In vivo study of the angiogenesis potential of bone marrow-derived mesenchymal stem cell aggregates in their niche like environment
Background: Sufficient blood vessel formation in bioengineered tissues is essential in order to keep the viability of the organs. Impaired development of blood vasculatures results in failure of the implanted tissue. The cellular source which is seeded in the scaffold is one of the crucial factors i...
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| Veröffentlicht in: | International journal of artificial organs 2021-10, Vol.44 (10), p.727-733 |
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| container_title | International journal of artificial organs |
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| creator | Anajafi, Sara Ranjbar, Azam Torabi-Rahvar, Monireh Ahmadbeigi, Naser |
| description | Background:
Sufficient blood vessel formation in bioengineered tissues is essential in order to keep the viability of the organs. Impaired development of blood vasculatures results in failure of the implanted tissue. The cellular source which is seeded in the scaffold is one of the crucial factors involved in tissue engineering methods.
Materials and methods:
Considering the notable competence of Bone Marrow derived Mesenchymal Stem Cell aggregates for tissue engineering purposes, in this study BM-aggregates and expanded BM-MSCs were applied without any inductive agent or co-cultured cells, in order to investigate their own angiogenesis potency in vivo. BM-aggregates and BM-MSC were seeded in Poly-L Lactic acid (PLLA) scaffold and implanted in the peritoneal cavity of mice.
Result:
Immunohistochemistry results indicated that there was a significant difference (p |
| doi_str_mv | 10.1177/03913988211025538 |
| format | Article |
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Sufficient blood vessel formation in bioengineered tissues is essential in order to keep the viability of the organs. Impaired development of blood vasculatures results in failure of the implanted tissue. The cellular source which is seeded in the scaffold is one of the crucial factors involved in tissue engineering methods.
Materials and methods:
Considering the notable competence of Bone Marrow derived Mesenchymal Stem Cell aggregates for tissue engineering purposes, in this study BM-aggregates and expanded BM-MSCs were applied without any inductive agent or co-cultured cells, in order to investigate their own angiogenesis potency in vivo. BM-aggregates and BM-MSC were seeded in Poly-L Lactic acid (PLLA) scaffold and implanted in the peritoneal cavity of mice.
Result:
Immunohistochemistry results indicated that there was a significant difference (p < 0.050) in CD31+ cells between PLLA scaffolds contained cultured BM-MSC; PLLA scaffolds contained BM-aggregates and empty PLLA. According to morphological evidence, obvious connections with recipient vasculature and acceptable integration with surroundings were established in MSC and aggregate-seeded scaffolds.
Conclusion:
Our findings revealed cultured BM-MSC and BM-aggregates, capacity in order to develop numerous connections between PLLA scaffold and recipient’s vasculature which is crucial to the survival of tissues, and considerable tendency to develop constructs containing CD31+ endothelial cells which can contribute in vessel’s tube formation.</description><identifier>ISSN: 0391-3988</identifier><identifier>EISSN: 1724-6040</identifier><identifier>DOI: 10.1177/03913988211025538</identifier><identifier>PMID: 34250831</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Aggregates ; Angiogenesis ; Animals ; Bioengineering ; Blood vessels ; Bone Marrow ; Bone Marrow Cells ; Cell Differentiation ; Cells, Cultured ; Coculture Techniques ; Endothelial Cells ; Immunohistochemistry ; In vivo methods and tests ; Lactic acid ; Mesenchymal Stem Cells ; Mice ; Organs ; Peritoneum ; Polylactic acid ; Scaffolds ; Stem cells ; Tissue Engineering ; Tissue Scaffolds</subject><ispartof>International journal of artificial organs, 2021-10, Vol.44 (10), p.727-733</ispartof><rights>The Author(s) 2021</rights><rights>Copyright Wichtig Editore s.r.l. Oct 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c320t-f774430dcf02bc12071bc4e381fb1cb0e3ad8d252e89e69733c37f2b5f3364323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/03913988211025538$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/03913988211025538$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21819,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34250831$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anajafi, Sara</creatorcontrib><creatorcontrib>Ranjbar, Azam</creatorcontrib><creatorcontrib>Torabi-Rahvar, Monireh</creatorcontrib><creatorcontrib>Ahmadbeigi, Naser</creatorcontrib><title>In vivo study of the angiogenesis potential of bone marrow-derived mesenchymal stem cell aggregates in their niche like environment</title><title>International journal of artificial organs</title><addtitle>Int J Artif Organs</addtitle><description>Background:
Sufficient blood vessel formation in bioengineered tissues is essential in order to keep the viability of the organs. Impaired development of blood vasculatures results in failure of the implanted tissue. The cellular source which is seeded in the scaffold is one of the crucial factors involved in tissue engineering methods.
