Effect of gamma irradiation on nucleolar activity in root tip cells of tetraploid Triticum turgidum ssp. durum L
Ionizing irradiation induces positive or negative changes in plant growth (M1) depending on the amount of irradiation applied to seeds or plant parts. The effect of 50–350 Gy gamma irradiation of kernels on nucleolar activity, as an indicator of metabolic activity, in root tip cells of tetraploid wh...
Gespeichert in:
| Veröffentlicht in: | Protoplasma 2022-03, Vol.259 (2), p.453-468 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 468 |
|---|---|
| container_issue | 2 |
| container_start_page | 453 |
| container_title | Protoplasma |
| container_volume | 259 |
| creator | von Well, Eben Booyse, Mardé Fossey, Annabel |
| description | Ionizing irradiation induces positive or negative changes in plant growth (M1) depending on the amount of irradiation applied to seeds or plant parts. The effect of 50–350 Gy gamma irradiation of kernels on nucleolar activity, as an indicator of metabolic activity, in root tip cells of tetraploid wheat
Triticum turgidum
ssp.
durum
L. cv. Orania (AABB) was investigated. The number of nucleoli present in nuclei and micronuclei as well as the mitotic index in the different irradiation dosages was used as an indicator of the cells entering mitosis, the chromosomes with nucleolar organizer regions that are active as well as chromosome doubling in the event of unsuccessful mitotic division. Nucleolar activity was investigated from 17.5 to 47.5 h after the onset of imbibition to study the first mitotic division and its consequences on the cells that were in G
2
and G
1
phases at the time of gamma irradiation. Untreated material produced a maximum of four nucleoli formed by the nucleolar organizing regions (NORs) on chromosomes 1B and 6B. In irradiated material, additional nucleoli were noted that are due to the activation of the NORs on chromosome 1A in micronuclei. The onset of mitosis was highly significantly retarded in comparison to the control due to checkpoints in the G
2
phase for the repairing of damaged DNA. This study is the first to report on the appearance of nucleoli in micronuclei as well as activation of NORs in the micronuclei that are inactive in the nucleus and the effect of chromosome doubling on nucleolar activity in the event of unsuccessful mitotic division. |
| doi_str_mv | 10.1007/s00709-021-01684-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2546975956</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2546975956</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-46f86160adef90f9c1cc9c3d9768bdee427f9ccbb76dd1f755b45573d56b98d13</originalsourceid><addsrcrecordid>eNp9kU1rHSEUhqU0NLdp_0AXReimm0k9Ouq4LCH9gAvdJJCdOH5cDDPjVJ1C_n28uWkLXRRED8fnfc-BF6F3QC6BEPmptIuojlDoCIih7_oXaAcCeCeA0JdoRwhjHQzs7hy9LuWeEMIp4a_QOetBAVC6Q-t1CN5WnAI-mHk2OOZsXDQ1pgW3s2x28mkyGRtb469YH3BccE6p4hpXbP00laO4-prNOqXo8E2ONdptxnXLh-haUcp6id2WW7l_g86CmYp_-_xeoNsv1zdX37r9j6_frz7vO8skr10vwiBAEON8UCQoC9Yqy5ySYhid9z2VrWnHUQrnIEjOx55zyRwXoxocsAv08eS75vRz86XqOZbjumbxaSua8l4oyRUXDf3wD3qftry07TQVDNhAJNBG0RNlcyol-6DXHGeTHzQQfcxDn_LQLQ_9lIfum-j9s_U2zt79kfwOoAHsBJT2tRx8_jv7P7aPBJSWrA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2631380712</pqid></control><display><type>article</type><title>Effect of gamma irradiation on nucleolar activity in root tip cells of tetraploid Triticum turgidum ssp. durum L</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>von Well, Eben ; Booyse, Mardé ; Fossey, Annabel</creator><creatorcontrib>von Well, Eben ; Booyse, Mardé ; Fossey, Annabel</creatorcontrib><description>Ionizing irradiation induces positive or negative changes in plant growth (M1) depending on the amount of irradiation applied to seeds or plant parts. The effect of 50–350 Gy gamma irradiation of kernels on nucleolar activity, as an indicator of metabolic activity, in root tip cells of tetraploid wheat
Triticum turgidum
ssp.
