Serum antibodies blocked by glycan antigens in canine visceral leishmaniasis serology are mostly IgA immune complexes
Immune complexes (ICs) are found in canine visceral leishmaniasis (CVL) and interfere with the serum detection of antibodies. Dissociation of these monovalent complexes by dissociative enzyme-linked immunosorbent assay (ELISA) removes false-negative results and allows some characterization of antibo...
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| Veröffentlicht in: | Parasitology 2021-10, Vol.148 (12), p.1509-1515 |
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| creator | Aparecida de Carvalho, Camila Mitsuyoshi Hiramoto, Roberto Regina Meireles, Luciana Franco de Andrade Júnior, Heitor |
| description | Immune complexes (ICs) are found in canine visceral leishmaniasis (CVL) and interfere with the serum detection of antibodies. Dissociation of these monovalent complexes by dissociative enzyme-linked immunosorbent assay (ELISA) removes false-negative results and allows some characterization of antibodies and antigens. We studied the serology of dogs with suspected CVL in an endemic area, testing two Leishmania (Leishmania) [L. (L.)] infantum antigens. We analysed the presence of immunoglobulin G (IgG), immunoglobulin A (IgA) and immunoglobulin M (IgM) antibodies specific to promastigote soluble extract (PSE) and low-molecular weight glycans (glycan–bovine serum albumin (BSA) complex – GBC) by conventional and dissociative ELISA. Our results showed a significant fraction of IgA ICs (46.5% for PSE and 47.6% for GBC), followed by IgG ICs (10% for PSE and 23.5% for GBC). IgM ICs were more frequent for PSE (22.7%). Hypergammaglobulinaemia in CVL would be related to the presence of IgA and IgG ICs, resulting in deficient elimination of these antibodies. Our data confirmed the presence of ICs that can generate false-negative results in conventional serology. The production of IgA antibodies and the high frequency of blockade by glycan antigens suggest the active participation of this immunoglobulin and its ICs in the immunopathology of CVL, indicating a new path for further research. |
| doi_str_mv | 10.1017/S0031182021001189 |
| format | Article |
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Dissociation of these monovalent complexes by dissociative enzyme-linked immunosorbent assay (ELISA) removes false-negative results and allows some characterization of antibodies and antigens. We studied the serology of dogs with suspected CVL in an endemic area, testing two Leishmania (Leishmania) [L. (L.)] <full form>infantum antigens. We analysed the presence of immunoglobulin G (IgG), immunoglobulin A (IgA) and immunoglobulin M (IgM) antibodies specific to promastigote soluble extract (PSE) and low-molecular weight glycans (glycan–bovine serum albumin (BSA) complex – GBC) by conventional and dissociative ELISA. Our results showed a significant fraction of IgA ICs (46.5% for PSE and 47.6% for GBC), followed by IgG ICs (10% for PSE and 23.5% for GBC). IgM ICs were more frequent for PSE (22.7%). Hypergammaglobulinaemia in CVL would be related to the presence of IgA and IgG ICs, resulting in deficient elimination of these antibodies. Our data confirmed the presence of ICs that can generate false-negative results in conventional serology. The production of IgA antibodies and the high frequency of blockade by glycan antigens suggest the active participation of this immunoglobulin and its ICs in the immunopathology of CVL, indicating a new path for further research.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182021001189</identifier><identifier>PMID: 34218828</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Antibodies ; Antigen-Antibody Complex ; Antigen-antibody complexes ; Antigens ; Bovine serum albumin ; Cytokines ; Dog Diseases - diagnosis ; Dogs ; Enzyme-linked immunosorbent assay ; Glycan ; IgG antibody ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulin M ; Immunoglobulins ; Infections ; Leishmania ; Leishmaniasis, Visceral - diagnosis ; Leishmaniasis, Visceral - veterinary ; Molecular weight ; Parasitic diseases ; Polysaccharides ; Proteins ; Serology ; Serum albumin ; Tumor necrosis factor-TNF ; Variance analysis ; Vector-borne diseases ; Visceral leishmaniasis</subject><ispartof>Parasitology, 2021-10, Vol.148 (12), p.1509-1515</ispartof><rights>Copyright © The Author(s), 2021. Published by Cambridge