Identification and molecular characterization of H9N2 viruses carrying multiple mammalian adaptation markers in resident birds in central-western wetlands in India
We report here a targeted risk-based study to investigate the presence of influenza A viruses at the migratory-wild-domestic bird interface across the major wetlands of central India's Maharashtra state during the winter migration season. The H9N2 viruses have been isolated and confirmed in 3.8...
Gespeichert in:
Veröffentlicht in: | Infection, genetics and evolution genetics and evolution, 2021-10, Vol.94, p.105005-105005, Article 105005 |
---|---|
Hauptverfasser: | , , , , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 105005 |
---|---|
container_issue | |
container_start_page | 105005 |
container_title | Infection, genetics and evolution |
container_volume | 94 |
creator | Sood, Richa Kumar, Naveen Gokhe, Suresh S. Pateriya, Atul Kumar Bhat, Sushant Bhatia, Sandeep Panickan, Sivasankar Mishra, Anamika Murugkar, H.V. Dixit, Roma Shrivastava, Deepali Singh, Pushpendra Tripathi, Meghna Singh, Vijendra Pal |
description | We report here a targeted risk-based study to investigate the presence of influenza A viruses at the migratory-wild-domestic bird interface across the major wetlands of central India's Maharashtra state during the winter migration season. The H9N2 viruses have been isolated and confirmed in 3.86% (33/854) of the fecal samples of resident birds. To investigate the genetic pools of H9N2 circulating in resident birds, we sequenced two isolates of H9N2 from distant wetlands. Sequence and phylogenetic analyses have shown that these viruses are triple reassortants, with HA, NA, NP, and M genes belonging to G1 sub-lineage (A/quail/Hong Kong/G1/1997), PB2, PB1, and NS genes originating from the prototype Eurasian lineage (A/mallard/France/090360/2009) and PA gene deriving from Y439/Korean-like (A/duck/Hong Kong/Y439/97) sub-lineage. It was confirmed not only that four of their gene segments had a high genetic association with the zoonotic H9N2 virus, A/Human/India/TCM2581/2019, but also that they had many molecular markers associated with mammalian adaptation and enhanced virulence in mammals including the unique multiple basic amino acids, KSKR↓GLF at the HA cleavage site, and analog N-and O-glycosylation patterns on HA with that of the zoonotic H9N2 virus. Furthermore, future experiments would be to characterize these isolates biologically to address the public health concern. Importantly, due to the identification of these viruses at a strategic geographical location in India (a major stop-over point in the Central Asian flyway), these novel viruses also pose a possible threat to be exported to other regions via migratory/resident birds. Consequently, systematic investigation and active monitoring are a prerequisite for identifying and preventing the spread of viruses of zoonotic potential by enforcing strict biosecurity measures.
•First influenza H9N2 virus study at migratory-wild-domestic bird interface from India.•Triple reassortants H9N2 viruses were isolated from major wetlands of central India.•H9N2 viruses contained many markers for virulence and adaptation in mammalian hosts.•Strong negative purifying selection governs evolution of the Indian H9N2 viruses.•Glycosylation patterns on hemagglutinin match with that of the zoonotic H9N2 virus. |
