Single-cell transcriptomic analysis of endometriosis provides insights into fibroblast fates and immune cell heterogeneity
Background Endometriosis is an oestrogen-dependent disease with an unclear aetiology and pathogenesis affecting 6-10% of the global female population, predominantly those of reproductive age. Herein, we profile the transcriptomes of approximately 55,000 single cells from three groups including ectop...
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| Veröffentlicht in: | Cell & bioscience 2021-07, Vol.11 (1), p.1-125, Article 125 |
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| description | Background Endometriosis is an oestrogen-dependent disease with an unclear aetiology and pathogenesis affecting 6-10% of the global female population, predominantly those of reproductive age. Herein, we profile the transcriptomes of approximately 55,000 single cells from three groups including ectopic endometrium, eutopic endometrium from women with endometriosis, and eutopic endometrium from healthy women to create a single-cell transcriptome atlas of endometriosis. Results We have identified 9 cell types and performed single-cell analysis of fibroblasts, and determined a potential developmental trajectory associated with endometriosis. We also identified fibroblast subpopulations related to endometriosis development and found that StAR played an important role in this process. Moreover, T cells in endometriosis were less activated or inflammatory with decreased effector CD8 + T cells, while the composition ratio of natural killer cells decreased and the percentage of monocytes/macrophages increased in endometriosis cysts. In addition, the effectiveness of immune cells in endometriosis lesions, eutopic endometrium from women with endometriosis, and eutopic endometrium from healthy women was distinct. Cell-cell interaction analyses highlighted the imbalanced immune environment in endometriosis lesions and immune cells in endometriosis could promote the development of the disease. Conclusion Our study provided a systematic characterisation of endometriosis and insights into the aetiology and pathology of endometriosis. |
| doi_str_mv | 10.1186/s13578-021-00637-x |
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Herein, we profile the transcriptomes of approximately 55,000 single cells from three groups including ectopic endometrium, eutopic endometrium from women with endometriosis, and eutopic endometrium from healthy women to create a single-cell transcriptome atlas of endometriosis. Results We have identified 9 cell types and performed single-cell analysis of fibroblasts, and determined a potential developmental trajectory associated with endometriosis. We also identified fibroblast subpopulations related to endometriosis development and found that StAR played an important role in this process. Moreover, T cells in endometriosis were less activated or inflammatory with decreased effector CD8 + T cells, while the composition ratio of natural killer cells decreased and the percentage of monocytes/macrophages increased in endometriosis cysts. In addition, the effectiveness of immune cells in endometriosis lesions, eutopic endometrium from women with endometriosis, and eutopic endometrium from healthy women was distinct. Cell-cell interaction analyses highlighted the imbalanced immune environment in endometriosis lesions and immune cells in endometriosis could promote the development of the disease. Conclusion Our study provided a systematic characterisation of endometriosis and insights into the aetiology and pathology of endometriosis.