Glutamate Chemical Exchange Saturation Transfer Imaging and Functional Alterations of Hippocampus in Rat Depression Model: A Pilot Study
Background Adjusting abnormal glutamate neurotransmission is a crucial mechanism in the treatment of depression. However, few non‐invasive techniques could effectively detect changes in glutamate neurotransmitters, and no consensus exists on whether glutamate could affect resting‐state function chan...
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| Veröffentlicht in: | Journal of magnetic resonance imaging 2021-12, Vol.54 (6), p.1967-1976 |
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| container_end_page | 1976 |
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| container_issue | 6 |
| container_start_page | 1967 |
| container_title | Journal of magnetic resonance imaging |
| container_volume | 54 |
| creator | Luo, Xunrong Ren, Qingfa Luo, Mingfang Li, Tianping Lv, Yijie Liu, Yan Rong, Kang Zhang, Wei Li, Xianglin |
| description | Background
Adjusting abnormal glutamate neurotransmission is a crucial mechanism in the treatment of depression. However, few non‐invasive techniques could effectively detect changes in glutamate neurotransmitters, and no consensus exists on whether glutamate could affect resting‐state function changes in depression.
Purpose
To study the changes in glutamate chemical exchange saturation transfer (GluCEST) value in the hippocampus of rat model exposed to chronic unpredictable mild stress (CUMS), and to explore the effect of this change on the activity of hippocampal glutamatergic neurons.
Study Type
Prospective animal study.
Animal Model
Twenty male Sprague–Dawley rats (200–300 g).
Field Strength/Sequence
7.0 T scanner. Fat rapid acquisition relaxation enhancement sequence for GluCEST, and echo planner imaging sequence for resting‐state functional magnetic resonance imaging (rs_fMRI).
Assessment
Rats were divided into two groups: CUMS group (N = 10) and control group (CTRL, N = 10). The magnetization transfer ratio asymmetry analysis was used to quantify the GluCEST data, and evaluate the rs_fMRI data through the amplitude of low‐frequency fluctuation (ALFF) and regional homogeneity (ReHo) analysis.
Statistical Tests
A t‐test was used to compare the difference in GluCEST or rs_fMRI between CUMS and CTRL groups. Spearman's correlation was applied to explore the correlation between GluCEST values and abnormal fMRI values in hippocampus. Statistical significance was set at P |
| doi_str_mv | 10.1002/jmri.27850 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2554350847</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2554350847</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3570-166b7b2c841c69578ebb3cd15554b036147e0cae8196988a061e0c436bd819b3</originalsourceid><addsrcrecordid>eNp9kctu1DAUhi1ERUthwwMgS2wQUoodX2KzG01vg1qB2tlHjuOZeuTEwReVeQMeGw9pWbBg5eNzvvNJRz8A7zA6wwjVn3dDsGd1Ixh6AU4wq-uqZoK_LDVipMICNcfgdYw7hJCUlL0Cx4TWEgtGT8CvK5eTGlQycPlgBquVgxc_9YMatwbeq5SDStaPcB3UGDcmwNWgtnbcQjX28DKP-jAtOwuXzIxG6Dfw2k6T12qYcoR2hHcqwXMzBRPjQXbre-O-wAX8bp1P8D7lfv8GHG2Ui-bt03sK1pcX6-V1dfPtarVc3FSasAZVmPOu6WotKNZcskaYriO6x4wx2iHCMW0M0soILLkUQiGOy58S3vWl1ZFT8HHWTsH_yCamdrBRG-fUaHyObV08hCFBm4J--Afd-RzKsYXiiEjJkKSF-jRTOvgYg9m0U7CDCvsWo_YQT3uIp_0TT4HfPylzN5j-L_qcRwHwDDxaZ_b_UbVfb-9Ws_Q3X-Walw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2603995094</pqid></control><display><type>article</type><title>Glutamate Chemical Exchange Saturation Transfer Imaging and Functional Alterations of Hippocampus in Rat Depression Model: A Pilot Study</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Luo, Xunrong ; Ren, Qingfa ; Luo, Mingfang ; Li, Tianping ; Lv, Yijie ; Liu, Yan ; Rong, Kang ; Zhang, Wei ; Li, Xianglin</creator><creatorcontrib>Luo, Xunrong ; Ren, Qingfa ; Luo, Mingfang ; Li, Tianping ; Lv, Yijie ; Liu, Yan ; Rong, Kang ; Zhang, Wei ; Li, Xianglin</creatorcontrib><description>Background
Adjusting abnormal glutamate neurotransmission is a crucial mechanism in the treatment of depression. However, few non‐invasive techniques could effectively detect changes in glutamate neurotransmitters, and no consensus exists on whether glutamate could affect resting‐state function changes in depression.