doi_str_mv | 10.1016/j.meegid.2021.105005 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2555113664</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1567134821003038</els_id><sourcerecordid>2555113664</sourcerecordid><originalsourceid>FETCH-LOGICAL-c362t-d1365f439b3b6fe4d856052be64233aeef5cbc5ff6c2cf653704b1b2b08465c13</originalsourceid><addsrcrecordid>eNp9UctuFDEQHCEQCYE_QMhHLrP4PbsXJBQFslIULsnZ8qMdehnPLPZMovA7-VG8mYUjJ7erq7uqVU3zntEVo0x_2q0SwB2GFaecVUhRql40p0zpru246l4eaybk-qR5U8qOUtZRvn7dnAjJNxVmp83TNsAwYURvJxwHYodA0tiDn3ubif9hs_UTZPy9tMdILjfXnNxjngsU4m3OjzjckTT3E-57IMmmZHu0dVWw-2kZSzb_hFwIDiRDwYMkcZjDM-LrL9u-fYBSlQbyAFNfbTz3tkNA-7Z5FW1f4N3xPWtuv17cnF-2V9-_bc-_XLVeaD61gQmtohQbJ5yOIMNaaaq4Ay25EBYgKu-8ilF77qNWoqPSMccdXUutPBNnzcdl7z6Pv-bqxiQsHvrqBsa5GK6UYlVEy0qVC9XnsZQM0ewz1isfDaPmEI_ZmSUec4jHLPHUsQ9HhdklCP-G_uZRCZ8XAtQ77xGyKR5h8BAwg59MGPH_Cn8Awc6mlw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2555113664</pqid></control><display><type>article</type><title>Identification and molecular characterization of H9N2 viruses carrying multiple mammalian adaptation markers in resident birds in central-western wetlands in India</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Sood, Richa ; Kumar, Naveen ; Gokhe, Suresh S. ; Pateriya, Atul Kumar ; Bhat, Sushant ; Bhatia, Sandeep ; Panickan, Sivasankar ; Mishra, Anamika ; Murugkar, H.V. ; Dixit, Roma ; Shrivastava, Deepali ; Singh, Pushpendra ; Tripathi, Meghna ; Singh, Vijendra Pal</creator><creatorcontrib>Sood, Richa ; Kumar, Naveen ; Gokhe, Suresh S. ; Pateriya, Atul Kumar ; Bhat, Sushant ; Bhatia, Sandeep ; Panickan, Sivasankar ; Mishra, Anamika ; Murugkar, H.V. ; Dixit, Roma ; Shrivastava, Deepali ; Singh, Pushpendra ; Tripathi, Meghna ; Singh, Vijendra Pal</creatorcontrib><description>We report here a targeted risk-based study to investigate the presence of influenza A viruses at the migratory-wild-domestic bird interface across the major wetlands of central India's Maharashtra state during the winter migration season. The H9N2 viruses have been isolated and confirmed in 3.86% (33/854) of the fecal samples of resident birds. To investigate the genetic pools of H9N2 circulating in resident birds, we sequenced two isolates of H9N2 from distant wetlands. Sequence and phylogenetic analyses have shown that these viruses are triple reassortants, with HA, NA, NP, and M genes belonging to G1 sub-lineage (A/quail/Hong Kong/G1/1997), PB2, PB1, and NS genes originating from the prototype Eurasian lineage (A/mallard/France/090360/2009) and PA gene deriving from Y439/Korean-like (A/duck/Hong Kong/Y439/97) sub-lineage. It was confirmed not only that four of their gene segments had a high genetic association with the zoonotic H9N2 virus, A/Human/India/TCM2581/2019, but also that they had many molecular markers associated with mammalian adaptation and enhanced virulence in mammals including the unique multiple basic amino acids, KSKR↓GLF at the HA cleavage site, and analog N-and O-glycosylation patterns on HA with that of the zoonotic H9N2 virus. Furthermore, future experiments would be to characterize these isolates biologically to address the public health concern. Importantly, due to the identification of these viruses at a strategic geographical location in India (a major stop-over point in the Central Asian flyway), these novel viruses also pose a possible threat to be exported to other regions via migratory/resident birds. Consequently, systematic investigation and active monitoring are a prerequisite for identifying and preventing the spread of viruses of zoonotic potential by enforcing strict biosecurity measures.
