A natural product of acteoside ameliorate kidney injury in diabetes db/db mice and HK‐2 cells via regulating NADPH/oxidase‐TGF‐β/Smad signaling pathway
This study was designed to investigate the protective effects and mechanisms of acteoside on DKD in diabetes male db/db mice and high glucose‐induced HK‐2 cells. The diabetes db/db mice were divided randomly into model group, metformin group, irbesartan group, and acteoside group. We observed the na...
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| Veröffentlicht in: | Phytotherapy research 2021-09, Vol.35 (9), p.5227-5240 |
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| container_title | Phytotherapy research |
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| creator | Wang, Qinwen Dai, Xinxin Xiang, Xiang Xu, Zhuo Su, Shulan Wei, Dandan Zheng, Tianyao Shang, Er‐xin Qian, Dawei Duan, Jin‐ao |
| description | This study was designed to investigate the protective effects and mechanisms of acteoside on DKD in diabetes male db/db mice and high glucose‐induced HK‐2 cells. The diabetes db/db mice were divided randomly into model group, metformin group, irbesartan group, and acteoside group. We observed the natural product of acteoside exhibiting a significant effect in renal protection through analyzing of biochemical indicators and endogenous metabolites, histopathological observations, and western blotting. HK‐2 cells subjected to high glucose were used in invitro experiments. The molecular mechanisms of them were investigated by RT‐PCR and western blot. Acteoside prevents high glucose‐induced HK‐2 cells and diabetes db/db mice by inhibiting NADPH/oxidase‐TGF‐β/Smad signaling pathway. Acteoside regulated the disturbed metabolic pathway of lipid metabolism, glyoxylate and dicarboxylate metabolism, and arachidonic acid metabolism. We discovered the natural product of acteoside exhibiting a significant effect in renal protection. This study paved the way for further exploration of pathogenesis, early diagnosis, and development of a new therapeutic agent for DKD. |
| doi_str_mv | 10.1002/ptr.7196 |
| format | Article |
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The diabetes db/db mice were divided randomly into model group, metformin group, irbesartan group, and acteoside group. We observed the natural product of acteoside exhibiting a significant effect in renal protection through analyzing of biochemical indicators and endogenous metabolites, histopathological observations, and western blotting. HK‐2 cells subjected to high glucose were used in invitro experiments. The molecular mechanisms of them were investigated by RT‐PCR and western blot. Acteoside prevents high glucose‐induced HK‐2 cells and diabetes db/db mice by inhibiting NADPH/oxidase‐TGF‐β/Smad signaling pathway. Acteoside regulated the disturbed metabolic pathway of lipid metabolism, glyoxylate and dicarboxylate metabolism, and arachidonic acid metabolism. We discovered the natural product of acteoside exhibiting a significant effect in renal protection. This study paved the way for further exploration of pathogenesis, early diagnosis, and development of a new therapeutic agent for DKD.</description><identifier>ISSN: 0951-418X</identifier><identifier>EISSN: 1099-1573</identifier><identifier>DOI: 10.1002/ptr.7196</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>acteoside ; Arachidonic acid ; Chemical compounds ; Diabetes ; Diabetes mellitus ; diabetic kidney disease ; Glucose ; Kidneys ; Lipid metabolism ; Lipids ; Metabolic pathways ; metabolic profiling ; Metabolism ; Metabolites ; Metformin ; Molecular modelling ; NAD(P)H oxidase ; NADPH/oxidase‐TGF‐β/Smad signaling pathway ; Natural products ; Oxidase ; Pathogenesis ; Pharmacology ; ROS ; Signal transduction ; Signaling ; Smad protein ; Western blotting</subject><ispartof>Phytotherapy research, 2021-09, Vol.35 (9), p.5227-5240</ispartof><rights>2021 John Wiley & Sons Ltd.