Identification of the fate and regenerative mechanism of zebrafish melanocyte progenitor cells and melanocytes after laser‐induced pigment ablation
Background and Objectives Lasers are known to be the most effective treatment modality for pigmentary skin diseases. However, melanocytes and melanin pigment often recur or leave post‐inflammatory hyperpigmentation after the laser procedure. Studies have reported on the role of progenitor cells in p...
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| Veröffentlicht in: | Lasers in surgery and medicine 2022-02, Vol.54 (2), p.281-288 |
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| creator | Park, Ji Hyun Koun, Soonil Kim, Ko Eun Kim, Il‐Hwan |
| description | Background and Objectives
Lasers are known to be the most effective treatment modality for pigmentary skin diseases. However, melanocytes and melanin pigment often recur or leave post‐inflammatory hyperpigmentation after the laser procedure. Studies have reported on the role of progenitor cells in pigment cell regeneration, which can be constantly replenished through mitosis. However, the response of unpigmented melanocyte progenitor cells to laser treatment is poorly understood. In this study, we used adult zebrafish skin as the melanocyte regenerative system and examined the response of melanocyte progenitor cells to laser photothermolysis.
Materials and Methods
The two groups of adult zebrafish were irradiated with 1064 nm wavelength laser system of Q‐switched neodymium:yttrium–aluminum–garnet (Nd:YAG) laser with 0.3 or 0.7 J·cm−2. We compared the regeneration of pigment at different energy levels by measuring new melanocyte counts and pigment area. We traced and quantitatively compared the melanocyte lineage cells by immunohistochemical staining using specific markers such as sox10, mitfa, and dct during the regeneration process. Three repetitive laser ablations were also held to test the postinflammatory hyperpigmentation.
Results
After the laser ablation of melanocytes, most of the new melanocytes appeared between Days 5 and 10. In high‐energy irradiation of 0.7 J·cm−2, the unpigmented mitfa‐expressing cells showed significant decrease (p |
| doi_str_mv | 10.1002/lsm.23458 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2555104518</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2630955885</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3538-4ed890cc1dbd402ec6ee8ffaace7fd5eeee997a6ca0c05393ec80e2dfde8094e3</originalsourceid><addsrcrecordid>eNp1kcFuEzEQhi0EoqFw4AWQJS5wSDu214l9rCpKKwVxaDlbjj1uXO16g70LSk99hF54QZ4EJ2lBqsRcPPZ8_vVrfkLeMjhiAPy4Ld0RF41Uz8iEgZ5NNQP2nEyA1V6B5gfkVSk3ACA4zF-SA9FwraRSE_LrwmMaYojODrFPtA90WCENdkBqk6cZrzFhrsMfSDt0K5ti6bbYLS6zDbGs6nNrU-829cs695WPQ5-pw7YtO41_83oPA2ba2oL59919TH506Ok6XnfVBrXLdmfjNXkRbFvwzcN5SL6dfbo6PZ8uvn6-OD1ZTJ2QQk0b9EqDc8wvfQMc3QxRhWCtw3nwEmtpPbczZ8GBFFqgU4DcB491Kw2KQ_Jhr1t9fx-xDKaLZWvcJuzHYriUkkEjmaro-yfoTT_mVN0ZPhOgZV2nrNTHPeVyX0rGYNY5djZvDAOzzcrUrMwuq8q-e1Aclx36v-RjOBU43gM_Y4ub_yuZxeWXveQfF-Sj4w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2630955885</pqid></control><display><type>article</type><title>Identification of the fate and regenerative mechanism of zebrafish melanocyte progenitor cells and melanocytes after laser‐induced pigment ablation</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Park, Ji Hyun ; Koun, Soonil ; Kim, Ko Eun ; Kim, Il‐Hwan</creator><creatorcontrib>Park, Ji Hyun ; Koun, Soonil ; Kim, Ko Eun ; Kim, Il‐Hwan</creatorcontrib><description>Background and Objectives
Lasers are known to be the most effective treatment modality for pigmentary skin diseases. However, melanocytes and melanin pigment often recur or leave post‐inflammatory hyperpigmentation after the laser procedure. Studies have reported on the role of progenitor cells in pigment cell regeneration, which can be constantly replenished through mitosis. However, the response of unpigmented melanocyte progenitor cells to laser treatment is poorly understood. In this study, we used adult zebrafish skin as the melanocyte regenerative system and examined the response of melanocyte progenitor cells to laser photothermolysis.