Purpose
To study the changes in glutamate chemical exchange saturation transfer (GluCEST) value in the hippocampus of rat model exposed to chronic unpredictable mild stress (CUMS), and to explore the effect of this change on the activity of hippocampal glutamatergic neurons.
Study Type
Prospective animal study.
Animal Model
Twenty male Sprague–Dawley rats (200–300 g).
Field Strength/Sequence
7.0 T scanner. Fat rapid acquisition relaxation enhancement sequence for GluCEST, and echo planner imaging sequence for resting‐state functional magnetic resonance imaging (rs_fMRI).
Assessment
Rats were divided into two groups: CUMS group (N = 10) and control group (CTRL, N = 10). The magnetization transfer ratio asymmetry analysis was used to quantify the GluCEST data, and evaluate the rs_fMRI data through the amplitude of low‐frequency fluctuation (ALFF) and regional homogeneity (ReHo) analysis.
Statistical Tests
A t‐test was used to compare the difference in GluCEST or rs_fMRI between CUMS and CTRL groups. Spearman's correlation was applied to explore the correlation between GluCEST values and abnormal fMRI values in hippocampus. Statistical significance was set at P < 0.05.
Results
The GluCEST value in the left hippocampus has changed significantly (3.3 ± 0.3 [CUMS] vs. 3.9 ± 0.4 [CTRL], P < 0.05). In addition, the GluCEST value was significantly positively correlated with the ALFF values (r = 0.5, P < 0. 05, df = 7) and negatively correlated with the ReHo values (r = −0.6, P < 0.05, df = 7).
Data Conclusion
GluCEST technique has the feasibility of mapping glutamate changes in rat depression. Glutamate neurotransmitters are important factors affecting the abnormal function of neural activity.
Level of Evidence
2
Technical Efficacy
Stage 1</description><identifier>ISSN: 1053-1807</identifier><identifier>EISSN: 1522-2586</identifier><identifier>DOI: 10.1002/jmri.27850</identifier><identifier>PMID: 34291854</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Animal models ; Animals ; Brain ; Brain Mapping ; Change detection ; chemical exchange saturation transfer ; Correlation ; depression ; Depression - diagnostic imaging ; Field strength ; Functional magnetic resonance imaging ; glutamate ; Glutamatergic transmission ; Glutamic Acid ; Hippocampus ; Hippocampus - diagnostic imaging ; Homogeneity ; Magnetic Resonance Imaging ; Male ; Neurotransmission ; Neurotransmitters ; Pilot Projects ; Prospective Studies ; Rats ; Rats, Sprague-Dawley ; resting‐state functional magnetic resonance imaging ; Saturation ; Statistical analysis ; Statistical tests</subject><ispartof>Journal of magnetic resonance imaging, 2021-12, Vol.54 (6), p.1967-1976</ispartof><rights>2021 International Society for Magnetic Resonance in Medicine.</rights><rights>2021 International Society for Magnetic Resonance in Medicine</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3570-166b7b2c841c69578ebb3cd15554b036147e0cae8196988a061e0c436bd819b3</citedby><cites>FETCH-LOGICAL-c3570-166b7b2c841c69578ebb3cd15554b036147e0cae8196988a061e0c436bd819b3</cites><orcidid>0000-0003-4469-3390 ; 0000-0002-1531-2520</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmri.27850$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmri.27850$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34291854$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luo, Xunrong</creatorcontrib><creatorcontrib>Ren, Qingfa</creatorcontrib><creatorcontrib>Luo, Mingfang</creatorcontrib><creatorcontrib>Li, Tianping</creatorcontrib><creatorcontrib>Lv, Yijie</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Rong, Kang</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Li, Xianglin</creatorcontrib><title>Glutamate Chemical Exchange Saturation Transfer Imaging and Functional Alterations of Hippocampus in Rat Depression Model: A Pilot Study</title><title>Journal of magnetic resonance imaging</title><addtitle>J Magn Reson Imaging</addtitle><description>Background
Adjusting abnormal glutamate neurotransmission is a crucial mechanism in the treatment of depression. However, few non‐invasive techniques could effectively detect changes in glutamate neurotransmitters, and no consensus exists on whether glutamate could affect resting‐state function changes in depression.
Purpose
To study the changes in glutamate chemical exchange saturation transfer (GluCEST) value in the hippocampus of rat model exposed to chronic unpredictable mild stress (CUMS), and to explore the effect of this change on the activity of hippocampal glutamatergic neurons.
Study Type
Prospective animal study.
Animal Model
Twenty male Sprague–Dawley rats (200–300 g).
Field Strength/Sequence
7.0 T scanner. Fat rapid acquisition relaxation enhancement sequence for GluCEST, and echo planner imaging sequence for resting‐state functional magnetic resonance imaging (rs_fMRI).