•First influenza H9N2 virus study at migratory-wild-domestic bird interface from India.•Triple reassortants H9N2 viruses were isolated from major wetlands of central India.•H9N2 viruses contained many markers for virulence and adaptation in mammalian hosts.•Strong negative purifying selection governs evolution of the Indian H9N2 viruses.•Glycosylation patterns on hemagglutinin match with that of the zoonotic H9N2 virus.</description><identifier>ISSN: 1567-1348</identifier><identifier>EISSN: 1567-7257</identifier><identifier>DOI: 10.1016/j.meegid.2021.105005</identifier><identifier>PMID: 34293481</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adaptation, Biological ; Animals ; Biosecurity ; Birds ; H9N2 viruses ; India ; India - epidemiology ; Influenza A Virus, H9N2 Subtype - genetics ; Influenza in Birds - epidemiology ; Influenza in Birds - virology ; Mammals ; Prevalence ; Resident birds ; Wetlands ; Zoonotic potential</subject><ispartof>Infection, genetics and evolution, 2021-10, Vol.94, p.105005-105005, Article 105005</ispartof><rights>2021 Elsevier B.V.</rights><rights>Copyright © 2021 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-d1365f439b3b6fe4d856052be64233aeef5cbc5ff6c2cf653704b1b2b08465c13</citedby><cites>FETCH-LOGICAL-c362t-d1365f439b3b6fe4d856052be64233aeef5cbc5ff6c2cf653704b1b2b08465c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.meegid.2021.105005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34293481$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sood, Richa</creatorcontrib><creatorcontrib>Kumar, Naveen</creatorcontrib><creatorcontrib>Gokhe, Suresh S.</creatorcontrib><creatorcontrib>Pateriya, Atul Kumar</creatorcontrib><creatorcontrib>Bhat, Sushant</creatorcontrib><creatorcontrib>Bhatia, Sandeep</creatorcontrib><creatorcontrib>Panickan, Sivasankar</creatorcontrib><creatorcontrib>Mishra, Anamika</creatorcontrib><creatorcontrib>Murugkar, H.V.</creatorcontrib><creatorcontrib>Dixit, Roma</creatorcontrib><creatorcontrib>Shrivastava, Deepali</creatorcontrib><creatorcontrib>Singh, Pushpendra</creatorcontrib><creatorcontrib>Tripathi, Meghna</creatorcontrib><creatorcontrib>Singh, Vijendra Pal</creatorcontrib><title>Identification and molecular characterization of H9N2 viruses carrying multiple mammalian adaptation markers in resident birds in central-western wetlands in India</title><title>Infection, genetics and evolution</title><addtitle>Infect Genet Evol</addtitle><description>We report here a targeted risk-based study to investigate the presence of influenza A viruses at the migratory-wild-domestic bird interface across the major wetlands of central India's Maharashtra state during the winter migration season. The H9N2 viruses have been isolated and confirmed in 3.86% (33/854) of the fecal samples of resident birds. To investigate the genetic pools of H9N2 circulating in resident birds, we sequenced two isolates of H9N2 from distant wetlands. Sequence and phylogenetic analyses have shown that these viruses are triple reassortants, with HA, NA, NP, and M genes belonging to G1 sub-lineage (A/quail/Hong Kong/G1/1997), PB2, PB1, and NS genes originating from the prototype Eurasian lineage (A/mallard/France/090360/2009) and PA gene deriving from Y439/Korean-like (A/duck/Hong Kong/Y439/97) sub-lineage. It was confirmed not only that four of their gene segments had a high genetic association with the zoonotic H9N2 virus, A/Human/India/TCM2581/2019, but also that they had many molecular markers associated with mammalian adaptation and enhanced virulence in mammals including the unique multiple basic amino acids, KSKR↓GLF at the HA cleavage site, and analog N-and O-glycosylation patterns on HA with that of the zoonotic H9N2 virus. Furthermore, future experiments would be to characterize these isolates biologically to address the public health concern. Importantly, due to the identification of these viruses at a strategic geographical location in India (a major stop-over point in the Central Asian flyway), these novel viruses also pose a possible threat to be exported to other regions via migratory/resident birds. Consequently, systematic investigation and active monitoring are a prerequisite for identifying and preventing the spread of viruses of zoonotic potential by enforcing strict biosecurity measures.
