The influence of calcium phosphate ceramics and glass-ceramics on cultured cells and their surrounding media
The cytotoxicity of hydroxyapatite (HA), tricalcium phosphate (TCP), and two types of phosphate (TCP), and two types of apatite‐containing glass‐ceramics designated A · W‐GC and A · W · CP‐GC was evaluated by the direct contact method using V79 cells. Compatibility of the cells was assessed as a fun...
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| Veröffentlicht in: | Journal of biomedical materials research 1989-09, Vol.23 (9), p.1049-1066 |
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| container_title | Journal of biomedical materials research |
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| creator | Hyakuna, Katsufumi Yamamuro, Takao Kotoura, Yoshihiko Kakutani, Yoshiaki Kitsugi, Toshiaki Takagi, Haruki Oka, Masanori Kokubo, Tadashi |
| description | The cytotoxicity of hydroxyapatite (HA), tricalcium phosphate (TCP), and two types of phosphate (TCP), and two types of apatite‐containing glass‐ceramics designated A · W‐GC and A · W · CP‐GC was evaluated by the direct contact method using V79 cells. Compatibility of the cells was assessed as a function of cellular attachment and subsequent growth using scanning electron microscopy. Although all of the tested materials have surfaces available for cellular attachment, cell growth was markedly inhibited by HA sintered at 600°C and 900°C, and slightly inhibited by HA sintered at 1200°C, TCP and the glass‐ceramics. In attempts to clarify the cause of this toxicity, the interactions of the ceramics with culture medium was assessed by measurement of calcium (Ca), inorganic phosphate (P), magnesium (Mg), pH and albumin content in the medium. The grade of toxicity corresponded to the degree of reduction of Ca, P, and albumin in the medium. Furthermore, surface reactions of the ceramics were demonstrated to vary in both mode and degree depending on their chemical structure and level of microporosity. Thus, the surface reactivity of ceramic materials should be always taken into account in discussing their biocompatibility in vivo. |
| doi_str_mv | 10.1002/jbm.820230906 |
| format | Article |
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Compatibility of the cells was assessed as a function of cellular attachment and subsequent growth using scanning electron microscopy. Although all of the tested materials have surfaces available for cellular attachment, cell growth was markedly inhibited by HA sintered at 600°C and 900°C, and slightly inhibited by HA sintered at 1200°C, TCP and the glass‐ceramics. In attempts to clarify the cause of this toxicity, the interactions of the ceramics with culture medium was assessed by measurement of calcium (Ca), inorganic phosphate (P), magnesium (Mg), pH and albumin content in the medium. The grade of toxicity corresponded to the degree of reduction of Ca, P, and albumin in the medium. Furthermore, surface reactions of the ceramics were demonstrated to vary in both mode and degree depending on their chemical structure and level of microporosity. Thus, the surface reactivity of ceramic materials should be always taken into account in discussing their biocompatibility in vivo.</description><identifier>ISSN: 0021-9304</identifier><identifier>EISSN: 1097-4636</identifier><identifier>DOI: 10.1002/jbm.820230906</identifier><identifier>PMID: 2777833</identifier><identifier>CODEN: JBMRBG</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Animals ; Biocompatible Materials ; Biological and medical sciences ; Calcium Phosphates ; Cell Adhesion - drug effects ; Cell Communication - drug effects ; Cell Division - drug effects ; Cells, Cultured ; Ceramics ; Cricetinae ; Cricetulus ; Culture Media ; Fibroblasts - drug effects ; Fibroblasts - physiology ; Glass ; Hydrogen-Ion Concentration ; Materials Testing ; Medical sciences ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; Serum Albumin - analysis ; Technology. Biomaterials. Equipments. Material. Instrumentation</subject><ispartof>Journal of biomedical materials research, 1989-09, Vol.23 (9), p.1049-1066</ispartof><rights>Copyright © 1989 John Wiley & Sons, Inc.</rights><rights>1990 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4346-c3f16fe93260c7348dec754a4c480d83efc14af25b8305f87f3888674f0d2bf13</citedby><cites>FETCH-LOGICAL-c4346-c3f16fe93260c7348dec754a4c480d83efc14af25b8305f87f3888674f0d2bf13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbm.820230906$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbm.820230906$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6596733$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2777833$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hyakuna, Katsufumi</creatorcontrib><creatorcontrib>Yamamuro, Takao</creatorcontrib><creatorcontrib>Kotoura, Yoshihiko</creatorcontrib><creatorcontrib>Kakutani, Yoshiaki</creatorcontrib><creatorcontrib>Kitsugi, Toshiaki</creatorcontrib><creatorcontrib>Takagi, Haruki</creatorcontrib><creatorcontrib>Oka, Masanori</creatorcontrib><creatorcontrib>Kokubo, Tadashi</creatorcontrib><title>The influence of calcium phosphate ceramics and glass-ceramics on cultured cells and their surrounding media</title><title>Journal of biomedical materials research</title><addtitle>J. Biomed. Mater. Res</addtitle><description>The cytotoxicity of hydroxyapatite (HA), tricalcium phosphate (TCP), and two types of phosphate (TCP), and two types of apatite‐containing glass‐ceramics designated A · W‐GC and A · W · CP‐GC was evaluated by the