Optimizing the Expression and Solubilization of an E. coli-Produced Leukemia Inhibitory Factor for Anti-LIF Antibody Production and Use Thereof for Contraception in Mice

Leukemia inhibitory factor (LIF) is an essential cytokine for blastocyst implantation. This study evaluated the effect of LIF inhibition on the blockage of embryo implantation. A truncated mouse LIF (tmLIF) was designed and expressed in E. coli . The protein expression was optimized using different...

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Veröffentlicht in:	Molecular biotechnology 2021-12, Vol.63 (12), p.1169-1182
Hauptverfasser:	Mehri, Nahid, Jamshidizad, Abbas, Ghanei, Zahra, Karkhane, Ali-Asghar, Shamsara, Mehdi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies Antibodies - administration & dosage Antibodies - pharmacology Biochemistry Biological Techniques Biotechnology Cell Biology Chemistry Chemistry and Materials Science Contraception Contraceptives Culture media Culture Media - chemistry Cytokines E coli Embryo Implantation - drug effects Embryos Escherichia coli - genetics Escherichia coli - growth & development Female Gene expression Gene Expression Profiling Genes Human Genetics Immunization Implantation In vivo methods and tests Inclusion bodies Injection Leukemia Leukemia inhibitory factor Leukemia Inhibitory Factor - genetics Leukemia Inhibitory Factor - immunology Leukemia Inhibitory Factor - metabolism Mice MUC1 gene Mutation Offspring Original Paper Pregnancy Protein Science Protein Stability Proteins Recombinant Proteins - administration & dosage Recombinant Proteins - chemistry Recombinant Proteins - immunology Recombinant Proteins - pharmacology Serum Solubility Solubilization Urea Uterus Uterus - chemistry
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