MicroRNA-802 promotes the progression of osteosarcoma through targeting p27 and activating PI3K/AKT pathway
Purpose Increasing evidences suggest dysfunctions of microRNAs (miRNAs) are playing important part in tumors. Therefore, the role of miR-802 in osteosarcoma (OS) was exploited. The object was to evaluate the effect of miR-802 and verify its influence on p27 Kip1 (p27) in OS. Methods RT-qPCR experime...
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| Veröffentlicht in: | Clinical & translational oncology 2022-02, Vol.24 (2), p.266-275 |
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| creator | Gao, L. F. Jia, S. Zhang, Q. M. Xia, Y. F. Li, C. J. Li, Y. H. |
| description | Purpose
Increasing evidences suggest dysfunctions of microRNAs (miRNAs) are playing important part in tumors. Therefore, the role of miR-802 in osteosarcoma (OS) was exploited. The object was to evaluate the effect of miR-802 and verify its influence on p27 Kip1 (p27) in OS.
Methods
RT-qPCR experiment was used to detect miR-802 and p27 expression in OS tissues and cells. We explored the function of miR-802 through Transwell assays. The phosphoinositide 3-kinase (PI3K)/AKT serine/threonine kinase pathway and epithelial–mesenchymal transition (EMT) was detected by Western blot assays. Luciferase assay was used to testify the target of miR-802.
Results
MiR-802 expression was elevated in OS, which was related to poor clinical outcome in OS patients. MiR-802 overexpression promoted OS migration, invasion and EMT. Further, p27 is a direct target of miR-802. P27 elevation counteracted the promotion effect of OS on EMT, migration and invasion induced by miR-802. In addition, miR-802 overexpression inactivated PI3K/AKT pathway via targeting p27 in OS.
Conclusion
MiR-802 promoted the progress of EMT, migration and invasion in OS via targeting p27. This newly identified miR-802/p27/PI3K/AKT axis may represent potential targets for OS. |
| doi_str_mv | 10.1007/s12094-021-02683-w |
| format | Article |
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Increasing evidences suggest dysfunctions of microRNAs (miRNAs) are playing important part in tumors. Therefore, the role of miR-802 in osteosarcoma (OS) was exploited. The object was to evaluate the effect of miR-802 and verify its influence on p27 Kip1 (p27) in OS.
Methods
RT-qPCR experiment was used to detect miR-802 and p27 expression in OS tissues and cells. We explored the function of miR-802 through Transwell assays. The phosphoinositide 3-kinase (PI3K)/AKT serine/threonine kinase pathway and epithelial–mesenchymal transition (EMT) was detected by Western blot assays. Luciferase assay was used to testify the target of miR-802.
Results
MiR-802 expression was elevated in OS, which was related to poor clinical outcome in OS patients. MiR-802 overexpression promoted OS migration, invasion and EMT. Further, p27 is a direct target of miR-802. P27 elevation counteracted the promotion effect of OS on EMT, migration and invasion induced by miR-802. In addition, miR-802 overexpression inactivated PI3K/AKT pathway via targeting p27 in OS.
