Expression of nuclear progesterone receptor, progesterone receptor membrane components 1 and 2 and prostaglandin-endoperoxide synthase 2 in the endometrium and oviduct of spontaneously ovulating cats
Although ovulations not followed by pregnancy occur regularly in cats, differences in endometrial function between cats in the luteal and non-luteal phase have not been studied so far. Progesterone exerts its effects through a nuclear progesterone receptor (PGR) and via cell-membrane bound receptors...
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| Veröffentlicht in: | Theriogenology 2021-09, Vol.172, p.200-206 |
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| creator | Binder, C. Aurich, J. Scarlet, D. Reichart, U. Walter, I. Aurich, C. |
| description | Although ovulations not followed by pregnancy occur regularly in cats, differences in endometrial function between cats in the luteal and non-luteal phase have not been studied so far. Progesterone exerts its effects through a nuclear progesterone receptor (PGR) and via cell-membrane bound receptors referred to as progesterone receptor membrane component (PGRMC) 1 and 2. Progesterone receptor expression is regulated by gonadal steroid hormones and therefore may change throughout the oestrous cycle. Protein expression of PGR, PGRMC-1 and 2 and prostaglandin-endoperoxide synthase 2 (PTGS2) was analysed in the endometrium and oviduct of non-pregnant female cats in the follicular (n = 8) and luteal phase (n = 9). We hypothesized that the presence of corpora lutea (CL) is associated with downregulation of progesterone receptors and PTGS2. Cells of the luminal endometrial epithelium, endometrial stroma and oviductal epithelium were assessed by immunohistochemistry. The PGR protein expression was more pronounced in the endometrial epithelium than stroma (p |
| doi_str_mv | 10.1016/j.theriogenology.2021.06.017 |
| format | Article |
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•Progesterone receptors and PTGS2 were studied for the first time in the uterus and oviduct of non-pregnant cats with and without CL.•Although cats express a prolonged non-pregnant luteal phase, progesterone receptor and PTGS2 abundance resemble findings in cyclic species.•Progesterone receptor expression was downregulated in cats with a CL.•In the endometrial epithelium, PGRMC-1 expression was reduced in cats with CL.•PTGS2 abundance in the endometrial and oviductal was reduced in cats with CL.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2021.06.017</identifier><language>eng</language><publisher>Elsevier Inc</publisher><subject>Cat ; Endometrium ; Oviduct ; Progesterone receptor ; PTGS2</subject><ispartof>Theriogenology, 2021-09, Vol.172, p.200-206</ispartof><rights>2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-8183031a7bcace84d9d47149da440570644db8fc848a11c032d1fd6ce6e2a1de3</citedby><cites>FETCH-LOGICAL-c359t-8183031a7bcace84d9d47149da440570644db8fc848a11c032d1fd6ce6e2a1de3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2021.06.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids></links><search><creatorcontrib>Binder, C.</creatorcontrib><creatorcontrib>Aurich, J.</creatorcontrib><creatorcontrib>Scarlet, D.</creatorcontrib><creatorcontrib>Reichart, U.</creatorcontrib><creatorcontrib>Walter, I.</creatorcontrib><creatorcontrib>Aurich, C.</creatorcontrib><title>Expression of nuclear progesterone receptor, progesterone receptor membrane components 1 and 2 and prostaglandin-endoperoxide synthase 2 in the endometrium and oviduct of spontaneously ovulating cats</title><title>Theriogenology</title><description>Although ovulations not followed by pregnancy occur regularly in cats, differences in endometrial function between cats in the luteal and non-luteal phase have not been studied so far. Progesterone exerts its effects through a nuclear progesterone receptor (PGR) and via cell-membrane bound receptors referred to as progesterone receptor membrane component (PGRMC) 1 and 2. Progesterone receptor expression is regulated by gonadal steroid hormones and therefore may change throughout the oestrous cycle. Protein expression of PGR, PGRMC-1 and 2 and prostaglandin-endoperoxide synthase 2 (PTGS2) was analysed in the endometrium and oviduct of non-pregnant female cats in the follicular (n = 8) and luteal phase (n = 9). We hypothesized that the presence of corpora lutea (CL) is associated with downregulation of progesterone receptors and PTGS2. Cells of the luminal endometrial epithelium, endometrial stroma and oviductal epithelium were assessed by immunohistochemistry. The PGR protein expression was more pronounced in the endometrial epithelium than stroma (p < 0.001) and less pronounced in cats with a CL than without CL (p < 0.001) but did not differ between groups in the oviduct. The PTGS2 was localized only in the endometrial and oviductal epithelium and its expression was reduced in cats with CL (p = 0.001). In the endometrial epithelium, PGRMC-1 expression was reduced in cats with CL (p < 0.05). Expression of PGRMC-2 was highest in the endometrial epithelium and lowest in the endometrial stroma (p = 0.01) but did not differ between cats with and without CL. In conclusion, progesterone receptor and PTGS2 downregulation in the female cat closely resembles findings in other spontaneously ovulating domestic animal species.
•Progesterone receptors and PTGS2 were studied for the first time in the uterus and oviduct of non-pregnant cats with and without CL.•Although cats express a prolonged non-pregnant luteal phase, progesterone receptor and PTGS2 abundance resemble findings in cyclic species.•Progesterone receptor expression was downregulated in cats with a CL.•In the endometrial epithelium, PGRMC-1 expression was reduced in cats with CL.•PTGS2 abundance in the endometrial and oviductal was reduced in cats with CL.</description><subject>Cat</subject><subject>Endometrium</subject><subject>Oviduct</subject><subject>Progesterone receptor</subject><subject>PTGS2</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqNUcFu1DAQtRBILIV_8IEDB5J64qyTSFxQ1UKlSlxA4mZ57cnWq8QOtlN1v7C_xWyXCxIHLrY1b97zvHmMvQdRgwB1eajLPSYf9xjiFPfHuhEN1ELVAroXbAN9N1SykfCSbYQYZKUG-Pmavcn5IISQSsGGPV0_Lglz9jHwOPKw2glN4ksi0VwwxYA8ocWlxPTx32U-47xLhio2zgshoWQO3ATHm-eTWLmY_URvHyoMLi6k8Ogd8nwM5d5kpE4fOLnhJ3jGkvw6P5Pjg3erLafhMokX-ieueToSsE6m-LDn1pT8lr0azZTx3Z_7gv24uf5-9bW6-_bl9urzXWXldihVD70UEky3s8Zi37rBtR20gzNtK7adUG3rdv1o-7Y3AFbIxsHolEWFjQGH8oJ9OOuSqV8r7ULPPlucpvNcutlum6FX0AG1fjq3WvKfE456SX426ahB6FN--qD_zk-f8tNCacqP6DdnOpKdB49JZ-sxWHSeNl-0i_7_hH4DkNe0iw</recordid><startdate>20210915</startdate><enddate>20210915</enddate><creator>Binder, C.</creator><creator>Aurich, J.</creator><creator>Scarlet, D.</creator><creator>Reichart, U.</creator><creator>Walter, I.</creator><creator>Aurich, C.</creator><general>Elsevier Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20210915</creationdate><title>Expression of nuclear progesterone receptor, progesterone receptor membrane components 1 and 2 and prostaglandin-endoperoxide synthase 2 in the endometrium and oviduct of spontaneously ovulating cats</title><author>Binder, C. ; Aurich, J. ; Scarlet, D. ; Reichart, U. ; Walter, I. ; Aurich, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-8183031a7bcace84d9d47149da440570644db8fc848a11c032d1fd6ce6e2a1de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Cat</topic><topic>Endometrium</topic><topic>Oviduct</topic><topic>Progesterone receptor</topic><topic>PTGS2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Binder, C.</creatorcontrib><creatorcontrib>Aurich, J.</creatorcontrib><creatorcontrib>Scarlet, D.</creatorcontrib><creatorcontrib>Reichart, U.</creatorcontrib><creatorcontrib>Walter, I.</creatorcontrib><creatorcontrib>Aurich, C.</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Binder, C.</au><au>Aurich, J.</au><au>Scarlet, D.</au><au>Reichart, U.</au><au>Walter, I.</au><au>Aurich, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of nuclear progesterone receptor, progesterone receptor membrane components 1 and 2 and prostaglandin-endoperoxide synthase 2 in the endometrium and oviduct of spontaneously ovulating cats</atitle><jtitle>Theriogenology</jtitle><date>2021-09-15</date><risdate>2021</risdate><volume>172</volume><spage>200</spage><epage>206</epage><pages>200-206</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>Although ovulations not followed by pregnancy occur regularly in cats, differences in endometrial function between cats in the luteal and non-luteal phase have not been studied so far. Progesterone exerts its effects through a nuclear progesterone receptor (PGR) and via cell-membrane bound receptors referred to as progesterone receptor membrane component (PGRMC) 1 and 2. Progesterone receptor expression is regulated by gonadal steroid hormones and therefore may change throughout the oestrous cycle. Protein expression of PGR, PGRMC-1 and 2 and prostaglandin-endoperoxide synthase 2 (PTGS2) was analysed in the endometrium and oviduct of non-pregnant female cats in the follicular (n = 8) and luteal phase (n = 9). We hypothesized that the presence of corpora lutea (CL) is associated with downregulation of progesterone receptors and PTGS2. Cells of the luminal endometrial epithelium, endometrial stroma and oviductal epithelium were assessed by immunohistochemistry. The PGR protein expression was more pronounced in the endometrial epithelium than stroma (p < 0.001) and less pronounced in cats with a CL than without CL (p < 0.001) but did not differ between groups in the oviduct. The PTGS2 was localized only in the endometrial and oviductal epithelium and its expression was reduced in cats with CL (p = 0.001). In the endometrial epithelium, PGRMC-1 expression was reduced in cats with CL (p < 0.05). Expression of PGRMC-2 was highest in the endometrial epithelium and lowest in the endometrial stroma (p = 0.01) but did not differ between cats with and without CL. In conclusion, progesterone receptor and PTGS2 downregulation in the female cat closely resembles findings in other spontaneously ovulating domestic animal species.
•Progesterone receptors and PTGS2 were studied for the first time in the uterus and oviduct of non-pregnant cats with and without CL.•Although cats express a prolonged non-pregnant luteal phase, progesterone receptor and PTGS2 abundance resemble findings in cyclic species.•Progesterone receptor expression was downregulated in cats with a CL.•In the endometrial epithelium, PGRMC-1 expression was reduced in cats with CL.•PTGS2 abundance in the endometrial and oviductal was reduced in cats with CL.</abstract><pub>Elsevier Inc</pub><doi>10.1016/j.theriogenology.2021.06.017</doi><tpages>7</tpages></addata></record> |
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| subjects | Cat Endometrium Oviduct Progesterone receptor PTGS2 |
| title | Expression of nuclear progesterone receptor, progesterone receptor membrane components 1 and 2 and prostaglandin-endoperoxide synthase 2 in the endometrium and oviduct of spontaneously ovulating cats |
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