Genetic variability of Edwardsiella piscicida isolates from Mississippi catfish aquaculture with an assessment of virulence in channel and channel × blue hybrid catfish
The bacterium Edwardsiella piscicida causes significant losses in global aquaculture, particularly channel (Ictalurus punctatus) × blue (I. furcatus) hybrid catfish cultured in the south‐eastern United States. Emergence of E. piscicida in hybrid catfish is worrisome given current industry trends tow...
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| Veröffentlicht in: | Journal of fish diseases 2021-11, Vol.44 (11), p.1725-1751 |
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| creator | López‐Porras, Adrián Griffin, Matt J. Armwood, Abigail R. Camus, Alvin C. Waldbieser, Geoffrey C. Ware, Cynthia Richardson, Bradley Greenway, Terrence E. Rosser, Thomas Graham Aarattuthodiyil, Suja Wise, David J. |
| description | The bacterium Edwardsiella piscicida causes significant losses in global aquaculture, particularly channel (Ictalurus punctatus) × blue (I. furcatus) hybrid catfish cultured in the south‐eastern United States. Emergence of E. piscicida in hybrid catfish is worrisome given current industry trends towards increased hybrid production. The project objectives were to assess intraspecific genetic variability of E. piscicida isolates recovered from diseased channel and hybrid catfish in Mississippi; and determine virulence associations among genetic variants. Repetitive extragenic palindromic sequence‐based PCR (rep‐PCR) using ERIC I and II primers was used to screen 158 E. piscicida diagnostic case isolates. A subsample of 39 E. piscicida isolates, representing predominant rep‐PCR profiles, was further characterized using BOX and (GTG)5 rep‐PCR primers, virulence gene assessment and multilocus sequence analysis (MLSA) targeting housekeeping genes gyrb, pgi and phoU. The MLSA provided greater resolution than rep‐PCR, revealing 5 discrete phylogroups that correlated similarly with virulence gene profiles. Virulence assessments using E. piscicida representatives from each MLSA group resulted in 14‐day cumulative mortality ranging from 22% to 54% and 63 to 72% in channel and hybrid fingerlings, respectively. Across all phylogroups, mortality was higher in hybrid catfish (p |
| doi_str_mv | 10.1111/jfd.13491 |
| format | Article |
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Emergence of E. piscicida in hybrid catfish is worrisome given current industry trends towards increased hybrid production. The project objectives were to assess intraspecific genetic variability of E. piscicida isolates recovered from diseased channel and hybrid catfish in Mississippi; and determine virulence associations among genetic variants. Repetitive extragenic palindromic sequence‐based PCR (rep‐PCR) using ERIC I and II primers was used to screen 158 E. piscicida diagnostic case isolates. A subsample of 39 E. piscicida isolates, representing predominant rep‐PCR profiles, was further characterized using BOX and (GTG)5 rep‐PCR primers, virulence gene assessment and multilocus sequence analysis (MLSA) targeting housekeeping genes gyrb, pgi and phoU. The MLSA provided greater resolution than rep‐PCR, revealing 5 discrete phylogroups that correlated similarly with virulence gene profiles. Virulence assessments using E. piscicida representatives from each MLSA group resulted in 14‐day cumulative mortality ranging from 22% to 54% and 63 to 72% in channel and hybrid fingerlings, respectively. Across all phylogroups, mortality was higher in hybrid catfish (p < .05), supporting previous work indicating E. piscicida is an emerging threat to hybrid catfish aquaculture in the south‐eastern United States.</description><identifier>ISSN: 0140-7775</identifier><identifier>EISSN: 1365-2761</identifier><identifier>DOI: 10.1111/jfd.13491</identifier><language>eng</language><publisher>Oxford: Blackwell Publishing Ltd</publisher><subject>Aquaculture ; Catfish ; Edwardsiella piscicida ; edwardsiellosis ; Fingerlings ; Freshwater fishes ; Genes ; Genetic diversity ; Genetic variability ; Genetic variance ; hybrid catfish ; Ictalurus punctatus ; Mortality ; multilocus sequence analysis ; Nucleotide sequence ; PCR ; Primers ; rep‐PCR ; Sequence analysis ; Sequencing ; Variability ; Virulence</subject><ispartof>Journal of fish diseases, 2021-11, Vol.44 (11), p.1725-1751</ispartof><rights>2021 John Wiley & Sons Ltd</rights><rights>Copyright © 2021 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2451-4456ce69adddab4606a7f09f3bcc3513ae7a7a231905bfce96f93649048190123</citedby><cites>FETCH-LOGICAL-c2451-4456ce69adddab4606a7f09f3bcc3513ae7a7a231905bfce96f93649048190123</cites><orcidid>0000-0002-6783-1509 ; 0000-0002-7920-3446 ; 0000-0002-5695-6875</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjfd.13491$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjfd.13491$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids></links><search><creatorcontrib>López‐Porras, Adrián</creatorcontrib><creatorcontrib>Griffin, Matt J.