•First influenza H9N2 virus study at migratory-wild-domestic bird interface from India.•Triple reassortants H9N2 viruses were isolated from major wetlands of central India.•H9N2 viruses contained many markers for virulence and adaptation in mammalian hosts.•Strong negative purifying selection governs evolution of the Indian H9N2 viruses.•Glycosylation patterns on hemagglutinin match with that of the zoonotic H9N2 virus.</description><subject>Adaptation, Biological</subject><subject>Animals</subject><subject>Biosecurity</subject><subject>Birds</subject><subject>H9N2 viruses</subject><subject>India</subject><subject>India - epidemiology</subject><subject>Influenza A Virus, H9N2 Subtype - genetics</subject><subject>Influenza in Birds - epidemiology</subject><subject>Influenza in Birds - virology</subject><subject>Mammals</subject><subject>Prevalence</subject><subject>Resident birds</subject><subject>Wetlands</subject><subject>Zoonotic potential</subject><issn>1567-1348</issn><issn>1567-7257</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UctuFDEQHCEQCYE_QMhHLrP4PbsXJBQFslIULsnZ8qMdehnPLPZMovA7-VG8mYUjJ7erq7uqVU3zntEVo0x_2q0SwB2GFaecVUhRql40p0zpru246l4eaybk-qR5U8qOUtZRvn7dnAjJNxVmp83TNsAwYURvJxwHYodA0tiDn3ubif9hs_UTZPy9tMdILjfXnNxjngsU4m3OjzjckTT3E-57IMmmZHu0dVWw-2kZSzb_hFwIDiRDwYMkcZjDM-LrL9u-fYBSlQbyAFNfbTz3tkNA-7Z5FW1f4N3xPWtuv17cnF-2V9-_bc-_XLVeaD61gQmtohQbJ5yOIMNaaaq4Ay25EBYgKu-8ilF77qNWoqPSMccdXUutPBNnzcdl7z6Pv-bqxiQsHvrqBsa5GK6UYlVEy0qVC9XnsZQM0ewz1isfDaPmEI_ZmSUec4jHLPHUsQ9HhdklCP-G_uZRCZ8XAtQ77xGyKR5h8BAwg59MGPH_Cn8Awc6mlw</recordid><startdate>202110</startdate><enddate>202110</enddate><creator>Sood, Richa</creator><creator>Kumar, Naveen</creator><creator>Gokhe, Suresh S.</creator><creator>Pateriya, Atul Kumar</creator><creator>Bhat, Sushant</creator><creator>Bhatia, Sandeep</creator><creator>Panickan, Sivasankar</creator><creator>Mishra, Anamika</creator><creator>Murugkar, H.V.</creator><creator>Dixit, Roma</creator><creator>Shrivastava, Deepali</creator><creator>Singh, Pushpendra</creator><creator>Tripathi, Meghna</creator><creator>Singh, Vijendra Pal</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202110</creationdate><title>Identification and molecular characterization of H9N2 viruses carrying multiple mammalian adaptation markers in resident birds in central-western wetlands in India</title><author>Sood, Richa ; Kumar, Naveen ; Gokhe, Suresh S. ; Pateriya, Atul Kumar ; Bhat, Sushant ; Bhatia, Sandeep ; Panickan, Sivasankar ; Mishra, Anamika ; Murugkar, H.V. ; Dixit, Roma ; Shrivastava, Deepali ; Singh, Pushpendra ; Tripathi, Meghna ; Singh, Vijendra Pal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-d1365f439b3b6fe4d856052be64233aeef5cbc5ff6c2cf653704b1b2b08465c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adaptation, Biological</topic><topic>Animals</topic><topic>Biosecurity</topic><topic>Birds</topic><topic>H9N2 viruses</topic><topic>India</topic><topic>India - epidemiology</topic><topic>Influenza A Virus, H9N2 Subtype - genetics</topic><topic>Influenza in Birds - epidemiology</topic><topic>Influenza in Birds - virology</topic><topic>Mammals</topic><topic>Prevalence</topic><topic>Resident birds</topic><topic>Wetlands</topic><topic>Zoonotic potential</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sood, Richa</creatorcontrib><creatorcontrib>Kumar, Naveen</creatorcontrib><creatorcontrib>Gokhe, Suresh S.</creatorcontrib><creatorcontrib>Pateriya, Atul Kumar</creatorcontrib><creatorcontrib>Bhat, Sushant</creatorcontrib><creatorcontrib>Bhatia, Sandeep</creatorcontrib><creatorcontrib>Panickan, Sivasankar</creatorcontrib><creatorcontrib>Mishra, Anamika</creatorcontrib><creatorcontrib>Murugkar, H.V.</creatorcontrib><creatorcontrib>Dixit, Roma</creatorcontrib><creatorcontrib>Shrivastava, Deepali</creatorcontrib><creatorcontrib>Singh, Pushpendra</creatorcontrib><creatorcontrib>Tripathi, Meghna</creatorcontrib><creatorcontrib>Singh, Vijendra Pal</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Infection, genetics and evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sood, Richa</au><au>Kumar, Naveen</au><au>Gokhe, Suresh S.</au><au>Pateriya, Atul Kumar</au><au>Bhat, Sushant</au><au>Bhatia, Sandeep</au><au>Panickan, Sivasankar</au><au>Mishra, Anamika</au><au>Murugkar, H.V.