University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-2f37468971b0b6a0f1e393003d0bccb3524b7c71a9342dc96ea648c706313fb43</citedby><cites>FETCH-LOGICAL-c443t-2f37468971b0b6a0f1e393003d0bccb3524b7c71a9342dc96ea648c706313fb43</cites><orcidid>0000-0003-1762-2749 ; 0000-0003-4697-4647</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182021001189/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,27923,27924,55627</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34218828$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aparecida de Carvalho, Camila</creatorcontrib><creatorcontrib>Mitsuyoshi Hiramoto, Roberto</creatorcontrib><creatorcontrib>Regina Meireles, Luciana</creatorcontrib><creatorcontrib>Franco de Andrade Júnior, Heitor</creatorcontrib><title>Serum antibodies blocked by glycan antigens in canine visceral leishmaniasis serology are mostly IgA immune complexes</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>Immune complexes (ICs) are found in canine visceral leishmaniasis (CVL) and interfere with the serum detection of antibodies. Dissociation of these monovalent complexes by dissociative enzyme-linked immunosorbent assay (ELISA) removes false-negative results and allows some characterization of antibodies and antigens. We studied the serology of dogs with suspected CVL in an endemic area, testing two Leishmania (Leishmania) [L. (L.)] <full form>infantum antigens. We analysed the presence of immunoglobulin G (IgG), immunoglobulin A (IgA) and immunoglobulin M (IgM) antibodies specific to promastigote soluble extract (PSE) and low-molecular weight glycans (glycan–bovine serum albumin (BSA) complex – GBC) by conventional and dissociative ELISA. Our results showed a significant fraction of IgA ICs (46.5% for PSE and 47.6% for GBC), followed by IgG ICs (10% for PSE and 23.5% for GBC). IgM ICs were more frequent for PSE (22.7%). Hypergammaglobulinaemia in CVL would be related to the presence of IgA and IgG ICs, resulting in deficient elimination of these antibodies. Our data confirmed the presence of ICs that can generate false-negative results in conventional serology. The production of IgA antibodies and the high frequency of blockade by glycan antigens suggest the active participation of this immunoglobulin and its ICs in the immunopathology of CVL, indicating a new path for further research.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antigen-Antibody Complex</subject><subject>Antigen-antibody complexes</subject><subject>Antigens</subject><subject>Bovine serum albumin</subject><subject>Cytokines</subject><subject>Dog Diseases - diagnosis</subject><subject>Dogs</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Glycan</subject><subject>IgG antibody</subject><subject>Immunoglobulin A</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin M</subject><subject>Immunoglobulins</subject><subject>Infections</subject><subject>Leishmania</subject><subject>Leishmaniasis, Visceral - diagnosis</subject><subject>Leishmaniasis, Visceral - veterinary</subject><subject>Molecular weight</subject><subject>Parasitic diseases</subject><subject>Polysaccharides</subject><subject>Proteins</subject><subject>Serology</subject><subject>Serum albumin</subject><subject>Tumor necrosis factor-TNF</subject><subject>Variance analysis</subject><subject>Vector-borne diseases</subject><subject>Visceral leishmaniasis</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1UctO3DAUtaqiMgU-oJvKUjfdpPiFYy8RohQJiQXtOrKdm2Bqx4M9QeTv64EplYpY-XEe9x4dhD5R8o0S2h7fEMIpVYwwSki96HdoRYXUjaKSvkerLdxs8X30sZQ7Qojkkn1A-1wwqhRTKzTfQJ4jNtPG29R7KNiG5H5Dj-2Cx7A4Mz2BI0wF-wnXt58AP_jiIJuAA_hyG-unKb7gAjmFNC7YZMAxlU1Y8OV4in2Mc1W5FNcBHqEcor3BhAJHu_MA_fp-_vPsR3N1fXF5dnrVOCH4pmEDb4VUuqWWWGnIQIFrXlP1xDpn-QkTtnUtNboG6p2WYKRQrq0xKR-s4Afo67PvOqf7Gcqmi9vFQzATpLl07EQoSZiivFK__Ee9S3Oe6naVJbXSWkhZWfSZ5XIqJcPQrbOPJi8dJd22k-5VJ1Xzeec82wj9i-JvCZXAd6Ym2uz7Ef7Nftv2D5e2lhE</recordid><startdate>20211001</startdate><enddate>20211001</enddate><creator>Aparecida de Carvalho, Camila</creator><creator>Mitsuyoshi Hiramoto, Roberto</creator><creator>Regina Meireles, Luciana</creator><creator>Franco de Andrade Júnior, Heitor</creator><general>Cambridge University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1762-2749</orcidid><orcidid>https://orcid.org/0000-0003-4697-4647</orcidid></search><sort><creationdate>20211001</creationdate><title>Serum antibodies blocked by glycan antigens in canine visceral leishmaniasis serology are mostly IgA immune complexes</title><author>Aparecida de