Materials and methods:
Considering the notable competence of Bone Marrow derived Mesenchymal Stem Cell aggregates for tissue engineering purposes, in this study BM-aggregates and expanded BM-MSCs were applied without any inductive agent or co-cultured cells, in order to investigate their own angiogenesis potency in vivo. BM-aggregates and BM-MSC were seeded in Poly-L Lactic acid (PLLA) scaffold and implanted in the peritoneal cavity of mice.
Result:
Immunohistochemistry results indicated that there was a significant difference (p < 0.050) in CD31+ cells between PLLA scaffolds contained cultured BM-MSC; PLLA scaffolds contained BM-aggregates and empty PLLA. According to morphological evidence, obvious connections with recipient vasculature and acceptable integration with surroundings were established in MSC and aggregate-seeded scaffolds.
Conclusion:
Our findings revealed cultured BM-MSC and BM-aggregates, capacity in order to develop numerous connections between PLLA scaffold and recipient’s vasculature which is crucial to the survival of tissues, and considerable tendency to develop constructs containing CD31+ endothelial cells which can contribute in vessel’s tube formation.</description><subject>Aggregates</subject><subject>Angiogenesis</subject><subject>Animals</subject><subject>Bioengineering</subject><subject>Blood vessels</subject><subject>Bone Marrow</subject><subject>Bone Marrow Cells</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Coculture Techniques</subject><subject>Endothelial Cells</subject><subject>Immunohistochemistry</subject><subject>In vivo methods and tests</subject><subject>Lactic acid</subject><subject>Mesenchymal Stem Cells</subject><subject>Mice</subject><subject>Organs</subject><subject>Peritoneum</subject><subject>Polylactic acid</subject><subject>Scaffolds</subject><subject>Stem cells</subject><subject>Tissue Engineering</subject><subject>Tissue Scaffolds</subject><issn>0391-3988</issn><issn>1724-6040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctKxDAUhoMoOl4ewI0E3LipJjnpZZYi3kBwo-uSpqc12iZj0o7M2hc3ZbyA4iqL8_1fDucn5JCzU87z_IzBnMO8KATnTKQpFBtkxnMhk4xJtklm0zyZgB2yG8IzYzyTMt0mOyBFygrgM_J-a-nSLB0Nw1ivqGvo8IRU2da4Fi0GE-jCDWgHo7ppWjmLtFfeu7ekRm-WWNMeA1r9tOojEgbsqcauo6ptPbZqwECNnazGU2t0tHfmBSnapfHO9lG9T7Ya1QU8-Hz3yOPV5cPFTXJ3f317cX6XaBBsSJo8lxJYrRsmKs0Fy3mlJULBm4rriiGouqhFKrCYYzbPATTkjajSBiCTIGCPnKy9C-9eRwxD2Zsw7aosujGU8YQsi_k0i-jxL_TZjd7G7SJVMEgFl5OQryntXQgem3LhTTzOquSsnBoq_zQUM0ef5rHqsf5OfFUSgdM1EFSLP9_-b_wAqniZdw</recordid><startdate>202110</startdate><enddate>202110</enddate><creator>Anajafi, Sara</creator><creator>Ranjbar, Azam</creator><creator>Torabi-Rahvar, Monireh</creator><creator>Ahmadbeigi, Naser</creator><general>SAGE Publications</general><general>Wichtig Editore s.r.l</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>202110</creationdate><title>In vivo study of the angiogenesis potential of bone marrow-derived mesenchymal stem cell aggregates in their niche like environment</title><author>Anajafi, Sara ; Ranjbar, Azam ; Torabi-Rahvar, Monireh ; Ahmadbeigi, Naser</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c320t-f774430dcf02bc12071bc4e381fb1cb0e3ad8d252e89e69733c37f2b5f3364323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aggregates</topic><topic>Angiogenesis</topic><topic>Animals</topic><topic>Bioengineering</topic><topic>Blood vessels</topic><topic>Bone Marrow</topic><topic>Bone Marrow Cells</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Coculture Techniques</topic><topic>Endothelial