Vitrification of mouse two‐cell and blastocyst stage embryos in simplified closed system using either a hemi‐straw or a hollow fiber device
Two‐cell stage and blastocyst stage mouse embryos were equilibrated in a medium containing 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO) for 8–15 min. Vitrification was performed in a medium containing 0.5 M sucrose and either 15% EG + 15% DMSO, 17.5% EG + 17.5% DMSO, or 20% EG + 20%...
Gespeichert in:
| Veröffentlicht in: | Animal science journal 2021-01, Vol.92 (1), p.e13585-n/a |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | n/a |
|---|---|
| container_issue | 1 |
| container_start_page | e13585 |
| container_title | Animal science journal |
| container_volume | 92 |
| creator | Suttirojpattana, Tayita Juanpanich, Theesit Parnpai, Rangsun Vutyavanich, Teraporn |
| description | Two‐cell stage and blastocyst stage mouse embryos were equilibrated in a medium containing 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO) for 8–15 min. Vitrification was performed in a medium containing 0.5 M sucrose and either 15% EG + 15% DMSO, 17.5% EG + 17.5% DMSO, or 20% EG + 20% DMSO for 30 s. They were then placed either on a hemi‐straw (HS) or a hollow fiber vitrification (HFV) device and vitrified by cooled air inside a 0.5‐ml straw. In two‐cell embryos, a 100% survival rate was obtained from all groups except the 20% HS group (P > .05). All vitrified two‐cell groups showed similar rates of blastocyst development to that of fresh control group (P > .05), except 17.5% and 20% HFV groups, which were significantly lower than the other groups (P |
| doi_str_mv | 10.1111/asj.13585 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2549201383</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2799033296</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3375-691a767f27de228696d8a6ed79c8b315d08b2007a96bcba3fd64db441b69f9fc3</originalsourceid><addsrcrecordid>eNp1kctu1DAUhi0EoqWw4AWQJTawSOtL4sTLquKqSiy4bCNfjluPnHjwSRjNjjeAZ-RJcDuFBRJn8x9Znz7Z_gl5ytkpr3NmcHPKZTd098gx71vWMC30_brLtm2kbvkReYS4YYz3mnUPyZFsheS6E8fkx5e4lBiiM0vMM82BTnlFoMsu__r-00FK1Mye2mRwyW6PC8XFXAGFyZZ9RhpninHapqoAT13KWAMrBxNdMc5XFOJyDYUaeg1TrE5citnRfHuSU8o7GqKtgIdv0cFj8iCYhPDkLk_I59evPl28bS4_vHl3cX7ZOCn7rlGam171QfQehBiUVn4wCnyv3WAl7zwbrGCsN1pZZ40MXrXeti23SgcdnDwhLw7ebclfV8BlnCLePNfMUD9gFF2rBeNykBV9_g-6yWuZ6-1G0WvNpBRaVerlgXIlIxYI47bEyZT9yNl409JYWxpvW6rsszvjaifwf8k_tVTg7ADsYoL9_03j-cf3B-VvaZ6frw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2799033296</pqid></control><display><type>article</type><title>Vitrification of mouse two‐cell and blastocyst stage embryos in simplified closed system using either a hemi‐straw or a hollow fiber device</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Alma/SFX Local Collection</source><creator>Suttirojpattana, Tayita ; Juanpanich, Theesit ; Parnpai, Rangsun ; Vutyavanich, Teraporn</creator><creatorcontrib>Suttirojpattana, Tayita ; Juanpanich, Theesit ; Parnpai, Rangsun ; Vutyavanich, Teraporn</creatorcontrib><description>Two‐cell stage and blastocyst stage mouse embryos were equilibrated in a medium containing 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO) for 8–15 min. Vitrification was performed in a medium containing 0.5 M sucrose and either 15% EG + 15% DMSO, 17.5% EG + 17.5% DMSO, or 20% EG + 20% DMSO for 30 s. They were then placed either on a hemi‐straw (HS) or a hollow fiber vitrification (HFV) device and vitrified by cooled air inside a 0.5‐ml straw. In two‐cell embryos, a 100% survival rate was obtained from all groups except the 20% HS group (P > .05). All vitrified two‐cell groups showed similar rates of blastocyst development to that of fresh control group (P > .05), except 17.5% and 20% HFV groups, which were significantly lower than the other groups (P < .05). In the blastocyst embryos, the HFV groups were divided into two subgroups (non‐collapsed; HFV‐NC and collapsed; HFV‐C blastocyst). Re‐expansion rate in 15% HFV‐NC, 17.5% HFV‐NC, and 15% HFV‐C groups was reduced (P < .05), whereas the rest were similar to control. In conclusion, we established a simplified, reliable, and closed system for HFV vitrification applying hemi‐straw, which does not require skilled practitioners.