Materials and Methods
The two groups of adult zebrafish were irradiated with 1064 nm wavelength laser system of Q‐switched neodymium:yttrium–aluminum–garnet (Nd:YAG) laser with 0.3 or 0.7 J·cm−2. We compared the regeneration of pigment at different energy levels by measuring new melanocyte counts and pigment area. We traced and quantitatively compared the melanocyte lineage cells by immunohistochemical staining using specific markers such as sox10, mitfa, and dct during the regeneration process. Three repetitive laser ablations were also held to test the postinflammatory hyperpigmentation.
Results
After the laser ablation of melanocytes, most of the new melanocytes appeared between Days 5 and 10. In high‐energy irradiation of 0.7 J·cm−2, the unpigmented mitfa‐expressing cells showed significant decrease (p < 0.05) and showed delay in the differentiation process of melanocyte lineage cells. After repeated laser irradiation, hyperpigmentation did not appear and the final recovery ratio of the pigmented area was 87.5% and 75.3% at the 0.3 and 0.7 J·cm−2 energy levels, respectively.
Conclusion
We suggest that laser treatment overcoming the recurrence should be planned based on the adequate energy level targeting the melanocyte progenitor cells. High‐energy irradiation may induce apoptosis of progenitor cells and delay their process of differentiation. Short‐term repetitive sessions of laser therapy can reduce the pigmentation in the long‐term observation.</description><identifier>ISSN: 0196-8092</identifier><identifier>EISSN: 1096-9101</identifier><identifier>DOI: 10.1002/lsm.23458</identifier><identifier>PMID: 34298588</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Ablation ; Aluminum ; Animals ; Apoptosis ; Cell differentiation ; Cells (biology) ; Danio rerio ; Differentiation ; Energy ; Hyperpigmentation ; Hyperpigmentation - etiology ; Hyperpigmentation - surgery ; Inflammation ; Irradiation ; laser ; Laser ablation ; Lasers ; Lasers, Solid-State - therapeutic use ; Low-Level Light Therapy ; Melanin ; melanocyte ; Melanocytes ; Mitosis ; Neodymium ; Neodymium lasers ; Pigmentation ; postinflammatory hyperpigmentation ; progenitor cell ; Progenitor cells ; Regeneration ; Skin diseases ; Sox10 protein ; Stem Cells ; YAG lasers ; Yttrium ; Zebrafish</subject><ispartof>Lasers in surgery and medicine, 2022-02, Vol.54 (2), p.281-288</ispartof><rights>2021 Wiley Periodicals LLC</rights><rights>2021 Wiley Periodicals LLC.</rights><rights>2022 Wiley Periodicals LLC</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3538-4ed890cc1dbd402ec6ee8ffaace7fd5eeee997a6ca0c05393ec80e2dfde8094e3</citedby><cites>FETCH-LOGICAL-c3538-4ed890cc1dbd402ec6ee8ffaace7fd5eeee997a6ca0c05393ec80e2dfde8094e3</cites><orcidid>0000-0002-4141-204X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Flsm.23458$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Flsm.23458$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34298588$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Ji Hyun</creatorcontrib><creatorcontrib>Koun, Soonil</creatorcontrib><creatorcontrib>Kim, Ko Eun</creatorcontrib><creatorcontrib>Kim, Il‐Hwan</creatorcontrib><title>Identification of the fate and regenerative mechanism of zebrafish melanocyte progenitor cells and melanocytes after laser‐induced pigment ablation</title><title>Lasers in surgery and medicine</title><addtitle>Lasers Surg Med</addtitle><description>Background and Objectives
Lasers are known to be the most effective treatment modality for pigmentary skin diseases. However, melanocytes and melanin pigment often recur or leave post‐inflammatory hyperpigmentation after the laser procedure. Studies have reported on the role of progenitor cells in pigment cell regeneration, which can be constantly replenished through mitosis. However, the response of unpigmented melanocyte progenitor cells to laser treatment is poorly understood. In this study, we used adult zebrafish skin as the melanocyte regenerative system and examined the response of melanocyte progenitor cells to laser photothermolysis.
Materials and Methods
The two groups of adult zebrafish were irradiated with 1064 nm wavelength laser system of Q‐switched neodymium:yttrium–aluminum–garnet (Nd:YAG) laser with 0.3 or 0.7 J·cm−2. We compared the regeneration of pigment at different energy levels by measuring new melanocyte counts and pigment area. We traced and quantitatively compared the melanocyte lineage cells by immunohistochemical staining using specific markers such as sox10, mitfa, and dct during the regeneration process. Three repetitive laser ablations were also held to test the postinflammatory hyperpigmentation.
