Mapping Surface Charge Distribution of Single-Cell via Charged Nanoparticle
Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanopartic...
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| Veröffentlicht in: | Cells (Basel, Switzerland) Switzerland), 2021-06, Vol.10 (6), p.1519 |
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| creator | Ouyang, Leixin Shaik, Rubia Xu, Ruiting Zhang, Ge Zhe, Jiang |
| description | Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields. |
| doi_str_mv | 10.3390/cells10061519 |
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Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields.</description><identifier>ISSN: 2073-4409</identifier><identifier>EISSN: 2073-4409</identifier><identifier>DOI: 10.3390/cells10061519</identifier><identifier>PMID: 34208707</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Cell surface ; cell surface charge ; charged nanoparticle ; Electrostatic properties ; Endothelial cells ; Light intensity ; Light scattering ; Mapping ; Microscopy ; Nanoparticles ; Surface charge ; surface charge mapping ; Umbilical vein</subject><ispartof>Cells (Basel, Switzerland), 2021-06, Vol.10 (6), p.1519</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. 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| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Cell surface cell surface charge charged nanoparticle Electrostatic properties Endothelial cells Light intensity Light scattering Mapping Microscopy Nanoparticles Surface charge surface charge mapping Umbilical vein |
| title | Mapping Surface Charge Distribution of Single-Cell via Charged Nanoparticle |
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