</description><identifier>ISSN: 1344-3941</identifier><identifier>EISSN: 1740-0929</identifier><identifier>DOI: 10.1111/asj.13585</identifier><identifier>PMID: 34231952</identifier><language>eng</language><publisher>Australia: Blackwell Publishing Ltd</publisher><subject>Animals ; Blastocyst ; Cell survival ; closed vitrification system ; collapsed blastocyst ; Cryopreservation - veterinary ; Cryoprotective Agents ; Dimethyl Sulfoxide ; Embryos ; Ethylene Glycol ; hollow fiber vitrification ; Mice ; mouse embryo ; Straw ; Subgroups ; Sucrose ; Survival ; Vitrification</subject><ispartof>Animal science journal, 2021-01, Vol.92 (1), p.e13585-n/a</ispartof><rights>2021 Japanese Society of Animal Science</rights><rights>2021 Japanese Society of Animal Science.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3375-691a767f27de228696d8a6ed79c8b315d08b2007a96bcba3fd64db441b69f9fc3</cites><orcidid>0000-0001-6750-3137 ; 0000-0001-5901-3277 ; 0000-0003-4377-6645</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fasj.13585$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fasj.13585$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34231952$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suttirojpattana, Tayita</creatorcontrib><creatorcontrib>Juanpanich, Theesit</creatorcontrib><creatorcontrib>Parnpai, Rangsun</creatorcontrib><creatorcontrib>Vutyavanich, Teraporn</creatorcontrib><title>Vitrification of mouse two‐cell and blastocyst stage embryos in simplified closed system using either a hemi‐straw or a hollow fiber device</title><title>Animal science journal</title><addtitle>Anim Sci J</addtitle><description>Two‐cell stage and blastocyst stage mouse embryos were equilibrated in a medium containing 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO) for 8–15 min. Vitrification was performed in a medium containing 0.5 M sucrose and either 15% EG + 15% DMSO, 17.5% EG + 17.5% DMSO, or 20% EG + 20% DMSO for 30 s. They were then placed either on a hemi‐straw (HS) or a hollow fiber vitrification (HFV) device and vitrified by cooled air inside a 0.5‐ml straw. In two‐cell embryos, a 100% survival rate was obtained from all groups except the 20% HS group (P > .05). All vitrified two‐cell groups showed similar rates of blastocyst development to that of fresh control group (P > .05), except 17.5% and 20% HFV groups, which were significantly lower than the other groups (P < .05). In the blastocyst embryos, the HFV groups were divided into two subgroups (non‐collapsed; HFV‐NC and collapsed; HFV‐C blastocyst). Re‐expansion rate in 15% HFV‐NC, 17.5% HFV‐NC, and 15% HFV‐C groups was reduced (P < .05), whereas the rest were similar to control. In conclusion, we established a simplified, reliable, and closed system for HFV vitrification applying hemi‐straw, which does not require skilled practitioners.</description><subject>Animals</subject><subject>Blastocyst</subject><subject>Cell survival</subject><subject>closed vitrification system</subject><subject>collapsed blastocyst</subject><subject>Cryopreservation - veterinary</subject><subject>Cryoprotective Agents</subject><subject>Dimethyl Sulfoxide</subject><subject>Embryos</subject><subject>Ethylene Glycol</subject><subject>hollow fiber vitrification</subject><subject>Mice</subject><subject>mouse embryo</subject><subject>Straw</subject><subject>Subgroups</subject><subject>Sucrose</subject><subject>Survival</subject><subject>Vitrification</subject><issn>1344-3941</issn><issn>1740-0929</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctu1DAUhi0EoqWw4AWQJTawSOtL4sTLquKqSiy4bCNfjluPnHjwSRjNjjeAZ-RJcDuFBRJn8x9Znz7Z_gl5ytkpr3NmcHPKZTd098gx71vWMC30_brLtm2kbvkReYS4YYz3mnUPyZFsheS6E8fkx5e4lBiiM0vMM82BTnlFoMsu__r-00FK1Mye2mRwyW6PC8XFXAGFyZZ9RhpninHapqoAT13KWAMrBxNdMc5XFOJyDYUaeg1TrE5citnRfHuSU8o7GqKtgIdv0cFj8iCYhPDkLk_I59evPl28bS4_vHl3cX7ZOCn7rlGam171QfQehBiUVn4wCnyv3WAl7zwbrGCsN1pZZ40MXrXeti23SgcdnDwhLw7ebclfV8BlnCLePNfMUD9gFF2rBeNykBV9_g-6yWuZ6-1G0WvNpBRaVerlgXIlIxYI47bEyZT9yNl409JYWxpvW6rsszvjaifwf8k_tVTg7ADsYoL9_03j-cf3B-VvaZ6frw</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Suttirojpattana, Tayita</creator><creator>Juanpanich, Theesit</creator><creator>Parnpai, Rangsun</creator><creator>Vutyavanich, Teraporn</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6750-3137</orcidid><orcidid>https://orcid.org/0000-0001-5901-3277</orcidid><orcidid>https://orcid.org/0000-0003-4377-6645</orcidid></search><sort><creationdate>202101</creationdate><title>Vitrification of mouse two‐cell and blastocyst stage embryos in simplified closed system using either a hemi‐straw or a hollow fiber device</title><author>Suttirojpattana, Tayita ; Juanpanich, Theesit ; Parnpai, Rangsun ; Vutyavanich, Teraporn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3375-691a767f27de228696d8a6ed79c8b315d08b2007a96bcba3fd64db441b69f9fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Blastocyst</topic><topic>Cell survival</topic><topic>closed vitrification system</topic><topic>collapsed blastocyst</topic><topic>Cryopreservation - veterinary</topic><topic>Cryoprotective Agents</topic><topic>Dimethyl Sulfoxide</topic><topic>Embryos</topic><topic>Ethylene Glycol</topic><topic>hollow fiber vitrification</topic><topic>Mice</topic><topic>mouse embryo</topic><topic>Straw</topic><topic>Subgroups</topic><topic>Sucrose</topic><topic>Survival</topic><topic>Vitrification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suttirojpattana, Tayita</creatorcontrib><creatorcontrib>Juanpanich, Theesit</creatorcontrib><creatorcontrib>Parnpai, Rangsun</creatorcontrib><creatorcontrib>Vutyavanich, Teraporn</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Animal science journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suttirojpattana, Tayita</au><au>Juanpanich, Theesit</au><au>Parnpai, Rangsun</au><au>Vutyavanich, Teraporn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vitrification of mouse two‐cell and blastocyst stage embryos in simplified closed system using either a hemi‐straw or a hollow fiber device</atitle><jtitle>Animal science journal</jtitle><addtitle>Anim Sci J</addtitle><date>2021-01</date><risdate>2021</risdate><volume>92</volume><issue>1</issue><spage>e13585</spage><epage>n/a</epage><pages>e13585-n/a</pages><issn>1344-3941</issn><eissn>1740-0929</eissn><abstract>Two‐cell stage and blastocyst stage mouse embryos were equilibrated in a medium containing 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO) for 8–15 min. Vitrification was performed in a medium containing 0.5 M sucrose and either 15% EG + 15% DMSO, 17.5% EG + 17.5% DMSO, or 20% EG + 20% DMSO for 30 s. They were then placed either on a hemi‐straw (HS) or a hollow fiber vitrification (HFV) device and vitrified by cooled air inside a 0.5‐ml straw. In two‐cell embryos, a 100% survival rate was obtained from all groups except the 20% HS group (P > .05). All vitrified two‐cell groups showed similar rates of blastocyst development to that of fresh control group (P > .05), except 17.5% and 20% HFV groups, which were significantly lower than the other groups (P < .05). In the blastocyst embryos, the HFV groups were divided into two subgroups (non‐collapsed; HFV‐NC and collapsed; HFV‐C blastocyst). Re‐expansion rate in 15% HFV‐NC, 17.5% HFV‐NC, and 15% HFV‐C groups was reduced (P < .05), whereas the rest were similar to control. In conclusion, we established a simplified, reliable, and closed system for HFV vitrification applying hemi‐straw, which does not require skilled practitioners.</abstract><cop>Australia</cop><pub>Blackwell Publishing Ltd</pub><pmid>34231952</pmid><doi>10.1111/asj.13585</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-6750-3137</orcidid><orcidid>https://orcid.org/0000-0001-5901-3277</orcidid><orcidid>https://orcid.org/0000-0003-4377-6645</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1344-3941 |
| ispartof | Animal science journal, 2021-01, Vol.92 (1), p.e13585-n/a |
| issn | 1344-3941 1740-0929 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2549201383 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Animals Blastocyst Cell survival closed vitrification system collapsed blastocyst Cryopreservation - veterinary Cryoprotective Agents Dimethyl Sulfoxide Embryos Ethylene Glycol hollow fiber vitrification Mice mouse embryo Straw Subgroups Sucrose Survival Vitrification |
| title | Vitrification of mouse two‐cell and blastocyst stage embryos in simplified closed system using either a hemi‐straw or a hollow fiber device |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T23%3A14%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Vitrification%20of%20mouse%20two%E2%80%90cell%20and%20blastocyst%20stage%20embryos%20in%20simplified%20closed%20system%20using%20either%20a%20hemi%E2%80%90straw%20or%20a%20hollow%20fiber%20device&rft.jtitle=Animal%20science%20journal&rft.au=Suttirojpattana,%20Tayita&rft.date=2021-01&rft.volume=92&rft.issue=1&rft.spage=e13585&rft.epage=n/a&rft.pages=e13585-n/a&rft.issn=1344-3941&rft.eissn=1740-0929&rft_id=info:doi/10.1111/asj.13585&rft_dat=%3Cproquest_cross%3E2799033296%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2799033296&rft_id=info:pmid/34231952&rfr_iscdi=true |