durum
L. cv. Orania (AABB) was investigated. The number of nucleoli present in nuclei and micronuclei as well as the mitotic index in the different irradiation dosages was used as an indicator of the cells entering mitosis, the chromosomes with nucleolar organizer regions that are active as well as chromosome doubling in the event of unsuccessful mitotic division. Nucleolar activity was investigated from 17.5 to 47.5 h after the onset of imbibition to study the first mitotic division and its consequences on the cells that were in G
2
and G
1
phases at the time of gamma irradiation. Untreated material produced a maximum of four nucleoli formed by the nucleolar organizing regions (NORs) on chromosomes 1B and 6B. In irradiated material, additional nucleoli were noted that are due to the activation of the NORs on chromosome 1A in micronuclei. The onset of mitosis was highly significantly retarded in comparison to the control due to checkpoints in the G
2
phase for the repairing of damaged DNA. This study is the first to report on the appearance of nucleoli in micronuclei as well as activation of NORs in the micronuclei that are inactive in the nucleus and the effect of chromosome doubling on nucleolar activity in the event of unsuccessful mitotic division.</description><identifier>ISSN: 0033-183X</identifier><identifier>EISSN: 1615-6102</identifier><identifier>DOI: 10.1007/s00709-021-01684-4</identifier><identifier>PMID: 34191122</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Biomedical and Life Sciences ; Cell Biology ; Cell Nucleolus ; Cell Nucleus ; Chromosomes ; G2 phase ; Life Sciences ; Meristem ; Micronuclei ; Mitosis ; Nuclei ; Nucleoli ; Nucleolus Organizer Region ; Original Article ; Plant Sciences ; Seeds ; Tetraploidy ; Triticum - genetics ; Triticum - metabolism ; Triticum turgidum ; Zoology ; γ Radiation</subject><ispartof>Protoplasma, 2022-03, Vol.259 (2), p.453-468</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-46f86160adef90f9c1cc9c3d9768bdee427f9ccbb76dd1f755b45573d56b98d13</citedby><cites>FETCH-LOGICAL-c375t-46f86160adef90f9c1cc9c3d9768bdee427f9ccbb76dd1f755b45573d56b98d13</cites><orcidid>0000-0001-6360-9743</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00709-021-01684-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00709-021-01684-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27906,27907,41470,42539,51301</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34191122$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>von Well, Eben</creatorcontrib><creatorcontrib>Booyse, Mardé</creatorcontrib><creatorcontrib>Fossey, Annabel</creatorcontrib><title>Effect of gamma irradiation on nucleolar activity in root tip cells of tetraploid Triticum turgidum ssp. durum L</title><title>Protoplasma</title><addtitle>Protoplasma</addtitle><addtitle>Protoplasma</addtitle><description>Ionizing irradiation induces positive or negative changes in plant growth (M1) depending on the amount of irradiation applied to seeds or plant parts. The effect of 50–350 Gy gamma irradiation of kernels on nucleolar activity, as an indicator of metabolic activity, in root tip cells of tetraploid wheat
Triticum turgidum
ssp.
durum
L. cv. Orania (AABB) was investigated. The number of nucleoli present in nuclei and micronuclei as well as the mitotic index in the different irradiation dosages was used as an indicator of the cells entering mitosis, the chromosomes with nucleolar organizer regions that are active as well as chromosome doubling in the event of unsuccessful mitotic division. Nucleolar activity was investigated from 17.5 to 47.5 h after the onset of imbibition to study the first mitotic division and its consequences on the cells that were in G
2
and G
1
phases at the time of gamma irradiation. Untreated material produced a maximum of four nucleoli formed by the nucleolar organizing regions (NORs) on chromosomes 1B and 6B. In irradiated material, additional nucleoli were noted that are due to the activation of the NORs on chromosome 1A in micronuclei. The onset of mitosis was highly significantly retarded in comparison to the control due to checkpoints in the G
2
phase for the repairing of damaged DNA. This study is the first to report on the appearance of nucleoli in micronuclei as well as activation of NORs in the micronuclei that are inactive in the nucleus and the effect of chromosome doubling on nucleolar activity in the event of unsuccessful mitotic division.</description><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Cell Nucleolus</subject><subject>Cell Nucleus</subject><subject>Chromosomes</subject><subject>G2 phase</subject><subject>Life Sciences</subject><subject>Meristem</subject><subject>Micronuclei</subject><subject>Mitosis</subject><subject>Nuclei</subject><subject>Nucleoli</subject><subject>Nucleolus Organizer Region</subject><subject>Original Article</subject><subject>Plant Sciences</subject><subject>Seeds</subject><subject>Tetraploidy</subject><subject>Triticum - genetics</subject><subject>Triticum - metabolism</subject><subject>Triticum turgidum</subject><subject>Zoology</subject><subject>γ Radiation</subject><issn>0033-183X</issn><issn>1615-6102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1rHSEUhqU0NLdp_0AXReimm0k9Ouq4LCH9gAvdJJCdOH5cDDPjVJ1C_n28uWkLXRRED8fnfc-BF6F3QC6BEPmptIuojlDoCIih7_oXaAcCeCeA0JdoRwhjHQzs7hy9LuWeEMIp4a_QOetBAVC6Q-t1CN5WnAI-mHk2OOZsXDQ1pgW3s2x28mkyGRtb469YH3BccE6p4hpXbP00laO4-prNOqXo8E2ONdptxnXLh-haUcp6id2WW7l_g86CmYp_-_xeoNsv1zdX37r9j6_frz7vO8skr10vwiBAEON8UCQoC9Yqy5ySYhid9z2VrWnHUQrnIEjOx55zyRwXoxocsAv08eS75vRz86XqOZbjumbxaSua8l4oyRUXDf3wD3qftry07TQVDNhAJNBG0RNlcyol-6DXHGeTHzQQfcxDn_LQLQ_9lIfum-j9s_U2zt79kfwOoAHsBJT2tRx8_jv7P7aPBJSWrA</recordid><startdate>20220301</startdate><enddate>20220301</enddate><creator>von Well, Eben</creator><creator>Booyse, Mardé</creator><creator>Fossey, Annabel</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6360-9743</orcidid></search><sort><creationdate>20220301</creationdate><title>Effect of gamma irradiation on nucleolar activity in root tip cells of tetraploid Triticum turgidum ssp. durum L</title><author>von Well, Eben ; Booyse, Mardé ; Fossey, Annabel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-46f86160adef90f9c1cc9c3d9768bdee427f9ccbb76dd1f755b45573d56b98d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Cell Nucleolus</topic><topic>Cell Nucleus</topic><topic>Chromosomes</topic><topic>G2 phase</topic><topic>Life Sciences</topic><topic>Meristem</topic><topic>Micronuclei</topic><topic>Mitosis</topic><topic>Nuclei</topic><topic>Nucleoli</topic><topic>Nucleolus Organizer Region</topic><topic>Original Article</topic><topic>Plant Sciences</topic><topic>Seeds</topic><topic>Tetraploidy</topic><topic>Triticum - genetics</topic><topic>Triticum - metabolism</topic><topic>Triticum turgidum</topic><topic>Zoology</topic><topic>γ Radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>von Well, Eben</creatorcontrib><creatorcontrib>Booyse, Mardé</creatorcontrib><creatorcontrib>Fossey, Annabel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Psychology</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Protoplasma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>von Well, Eben</au><au>Booyse, Mardé</au><au>Fossey, Annabel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of gamma irradiation on nucleolar activity in root tip cells of tetraploid Triticum turgidum ssp. durum L</atitle><jtitle>Protoplasma</jtitle><stitle>Protoplasma</stitle><addtitle>Protoplasma</addtitle><date>2022-03-01</date><risdate>2022</risdate><volume>259</volume><issue>2</issue><spage>453</spage><epage>468</epage><pages>453-468</pages><issn>0033-183X</issn><eissn>1615-6102</eissn><abstract>Ionizing irradiation induces positive or negative changes in plant growth (M1) depending on the amount of irradiation applied to seeds or plant parts. The effect of 50–350 Gy gamma irradiation of kernels on nucleolar activity, as an indicator of metabolic activity, in root tip cells of tetraploid wheat
Triticum turgidum
ssp.
durum
L. cv. Orania (AABB) was investigated. The number of nucleoli present in nuclei and micronuclei as well as the mitotic index in the different irradiation dosages was used as an indicator of the cells entering mitosis, the chromosomes with nucleolar organizer regions that are active as well as chromosome doubling in the event of unsuccessful mitotic division. Nucleolar activity was investigated from 17.5 to 47.5 h after the onset of imbibition to study the first mitotic division and its consequences on the cells that were in G
2
and G
1
phases at the time of gamma irradiation. Untreated material produced a maximum of four nucleoli formed by the nucleolar organizing regions (NORs) on chromosomes 1B and 6B. In irradiated material, additional nucleoli were noted that are due to the activation of the NORs on chromosome 1A in micronuclei. The onset of mitosis was highly significantly retarded in comparison to the control due to checkpoints in the G
2
phase for the repairing of damaged DNA. This study is the first to report on the appearance of nucleoli in micronuclei as well as activation of NORs in the micronuclei that are inactive in the nucleus and the effect of chromosome doubling on nucleolar activity in the event of unsuccessful mitotic division.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>34191122</pmid><doi>10.1007/s00709-021-01684-4</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0001-6360-9743</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0033-183X |
| ispartof | Protoplasma, 2022-03, Vol.259 (2), p.453-468 |
| issn | 0033-183X 1615-6102 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2546975956 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Biomedical and Life Sciences Cell Biology Cell Nucleolus Cell Nucleus Chromosomes G2 phase Life Sciences Meristem Micronuclei Mitosis Nuclei Nucleoli Nucleolus Organizer Region Original Article Plant Sciences Seeds Tetraploidy Triticum - genetics Triticum - metabolism Triticum turgidum Zoology γ Radiation |
| title | Effect of gamma irradiation on nucleolar activity in root tip cells of tetraploid Triticum turgidum ssp. durum L |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T10%3A52%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20gamma%20irradiation%20on%20nucleolar%20activity%20in%20root%20tip%20cells%20of%20tetraploid%20Triticum%20turgidum%20ssp.%20durum%20L&rft.jtitle=Protoplasma&rft.au=von%20Well,%20Eben&rft.date=2022-03-01&rft.volume=259&rft.issue=2&rft.spage=453&rft.epage=468&rft.pages=453-468&rft.issn=0033-183X&rft.eissn=1615-6102&rft_id=info:doi/10.1007/s00709-021-01684-4&rft_dat=%3Cproquest_cross%3E2546975956%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2631380712&rft_id=info:pmid/34191122&rfr_iscdi=true |