Point-of-Care Test Paper for Exhaled Breath Aldehyde Analysis via Mass Spectrometry
Volatile organic compounds (VOCs) from exhaled breath (EB) are considered to be promising biomarkers for lung diseases. A convenient and sensitive point-of-care (POC) testing method for EB VOCs is essential. Here, we developed a POC test paper for the analysis of EB aldehydes, which are potential bi...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2021-07, Vol.93 (26), p.9158-9165 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Li, Zhengzhou Li, Yuze Zhan, Lingpeng Meng, Lingwei Huang, Xi Wang, Tie Li, Yafeng Nie, Zongxiu |
| description | Volatile organic compounds (VOCs) from exhaled breath (EB) are considered to be promising biomarkers for lung diseases. A convenient and sensitive point-of-care (POC) testing method for EB VOCs is essential. Here, we developed a POC test paper for the analysis of EB aldehydes, which are potential biomarkers for lung cancer. A probe molecule, 4-aminothiophenol (4-ATP), was anchored on a paper substrate to specifically capture gas-phase aldehydes through the Schiff base reaction. Meanwhile, thin-film reaction acceleration was utilized to increase capture efficiency. By directly coupling the test paper to a mass spectrometer through paper spray, high sensitivity (0.1 ppt) and a wide quantification linear range (from 10 ppt to 1 ppm) were obtained. Analysis of EB from lung cancer patients with the test paper showed a significant increase in several reported aldehyde markers compared to EB from healthy volunteers, indicating the potential of this method for sensitive, low-cost, and convenient lung cancer screening and diagnosis. |
| doi_str_mv | 10.1021/acs.analchem.1c01011 |
| format | Article |
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A convenient and sensitive point-of-care (POC) testing method for EB VOCs is essential. Here, we developed a POC test paper for the analysis of EB aldehydes, which are potential biomarkers for lung cancer. A probe molecule, 4-aminothiophenol (4-ATP), was anchored on a paper substrate to specifically capture gas-phase aldehydes through the Schiff base reaction. Meanwhile, thin-film reaction acceleration was utilized to increase capture efficiency. By directly coupling the test paper to a mass spectrometer through paper spray, high sensitivity (0.1 ppt) and a wide quantification linear range (from 10 ppt to 1 ppm) were obtained. Analysis of EB from lung cancer patients with the test paper showed a significant increase in several reported aldehyde markers compared to EB from healthy volunteers, indicating the potential of this method for sensitive, low-cost, and convenient lung cancer screening and diagnosis.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.1c01011</identifier><language>eng</language><publisher>Washington: American Chemical Society</publisher><subject>Acceleration ; Aldehydes ; Biomarkers ; Cancer screening ; Chemistry ; Imines ; Lung cancer ; Lung diseases ; Mass spectrometry ; Mass spectroscopy ; Medical screening ; Organic compounds ; Sensitivity analysis ; Substrates ; Thin films ; VOCs ; Volatile organic compounds</subject><ispartof>Analytical chemistry (Washington), 2021-07, Vol.93 (26), p.9158-9165</ispartof><rights>2021 American Chemical Society</rights><rights>Copyright American Chemical Society Jul 6, 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a353t-a1897527996e08ae32c67faaff8b498585a1759050c8dca927a81a87cb4503e93</citedby><cites>FETCH-LOGICAL-a353t-a1897527996e08ae32c67faaff8b498585a1759050c8dca927a81a87cb4503e93</cites><orcidid>0000-0001-5965-6520 ; 0000-0001-8514-6348</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.1c01011$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.1c01011$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids></links><search><creatorcontrib>Li, Zhengzhou</creatorcontrib><creatorcontrib>Li, Yuze</creatorcontrib><creatorcontrib>Zhan, Lingpeng</creatorcontrib><creatorcontrib>Meng, Lingwei</creatorcontrib><creatorcontrib>Huang, Xi</creatorcontrib><creatorcontrib>Wang, Tie</creatorcontrib><creatorcontrib>Li, Yafeng</creatorcontrib><creatorcontrib>Nie, Zongxiu</creatorcontrib><title>Point-of-Care Test Paper for Exhaled Breath Aldehyde Analysis via Mass Spectrometry</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Volatile organic compounds (VOCs) from exhaled breath (EB) are considered to be promising biomarkers for lung diseases. A convenient and sensitive point-of-care (POC) testing method for EB VOCs is essential. Here, we developed a POC test paper for the analysis of EB aldehydes, which are potential biomarkers for lung cancer. A probe molecule, 4-aminothiophenol (4-ATP), was anchored on a paper substrate to specifically capture gas-phase aldehydes through the Schiff base reaction. Meanwhile, thin-film reaction acceleration was utilized to increase capture efficiency. By directly coupling the test paper to a mass spectrometer through paper spray, high sensitivity (0.1 ppt) and a wide quantification linear range (from 10 ppt to 1 ppm) were obtained. Analysis of EB from lung cancer patients with the test paper showed a significant increase in several reported aldehyde markers compared to EB from healthy volunteers, indicating the potential of this method for sensitive, low-cost, and convenient lung cancer screening and diagnosis.