</au><au>Dixit, Roma</au><au>Shrivastava, Deepali</au><au>Singh, Pushpendra</au><au>Tripathi, Meghna</au><au>Singh, Vijendra Pal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and molecular characterization of H9N2 viruses carrying multiple mammalian adaptation markers in resident birds in central-western wetlands in India</atitle><jtitle>Infection, genetics and evolution</jtitle><addtitle>Infect Genet Evol</addtitle><date>2021-10</date><risdate>2021</risdate><volume>94</volume><spage>105005</spage><epage>105005</epage><pages>105005-105005</pages><artnum>105005</artnum><issn>1567-1348</issn><eissn>1567-7257</eissn><abstract>We report here a targeted risk-based study to investigate the presence of influenza A viruses at the migratory-wild-domestic bird interface across the major wetlands of central India's Maharashtra state during the winter migration season. The H9N2 viruses have been isolated and confirmed in 3.86% (33/854) of the fecal samples of resident birds. To investigate the genetic pools of H9N2 circulating in resident birds, we sequenced two isolates of H9N2 from distant wetlands. Sequence and phylogenetic analyses have shown that these viruses are triple reassortants, with HA, NA, NP, and M genes belonging to G1 sub-lineage (A/quail/Hong Kong/G1/1997), PB2, PB1, and NS genes originating from the prototype Eurasian lineage (A/mallard/France/090360/2009) and PA gene deriving from Y439/Korean-like (A/duck/Hong Kong/Y439/97) sub-lineage. It was confirmed not only that four of their gene segments had a high genetic association with the zoonotic H9N2 virus, A/Human/India/TCM2581/2019, but also that they had many molecular markers associated with mammalian adaptation and enhanced virulence in mammals including the unique multiple basic amino acids, KSKR↓GLF at the HA cleavage site, and analog N-and O-glycosylation patterns on HA with that of the zoonotic H9N2 virus. Furthermore, future experiments would be to characterize these isolates biologically to address the public health concern. Importantly, due to the identification of these viruses at a strategic geographical location in India (a major stop-over point in the Central Asian flyway), these novel viruses also pose a possible threat to be exported to other regions via migratory/resident birds. Consequently, systematic investigation and active monitoring are a prerequisite for identifying and preventing the spread of viruses of zoonotic potential by enforcing strict biosecurity measures.
•First influenza H9N2 virus study at migratory-wild-domestic bird interface from India.•Triple reassortants H9N2 viruses were isolated from major wetlands of central India.•H9N2 viruses contained many markers for virulence and adaptation in mammalian hosts.•Strong negative purifying selection governs evolution of the Indian H9N2 viruses.•Glycosylation patterns on hemagglutinin match with that of the zoonotic H9N2 virus.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>34293481</pmid><doi>10.1016/j.meegid.2021.105005</doi><tpages>1</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1567-1348 |
ispartof | Infection, genetics and evolution, 2021-10, Vol.94, p.105005-105005, Article 105005 |
issn | 1567-1348 1567-7257 |
language | eng |
recordid | cdi_proquest_miscellaneous_2555113664 |
source | MEDLINE; Access via ScienceDirect (Elsevier) |
subjects | Adaptation, Biological Animals Biosecurity Birds H9N2 viruses India India - epidemiology Influenza A Virus, H9N2 Subtype - genetics Influenza in Birds - epidemiology Influenza in Birds - virology Mammals Prevalence Resident birds Wetlands Zoonotic potential |
title | Identification and molecular characterization of H9N2 viruses carrying multiple mammalian adaptation markers in resident birds in central-western wetlands in India |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T23%3A59%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20and%20molecular%20characterization%20of%20H9N2%20viruses%20carrying%20multiple%20mammalian%20adaptation%20markers%20in%20resident%20birds%20in%20central-western%20wetlands%20in%20India&rft.jtitle=Infection,%20genetics%20and%20evolution&rft.au=Sood,%20Richa&rft.date=2021-10&rft.volume=94&rft.spage=105005&rft.epage=105005&rft.pages=105005-105005&rft.artnum=105005&rft.issn=1567-1348&rft.eissn=1567-7257&rft_id=info:doi/10.1016/j.meegid.2021.105005&rft_dat=%3Cproquest_cross%3E2555113664%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2555113664&rft_id=info:pmid/34293481&rft_els_id=S1567134821003038&rfr_iscdi=true |