Conclusion
MiR-802 promoted the progress of EMT, migration and invasion in OS via targeting p27. This newly identified miR-802/p27/PI3K/AKT axis may represent potential targets for OS.</description><identifier>ISSN: 1699-048X</identifier><identifier>EISSN: 1699-3055</identifier><identifier>DOI: 10.1007/s12094-021-02683-w</identifier><identifier>PMID: 34318428</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Adolescent ; Bone Neoplasms - etiology ; Bone Neoplasms - pathology ; Cyclin-Dependent Kinase Inhibitor p27 - physiology ; Disease Progression ; Female ; Humans ; Male ; Medicine ; Medicine & Public Health ; MicroRNAs - physiology ; Oncology ; Osteosarcoma - etiology ; Osteosarcoma - pathology ; Phosphatidylinositol 3-Kinases - physiology ; Proto-Oncogene Proteins c-akt - physiology ; Research Article ; Young Adult</subject><ispartof>Clinical & translational oncology, 2022-02, Vol.24 (2), p.266-275</ispartof><rights>Federación de Sociedades Españolas de Oncología (FESEO) 2021</rights><rights>2021. Federación de Sociedades Españolas de Oncología (FESEO).</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-36704c5e77d53b1df5e6dfcd2d3fc4520a36859913018076f8d4c510bb0dc5b13</citedby><cites>FETCH-LOGICAL-c347t-36704c5e77d53b1df5e6dfcd2d3fc4520a36859913018076f8d4c510bb0dc5b13</cites><orcidid>0000-0002-0819-7827</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12094-021-02683-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12094-021-02683-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34318428$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, L. F.</creatorcontrib><creatorcontrib>Jia, S.</creatorcontrib><creatorcontrib>Zhang, Q. M.</creatorcontrib><creatorcontrib>Xia, Y. F.</creatorcontrib><creatorcontrib>Li, C. J.</creatorcontrib><creatorcontrib>Li, Y. H.</creatorcontrib><title>MicroRNA-802 promotes the progression of osteosarcoma through targeting p27 and activating PI3K/AKT pathway</title><title>Clinical & translational oncology</title><addtitle>Clin Transl Oncol</addtitle><addtitle>Clin Transl Oncol</addtitle><description>Purpose
Increasing evidences suggest dysfunctions of microRNAs (miRNAs) are playing important part in tumors. Therefore, the role of miR-802 in osteosarcoma (OS) was exploited. The object was to evaluate the effect of miR-802 and verify its influence on p27 Kip1 (p27) in OS.
Methods
RT-qPCR experiment was used to detect miR-802 and p27 expression in OS tissues and cells. We explored the function of miR-802 through Transwell assays. The phosphoinositide 3-kinase (PI3K)/AKT serine/threonine kinase pathway and epithelial–mesenchymal transition (EMT) was detected by Western blot assays. Luciferase assay was used to testify the target of miR-802.
Results
MiR-802 expression was elevated in OS, which was related to poor clinical outcome in OS patients. MiR-802 overexpression promoted OS migration, invasion and EMT. Further, p27 is a direct target of miR-802. P27 elevation counteracted the promotion effect of OS on EMT, migration and invasion induced by miR-802. In addition, miR-802 overexpression inactivated PI3K/AKT pathway via targeting p27 in OS.
Conclusion
MiR-802 promoted the progress of EMT, migration and invasion in OS via targeting p27. This newly identified miR-802/p27/PI3K/AKT axis may represent potential targets for OS.</description><subject>Adolescent</subject><subject>Bone Neoplasms - etiology</subject><subject>Bone Neoplasms - pathology</subject><subject>Cyclin-Dependent Kinase Inhibitor p27 - physiology</subject><subject>Disease Progression</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>MicroRNAs - physiology</subject><subject>Oncology</subject><subject>Osteosarcoma - etiology</subject><subject>Osteosarcoma - pathology</subject><subject>Phosphatidylinositol 3-Kinases - physiology</subject><subject>Proto-Oncogene Proteins c-akt - physiology</subject><subject>Research Article</subject><subject>Young Adult</subject><issn>1699-048X</issn><issn>1699-3055</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOxCAUhonReH8BF4alm-oBCm2XE-MtXmM0cUco0E51WkagTnx70RlduiDAOd_5k_MhdEDgmAAUJ4FQqPIMKElHlCxbrKFtIqoqY8D5-uoNefmyhXZCeIVUFYRsoi2WM1LmtNxGb7ed9u7xbpKVQPHcu95FG3Cc2u9P620InRuwa7AL0bqgvHa9Sn3vxnaKo_Ktjd3Q4jktsBoMVjp2H-qn9HDFrk8m1094ruJ0oT730EajZsHur-5d9Hx-9nR6md3cX1ydTm4yzfIiZkwUkGtui8JwVhPTcCtMow01rNE5p6CYKHlVEQakhEI0pUk4gboGo3lN2C46WuamDd5HG6Lsu6DtbKYG68YgKee8EkUlIKF0iSYJIXjbyLnveuU_JQH5LVkuJcskWf5Ilos0dLjKH-vemr-RX6sJYEsgpNbQWi9f3eiHtPN_sV99mIf6</recordid><startdate>20220201</startdate><enddate>20220201</enddate><creator>Gao, L. F.