direct contact method using V79 cells. Compatibility of the cells was assessed as a function of cellular attachment and subsequent growth using scanning electron microscopy. Although all of the tested materials have surfaces available for cellular attachment, cell growth was markedly inhibited by HA sintered at 600°C and 900°C, and slightly inhibited by HA sintered at 1200°C, TCP and the glass‐ceramics. In attempts to clarify the cause of this toxicity, the interactions of the ceramics with culture medium was assessed by measurement of calcium (Ca), inorganic phosphate (P), magnesium (Mg), pH and albumin content in the medium. The grade of toxicity corresponded to the degree of reduction of Ca, P, and albumin in the medium. Furthermore, surface reactions of the ceramics were demonstrated to vary in both mode and degree depending on their chemical structure and level of microporosity. Thus, the surface reactivity of ceramic materials should be always taken into account in discussing their biocompatibility in vivo.</description><subject>Animals</subject><subject>Biocompatible Materials</subject><subject>Biological and medical sciences</subject><subject>Calcium Phosphates</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Communication - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Cells, Cultured</subject><subject>Ceramics</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Culture Media</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - physiology</subject><subject>Glass</subject><subject>Hydrogen-Ion Concentration</subject><subject>Materials Testing</subject><subject>Medical sciences</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>Serum Albumin - analysis</subject><subject>Technology. Biomaterials. Equipments. Material. 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Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>Serum Albumin - analysis</topic><topic>Technology. Biomaterials. Equipments. Material. Instrumentation</topic><toplevel>online_resources</toplevel><creatorcontrib>Hyakuna, Katsufumi</creatorcontrib><creatorcontrib>Yamamuro, Takao</creatorcontrib><creatorcontrib>Kotoura, Yoshihiko</creatorcontrib><creatorcontrib>Kakutani, Yoshiaki</creatorcontrib><creatorcontrib>Kitsugi, Toshiaki</creatorcontrib><creatorcontrib>Takagi, Haruki</creatorcontrib><creatorcontrib>Oka, Masanori</creatorcontrib><creatorcontrib>Kokubo, Tadashi</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Journal of biomedical materials research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hyakuna, Katsufumi</au><au>Yamamuro, Takao</au><au>Kotoura, Yoshihiko</au><au>Kakutani, Yoshiaki</au><au>Kitsugi, Toshiaki</au><au>Takagi, Haruki</au><au>Oka, Masanori</au><au>Kokubo, Tadashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The influence of calcium phosphate ceramics and glass-ceramics on cultured cells and their surrounding media</atitle><jtitle>Journal of biomedical materials research</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>1989-09</date><risdate>1989</risdate><volume>23</volume><issue>9</issue><spage>1049</spage><epage>1066</epage><pages>1049-1066</pages><issn>0021-9304</issn><eissn>1097-4636</eissn><coden>JBMRBG</coden><abstract>The cytotoxicity of hydroxyapatite (HA), tricalcium phosphate (TCP), and two types of phosphate (TCP), and two types of apatite‐containing glass‐ceramics designated A · W‐GC and A · W · CP‐GC was evaluated by the direct contact method using V79 cells. Compatibility of the cells was assessed as a function of cellular attachment and subsequent growth using scanning electron microscopy. Although all of the tested materials have surfaces available for cellular attachment, cell growth was markedly inhibited by HA sintered at 600°C and 900°C, and slightly inhibited by HA sintered at 1200°C, TCP and the glass‐ceramics. In attempts to clarify the cause of this toxicity, the interactions of the ceramics with culture medium was assessed by measurement of calcium (Ca), inorganic phosphate (P), magnesium (Mg), pH and albumin content in the medium. The grade of toxicity corresponded to the degree of reduction of Ca, P, and albumin in the medium. Furthermore, surface reactions of the ceramics were demonstrated to vary in both mode and degree depending on their chemical structure and level of microporosity. Thus, the surface reactivity of ceramic materials should be always taken into account in discussing their biocompatibility in vivo.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>2777833</pmid><doi>10.1002/jbm.820230906</doi><tpages>18</tpages></addata></record> |
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| ispartof | Journal of biomedical materials research, 1989-09, Vol.23 (9), p.1049-1066 |
| issn | 0021-9304 1097-4636 |
| language | eng |
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| source | MEDLINE; Wiley Journals |
| subjects | Animals Biocompatible Materials Biological and medical sciences Calcium Phosphates Cell Adhesion - drug effects Cell Communication - drug effects Cell Division - drug effects Cells, Cultured Ceramics Cricetinae Cricetulus Culture Media Fibroblasts - drug effects Fibroblasts - physiology Glass Hydrogen-Ion Concentration Materials Testing Medical sciences Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) Serum Albumin - analysis Technology. Biomaterials. Equipments. Material. Instrumentation |
| title | The influence of calcium phosphate ceramics and glass-ceramics on cultured cells and their surrounding media |
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