</creator><creator>de Wiele, Tom Van</creator><general>Oxford University Press</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1980-7117</orcidid></search><sort><creationdate>20210901</creationdate><title>Nutrient load acts as a driver of gut microbiota load, community composition and metabolic functionality in the simulator of the human intestinal microbial ecosystem</title><author>Minnebo, Yorick ; 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A recently introduced quantitative framework for gut microbiota analysis indicated that microbial load alterations can be linked to various diseases, making it essential to pinpoint its determinants. We identified nutrient load as a main driver of the quantitative microbial community composition and functionality in vitro by stepwise decreasing standardized feed concentrations from 100% to 33%, 20% and 10% in 5-day intervals. While the proportional composition and metabolic profile were mainly determined by the inter-individual variability (35% and 41%), nutrient load accounted for 58%, 23% and 65% of the observed variation in the microbial load, quantitative composition and net daily metabolite production, respectively. After the 10-fold nutrient reduction, the microbial load decreased by 79.72 ± 9% and 82.96 ± 1.66% in the proximal and distal colon, respectively, while the net total short-chain fatty acid production dropped by 79.42 ± 4.42% and 84.58 ± 2.42%, respectively. The majority of microbial taxa quantitatively decreased, whereas a select group of nutritional specialists, such as Akkermansia muciniphila and Bilophila wadsworthia, and a number of opportunistic pathogens remained unaffected. This shows that nutrient load is an important driver of the human gut microbiome and should be considered in future in vitro and in vivo dietary research.
This study, performed in an advanced in vitro gastrointestinal model, identified nutrient load as an important driver of the gut microbial load, quantitative gut microbiome composition and metabolic functionality.</abstract><cop>Delft</cop><pub>Oxford University Press</pub><doi>10.1093/femsec/fiab111</doi><orcidid>https://orcid.org/0000-0002-1980-7117</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acid production Colon Community composition Composition Digestive system Ecology Fatty acids Gastrointestinal tract Intestinal microflora Metabolism Metabolites Microbiology Microbiomes Microbiota Microorganisms Nutrient loading Opportunist infection |
| title | Nutrient load acts as a driver of gut microbiota load, community composition and metabolic functionality in the simulator of the human intestinal microbial ecosystem |
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