</rights><rights>2021 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3266-4165c3417010c64a3be89996348619cf97c5ab41015d93acf4bb337774cb8cb63</citedby><cites>FETCH-LOGICAL-c3266-4165c3417010c64a3be89996348619cf97c5ab41015d93acf4bb337774cb8cb63</cites><orcidid>0000-0002-2008-5887</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fptr.7196$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fptr.7196$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Wang, Qinwen</creatorcontrib><creatorcontrib>Dai, Xinxin</creatorcontrib><creatorcontrib>Xiang, Xiang</creatorcontrib><creatorcontrib>Xu, Zhuo</creatorcontrib><creatorcontrib>Su, Shulan</creatorcontrib><creatorcontrib>Wei, Dandan</creatorcontrib><creatorcontrib>Zheng, Tianyao</creatorcontrib><creatorcontrib>Shang, Er‐xin</creatorcontrib><creatorcontrib>Qian, Dawei</creatorcontrib><creatorcontrib>Duan, Jin‐ao</creatorcontrib><title>A natural product of acteoside ameliorate kidney injury in diabetes db/db mice and HK‐2 cells via regulating NADPH/oxidase‐TGF‐β/Smad signaling pathway</title><title>Phytotherapy research</title><description>This study was designed to investigate the protective effects and mechanisms of acteoside on DKD in diabetes male db/db mice and high glucose‐induced HK‐2 cells. The diabetes db/db mice were divided randomly into model group, metformin group, irbesartan group, and acteoside group. We observed the natural product of acteoside exhibiting a significant effect in renal protection through analyzing of biochemical indicators and endogenous metabolites, histopathological observations, and western blotting. HK‐2 cells subjected to high glucose were used in invitro experiments. The molecular mechanisms of them were investigated by RT‐PCR and western blot. Acteoside prevents high glucose‐induced HK‐2 cells and diabetes db/db mice by inhibiting NADPH/oxidase‐TGF‐β/Smad signaling pathway. Acteoside regulated the disturbed metabolic pathway of lipid metabolism, glyoxylate and dicarboxylate metabolism, and arachidonic acid metabolism. We discovered the natural product of acteoside exhibiting a significant effect in renal protection. This study paved the way for further exploration of pathogenesis, early diagnosis, and development of a new therapeutic agent for DKD.</description><subject>acteoside</subject><subject>Arachidonic acid</subject><subject>Chemical compounds</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>diabetic kidney disease</subject><subject>Glucose</subject><subject>Kidneys</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Metabolic pathways</subject><subject>metabolic profiling</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metformin</subject><subject>Molecular modelling</subject><subject>NAD(P)H oxidase</subject><subject>NADPH/oxidase‐TGF‐β/Smad signaling pathway</subject><subject>Natural products</subject><subject>Oxidase</subject><subject>Pathogenesis</subject><subject>Pharmacology</subject><subject>ROS</subject><subject>Signal transduction</subject><subject>Signaling</subject><subject>Smad protein</subject><subject>Western blotting</subject><issn>0951-418X</issn><issn>1099-1573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp10UtuFDEQBmALEYkhQeIIltiw6Yzddtvt5SiQDCIiURgkdq3yowcP_RhsN2F2HIETcAgOwiE4Ce4kKyQ2VZtPpar6EXpOySklpFzuUziVVIlHaEGJUgWtJHuMFkRVtOC0_vgEPY1xRwhRJeEL9HOFB0hTgA7vw2gnk_DYYjDJjdFbh6F3nR8DJIc_ezu4A_bDbgpzw9aDdslFbPXSatx7k_1g8frtn-8_Smxc10X81QMObjt1kPywxe9Wr67Xy_GbtxBdZpuL81x__1q-78Hi6LcDdLPbQ_p0C4cTdNRCF92zh36MPpy_3pyti8urizdnq8vCsFKIfJioDONUEkqM4MC0q5VSgvFaUGVaJU0FmlNCK6sYmJZrzZiUkhtdGy3YMXp5Pzc_4cvkYmp6H-cDYHDjFJuy4krUqpYy0xf_0N04hbz2rGR2VN2ph4EmjDEG1zb74HsIh4aSZg6qyUE1c1CZFvf01nfu8F_XXG9u7vxfarCYFA</recordid><startdate>202109</startdate><enddate>202109</enddate><creator>Wang, Qinwen</creator><creator>Dai, Xinxin</creator><creator>Xiang, Xiang</creator><creator>Xu, Zhuo</creator><creator>Su, Shulan</creator><creator>Wei, Dandan</creator><creator>Zheng, Tianyao</creator><creator>Shang, Er‐xin</creator><creator>Qian, Dawei</creator><creator>Duan, Jin‐ao</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2008-5887</orcidid></search><sort><creationdate>202109</creationdate><title>A natural product of acteoside ameliorate kidney injury in diabetes db/db mice and HK‐2 cells via regulating NADPH/oxidase‐TGF‐β/Smad signaling pathway</title><author>Wang, Qinwen ; Dai, Xinxin ; Xiang, Xiang ; Xu, Zhuo ; Su, Shulan ; Wei, Dandan ; Zheng, Tianyao ; Shang, Er‐xin ; Qian, Dawei ; Duan, Jin‐ao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3266-4165c3417010c64a3be89996348619cf97c5ab41015d93acf4bb337774cb8cb63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>acteoside</topic><topic>Arachidonic acid</topic><topic>Chemical compounds</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>diabetic kidney disease</topic><topic>Glucose</topic><topic>Kidneys</topic><topic>Lipid metabolism</topic><topic>Lipids</topic><topic>Metabolic pathways</topic><topic>metabolic profiling</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Metformin</topic><topic>Molecular modelling</topic><topic>NAD(P)H oxidase</topic><topic>NADPH/oxidase‐TGF‐β/Smad signaling pathway</topic><topic>Natural products</topic><topic>Oxidase</topic><topic>Pathogenesis</topic><topic>Pharmacology</topic><topic>ROS</topic><topic>Signal transduction</topic><topic>Signaling</topic><topic>Smad protein</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Qinwen</creatorcontrib><creatorcontrib>Dai, Xinxin</creatorcontrib><creatorcontrib>Xiang, Xiang</creatorcontrib><creatorcontrib>Xu, Zhuo</creatorcontrib><creatorcontrib>Su, Shulan</creatorcontrib><creatorcontrib>Wei, Dandan</creatorcontrib><creatorcontrib>Zheng, Tianyao</creatorcontrib><creatorcontrib>Shang, Er‐xin</creatorcontrib><creatorcontrib>Qian, Dawei</creatorcontrib><creatorcontrib>Duan, Jin‐ao</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytotherapy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Qinwen</au><au>Dai, Xinxin</au><au>Xiang, Xiang</au><au>Xu, Zhuo</au><au>Su, Shulan</au><au>Wei, Dandan</au><au>Zheng, Tianyao</au><au>Shang, Er‐xin</au><au>Qian, Dawei</au><au>Duan, Jin‐ao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A natural product of acteoside ameliorate kidney injury in diabetes db/db mice and HK‐2 cells via regulating NADPH/oxidase‐TGF‐β/Smad signaling pathway</atitle><jtitle>Phytotherapy research</jtitle><date>2021-09</date><risdate>2021</risdate><volume>35</volume><issue>9</issue><spage>5227</spage><epage>5240</epage><pages>5227-5240</pages><issn>0951-418X</issn><eissn>1099-1573</eissn><abstract>This study was designed to investigate the protective effects and mechanisms of acteoside on DKD in diabetes male db/db mice and high glucose‐induced HK‐2 cells. The diabetes db/db mice were divided randomly into model group, metformin group, irbesartan group, and acteoside group. We observed the natural product of acteoside exhibiting a significant effect in renal protection through analyzing of biochemical indicators and endogenous metabolites, histopathological observations, and western blotting. HK‐2 cells subjected to high glucose were used in invitro experiments. The molecular mechanisms of them were investigated by RT‐PCR and western blot. Acteoside prevents high glucose‐induced HK‐2 cells and diabetes db/db mice by inhibiting NADPH/oxidase‐TGF‐β/Smad signaling pathway. Acteoside regulated the disturbed metabolic pathway of lipid metabolism, glyoxylate and dicarboxylate metabolism, and arachidonic acid metabolism. We discovered the natural product of acteoside exhibiting a significant effect in renal protection. This study paved the way for further exploration of pathogenesis, early diagnosis, and development of a new therapeutic agent for DKD.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/ptr.7196</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-2008-5887</orcidid></addata></record> |
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| subjects | acteoside Arachidonic acid Chemical compounds Diabetes Diabetes mellitus diabetic kidney disease Glucose Kidneys Lipid metabolism Lipids Metabolic pathways metabolic profiling Metabolism Metabolites Metformin Molecular modelling NAD(P)H oxidase NADPH/oxidase‐TGF‐β/Smad signaling pathway Natural products Oxidase Pathogenesis Pharmacology ROS Signal transduction Signaling Smad protein Western blotting |
| title | A natural product of acteoside ameliorate kidney injury in diabetes db/db mice and HK‐2 cells via regulating NADPH/oxidase‐TGF‐β/Smad signaling pathway |
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