</description><subject>Acceleration</subject><subject>Aldehydes</subject><subject>Biomarkers</subject><subject>Cancer screening</subject><subject>Chemistry</subject><subject>Imines</subject><subject>Lung cancer</subject><subject>Lung diseases</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical screening</subject><subject>Organic compounds</subject><subject>Sensitivity analysis</subject><subject>Substrates</subject><subject>Thin films</subject><subject>VOCs</subject><subject>Volatile organic compounds</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kD1PwzAQhi0EEuXjHzBYYmFJOTtx4oylKh9SEZVa5ujqXJRUSV3sFJF_T6IWBgamG-557-Nh7EbAWIAU92j8GLdYm5KasTAgQIgTNhJKQhBrLU_ZCADCQCYA5-zC-w30BIh4xJYLW23bwBbBFB3xFfmWL3BHjhfW8dlXiTXl_MERtiWf1DmVXU580i_rfOX5Z4X8Fb3nyx2Z1tmGWtddsbMCa0_Xx3rJ3h9nq-lzMH97eplO5gGGKmwDFDpNlEzSNCbQSKE0cVIgFoVeR6lWWqFIVAoKjM4NpjJBLVAnZh0pCCkNL9ndYe7O2Y99f3nWVN5QXeOW7N5nUkWRTnQUDujtH3Rj967_YqCUFCKNIeyp6EAZZ713VGQ7VzXoukxANpjOetPZj-nsaLqPwSE2dH_n_hv5BupIg04</recordid><startdate>20210706</startdate><enddate>20210706</enddate><creator>Li, Zhengzhou</creator><creator>Li, Yuze</creator><creator>Zhan, Lingpeng</creator><creator>Meng, Lingwei</creator><creator>Huang, Xi</creator><creator>Wang, Tie</creator><creator>Li, Yafeng</creator><creator>Nie, Zongxiu</creator><general>American Chemical Society</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5965-6520</orcidid><orcidid>https://orcid.org/0000-0001-8514-6348</orcidid></search><sort><creationdate>20210706</creationdate><title>Point-of-Care Test Paper for Exhaled Breath Aldehyde Analysis via Mass Spectrometry</title><author>Li, Zhengzhou ; Li, Yuze ; Zhan, Lingpeng ; Meng, Lingwei ; Huang, Xi ; Wang, Tie ; Li, Yafeng ; Nie, Zongxiu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a353t-a1897527996e08ae32c67faaff8b498585a1759050c8dca927a81a87cb4503e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acceleration</topic><topic>Aldehydes</topic><topic>Biomarkers</topic><topic>Cancer screening</topic><topic>Chemistry</topic><topic>Imines</topic><topic>Lung cancer</topic><topic>Lung diseases</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medical screening</topic><topic>Organic compounds</topic><topic>Sensitivity analysis</topic><topic>Substrates</topic><topic>Thin films</topic><topic>VOCs</topic><topic>Volatile organic compounds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Zhengzhou</creatorcontrib><creatorcontrib>Li, Yuze</creatorcontrib><creatorcontrib>Zhan, Lingpeng</creatorcontrib><creatorcontrib>Meng, Lingwei</creatorcontrib><creatorcontrib>Huang, Xi</creatorcontrib><creatorcontrib>Wang, Tie</creatorcontrib><creatorcontrib>Li, Yafeng</creatorcontrib><creatorcontrib>Nie, Zongxiu</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Zhengzhou</au><au>Li, Yuze</au><au>Zhan, Lingpeng</au><au>Meng, Lingwei</au><au>Huang, Xi</au><au>Wang, Tie</au><au>Li, Yafeng</au><au>Nie, Zongxiu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Point-of-Care Test Paper for Exhaled Breath Aldehyde Analysis via Mass Spectrometry</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2021-07-06</date><risdate>2021</risdate><volume>93</volume><issue>26</issue><spage>9158</spage><epage>9165</epage><pages>9158-9165</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Volatile organic compounds (VOCs) from exhaled breath (EB) are considered to be promising biomarkers for lung diseases. A convenient and sensitive point-of-care (POC) testing method for EB VOCs is essential. Here, we developed a POC test paper for the analysis of EB aldehydes, which are potential biomarkers for lung cancer. A probe molecule, 4-aminothiophenol (4-ATP), was anchored on a paper substrate to specifically capture gas-phase aldehydes through the Schiff base reaction. Meanwhile, thin-film reaction acceleration was utilized to increase capture efficiency. By directly coupling the test paper to a mass spectrometer through paper spray, high sensitivity (0.1 ppt) and a wide quantification linear range (from 10 ppt to 1 ppm) were obtained. Analysis of EB from lung cancer patients with the test paper showed a significant increase in several reported aldehyde markers compared to EB from healthy volunteers, indicating the potential of this method for sensitive, low-cost, and convenient lung cancer screening and diagnosis.</abstract><cop>Washington</cop><pub>American Chemical Society</pub><doi>10.1021/acs.analchem.1c01011</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-5965-6520</orcidid><orcidid>https://orcid.org/0000-0001-8514-6348</orcidid></addata></record> |
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| source | American Chemical Society Journals |
| subjects | Acceleration Aldehydes Biomarkers Cancer screening Chemistry Imines Lung cancer Lung diseases Mass spectrometry Mass spectroscopy Medical screening Organic compounds Sensitivity analysis Substrates Thin films VOCs Volatile organic compounds |
| title | Point-of-Care Test Paper for Exhaled Breath Aldehyde Analysis via Mass Spectrometry |
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