Results
After the laser ablation of melanocytes, most of the new melanocytes appeared between Days 5 and 10. In high‐energy irradiation of 0.7 J·cm−2, the unpigmented mitfa‐expressing cells showed significant decrease (p < 0.05) and showed delay in the differentiation process of melanocyte lineage cells. After repeated laser irradiation, hyperpigmentation did not appear and the final recovery ratio of the pigmented area was 87.5% and 75.3% at the 0.3 and 0.7 J·cm−2 energy levels, respectively.
Conclusion
We suggest that laser treatment overcoming the recurrence should be planned based on the adequate energy level targeting the melanocyte progenitor cells. High‐energy irradiation may induce apoptosis of progenitor cells and delay their process of differentiation. Short‐term repetitive sessions of laser therapy can reduce the pigmentation in the long‐term observation.</description><subject>Ablation</subject><subject>Aluminum</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Cell differentiation</subject><subject>Cells (biology)</subject><subject>Danio rerio</subject><subject>Differentiation</subject><subject>Energy</subject><subject>Hyperpigmentation</subject><subject>Hyperpigmentation - etiology</subject><subject>Hyperpigmentation - surgery</subject><subject>Inflammation</subject><subject>Irradiation</subject><subject>laser</subject><subject>Laser ablation</subject><subject>Lasers</subject><subject>Lasers, Solid-State - therapeutic use</subject><subject>Low-Level Light Therapy</subject><subject>Melanin</subject><subject>melanocyte</subject><subject>Melanocytes</subject><subject>Mitosis</subject><subject>Neodymium</subject><subject>Neodymium lasers</subject><subject>Pigmentation</subject><subject>postinflammatory hyperpigmentation</subject><subject>progenitor cell</subject><subject>Progenitor cells</subject><subject>Regeneration</subject><subject>Skin diseases</subject><subject>Sox10 protein</subject><subject>Stem Cells</subject><subject>YAG lasers</subject><subject>Yttrium</subject><subject>Zebrafish</subject><issn>0196-8092</issn><issn>1096-9101</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFuEzEQhi0EoqFw4AWQJS5wSDu214l9rCpKKwVxaDlbjj1uXO16g70LSk99hF54QZ4EJ2lBqsRcPPZ8_vVrfkLeMjhiAPy4Ld0RF41Uz8iEgZ5NNQP2nEyA1V6B5gfkVSk3ACA4zF-SA9FwraRSE_LrwmMaYojODrFPtA90WCENdkBqk6cZrzFhrsMfSDt0K5ti6bbYLS6zDbGs6nNrU-829cs695WPQ5-pw7YtO41_83oPA2ba2oL59919TH506Ok6XnfVBrXLdmfjNXkRbFvwzcN5SL6dfbo6PZ8uvn6-OD1ZTJ2QQk0b9EqDc8wvfQMc3QxRhWCtw3nwEmtpPbczZ8GBFFqgU4DcB491Kw2KQ_Jhr1t9fx-xDKaLZWvcJuzHYriUkkEjmaro-yfoTT_mVN0ZPhOgZV2nrNTHPeVyX0rGYNY5djZvDAOzzcrUrMwuq8q-e1Aclx36v-RjOBU43gM_Y4ub_yuZxeWXveQfF-Sj4w</recordid><startdate>202202</startdate><enddate>202202</enddate><creator>Park, Ji Hyun</creator><creator>Koun, Soonil</creator><creator>Kim, Ko Eun</creator><creator>Kim, Il‐Hwan</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4141-204X</orcidid></search><sort><creationdate>202202</creationdate><title>Identification of the fate and regenerative mechanism of zebrafish melanocyte progenitor cells and melanocytes after laser‐induced pigment ablation</title><author>Park, Ji Hyun ; Koun, Soonil ; Kim, Ko Eun ; Kim, Il‐Hwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3538-4ed890cc1dbd402ec6ee8ffaace7fd5eeee997a6ca0c05393ec80e2dfde8094e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Ablation</topic><topic>Aluminum</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Cell differentiation</topic><topic>Cells (biology)</topic><topic>Danio rerio</topic><topic>Differentiation</topic><topic>Energy</topic><topic>Hyperpigmentation</topic><topic>Hyperpigmentation - etiology</topic><topic>Hyperpigmentation - surgery</topic><topic>Inflammation</topic><topic>Irradiation</topic><topic>laser</topic><topic>Laser ablation</topic><topic>Lasers</topic><topic>Lasers, Solid-State - therapeutic use</topic><topic>Low-Level Light Therapy</topic><topic>Melanin</topic><topic>melanocyte</topic><topic>Melanocytes</topic><topic>Mitosis</topic><topic>Neodymium</topic><topic>Neodymium lasers</topic><topic>Pigmentation</topic><topic>postinflammatory hyperpigmentation</topic><topic>progenitor cell</topic><topic>Progenitor cells</topic><topic>Regeneration</topic><topic>Skin