Acute hypoxic exposure: Effect on hemocyte functional parameters and antioxidant potential in gills of the pacific oyster, Crassostrea gigas

Bivalve mollusks are frequently subjected to fluctuations of dissolved oxygen concentration in the environment which can represent a significant threat to bivalve antioxidant status. In this work the effects of hypoxia on hemocyte reactive oxygen species (ROS) production and level of mitochondrial potential as well as the activity and expression level of catalase (CAT) and superoxide dismutase (SOD) in gills of Crassostrea gigas were investigated after 24 h and 72 h exposure. 24 h hypoxia promoted an increase of mitochondrial membrane potential in agranulocytes and induced ROS accumulation in granulocytes. 72 h exposure substantially decreased hemocyte mitochondrial potential and intracellular ROS level in all hemocyte types. No significant changes in the activity of CAT in gills were observed following both 24 h and 72 h exposure periods compared to control. SOD activity in gills decreased after 72 h exposure to hypoxia but did not change under 24 h hypoxia. Significant up-regulation of SOD gene and no changes in expression level of CAT were observed in all experimental groups. The results indicate an overall shift in antioxidant status in gills and hemocytes of the Pacific oyster that may act as compensatory mechanisms to maintain redox homeostasis after a short-term (24 h) exposure and represent the occurrence of oxidative stress conditions at the end of 72 h hypoxia. •24 h hypoxia promoted an increase mitochondrial membrane potential in agranulocytes and ROS production by granulocytes.•72 h exposure decreased hemocyte mitochondrial potential and intracellular ROS level.•Superoxide dismutase activity decreased after 72 h hypoxia but did not change under 24 h hypoxia.•Hypoxia promoted upregulation of superoxide dismutase gene.•No changes in the activity and expression level of catalase were observed under hypoxia.