Spectroscopic analysis of coenzyme binding to betaine aldehyde dehydrogenase dependent on potassium

Glycine betaine is the main osmolyte synthesized and accumulated in mammalian renal cells. Glycine betaine synthesis is catalyzed by the enzyme betaine aldehyde dehydrogenase (BADH) using NAD+ as the coenzyme. Previous studies have shown that porcine kidney betaine aldehyde dehydrogenase (pkBADH) bi...

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Veröffentlicht in:	Luminescence (Chichester, England) England), 2021-11, Vol.36 (7), p.1733-1742
Hauptverfasser:	Muñoz‐Bacasehua, César, Rosas‐Rodríguez, Jesús A., López‐Zavala, Alexis Alonso, Valenzuela‐Soto, Elisa M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldehyde dehydrogenase Aldehydes Analysis betaine aldehyde dehydrogenase Binding sites Chemical synthesis coenzyme binding Coenzymes Complex formation Conformation Data analysis Dehydrogenase Dehydrogenases Emission analysis Enzymes Fluorescence Glycine Glycine (amino acid) Glycine betaine Kidneys NAD negative cooperativity Potassium Protein structure protein structure model Tertiary Tertiary structure Thermal stability Tryptophan
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