diseases</topic><topic>Sox10 protein</topic><topic>Stem Cells</topic><topic>YAG lasers</topic><topic>Yttrium</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Ji Hyun</creatorcontrib><creatorcontrib>Koun, Soonil</creatorcontrib><creatorcontrib>Kim, Ko Eun</creatorcontrib><creatorcontrib>Kim, Il‐Hwan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Lasers in surgery and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Ji Hyun</au><au>Koun, Soonil</au><au>Kim, Ko Eun</au><au>Kim, Il‐Hwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of the fate and regenerative mechanism of zebrafish melanocyte progenitor cells and melanocytes after laser‐induced pigment ablation</atitle><jtitle>Lasers in surgery and medicine</jtitle><addtitle>Lasers Surg Med</addtitle><date>2022-02</date><risdate>2022</risdate><volume>54</volume><issue>2</issue><spage>281</spage><epage>288</epage><pages>281-288</pages><issn>0196-8092</issn><eissn>1096-9101</eissn><abstract>Background and Objectives
Lasers are known to be the most effective treatment modality for pigmentary skin diseases. However, melanocytes and melanin pigment often recur or leave post‐inflammatory hyperpigmentation after the laser procedure. Studies have reported on the role of progenitor cells in pigment cell regeneration, which can be constantly replenished through mitosis. However, the response of unpigmented melanocyte progenitor cells to laser treatment is poorly understood. In this study, we used adult zebrafish skin as the melanocyte regenerative system and examined the response of melanocyte progenitor cells to laser photothermolysis.
Materials and Methods
The two groups of adult zebrafish were irradiated with 1064 nm wavelength laser system of Q‐switched neodymium:yttrium–aluminum–garnet (Nd:YAG) laser with 0.3 or 0.7 J·cm−2. We compared the regeneration of pigment at different energy levels by measuring new melanocyte counts and pigment area. We traced and quantitatively compared the melanocyte lineage cells by immunohistochemical staining using specific markers such as sox10, mitfa, and dct during the regeneration process. Three repetitive laser ablations were also held to test the postinflammatory hyperpigmentation.
Results
After the laser ablation of melanocytes, most of the new melanocytes appeared between Days 5 and 10. In high‐energy irradiation of 0.7 J·cm−2, the unpigmented mitfa‐expressing cells showed significant decrease (p < 0.05) and showed delay in the differentiation process of melanocyte lineage cells. After repeated laser irradiation, hyperpigmentation did not appear and the final recovery ratio of the pigmented area was 87.5% and 75.3% at the 0.3 and 0.7 J·cm−2 energy levels, respectively.
Conclusion
We suggest that laser treatment overcoming the recurrence should be planned based on the adequate energy level targeting the melanocyte progenitor cells. High‐energy irradiation may induce apoptosis of progenitor cells and delay their process of differentiation. Short‐term repetitive sessions of laser therapy can reduce the pigmentation in the long‐term observation.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>34298588</pmid><doi>10.1002/lsm.23458</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-4141-204X</orcidid></addata></record> |
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| ispartof | Lasers in surgery and medicine, 2022-02, Vol.54 (2), p.281-288 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Ablation Aluminum Animals Apoptosis Cell differentiation Cells (biology) Danio rerio Differentiation Energy Hyperpigmentation Hyperpigmentation - etiology Hyperpigmentation - surgery Inflammation Irradiation laser Laser ablation Lasers Lasers, Solid-State - therapeutic use Low-Level Light Therapy Melanin melanocyte Melanocytes Mitosis Neodymium Neodymium lasers Pigmentation postinflammatory hyperpigmentation progenitor cell Progenitor cells Regeneration Skin diseases Sox10 protein Stem Cells YAG lasers Yttrium Zebrafish |
| title | Identification of the fate and regenerative mechanism of zebrafish melanocyte progenitor cells and melanocytes after laser‐induced pigment ablation |
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