</creatorcontrib><creatorcontrib>Armwood, Abigail R.</creatorcontrib><creatorcontrib>Camus, Alvin C.</creatorcontrib><creatorcontrib>Waldbieser, Geoffrey C.</creatorcontrib><creatorcontrib>Ware, Cynthia</creatorcontrib><creatorcontrib>Richardson, Bradley</creatorcontrib><creatorcontrib>Greenway, Terrence E.</creatorcontrib><creatorcontrib>Rosser, Thomas Graham</creatorcontrib><creatorcontrib>Aarattuthodiyil, Suja</creatorcontrib><creatorcontrib>Wise, David J.</creatorcontrib><title>Genetic variability of Edwardsiella piscicida isolates from Mississippi catfish aquaculture with an assessment of virulence in channel and channel × blue hybrid catfish</title><title>Journal of fish diseases</title><description>The bacterium Edwardsiella piscicida causes significant losses in global aquaculture, particularly channel (Ictalurus punctatus) × blue (I. furcatus) hybrid catfish cultured in the south‐eastern United States. Emergence of E. piscicida in hybrid catfish is worrisome given current industry trends towards increased hybrid production. The project objectives were to assess intraspecific genetic variability of E. piscicida isolates recovered from diseased channel and hybrid catfish in Mississippi; and determine virulence associations among genetic variants. Repetitive extragenic palindromic sequence‐based PCR (rep‐PCR) using ERIC I and II primers was used to screen 158 E. piscicida diagnostic case isolates. A subsample of 39 E. piscicida isolates, representing predominant rep‐PCR profiles, was further characterized using BOX and (GTG)5 rep‐PCR primers, virulence gene assessment and multilocus sequence analysis (MLSA) targeting housekeeping genes gyrb, pgi and phoU. The MLSA provided greater resolution than rep‐PCR, revealing 5 discrete phylogroups that correlated similarly with virulence gene profiles. Virulence assessments using E. piscicida representatives from each MLSA group resulted in 14‐day cumulative mortality ranging from 22% to 54% and 63 to 72% in channel and hybrid fingerlings, respectively. Across all phylogroups, mortality was higher in hybrid catfish (p < .05), supporting previous work indicating E. piscicida is an emerging threat to hybrid catfish aquaculture in the south‐eastern United States.</description><subject>Aquaculture</subject><subject>Catfish</subject><subject>Edwardsiella piscicida</subject><subject>edwardsiellosis</subject><subject>Fingerlings</subject><subject>Freshwater fishes</subject><subject>Genes</subject><subject>Genetic diversity</subject><subject>Genetic variability</subject><subject>Genetic variance</subject><subject>hybrid catfish</subject><subject>Ictalurus punctatus</subject><subject>Mortality</subject><subject>multilocus sequence analysis</subject><subject>Nucleotide sequence</subject><subject>PCR</subject><subject>Primers</subject><subject>rep‐PCR</subject><subject>Sequence analysis</subject><subject>Sequencing</subject><subject>Variability</subject><subject>Virulence</subject><issn>0140-7775</issn><issn>1365-2761</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kc9u1DAQxi0EEkvhwBtY4gKHtHYc28kRlf4BteIC52hij7VeeZPUTrraW688AU_Rp-ib9ElwWHqphDWyR6PffLK-j5D3nB3zfE42zh5zUTX8BVlxoWRRasVfkhXjFSu01vI1eZPShjGuJVcrcn-BPU7e0FuIHjof_LSng6NndgfRJo8hAB19Mt54C9SnIcCEibo4bOm1T2mpcfTUwOR8WlO4mcHMYZoj0p2f8qCnkBKmtMV-WqRvfZwD9gap76lZQ99jyJR96h_vfj38zlcXZqTrfRe9fVJ_S145CAnf_XuPyM_zsx-nl8XV94uvp5-vClNWkhdVJZVB1YC1FrpKMQXascaJzhghuQDUoKEUvGGycwYb5RqhqoZVdR7xUhyRjwfdMQ43M6ap3WYLFi96HObUllIyVWpe1xn98AzdDHPs8-8yVbNa1UItgp8OlIlDShFdO0a_hbhvOWuX4NocXPs3uMyeHNidD7j_P9h-O_9y2PgDi2Gf7Q</recordid><startdate>202111</startdate><enddate>202111</enddate><creator>López‐Porras, Adrián</creator><creator>Griffin, Matt J.</creator><creator>Armwood, Abigail R.</creator><creator>Camus, Alvin C.</creator><creator>Waldbieser, Geoffrey C.</creator><creator>Ware, Cynthia</creator><creator>Richardson, Bradley</creator><creator>Greenway, Terrence E.</creator><creator>Rosser, Thomas Graham</creator><creator>Aarattuthodiyil, Suja</creator><creator>Wise, David J.