Assessment
Rats were divided into two groups: CUMS group (N = 10) and control group (CTRL, N = 10). The magnetization transfer ratio asymmetry analysis was used to quantify the GluCEST data, and evaluate the rs_fMRI data through the amplitude of low‐frequency fluctuation (ALFF) and regional homogeneity (ReHo) analysis.
Statistical Tests
A t‐test was used to compare the difference in GluCEST or rs_fMRI between CUMS and CTRL groups. Spearman's correlation was applied to explore the correlation between GluCEST values and abnormal fMRI values in hippocampus. Statistical significance was set at P < 0.05.
Results
The GluCEST value in the left hippocampus has changed significantly (3.3 ± 0.3 [CUMS] vs. 3.9 ± 0.4 [CTRL], P < 0.05). In addition, the GluCEST value was significantly positively correlated with the ALFF values (r = 0.5, P < 0. 05, df = 7) and negatively correlated with the ReHo values (r = −0.6, P < 0.05, df = 7).
Data Conclusion
GluCEST technique has the feasibility of mapping glutamate changes in rat depression. Glutamate neurotransmitters are important factors affecting the abnormal function of neural activity.
Level of Evidence
2
Technical Efficacy
Stage 1</description><subject>Animal models</subject><subject>Animals</subject><subject>Brain</subject><subject>Brain Mapping</subject><subject>Change detection</subject><subject>chemical exchange saturation transfer</subject><subject>Correlation</subject><subject>depression</subject><subject>Depression - diagnostic imaging</subject><subject>Field strength</subject><subject>Functional magnetic resonance imaging</subject><subject>glutamate</subject><subject>Glutamatergic transmission</subject><subject>Glutamic Acid</subject><subject>Hippocampus</subject><subject>Hippocampus - diagnostic imaging</subject><subject>Homogeneity</subject><subject>Magnetic Resonance Imaging</subject><subject>Male</subject><subject>Neurotransmission</subject><subject>Neurotransmitters</subject><subject>Pilot Projects</subject><subject>Prospective Studies</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>resting‐state functional magnetic resonance imaging</subject><subject>Saturation</subject><subject>Statistical analysis</subject><subject>Statistical tests</subject><issn>1053-1807</issn><issn>1522-2586</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1DAUhi1ERUthwwMgS2wQUoodX2KzG01vg1qB2tlHjuOZeuTEwReVeQMeGw9pWbBg5eNzvvNJRz8A7zA6wwjVn3dDsGd1Ixh6AU4wq-uqZoK_LDVipMICNcfgdYw7hJCUlL0Cx4TWEgtGT8CvK5eTGlQycPlgBquVgxc_9YMatwbeq5SDStaPcB3UGDcmwNWgtnbcQjX28DKP-jAtOwuXzIxG6Dfw2k6T12qYcoR2hHcqwXMzBRPjQXbre-O-wAX8bp1P8D7lfv8GHG2Ui-bt03sK1pcX6-V1dfPtarVc3FSasAZVmPOu6WotKNZcskaYriO6x4wx2iHCMW0M0soILLkUQiGOy58S3vWl1ZFT8HHWTsH_yCamdrBRG-fUaHyObV08hCFBm4J--Afd-RzKsYXiiEjJkKSF-jRTOvgYg9m0U7CDCvsWo_YQT3uIp_0TT4HfPylzN5j-L_qcRwHwDDxaZ_b_UbVfb-9Ws_Q3X-Walw</recordid><startdate>202112</startdate><enddate>202112</enddate><creator>Luo, Xunrong</creator><creator>Ren, Qingfa</creator><creator>Luo, Mingfang</creator><creator>Li, Tianping</creator><creator>Lv, Yijie</creator><creator>Liu, Yan</creator><creator>Rong, Kang</creator><creator>Zhang, Wei</creator><creator>Li, Xianglin</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4469-3390</orcidid><orcidid>https://orcid.org/0000-0002-1531-2520</orcidid></search><sort><creationdate>202112</creationdate><title>Glutamate Chemical Exchange Saturation Transfer Imaging and Functional Alterations of Hippocampus in Rat Depression Model: A Pilot Study</title><author>Luo, Xunrong ; Ren, Qingfa ; Luo, Mingfang ; Li, Tianping ; Lv, Yijie ; Liu, Yan ; Rong, Kang ; Zhang, Wei ; Li, Xianglin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3570-166b7b2c841c69578ebb3cd15554b036147e0cae8196988a061e0c436bd819b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animal models</topic><topic>Animals</topic><topic>Brain</topic><topic>Brain Mapping</topic><topic>Change detection</topic><topic>chemical exchange saturation transfer</topic><topic>Correlation</topic><topic>depression</topic><topic>Depression - diagnostic imaging</topic><topic>Field strength</topic><topic>Functional magnetic resonance imaging</topic><topic>glutamate</topic><topic>Glutamatergic transmission</topic><topic>Glutamic Acid</topic><topic>Hippocampus</topic><topic>Hippocampus - diagnostic imaging</topic><topic>Homogeneity</topic><topic>Magnetic Resonance Imaging</topic><topic>Male</topic><topic>Neurotransmission</topic><topic>Neurotransmitters</topic><topic>Pilot Projects</topic><topic>Prospective Studies</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>resting‐state