</creator><creator>Jia, S.</creator><creator>Zhang, Q. M.</creator><creator>Xia, Y. F.</creator><creator>Li, C. J.</creator><creator>Li, Y. H.</creator><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0819-7827</orcidid></search><sort><creationdate>20220201</creationdate><title>MicroRNA-802 promotes the progression of osteosarcoma through targeting p27 and activating PI3K/AKT pathway</title><author>Gao, L. F. ; Jia, S. ; Zhang, Q. M. ; Xia, Y. F. ; Li, C. J. ; Li, Y. H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-36704c5e77d53b1df5e6dfcd2d3fc4520a36859913018076f8d4c510bb0dc5b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adolescent</topic><topic>Bone Neoplasms - etiology</topic><topic>Bone Neoplasms - pathology</topic><topic>Cyclin-Dependent Kinase Inhibitor p27 - physiology</topic><topic>Disease Progression</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>MicroRNAs - physiology</topic><topic>Oncology</topic><topic>Osteosarcoma - etiology</topic><topic>Osteosarcoma - pathology</topic><topic>Phosphatidylinositol 3-Kinases - physiology</topic><topic>Proto-Oncogene Proteins c-akt - physiology</topic><topic>Research Article</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, L. F.</creatorcontrib><creatorcontrib>Jia, S.</creatorcontrib><creatorcontrib>Zhang, Q. M.</creatorcontrib><creatorcontrib>Xia, Y. F.</creatorcontrib><creatorcontrib>Li, C. J.</creatorcontrib><creatorcontrib>Li, Y. H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical & translational oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, L. F.</au><au>Jia, S.</au><au>Zhang, Q. M.</au><au>Xia, Y. F.</au><au>Li, C. J.</au><au>Li, Y. H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA-802 promotes the progression of osteosarcoma through targeting p27 and activating PI3K/AKT pathway</atitle><jtitle>Clinical & translational oncology</jtitle><stitle>Clin Transl Oncol</stitle><addtitle>Clin Transl Oncol</addtitle><date>2022-02-01</date><risdate>2022</risdate><volume>24</volume><issue>2</issue><spage>266</spage><epage>275</epage><pages>266-275</pages><issn>1699-048X</issn><eissn>1699-3055</eissn><abstract>Purpose
Increasing evidences suggest dysfunctions of microRNAs (miRNAs) are playing important part in tumors. Therefore, the role of miR-802 in osteosarcoma (OS) was exploited. The object was to evaluate the effect of miR-802 and verify its influence on p27 Kip1 (p27) in OS.
Methods
RT-qPCR experiment was used to detect miR-802 and p27 expression in OS tissues and cells. We explored the function of miR-802 through Transwell assays. The phosphoinositide 3-kinase (PI3K)/AKT serine/threonine kinase pathway and epithelial–mesenchymal transition (EMT) was detected by Western blot assays. Luciferase assay was used to testify the target of miR-802.
Results
MiR-802 expression was elevated in OS, which was related to poor clinical outcome in OS patients. MiR-802 overexpression promoted OS migration, invasion and EMT. Further, p27 is a direct target of miR-802. P27 elevation counteracted the promotion effect of OS on EMT, migration and invasion induced by miR-802. In addition, miR-802 overexpression inactivated PI3K/AKT pathway via targeting p27 in OS.
Conclusion
MiR-802 promoted the progress of EMT, migration and invasion in OS via targeting p27. This newly identified miR-802/p27/PI3K/AKT axis may represent potential targets for OS.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>34318428</pmid><doi>10.1007/s12094-021-02683-w</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-0819-7827</orcidid></addata></record> |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adolescent Bone Neoplasms - etiology Bone Neoplasms - pathology Cyclin-Dependent Kinase Inhibitor p27 - physiology Disease Progression Female Humans Male Medicine Medicine & Public Health MicroRNAs - physiology Oncology Osteosarcoma - etiology Osteosarcoma - pathology Phosphatidylinositol 3-Kinases - physiology Proto-Oncogene Proteins c-akt - physiology Research Article Young Adult |
| title | MicroRNA-802 promotes the progression of osteosarcoma through targeting p27 and activating PI3K/AKT pathway |
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