</creator><general>Blackwell Publishing Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TN</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6783-1509</orcidid><orcidid>https://orcid.org/0000-0002-7920-3446</orcidid><orcidid>https://orcid.org/0000-0002-5695-6875</orcidid></search><sort><creationdate>202111</creationdate><title>Genetic variability of Edwardsiella piscicida isolates from Mississippi catfish aquaculture with an assessment of virulence in channel and channel × blue hybrid catfish</title><author>López‐Porras, Adrián ; Griffin, Matt J. ; Armwood, Abigail R. ; Camus, Alvin C. ; Waldbieser, Geoffrey C. ; Ware, Cynthia ; Richardson, Bradley ; Greenway, Terrence E. ; Rosser, Thomas Graham ; Aarattuthodiyil, Suja ; Wise, David J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2451-4456ce69adddab4606a7f09f3bcc3513ae7a7a231905bfce96f93649048190123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aquaculture</topic><topic>Catfish</topic><topic>Edwardsiella piscicida</topic><topic>edwardsiellosis</topic><topic>Fingerlings</topic><topic>Freshwater fishes</topic><topic>Genes</topic><topic>Genetic diversity</topic><topic>Genetic variability</topic><topic>Genetic variance</topic><topic>hybrid catfish</topic><topic>Ictalurus punctatus</topic><topic>Mortality</topic><topic>multilocus sequence analysis</topic><topic>Nucleotide sequence</topic><topic>PCR</topic><topic>Primers</topic><topic>rep‐PCR</topic><topic>Sequence analysis</topic><topic>Sequencing</topic><topic>Variability</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>López‐Porras, Adrián</creatorcontrib><creatorcontrib>Griffin, Matt J.</creatorcontrib><creatorcontrib>Armwood, Abigail R.</creatorcontrib><creatorcontrib>Camus, Alvin C.</creatorcontrib><creatorcontrib>Waldbieser, Geoffrey C.</creatorcontrib><creatorcontrib>Ware, Cynthia</creatorcontrib><creatorcontrib>Richardson, Bradley</creatorcontrib><creatorcontrib>Greenway, Terrence E.</creatorcontrib><creatorcontrib>Rosser, Thomas Graham</creatorcontrib><creatorcontrib>Aarattuthodiyil, Suja</creatorcontrib><creatorcontrib>Wise, David J.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of fish diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>López‐Porras, Adrián</au><au>Griffin, Matt J.</au><au>Armwood, Abigail R.</au><au>Camus, Alvin C.</au><au>Waldbieser, Geoffrey C.</au><au>Ware, Cynthia</au><au>Richardson, Bradley</au><au>Greenway, Terrence E.</au><au>Rosser, Thomas Graham</au><au>Aarattuthodiyil, Suja</au><au>Wise, David J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic variability of Edwardsiella piscicida isolates from Mississippi catfish aquaculture with an assessment of virulence in channel and channel × blue hybrid catfish</atitle><jtitle>Journal of fish diseases</jtitle><date>2021-11</date><risdate>2021</risdate><volume>44</volume><issue>11</issue><spage>1725</spage><epage>1751</epage><pages>1725-1751</pages><issn>0140-7775</issn><eissn>1365-2761</eissn><abstract>The bacterium Edwardsiella piscicida causes significant losses in global aquaculture, particularly channel (Ictalurus punctatus) × blue (I. furcatus) hybrid catfish cultured in the south‐eastern United States. Emergence of E. piscicida in hybrid catfish is worrisome given current industry trends towards increased hybrid production. The project objectives were to assess intraspecific genetic variability of E. piscicida isolates recovered from diseased channel and hybrid catfish in Mississippi; and determine virulence associations among genetic variants. Repetitive extragenic palindromic sequence‐based PCR (rep‐PCR) using ERIC I and II primers was used to screen 158 E. piscicida diagnostic case isolates. A subsample of 39 E. piscicida isolates, representing predominant rep‐PCR profiles, was further characterized using BOX and (GTG)5 rep‐PCR primers, virulence gene assessment and multilocus sequence analysis (MLSA) targeting housekeeping genes gyrb, pgi and phoU. The MLSA provided greater resolution than rep‐PCR, revealing 5 discrete phylogroups that correlated similarly with virulence gene profiles. Virulence assessments using E. piscicida representatives from each MLSA group resulted in 14‐day cumulative mortality ranging from 22% to 54% and 63 to 72% in channel and hybrid fingerlings, respectively. Across all phylogroups, mortality was higher in hybrid catfish (p < .05), supporting previous work indicating E. piscicida is an emerging threat to hybrid catfish aquaculture in the south‐eastern United States.</abstract><cop>Oxford</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/jfd.13491</doi><tpages>27</tpages><orcidid>https://orcid.org/0000-0002-6783-1509</orcidid><orcidid>https://orcid.org/0000-0002-7920-3446</orcidid><orcidid>https://orcid.org/0000-0002-5695-6875</orcidid></addata></record> |
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| ispartof | Journal of fish diseases, 2021-11, Vol.44 (11), p.1725-1751 |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Aquaculture Catfish Edwardsiella piscicida edwardsiellosis Fingerlings Freshwater fishes Genes Genetic diversity Genetic variability Genetic variance hybrid catfish Ictalurus punctatus Mortality multilocus sequence analysis Nucleotide sequence PCR Primers rep‐PCR Sequence analysis Sequencing Variability Virulence |
| title | Genetic variability of Edwardsiella piscicida isolates from Mississippi catfish aquaculture with an assessment of virulence in channel and channel × blue hybrid catfish |
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