Cells</topic><topic>Immunohistochemistry</topic><topic>In vivo methods and tests</topic><topic>Lactic acid</topic><topic>Mesenchymal Stem Cells</topic><topic>Mice</topic><topic>Organs</topic><topic>Peritoneum</topic><topic>Polylactic acid</topic><topic>Scaffolds</topic><topic>Stem cells</topic><topic>Tissue Engineering</topic><topic>Tissue Scaffolds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anajafi, Sara</creatorcontrib><creatorcontrib>Ranjbar, Azam</creatorcontrib><creatorcontrib>Torabi-Rahvar, Monireh</creatorcontrib><creatorcontrib>Ahmadbeigi, Naser</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of artificial organs</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anajafi, Sara</au><au>Ranjbar, Azam</au><au>Torabi-Rahvar, Monireh</au><au>Ahmadbeigi, Naser</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo study of the angiogenesis potential of bone marrow-derived mesenchymal stem cell aggregates in their niche like environment</atitle><jtitle>International journal of artificial organs</jtitle><addtitle>Int J Artif Organs</addtitle><date>2021-10</date><risdate>2021</risdate><volume>44</volume><issue>10</issue><spage>727</spage><epage>733</epage><pages>727-733</pages><issn>0391-3988</issn><eissn>1724-6040</eissn><abstract>Background:
Sufficient blood vessel formation in bioengineered tissues is essential in order to keep the viability of the organs. Impaired development of blood vasculatures results in failure of the implanted tissue. The cellular source which is seeded in the scaffold is one of the crucial factors involved in tissue engineering methods.
Materials and methods:
Considering the notable competence of Bone Marrow derived Mesenchymal Stem Cell aggregates for tissue engineering purposes, in this study BM-aggregates and expanded BM-MSCs were applied without any inductive agent or co-cultured cells, in order to investigate their own angiogenesis potency in vivo. BM-aggregates and BM-MSC were seeded in Poly-L Lactic acid (PLLA) scaffold and implanted in the peritoneal cavity of mice.
Result:
Immunohistochemistry results indicated that there was a significant difference (p < 0.050) in CD31+ cells between PLLA scaffolds contained cultured BM-MSC; PLLA scaffolds contained BM-aggregates and empty PLLA. According to morphological evidence, obvious connections with recipient vasculature and acceptable integration with surroundings were established in MSC and aggregate-seeded scaffolds.
Conclusion:
Our findings revealed cultured BM-MSC and BM-aggregates, capacity in order to develop numerous connections between PLLA scaffold and recipient’s vasculature which is crucial to the survival of tissues, and considerable tendency to develop constructs containing CD31+ endothelial cells which can contribute in vessel’s tube formation.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>34250831</pmid><doi>10.1177/03913988211025538</doi><tpages>7</tpages></addata></record> |
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| subjects | Aggregates Angiogenesis Animals Bioengineering Blood vessels Bone Marrow Bone Marrow Cells Cell Differentiation Cells, Cultured Coculture Techniques Endothelial Cells Immunohistochemistry In vivo methods and tests Lactic acid Mesenchymal Stem Cells Mice Organs Peritoneum Polylactic acid Scaffolds Stem cells Tissue Engineering Tissue Scaffolds |
| title | In vivo study of the angiogenesis potential of bone marrow-derived mesenchymal stem cell aggregates in their niche like environment |
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