</description><identifier>ISSN: 2045-3701</identifier><identifier>EISSN: 2045-3701</identifier><identifier>DOI: 10.1186/s13578-021-00637-x</identifier><identifier>PMID: 34233737</identifier><language>eng</language><publisher>LONDON: Springer Nature</publisher><subject>Analysis ; Angiogenesis ; Biochemistry & Molecular Biology ; CD8 antigen ; Cysts ; Cytokines ; Development and progression ; Effector cells ; Endometriosis ; Endometrium ; Estrogen ; Estrogens ; Fibroblast ; Fibroblasts ; Gene expression ; Genomics ; Health aspects ; Immune cell heterogeneity ; Inflammation ; Killer cells ; Life Sciences & Biomedicine ; Lymphocytes T ; Macrophages ; Metabolism ; Monocytes ; Morphogenesis ; Motility ; Natural killer cells ; Pathogenesis ; Population ; Science & Technology ; Single-cell sequence ; T cells ; Transcriptomes ; Women ; Womens health</subject><ispartof>Cell & bioscience, 2021-07, Vol.11 (1), p.1-125, Article 125</ispartof><rights>COPYRIGHT 2021 BioMed Central Ltd.</rights><rights>2021. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>46</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000674317000001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c574t-ff37542d93a42bf3c0ec8924008416bfff893fa828654bad6807d5af8c0f00023</citedby><cites>FETCH-LOGICAL-c574t-ff37542d93a42bf3c0ec8924008416bfff893fa828654bad6807d5af8c0f00023</cites><orcidid>0000-0002-1370-1872</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261960/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261960/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2104,2116,27931,27932,39265,53798,53800</link.rule.ids></links><search><creatorcontrib>Ma, Junyan</creatorcontrib><creatorcontrib>Zhang, Liqi</creatorcontrib><creatorcontrib>Zhan, Hong</creatorcontrib><creatorcontrib>Mo, Yun</creatorcontrib><creatorcontrib>Ren, Zuanjie</creatorcontrib><creatorcontrib>Shao, Anwen</creatorcontrib><creatorcontrib>Lin, Jun</creatorcontrib><title>Single-cell transcriptomic analysis of endometriosis provides insights into fibroblast fates and immune cell heterogeneity</title><title>Cell & bioscience</title><addtitle>CELL BIOSCI</addtitle><description>Background Endometriosis is an oestrogen-dependent disease with an unclear aetiology and pathogenesis affecting 6-10% of the global female population, predominantly those of reproductive age. Herein, we profile the transcriptomes of approximately 55,000 single cells from three groups including ectopic endometrium, eutopic endometrium from women with endometriosis, and eutopic endometrium from healthy women to create a single-cell transcriptome atlas of endometriosis. Results We have identified 9 cell types and performed single-cell analysis of fibroblasts, and determined a potential developmental trajectory associated with endometriosis. We also identified fibroblast subpopulations related to endometriosis development and found that StAR played an important role in this process. Moreover, T cells in endometriosis were less activated or inflammatory with decreased effector CD8 + T cells, while the composition ratio of natural killer cells decreased and the percentage of monocytes/macrophages increased in endometriosis cysts. In addition, the effectiveness of immune cells in endometriosis lesions, eutopic endometrium from women with endometriosis, and eutopic endometrium from healthy women was distinct. Cell-cell interaction analyses highlighted the imbalanced immune environment in endometriosis lesions and immune cells in endometriosis could promote the development of the disease. Conclusion Our study provided a systematic characterisation of endometriosis and insights into the aetiology and pathology of endometriosis.</description><subject>Analysis</subject><subject>Angiogenesis</subject><subject>Biochemistry & Molecular Biology</subject><subject>CD8 antigen</subject><subject>Cysts</subject><subject>Cytokines</subject><subject>Development and progression</subject><subject>Effector cells</subject><subject>Endometriosis</subject><subject>Endometrium</subject><subject>Estrogen</subject><subject>Estrogens</subject><subject>Fibroblast</subject><subject>Fibroblasts</subject><subject>Gene expression</subject><subject>Genomics</subject><subject>Health aspects</subject><subject>Immune cell heterogeneity</subject><subject>Inflammation</subject><subject>Killer cells</subject><subject>Life Sciences & Biomedicine</subject><subject>Lymphocytes