Carvalho, Camila ; Mitsuyoshi Hiramoto, Roberto ; Regina Meireles, Luciana ; Franco de Andrade Júnior, Heitor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-2f37468971b0b6a0f1e393003d0bccb3524b7c71a9342dc96ea648c706313fb43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antigen-Antibody Complex</topic><topic>Antigen-antibody complexes</topic><topic>Antigens</topic><topic>Bovine serum albumin</topic><topic>Cytokines</topic><topic>Dog Diseases - diagnosis</topic><topic>Dogs</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Glycan</topic><topic>IgG antibody</topic><topic>Immunoglobulin A</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin M</topic><topic>Immunoglobulins</topic><topic>Infections</topic><topic>Leishmania</topic><topic>Leishmaniasis, Visceral - diagnosis</topic><topic>Leishmaniasis, Visceral - veterinary</topic><topic>Molecular weight</topic><topic>Parasitic diseases</topic><topic>Polysaccharides</topic><topic>Proteins</topic><topic>Serology</topic><topic>Serum albumin</topic><topic>Tumor necrosis factor-TNF</topic><topic>Variance analysis</topic><topic>Vector-borne diseases</topic><topic>Visceral leishmaniasis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aparecida de Carvalho, Camila</creatorcontrib><creatorcontrib>Mitsuyoshi Hiramoto, Roberto</creatorcontrib><creatorcontrib>Regina Meireles, Luciana</creatorcontrib><creatorcontrib>Franco de Andrade Júnior, Heitor</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aparecida de Carvalho, Camila</au><au>Mitsuyoshi Hiramoto, Roberto</au><au>Regina Meireles, Luciana</au><au>Franco de Andrade Júnior, Heitor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serum antibodies blocked by glycan antigens in canine visceral leishmaniasis serology are mostly IgA immune complexes</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2021-10-01</date><risdate>2021</risdate><volume>148</volume><issue>12</issue><spage>1509</spage><epage>1515</epage><pages>1509-1515</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><abstract>Immune complexes (ICs) are found in canine visceral leishmaniasis (CVL) and interfere with the serum detection of antibodies. Dissociation of these monovalent complexes by dissociative enzyme-linked immunosorbent assay (ELISA) removes false-negative results and allows some characterization of antibodies and antigens. We studied the serology of dogs with suspected CVL in an endemic area, testing two Leishmania (Leishmania) [L. (L.)] <full form>infantum antigens. We analysed the presence of immunoglobulin G (IgG), immunoglobulin A (IgA) and immunoglobulin M (IgM) antibodies specific to promastigote soluble extract (PSE) and low-molecular weight glycans (glycan–bovine serum albumin (BSA) complex – GBC) by conventional and dissociative ELISA. Our results showed a significant fraction of IgA ICs (46.5% for PSE and 47.6% for GBC), followed by IgG ICs (10% for PSE and 23.5% for GBC). IgM ICs were more frequent for PSE (22.7%). Hypergammaglobulinaemia in CVL would be related to the presence of IgA and IgG ICs, resulting in deficient elimination of these antibodies. Our data confirmed the presence of ICs that can generate false-negative results in conventional serology. The production of IgA antibodies and the high frequency of blockade by glycan antigens suggest the active participation of this immunoglobulin and its ICs in the immunopathology of CVL, indicating a new path for further research.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>34218828</pmid><doi>10.1017/S0031182021001189</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-1762-2749</orcidid><orcidid>https://orcid.org/0000-0003-4697-4647</orcidid></addata></record> |
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| subjects | Animals Antibodies Antigen-Antibody Complex Antigen-antibody complexes Antigens Bovine serum albumin Cytokines Dog Diseases - diagnosis Dogs Enzyme-linked immunosorbent assay Glycan IgG antibody Immunoglobulin A Immunoglobulin G Immunoglobulin M Immunoglobulins Infections Leishmania Leishmaniasis, Visceral - diagnosis Leishmaniasis, Visceral - veterinary Molecular weight Parasitic diseases Polysaccharides Proteins Serology Serum albumin Tumor necrosis factor-TNF Variance analysis Vector-borne diseases Visceral leishmaniasis |
| title | Serum antibodies blocked by glycan antigens in canine visceral leishmaniasis serology are mostly IgA immune complexes |
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