functional magnetic resonance imaging</topic><topic>Saturation</topic><topic>Statistical analysis</topic><topic>Statistical tests</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Xunrong</creatorcontrib><creatorcontrib>Ren, Qingfa</creatorcontrib><creatorcontrib>Luo, Mingfang</creatorcontrib><creatorcontrib>Li, Tianping</creatorcontrib><creatorcontrib>Lv, Yijie</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Rong, Kang</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Li, Xianglin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of magnetic resonance imaging</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Xunrong</au><au>Ren, Qingfa</au><au>Luo, Mingfang</au><au>Li, Tianping</au><au>Lv, Yijie</au><au>Liu, Yan</au><au>Rong, Kang</au><au>Zhang, Wei</au><au>Li, Xianglin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glutamate Chemical Exchange Saturation Transfer Imaging and Functional Alterations of Hippocampus in Rat Depression Model: A Pilot Study</atitle><jtitle>Journal of magnetic resonance imaging</jtitle><addtitle>J Magn Reson Imaging</addtitle><date>2021-12</date><risdate>2021</risdate><volume>54</volume><issue>6</issue><spage>1967</spage><epage>1976</epage><pages>1967-1976</pages><issn>1053-1807</issn><eissn>1522-2586</eissn><abstract>Background
Adjusting abnormal glutamate neurotransmission is a crucial mechanism in the treatment of depression. However, few non‐invasive techniques could effectively detect changes in glutamate neurotransmitters, and no consensus exists on whether glutamate could affect resting‐state function changes in depression.
Purpose
To study the changes in glutamate chemical exchange saturation transfer (GluCEST) value in the hippocampus of rat model exposed to chronic unpredictable mild stress (CUMS), and to explore the effect of this change on the activity of hippocampal glutamatergic neurons.
Study Type
Prospective animal study.
Animal Model
Twenty male Sprague–Dawley rats (200–300 g).
Field Strength/Sequence
7.0 T scanner. Fat rapid acquisition relaxation enhancement sequence for GluCEST, and echo planner imaging sequence for resting‐state functional magnetic resonance imaging (rs_fMRI).
Assessment
Rats were divided into two groups: CUMS group (N = 10) and control group (CTRL, N = 10). The magnetization transfer ratio asymmetry analysis was used to quantify the GluCEST data, and evaluate the rs_fMRI data through the amplitude of low‐frequency fluctuation (ALFF) and regional homogeneity (ReHo) analysis.
Statistical Tests
A t‐test was used to compare the difference in GluCEST or rs_fMRI between CUMS and CTRL groups. Spearman's correlation was applied to explore the correlation between GluCEST values and abnormal fMRI values in hippocampus. Statistical significance was set at P < 0.05.
Results
The GluCEST value in the left hippocampus has changed significantly (3.3 ± 0.3 [CUMS] vs. 3.9 ± 0.4 [CTRL], P < 0.05). In addition, the GluCEST value was significantly positively correlated with the ALFF values (r = 0.5, P < 0. 05, df = 7) and negatively correlated with the ReHo values (r = −0.6, P < 0.05, df = 7).
Data Conclusion
GluCEST technique has the feasibility of mapping glutamate changes in rat depression. Glutamate neurotransmitters are important factors affecting the abnormal function of neural activity.
Level of Evidence
2
Technical Efficacy
Stage 1</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>34291854</pmid><doi>10.1002/jmri.27850</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-4469-3390</orcidid><orcidid>https://orcid.org/0000-0002-1531-2520</orcidid></addata></record> |
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| ispartof | Journal of magnetic resonance imaging, 2021-12, Vol.54 (6), p.1967-1976 |
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| subjects | Animal models Animals Brain Brain Mapping Change detection chemical exchange saturation transfer Correlation depression Depression - diagnostic imaging Field strength Functional magnetic resonance imaging glutamate Glutamatergic transmission Glutamic Acid Hippocampus Hippocampus - diagnostic imaging Homogeneity Magnetic Resonance Imaging Male Neurotransmission Neurotransmitters Pilot Projects Prospective Studies Rats Rats, Sprague-Dawley resting‐state functional magnetic resonance imaging Saturation Statistical analysis Statistical tests |
| title | Glutamate Chemical Exchange Saturation Transfer Imaging and Functional Alterations of Hippocampus in Rat Depression Model: A Pilot Study |
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