T</subject><subject>Macrophages</subject><subject>Metabolism</subject><subject>Monocytes</subject><subject>Morphogenesis</subject><subject>Motility</subject><subject>Natural killer cells</subject><subject>Pathogenesis</subject><subject>Population</subject><subject>Science & Technology</subject><subject>Single-cell sequence</subject><subject>T cells</subject><subject>Transcriptomes</subject><subject>Women</subject><subject>Womens health</subject><issn>2045-3701</issn><issn>2045-3701</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkktv1DAUhSMEolXpH2AViQ0IpfiV2NkgVSMelSohUVhbjnOd8SixB9tpZ_j1OJ2qMIgF8SLW9XeO5XtPUbzE6AJj0byLmNZcVIjgCqGG8mr3pDgliNUV5Qg__WN_UpzHuEH5Yy1GvH5enFBGKOWUnxY_b6wbRqg0jGOZgnJRB7tNfrK6VE6N-2hj6U0JrvcTpGD9UtgGf2t7iKV10Q7rtGySL43tgu9GFVNpVMrHyvWlnabZQXl_wRoSBD-AA5v2L4pnRo0Rzh_-Z8X3jx--rT5X118-Xa0urytdc5YqYyivGelbqhjpDNUItGgJQ0gw3HTGGNFSowQRTc061TcC8b5WRmhk8psJPSuuDr69Vxu5DXZSYS-9svK-4MMgVUhWjyA5U0grjIFhwnD2AC5Ao7rtO9piQbPX-4PXdu4m6DW43LPxyPT4xNm1HPytFKTBbYOywesHg-B_zBCTnGxceqMc-DlKUrO2EbylIqOv_kI3fg55JgtVU5rny-vf1KDyA6wzPt-rF1N52XBCCSGcZ-riH1RePeRJewfG5vqR4M2RIDMJdmlQc4zy6ubrMUsOrA4-xgDmsR8YySWs8hBWmcMq78Mqd1n09iC6g86bqC04DY_CPLiGM4r5EluEMy3-n17ZpJL1buVnl-gvMJX8Hg</recordid><startdate>20210707</startdate><enddate>20210707</enddate><creator>Ma, Junyan</creator><creator>Zhang, Liqi</creator><creator>Zhan, Hong</creator><creator>Mo, Yun</creator><creator>Ren, Zuanjie</creator><creator>Shao, Anwen</creator><creator>Lin, Jun</creator><general>Springer Nature</general><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-1370-1872</orcidid></search><sort><creationdate>20210707</creationdate><title>Single-cell transcriptomic analysis of endometriosis provides insights into fibroblast fates and immune cell heterogeneity</title><author>Ma, Junyan ; 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Herein, we profile the transcriptomes of approximately 55,000 single cells from three groups including ectopic endometrium, eutopic endometrium from women with endometriosis, and eutopic endometrium from healthy women to create a single-cell transcriptome atlas of endometriosis. Results We have identified 9 cell types and performed single-cell analysis of fibroblasts, and determined a potential developmental trajectory associated with endometriosis. We also identified fibroblast subpopulations related to endometriosis development and found that StAR played an important role in this process. Moreover, T cells in endometriosis were less activated or inflammatory with decreased effector CD8 + T cells, while the composition ratio of natural killer cells decreased and the percentage of monocytes/macrophages increased in endometriosis cysts. In addition, the effectiveness of immune cells in endometriosis lesions, eutopic endometrium from women with endometriosis, and eutopic endometrium from healthy women was distinct. Cell-cell interaction analyses highlighted the imbalanced immune environment in endometriosis lesions and immune cells in endometriosis could promote the development of the disease. Conclusion Our study provided a systematic characterisation of endometriosis and insights into the aetiology and pathology of endometriosis.</abstract><cop>LONDON</cop><pub>Springer Nature</pub><pmid>34233737</pmid><doi>10.1186/s13578-021-00637-x</doi><tpages>19</tpages><orcidid>https://orcid.org/0000-0002-1370-1872</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Angiogenesis Biochemistry & Molecular Biology CD8 antigen Cysts Cytokines Development and progression Effector cells Endometriosis Endometrium Estrogen Estrogens Fibroblast Fibroblasts Gene expression Genomics Health aspects Immune cell heterogeneity Inflammation Killer cells Life Sciences & Biomedicine Lymphocytes T Macrophages Metabolism Monocytes Morphogenesis Motility Natural killer cells Pathogenesis Population Science & Technology Single-cell sequence T cells Transcriptomes Women Womens health |
| title | Single-cell transcriptomic analysis of endometriosis provides insights into fibroblast fates and immune cell heterogeneity |
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